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Gene information
General information
TC002547 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23718666..23727028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002547
    iBeetle screen iB_00400

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002547 RA
      MAFQQIQRA LLRPSFRAV AKLQSCNAS SSPNGYNFA 
      LNDTQKEFQ ELARKFARE EIIPVAAEY DKTGKYPWD 
      IIKKAWEIG LMNGHIPQE IGGMGNSVF DGCLIAEEL 
      AYGCTGIMT ALEASGLGQ TPVLIAGNK EQQKKYLGR 
      LLEEPLVAA YCVTEPGAG SDVSGIKTR AEKKGDEWI 
      INGQKMWIT NGGVANWYF VLARTNPDP KCPAGKAFT 
      GFIVERETP GLTPGRKEL NMGQRASDT RGITFEDVR 
      VPKENVLVG EGAGFKIAM GTFDKTRPP VAAGAVGLA 
      QRCLDEALK YANERKTFG VPIAHHQAV AFMLADMAV 
      GVETSRLAW MKSAWRVDQ GQRNSYEAA IAKCYAADV 
      ANKCATDAV QIFGGNGFN SEYPVEKLM RDAKIYQIY 
      EGTSQIQRI IISRSILDN AKQMS
    CDS sequence
    • >TC002547 RA
      atggctttt caacagatt caaagagct ctcctccga 
      ccatcgttc agagcagtt gctaaattg caaagttgc 
      aatgcttcg tcatctcca aacggatac aactttgct 
      ttgaatgac actcagaaa gagtttcaa gaactggct 
      agaaaattt gccagagag gagattatt cctgttgct 
      gctgaatat gacaaaact gggaaatat ccatgggat 
      attattaaa aaagcgtgg gagattggg ctcatgaat 
      ggacatatc ccccaagaa attggtggt atgggaaat 
      agtgttttc gatggttgt ctcattgca gaagagttg 
      gcttatggg tgcactggt attatgact gctttggaa 
      gccagtggc ttggggcaa acaccagtc ctgattgcg 
      ggaaataaa gaacagcaa aagaaatat ttgggccgt 
      ttgcttgaa gaacctctt gttgctgcc tattgtgtc 
      actgaacca ggtgcgggt tcggatgtg agtggcatt 
      aaaacacga gctgagaaa aaaggtgat gagtggatt 
      attaatggt cagaaaatg tggattaca aatggaggc 
      gttgccaat tggtacttc gtgctcgca cggaccaat 
      ccagatcca aaatgtcca gctggtaaa gcattcact 
      ggatttatt gttgagagg gaaactcct ggattgacc 
      ccgggaagg aaggaactg aacatgggt caaagggct 
      tccgacact cgcggcatc accttcgaa gacgtccgc 
      gtccccaaa gaaaacgtc cttgtcggc gaaggcgcc 
      ggtttcaag atcgccatg ggcacattc gacaaaacc 
      cgcccccct gtggccgca ggagccgtc ggcttggcc 
      cagagatgt ctggatgag gccctcaaa tacgccaac 
      gagcgcaaa accttcggc gttccgatc gctcaccac 
      caagccgtt gccttcatg ctggccgat atggcagtc 
      ggagtcgaa acctcccga ctcgcctgg atgaagtcg 
      gcctggagg gtggaccaa ggccagagg aacagctac 
      gaagcggca attgccaag tgttatgct gctgatgtt 
      gccaacaag tgtgccact gatgcggtg caaattttc 
      gggggtaat gggttcaac agcgaatat cccgtcgaa 
      aaactcatg agggatgcc aagatttat caaatttat 
      gaagggacc tcgcagatc cagaggatt attatttcg 
      aggtctatt ctcgataat gccaaacag atgagctag
    mRNA sequence
    • >TC002547 RA
      cagatttgt gtataactg gttgcctgc taatcgtga 
      cgttgtaca tttattttt atcagctgt tcactttaa 
      ttttttcga tttatttat atttgcgta cgataagac 
      tttgatttg aacctgtta gtatcatta tcaatattg 
      tttagtcga tttttacac gggacggtt caaaataca 
      aggttaatc agccaaggt gcaaaggtt catacaatt 
      cgtttattc tcaatctcg gcagtttaa ctgggacac 
      atatcacgc aaagatggc ttttcaaca gattcaaag 
      agctctcct ccgaccatc gttcagagc agttgctaa 
      attgcaaag ttgcaatgc ttcgtcatc tccaaacgg 
      atacaactt tgctttgaa tgacactca gaaagagtt 
      tcaagaact ggctagaaa atttgccag agaggagat 
      tattcctgt tgctgctga atatgacaa aactgggaa 
      atatccatg ggatattat taaaaaagc gtgggagat 
      tgggctcat gaatggaca tatccccca agaaattgg 
      tggtatggg aaatagtgt tttcgatgg ttgtctcat 
      tgcagaaga gttggctta tgggtgcac tggtattat 
      gactgcttt ggaagccag tggcttggg gcaaacacc 
      agtcctgat tgcgggaaa taaagaaca gcaaaagaa 
      atatttggg ccgtttgct tgaagaacc tcttgttgc 
      tgcctattg tgtcactga accaggtgc gggttcgga 
      tgtgagtgg cattaaaac acgagctga gaaaaaagg 
      tgatgagtg gattattaa tggtcagaa aatgtggat 
      tacaaatgg aggcgttgc caattggta cttcgtgct 
      cgcacggac caatccaga tccaaaatg tccagctgg 
      taaagcatt cactggatt tattgttga gagggaaac 
      tcctggatt gaccccggg aaggaagga actgaacat 
      gggtcaaag ggcttccga cactcgcgg catcacctt 
      cgaagacgt ccgcgtccc caaagaaaa cgtccttgt 
      cggcgaagg cgccggttt caagatcgc catgggcac 
      attcgacaa aacccgccc ccctgtggc cgcaggagc 
      cgtcggctt ggcccagag atgtctgga tgaggccct 
      caaatacgc caacgagcg caaaacctt cggcgttcc 
      gatcgctca ccaccaagc cgttgcctt catgctggc 
      cgatatggc agtcggagt cgaaacctc ccgactcgc 
      ctggatgaa gtcggcctg gagggtgga ccaaggcca 
      gaggaacag ctacgaagc ggcaattgc caagtgtta 
      tgctgctga tgttgccaa caagtgtgc cactgatgc 
      ggtgcaaat tttcggggg taatgggtt caacagcga 
      atatcccgt cgaaaaact catgaggga tgccaagat 
      ttatcaaat ttatgaagg gacctcgca gatccagag 
      gattattat ttcgaggtc tattctcga taatgccaa 
      acagatgag ctaggtttg aagcgttga tatttcatc 
      atttattgt aattttaag ataaattta ttataacgt 
      attttacga gattatttt gtataaaac tgaattttc 
      aatatatgc tttacaaat ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY