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Gene information
General information
TC002569 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 650043..654505
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002569
    iBeetle screen iB_00403
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    remarks: animals lost, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002569 RA
      MGRPGFSPG GRGGGGRGG GGFGGRGGR GGGGRGGGG 
      GGRGGFGRG GGGGGGGRP SFGRGGGGG RGGRGGGRG 
      GGGGRGGFK GGKTVVIEP HRHEGVFIA KGKEDALVT 
      LNLVPGSEV YGEKRISVE TDGNKVEYR VWNPFRSKL 
      AAAILGGVD QIHMPPGSK VLYLGAASG TTVSHVSDV 
      VGPEGLVYA VEFSHRSGR DLINVAKKR TNIIPIIED 
      ARHPHKYRM LVGMVDTVF ADVAQPDQA RIVALNAQY 
      FLKNGGHFV ISIKASCID STAQPEAVF ASEVKRLQA 
      DQLKPQEQI TLEPYERDH AVVVGVFRP PPKKQQ
    CDS sequence
    • >TC002569 RA
      atgggtaga ccaggattc tcgccgggt ggccgcgga 
      ggaggcggt cgaggcggc ggtggtttt ggtggtcgt 
      ggaggccgc gggggtgga ggccgtggc ggtggaggc 
      ggcggacgt ggtggtttt ggcagggga ggaggaggc 
      ggtggcggc ggtcgcccg agttttggt agaggtgga 
      ggcggcggt cgtggaggc cgcggggga ggaagaggc 
      ggcggcggc ggccgaggc gggttcaaa gggggcaag 
      acagtggta attgaaccg catagacac gagggcgtg 
      tttatcgcc aaaggcaaa gaggatgct ttagttact 
      ttgaatctt gttccgggg tctgaggtt tatggagag 
      aagaggata tcagttgag actgatgga aataaagtc 
      gaatacaga gtttggaac cccttcaga agcaaacta 
      gccgctgct atcttgggc ggagtcgac caaatccac 
      atgccccca ggaagcaaa gtattatat ttgggggct 
      gcgtctggc accaccgtg tcccacgtc tctgatgtt 
      gtggggcct gaaggccta gtctacgca gtggaattt 
      tcacacaga tcaggacgc gatctaata aacgtggcc 
      aaaaagcga accaatatt attccaatc attgaagat 
      gccagacac ccgcataaa tatagaatg ttggttgga 
      atggttgac actgttttt gcagatgtt gcccagcct 
      gaccaagct agaatagtg gccctaaac gcacagtac 
      tttttgaaa aatgggggc catttcgtc atttctatt 
      aaagcctcg tgtattgat tcaacagcg caacctgaa 
      gccgttttt gcgtccgaa gttaaaagg ttgcaagcg 
      gatcaactg aaacctcag gaacagatc actttggaa 
      ccttacgag cgggaccat gcggttgtt gtgggggtg 
      tttagaccg ccccccaag aagcagcaa tga
    mRNA sequence
    • >TC002569 RA
      agggatttt cgtttcttc gatgcgagt ggtgcgtca 
      aattgaggt tagattctc aaaaccgcg tgttttgta 
      catacagcg tgttcactg aacgcgtaa acaaacgta 
      ttttttaat tgaaaaaga aaacacaat gggtagacc 
      aggattctc gccgggtgg ccgcggagg aggcggtcg 
      aggcggcgg tggttttgg tggtcgtgg aggccgcgg 
      gggtggagg ccgtggcgg tggaggcgg cggacgtgg 
      tggttttgg caggggagg aggaggcgg tggcggcgg 
      tcgcccgag ttttggtag aggtggagg cggcggtcg 
      tggaggccg cgggggagg aagaggcgg cggcggcgg 
      ccgaggcgg gttcaaagg gggcaagac agtggtaat 
      tgaaccgca tagacacga gggcgtgtt tatcgccaa 
      aggcaaaga ggatgcttt agttacttt gaatcttgt 
      tccggggtc tgaggttta tggagagaa gaggatatc 
      agttgagac tgatggaaa taaagtcga atacagagt 
      ttggaaccc cttcagaag caaactagc cgctgctat 
      cttgggcgg agtcgacca aatccacat gcccccagg 
      aagcaaagt attatattt gggggctgc gtctggcac 
      caccgtgtc ccacgtctc tgatgttgt ggggcctga 
      aggcctagt ctacgcagt ggaattttc acacagatc 
      aggacgcga tctaataaa cgtggccaa aaagcgaac 
      caatattat tccaatcat tgaagatgc cagacaccc 
      gcataaata tagaatgtt ggttggaat ggttgacac 
      tgtttttgc agatgttgc ccagcctga ccaagctag 
      aatagtggc cctaaacgc acagtactt tttgaaaaa 
      tgggggcca tttcgtcat ttctattaa agcctcgtg 
      tattgattc aacagcgca acctgaagc cgtttttgc 
      gtccgaagt taaaaggtt gcaagcgga tcaactgaa 
      acctcagga acagatcac tttggaacc ttacgagcg 
      ggaccatgc ggttgttgt gggggtgtt tagaccgcc 
      ccccaagaa gcagcaatg attcttttt tgtatcatc 
      taagaaata gcatttttc ctttcgttc cgttttaat 
      tttaataaa tatatatca ataaa