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Gene information
General information
TC002529 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 269953..273739
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002529
    iBeetle screen iB_00397
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002529 RA
      MTTATDKGS VLCYKGSNF FRQRLVLSI LSGKPVRIT 
      HIRVLEDEP GLREFEVNL IRLLDKITN GSVIELNET 
      GTAVYFQPG LLYGGTVQH ECSTQRGVG YYLEALVML 
      GLFCKQPLN ATLQGVTNN NIDPSVDLI RTSALSTLK 
      KFFLDDEGL DVKISKRGL LPLGGGEVI FKCPVRKHL 
      RPIHSLDSG MVKRIRGTA FALRVSPAI ANRIVDKAK 
      GVLLNFLPD IYINTDQCR GKQSGKSPG FGIHLTAET 
      TNGVIYSSE QVSNVVSNG EDPSVPEDV GVAAAQRLL 
      HEIYLGGAT DSTCQSVAI LCMALGQQD VSKFVTGPL 
      TENAIGFLR HLKEFFGVT FKLEHYEPD EESPGTGSQ 
      KVMLTCVGL GYTNISKRT I
    CDS sequence
    • >TC002529 RA
      atgaccact gccactgat aagggaagt gttttgtgt 
      tacaagggc agcaatttt tttcgccag aggttggtt 
      ttatcgatt ttgagcggt aaaccggtc cgcataacc 
      cacattcga gtgttggag gatgagcca ggtttaaga 
      gaatttgaa gtgaattta attcgtctt ctggataaa 
      attacaaac ggttctgtt attgaatta aatgaaaca 
      gggacagcg gtgtatttc caacctggg ttactgtac 
      ggaggtacg gtacaacat gagtgttca actcagaga 
      ggggttggt tattacttg gaagctttg gttatgctt 
      ggtttattt tgtaagcaa cccttaaat gctactcta 
      caaggcgtc acaaataat aatattgat ccctccgtt 
      gatttaata aggacgtct gcgttaagt acgttgaaa 
      aagtttttc ttggatgat gagggactt gatgtgaag 
      atttccaag agaggcttg cttccattg ggtggcggt 
      gaagtaatt ttcaaatgc cccgtccgt aagcacctc 
      agaccaatc cattccctc gacagtgga atggtaaaa 
      cgcatcaga ggcacagca ttcgcatta cgcgtctct 
      cctgccatt gctaaccga atagttgac aaagctaaa 
      ggcgtcctc ctcaacttc ctccccgac atttacatc 
      aacaccgac caatgtcga ggaaaacaa tcgggcaaa 
      agccccggc tttggcatt cacctaacg gcagaaacc 
      accaacggt gtcatttat tcatcagaa caagtctca 
      aatgttgtt tcaaacggt gaagatcct tcagttcct 
      gaagatgta ggcgttgct gctgcgcaa agattactg 
      catgaaatt tatctaggt ggcgccaca gactcgacc 
      tgccaatcg gtggccatc ttgtgcatg gcattggga 
      caacaagat gtctccaaa tttgtaact gggcctttg 
      accgaaaat gcgatcggt tttttaaga catttgaag 
      gaatttttc ggggttact ttcaaacta gagcactat 
      gaacctgat gaggagagt ccagggaca gggagccag 
      aaagttatg ttaacttgt gttggttta ggttacacg 
      aatattagt aaacggacg atttaa
    mRNA sequence
    • >TC002529 RA
      ttcacgact actaagaac acgtagaaa aatatttag 
      gaacccaaa tgtgtttcg ataattcat tcgtgtgaa 
      accacctct gtgtgagct gtagctagt agttcaaaa 
      atcttgcac gtgtgtgac agcataacc ttcaaaaat 
      tttgaattc aatttccaa aaatgacca ctgccactg 
      ataagggaa gtgttttgt gttacaagg gcagcaatt 
      tttttcgcc agaggttgg ttttatcga ttttgagcg 
      gtaaaccgg tccgcataa cccacattc gagtgttgg 
      aggatgagc caggtttaa gagaatttg aagtgaatt 
      taattcgtc ttctggata aaattacaa acggttctg 
      ttattgaat taaatgaaa cagggacag cggtgtatt 
      tccaacctg ggttactgt acggaggta cggtacaac 
      atgagtgtt caactcaga gaggggttg gttattact 
      tggaagctt tggttatgc ttggtttat tttgtaagc 
      aacccttaa atgctactc tacaaggcg tcacaaata 
      ataatattg atccctccg ttgatttaa taaggacgt 
      ctgcgttaa gtacgttga aaaagtttt tcttggatg 
      atgagggac ttgatgtga agatttcca agagaggct 
      tgcttccat tgggtggcg gtgaagtaa ttttcaaat 
      gccccgtcc gtaagcacc tcagaccaa tccattccc 
      tcgacagtg gaatggtaa aacgcatca gaggcacag 
      cattcgcat tacgcgtct ctcctgcca ttgctaacc 
      gaatagttg acaaagcta aaggcgtcc tcctcaact 
      tcctccccg acatttaca tcaacaccg accaatgtc 
      gaggaaaac aatcgggca aaagccccg gctttggca 
      ttcacctaa cggcagaaa ccaccaacg gtgtcattt 
      attcatcag aacaagtct caaatgttg tttcaaacg 
      gtgaagatc cttcagttc ctgaagatg taggcgttg 
      ctgctgcgc aaagattac tgcatgaaa tttatctag 
      gtggcgcca cagactcga cctgccaat cggtggcca 
      tcttgtgca tggcattgg gacaacaag atgtctcca 
      aatttgtaa ctgggcctt tgaccgaaa atgcgatcg 
      gttttttaa gacatttga aggaatttt tcggggtta 
      ctttcaaac tagagcact atgaacctg atgaggaga 
      gtccaggga cagggagcc agaaagtta tgttaactt 
      gtgttggtt taggttaca cgaatatta gtaaacgga 
      cgatttaat ttacttaaa ctatacatt ttattttaa 
      ttaaattta caaacgttg tttcaattt atttccaat 
      cagagtggc ctacttgtc aataagtca cggccactt 
      tgattcgaa attaaatta aatatatgg tacgtaa