Status
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Gene information
General information
TC002561 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23353492..23389849
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002561
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00402

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002561 RA
      MMRYSEIPR DISGDSLTA LKIPESRKQ DSFRTSDID 
      GDSVSLSSS TSHDRFPSE IRFRKISTP AVSSGPGPP 
      FTYYNYGSL ASVPAHIKT QSLPSNART KNYSCAIFD 
      ILRISPEDF ASQLTLLDL PVFLNIQPD ELTSCAWNK 
      KNKLTMAPN VVAFTRRFN HVSFWTVQE ILSGPTPKQ 
      RAEILAFFV RIAKKLYDL NNLHSLFAM ISALQSASI 
      YRLSKTWTC LSKKDKQTF DKLAEVFSD ADNWSNLRR 
      HIESLKLPC IPYLGLYLT DLVYIDMAH PHSGGLESQ 
      QRTLKMNNI LRVISNYQH SDYSHLTAI PHIQDYLNS 
      IRYIEELQK FVEDDQYKL SLKLEPLSP APSSSSCSS 
      KESVIADAA AIASLNLSP AKASSGSLR LHAVTSGSK 
      FIPGHRKCR SLGTNIFSK SHQVGSFDP KDLPKTLHL 
      LDDSELEDS SRNLHLLHA DLPSPTIDL SLQASQNTA 
      ENSMLLTRL AEDSLGTED QVCTMQGCV RRKTVLKEG 
      RKPTVTSWQ RYWLQIWTT SLVYFSPKS FKGHQRSDF 
      KREPCKLVP LMGCQIILG DNSVQQDCF QIIDHHRGN 
      IYKYRAGTK SLAEKWCRH LQQAANGEQ TPLPSNLMS 
      FE
    • >TC002561 RB
      MMRYSEIPR DISGDSLTA LKIPESRKQ DSFRTSDID 
      GDSVSLSSS TSHDRFPSE IRFRKISTP AVSSGPGPP 
      FTYYNYGIS PEDFASQLT LLDLPVFLN IQPDELTSC 
      AWNKKNKLT MAPNVVAFT RRFNHVSFW TVQEILSGP 
      TPKQRAEIL AFFVRIAKK LYDLNNLHS LFAMISALQ 
      SASIYRLSK TWTCLSKKD KQTFDKLAE VFSDADNWS 
      NLRRHIESL KLPCIPYLG LYLTDLVYI DMAHPHSGG 
      LESQQRTLK MNNILRVIS NYQHSDYSH LTAIPHIQD 
      YLNSIRYIE ELQKFVEDD QYKLSLKLE PLSPAPSSS 
      SCSSKESVI ADAAAIASL NLSPAKASS GSLRLHAVT 
      SGSKFIPGH RKCRSLGTN IFSKSHQVG SFDPKDLPK 
      TLHLLDDSE LEDSSRNLH LLHADLPSP TIDLSLQAS 
      QNTAENSML LTRLAEDSL GTEDQVCTM QGCVRRKTV 
      LKEGRKPTV TSWQRYWLQ IWTTSLVYF SPKSFKGHQ 
      RSDFKREPC KLVPLMGCQ IILGDNSVQ QDCFQIIDH 
      HRGNIYKYR AGTKSLAEK WCRHLQQAA NGEQTPLPS 
      NLMSFE
    CDS sequence
    • >TC002561 RA
      atgatgcgc tactccgaa ataccaagg gatatttca 
      ggagacagt ctaaccgca ctcaaaata ccggagagt 
      cgaaagcag gatagcttt aggacctcc gatatcgat 
      ggcgattcg gtttcactg tcttcgtct acgagccat 
      gacaggttt ccttcagaa attaggttt aggaaaata 
      tcaacaccg gctgtctcc agtggaccg ggaccaccc 
      tttacctac tataactac ggttctctc gcatctgta 
      cctgcccat atcaaaacc caaagcttg ccgagcaat 
      gcccgaacc aaaaactac agctgtgct attttcgac 
      atactcagg atatcaccg gaagacttc gccagtcaa 
      ttgacattg ctcgattta cctgtgttc ctcaacatc 
      caacccgac gaactcacc agctgtgca tggaataag 
      aaaaacaag ttgaccatg gcgcccaat gttgttgct 
      ttcacgaga cgattcaat catgtgagc ttctggacg 
      gtgcaagaa atactcagc ggaccgaca cccaaacaa 
      agagcagaa atactcgcg ttttttgtt cgaatagcg 
      aagaagttg tacgatttg aataatttg cattcgtta 
      tttgcaatg atttcagcc ttacaaagt gcctcgatt 
      tacagatta tcaaagacg tggacttgt ttgtctaaa 
      aaagataaa cagactttt gacaagctg gccgaggtg 
      ttttcggat gctgataat tggtcgaat ctcaggcga 
      catattgaa agtttgaaa cttccttgc ataccctat 
      ttaggtttg tatctgact gatcttgtt tacattgac 
      atggcccat cctcattcg ggtggtttg gaatcgcag 
      caaaggact ctcaaaatg aacaatatt ttacgcgta 
      atctcgaat tatcagcat tctgattat tctcacttg 
      actgcaata cctcacatt caagactat ttgaactca 
      atcaggtac atcgaggaa cttcaaaaa ttcgtcgaa 
      gatgatcag tacaaactt tcattgaaa ctggaacca 
      ctttcgccg gcgccgagt tcctcaagt tgcagctca 
      aaagaatcg gtgattgcc gatgcagcc gcaatcgca 
      tcattaaat ctctcgcct gcaaaggct tccagcgga 
      tcattacgt cttcacgcc gttacatca ggaagtaaa 
      ttcataccg ggtcacaga aaatgccgc agtttagga 
      acaaatatt tttagcaaa tctcaccaa gtcggttcg 
      tttgatccc aaagatctc ccgaaaacg ttacatttg 
      ttagatgat tccgaattg gaagactct tcaaggaac 
      ctacactta ctccatgcc gacttgcca tcgcccacc 
      atcgactta tcattgcaa gcatcacag aatacagcg 
      gaaaattcg atgcttctt acgaggttg gcagaggat 
      tctctgggt acagaggat caagtctgc acaatgcaa 
      ggttgtgtt cgccgaaag actgttctg aaagaagga 
      cgtaagcca actgtgact tcttggcaa cgttactgg 
      ttacaaatc tggactaca tcacttgtt tacttttca 
      ccgaaatct ttcaaaggt caccaacgg tctgacttc 
      aaaagagag ccatgtaag ctggtaccg ctaatgggc 
      tgtcaaata attctaggt gacaatagt gtccagcaa 
      gattgtttc caaatcatc gatcatcat cgagggaac 
      atctacaaa tacagggcg ggcactaaa agtttagcg 
      gaaaagtgg tgtcgacat ttgcaacaa gcagctaac 
      ggagaacaa actcctctt cctagtaat ctcatgtct 
      tttgagtaa
    • >TC002561 RB
      atgatgcgc tactccgaa ataccaagg gatatttca 
      ggagacagt ctaaccgca ctcaaaata ccggagagt 
      cgaaagcag gatagcttt aggacctcc gatatcgat 
      ggcgattcg gtttcactg tcttcgtct acgagccat 
      gacaggttt ccttcagaa attaggttt aggaaaata 
      tcaacaccg gctgtctcc agtggaccg ggaccaccc 
      tttacctac tataactac gggatatca ccggaagac 
      ttcgccagt caattgaca ttgctcgat ttacctgtg 
      ttcctcaac atccaaccc gacgaactc accagctgt 
      gcatggaat aagaaaaac aagttgacc atggcgccc 
      aatgttgtt gctttcacg agacgattc aatcatgtg 
      agcttctgg acggtgcaa gaaatactc agcggaccg 
      acacccaaa caaagagca gaaatactc gcgtttttt 
      gttcgaata gcgaagaag ttgtacgat ttgaataat 
      ttgcattcg ttatttgca atgatttca gccttacaa 
      agtgcctcg atttacaga ttatcaaag acgtggact 
      tgtttgtct aaaaaagat aaacagact tttgacaag 
      ctggccgag gtgttttcg gatgctgat aattggtcg 
      aatctcagg cgacatatt gaaagtttg aaacttcct 
      tgcataccc tatttaggt ttgtatctg actgatctt 
      gtttacatt gacatggcc catcctcat tcgggtggt 
      ttggaatcg cagcaaagg actctcaaa atgaacaat 
      attttacgc gtaatctcg aattatcag cattctgat 
      tattctcac ttgactgca atacctcac attcaagac 
      tatttgaac tcaatcagg tacatcgag gaacttcaa 
      aaattcgtc gaagatgat cagtacaaa ctttcattg 
      aaactggaa ccactttcg ccggcgccg agttcctca 
      agttgcagc tcaaaagaa tcggtgatt gccgatgca 
      gccgcaatc gcatcatta aatctctcg cctgcaaag 
      gcttccagc ggatcatta cgtcttcac gccgttaca 
      tcaggaagt aaattcata ccgggtcac agaaaatgc 
      cgcagttta ggaacaaat atttttagc aaatctcac 
      caagtcggt tcgtttgat cccaaagat ctcccgaaa 
      acgttacat ttgttagat gattccgaa ttggaagac 
      tcttcaagg aacctacac ttactccat gccgacttg 
      ccatcgccc accatcgac ttatcattg caagcatca 
      cagaataca gcggaaaat tcgatgctt cttacgagg 
      ttggcagag gattctctg ggtacagag gatcaagtc 
      tgcacaatg caaggttgt gttcgccga aagactgtt 
      ctgaaagaa ggacgtaag ccaactgtg acttcttgg 
      caacgttac tggttacaa atctggact acatcactt 
      gtttacttt tcaccgaaa tctttcaaa ggtcaccaa 
      cggtctgac ttcaaaaga gagccatgt aagctggta 
      ccgctaatg ggctgtcaa ataattcta ggtgacaat 
      agtgtccag caagattgt ttccaaatc atcgatcat 
      catcgaggg aacatctac aaatacagg gcgggcact 
      aaaagttta gcggaaaag tggtgtcga catttgcaa 
      caagcagct aacggagaa caaactcct cttcctagt 
      aatctcatg tcttttgag taa
    mRNA sequence
    • >TC002561 RA
      tatacctac cttctagca gtatttaac aaacaagtc 
      cataattta actagagag aaatataaa taatattag 
      cgcattatt gtataaatg ttagagttt atttttcaa 
      tgtatagta ctataccta acgttgaat tgttttaac 
      ttgtaaatt atttacgat aaatatcaa caaatatca 
      aaactagtt agacgatag cttagacgt gtagataaa 
      gcaccaacg tttgaacaa tctacaact gtagttaat 
      ttgaatccg tttacaaag tcccacctt gtaaactgc 
      acactattt atttattac aataaagtt ttattttaa 
      tacccgact tttgaattt cccacacca tgttcggtc 
      ccagtcggc cgcttccgt cccacttga catgcgtaa 
      acataacca aagtacacc tgtcactaa tgtgaatgt 
      cagtcgtat tttggtcgg ctttttgca agtgttgga 
      ggtgcacat atctcaaaa aatagttta gtttttaaa 
      taaatcact cagttttgt ccccatgtt ctagtttta 
      caaattatg tgtgtttgc cagcaaacc tccattcaa 
      acaacagcc caaaaccgc aatttaatt tagattttc 
      tacaagaac catgacacg aaaatgatg cgctactcc 
      gaaatacca agggatatt tcaggagac agtctaacc 
      gcactcaaa ataccggag agtcgaaag caggatagc 
      tttaggacc tccgatatc gatggcgat tcggtttca 
      ctgtcttcg tctacgagc catgacagg tttccttca 
      gaaattagg tttaggaaa atatcaaca ccggctgtc 
      tccagtgga ccgggacca ccctttacc tactataac 
      tacggttct ctcgcatct gtacctgcc catatcaaa 
      acccaaagc ttgccgagc aatgcccga accaaaaac 
      tacagctgt gctattttc gacatactc aggatatca 
      ccggaagac ttcgccagt caattgaca ttgctcgat 
      ttacctgtg ttcctcaac atccaaccc gacgaactc 
      accagctgt gcatggaat aagaaaaac aagttgacc 
      atggcgccc aatgttgtt gctttcacg agacgattc 
      aatcatgtg agcttctgg acggtgcaa gaaatactc 
      agcggaccg acacccaaa caaagagca gaaatactc 
      gcgtttttt gttcgaata gcgaagaag ttgtacgat 
      ttgaataat ttgcattcg ttatttgca atgatttca 
      gccttacaa agtgcctcg atttacaga ttatcaaag 
      acgtggact tgtttgtct aaaaaagat aaacagact 
      tttgacaag ctggccgag gtgttttcg gatgctgat 
      aattggtcg aatctcagg cgacatatt gaaagtttg 
      aaacttcct tgcataccc tatttaggt ttgtatctg 
      actgatctt gtttacatt gacatggcc catcctcat 
      tcgggtggt ttggaatcg cagcaaagg actctcaaa 
      atgaacaat attttacgc gtaatctcg aattatcag 
      cattctgat tattctcac ttgactgca atacctcac 
      attcaagac tatttgaac tcaatcagg tacatcgag 
      gaacttcaa aaattcgtc gaagatgat cagtacaaa 
      ctttcattg aaactggaa ccactttcg ccggcgccg 
      agttcctca agttgcagc tcaaaagaa tcggtgatt 
      gccgatgca gccgcaatc gcatcatta aatctctcg 
      cctgcaaag gcttccagc ggatcatta cgtcttcac 
      gccgttaca tcaggaagt aaattcata ccgggtcac 
      agaaaatgc cgcagttta ggaacaaat atttttagc 
      aaatctcac caagtcggt tcgtttgat cccaaagat 
      ctcccgaaa acgttacat ttgttagat gattccgaa 
      ttggaagac tcttcaagg aacctacac ttactccat 
      gccgacttg ccatcgccc accatcgac ttatcattg 
      caagcatca cagaataca gcggaaaat tcgatgctt 
      cttacgagg ttggcagag gattctctg ggtacagag 
      gatcaagtc tgcacaatg caaggttgt gttcgccga 
      aagactgtt ctgaaagaa ggacgtaag ccaactgtg 
      acttcttgg caacgttac tggttacaa atctggact 
      acatcactt gtttacttt tcaccgaaa tctttcaaa 
      ggtcaccaa cggtctgac ttcaaaaga gagccatgt 
      aagctggta ccgctaatg ggctgtcaa ataattcta 
      ggtgacaat agtgtccag caagattgt ttccaaatc 
      atcgatcat catcgaggg aacatctac aaatacagg 
      gcgggcact aaaagttta gcggaaaag tggtgtcga 
      catttgcaa caagcagct aacggagaa caaactcct 
      cttcctagt aatctcatg tcttttgag taatttgcg 
      tgactgaat aatactgtt gatatttat tttccatga 
      ttatttatt ttaagttgt accttcttt tttgtacat 
      ttgcattta ttacatat
    • >TC002561 RB
      tatacctac cttctagca gtatttaac aaacaagtc 
      cataattta actagagag aaatataaa taatattag 
      cgcattatt gtataaatg ttagagttt atttttcaa 
      tgtatagta ctataccta acgttgaat tgttttaac 
      ttgtaaatt atttacgat aaatatcaa caaatatca 
      aaactagtt agacgatag cttagacgt gtagataaa 
      gcaccaacg tttgaacaa tctacaact gtagttaat 
      ttgaatccg tttacaaag tcccacctt gtaaactgc 
      acactattt atttattac aataaagtt ttattttaa 
      tacccgact tttgaattt cccacacca tgttcggtc 
      ccagtcggc cgcttccgt cccacttga catgcgtaa 
      acataacca aagtacacc tgtcactaa tgtgaatgt 
      cagtcgtat tttggtcgg ctttttgca agtgttgga 
      ggtgcacat atctcaaaa aatagttta gtttttaaa 
      taaatcact cagttttgt ccccatgtt ctagtttta 
      caaattatg tgtgtttgc cagcaaacc tccattcaa 
      acaacagcc caaaaccgc aatttaatt tagattttc 
      tacaagaac catgacacg aaaatgatg cgctactcc 
      gaaatacca agggatatt tcaggagac agtctaacc 
      gcactcaaa ataccggag agtcgaaag caggatagc 
      tttaggacc tccgatatc gatggcgat tcggtttca 
      ctgtcttcg tctacgagc catgacagg tttccttca 
      gaaattagg tttaggaaa atatcaaca ccggctgtc 
      tccagtgga ccgggacca ccctttacc tactataac 
      tacgggata tcaccggaa gacttcgcc agtcaattg 
      acattgctc gatttacct gtgttcctc aacatccaa 
      cccgacgaa ctcaccagc tgtgcatgg aataagaaa 
      aacaagttg accatggcg cccaatgtt gttgctttc 
      acgagacga ttcaatcat gtgagcttc tggacggtg 
      caagaaata ctcagcgga ccgacaccc aaacaaaga 
      gcagaaata ctcgcgttt tttgttcga atagcgaag 
      aagttgtac gatttgaat aatttgcat tcgttattt 
      gcaatgatt tcagcctta caaagtgcc tcgatttac 
      agattatca aagacgtgg acttgtttg tctaaaaaa 
      gataaacag acttttgac aagctggcc gaggtgttt 
      tcggatgct gataattgg tcgaatctc aggcgacat 
      attgaaagt ttgaaactt ccttgcata ccctattta 
      ggtttgtat ctgactgat cttgtttac attgacatg 
      gcccatcct cattcgggt ggtttggaa tcgcagcaa 
      aggactctc aaaatgaac aatatttta cgcgtaatc 
      tcgaattat cagcattct gattattct cacttgact 
      gcaatacct cacattcaa gactatttg aactcaatc 
      aggtacatc gaggaactt caaaaattc gtcgaagat 
      gatcagtac aaactttca ttgaaactg gaaccactt 
      tcgccggcg ccgagttcc tcaagttgc agctcaaaa 
      gaatcggtg attgccgat gcagccgca atcgcatca 
      ttaaatctc tcgcctgca aaggcttcc agcggatca 
      ttacgtctt cacgccgtt acatcagga agtaaattc 
      ataccgggt cacagaaaa tgccgcagt ttaggaaca 
      aatattttt agcaaatct caccaagtc ggttcgttt 
      gatcccaaa gatctcccg aaaacgtta catttgtta 
      gatgattcc gaattggaa gactcttca aggaaccta 
      cacttactc catgccgac ttgccatcg cccaccatc 
      gacttatca ttgcaagca tcacagaat acagcggaa 
      aattcgatg cttcttacg aggttggca gaggattct 
      ctgggtaca gaggatcaa gtctgcaca atgcaaggt 
      tgtgttcgc cgaaagact gttctgaaa gaaggacgt 
      aagccaact gtgacttct tggcaacgt tactggtta 
      caaatctgg actacatca cttgtttac ttttcaccg 
      aaatctttc aaaggtcac caacggtct gacttcaaa 
      agagagcca tgtaagctg gtaccgcta atgggctgt 
      caaataatt ctaggtgac aatagtgtc cagcaagat 
      tgtttccaa atcatcgat catcatcga gggaacatc 
      tacaaatac agggcgggc actaaaagt ttagcggaa 
      aagtggtgt cgacatttg caacaagca gctaacgga 
      gaacaaact cctcttcct agtaatctc atgtctttt 
      gagtaattt gcgtgactg aataatact gttgatatt 
      tattttcca tgattattt attttaagt tgtaccttc 
      ttttttgta catttgcat ttattacat at
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