Status
Please wait ...
Gene information
General information
TC002527 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 293277..300329
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002527
    iBeetle screen iB_00396
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002527 RA
      MALFFNIIV IALCCGSCL GRIHKLDIT NDARKYIAL 
      STFGFYQGG VLDVQLKNF RASPENDSA VFGFSLDRT 
      INDAMNPYL GSHQEHCIL QEPVNTASD VAIIYFILD 
      LKSKKLKIK CEDQQLVHI YKNKNEVPS VLSAHRQKR 
      QDVSDHCSN LDFDITAEV IRGRTYYNA SFTLYVASV 
      NEEGLYNLY FHSCPNYQP GTEVSVDFT IEIVEQNMS 
      SFLSAGEMP LPALYSMMA ILFLLSGMF WVFLLRQSR 
      HIVFKIHYM MAVLVFLKA ISLAFHGVN YHYIERLGV 
      HLATWAVLF YVAHLLKGA LLFIVLVLI GTGWTFIKH 
      VLAPRDKRL FMAVIPLQV LANVAEIIL EESEEGARE 
      HVAWRNVFI LVDLLCCGC ILFPVVWSI RHLQEASQT 
      DGKAAMNLR KLKLFKHFY LMIVCYIYS TRMIVYLLK 
      ITVPFQYGW LDEMFREMA TYVFFVLTA YKFRPAAHN 
      PYFALSDDE DDDEMDKVL TESGATEGL SRVNSRVTK 
      VPLHSTLTE VTEEEKDAL LGQPESSHE YD
    CDS sequence
    • >TC002527 RA
      atggcactg ttttttaac attattgta atagcattg 
      tgttgtggc tcgtgtttg gggcgaata cacaaatta 
      gacatcaca aatgatgca cgaaagtac attgcgtta 
      agcacattt gggttttac caaggcggg gtcctggat 
      gttcagctg aaaaatttc agagcttcg cctgaaaat 
      gattcagca gtgtttgga tttagttta gaccgtaca 
      ataaacgat gcaatgaac ccgtaccta ggtagtcac 
      caagaacat tgtattttg caagagcct gttaacact 
      gcgagcgac gtggccata atttacttt atcctcgat 
      cttaaaagc aaaaaactt aaaattaaa tgtgaggac 
      caacaactt gtacatatt tacaaaaat aagaatgag 
      gttccctct gtgttatca gcacatcga caaaaacgg 
      caagacgtg tctgaccac tgcagtaac ttagacttt 
      gacataact gcagaagtt attagagga cggacttac 
      tacaatgct agtttcact ctgtacgtt gcatcagtg 
      aacgaagaa ggactgtac aacttatac ttccacagc 
      tgtccgaac taccaaccc ggaaccgaa gtctctgtc 
      gacttcaca atcgaaata gtcgagcaa aacatgagc 
      agctttctg tccgctggc gaaatgcca ctcccagcc 
      ttatactca atgatggca attttgttc ctcctctcc 
      ggcatgttt tgggtgttt ttgttgcgc caatcccgt 
      cacattgtc tttaaaatc cactacatg atggcagtc 
      cttgttttc ctcaaagca atttcccta gctttccac 
      ggggttaac taccactac attgaacgt ctcggtgtg 
      catttggcc acctgggct gtacttttc tacgtggcg 
      catttgtta aaaggcgcg ctacttttc atagttttg 
      gtgttaatt ggcacaggt tggactttt atcaaacat 
      gtgctggct ccgcgtgat aagcggctt tttatggcc 
      gttatcccg ctccaggtt ttggcaaat gtggctgaa 
      attatcctg gaggagagt gaggaaggg gctcgggaa 
      cacgtcgcc tggaggaat gtttttatt ttggtggat 
      ttgttgtgc tgtggttgt attttattt ccggtggtt 
      tggtcgatt cgtcatttg caagaagcg tctcagact 
      gatgggaaa gcagcaatg aatttaagg aagttgaaa 
      ctgttcaaa catttttat ttgatgatc gtttgttac 
      atttattcc actcggatg attgtctat ctgcttaaa 
      ataacagtg ccgtttcaa tacggctgg ttggatgaa 
      atgtttagg gaaatggca acgtatgtc ttttttgtc 
      ctaactgct tacaagttc cgaccagcg gctcataat 
      ccatatttt gcgctgagt gacgacgaa gatgacgat 
      gaaatggac aaagtactt actgagtca ggagcgact 
      gaaggctta agtagagtt aattcccgc gttaccaaa 
      gtgccacta cactccact ttgaccgaa gtgacagaa 
      gaagaaaaa gacgcattg ttgggacaa ccagaaagt 
      agccacgag tacgattaa
    mRNA sequence
    • >TC002527 RA
      tgtcaaatt aattacagc tgtaaaaag ttcatagtt 
      gagtgatat ttttattta gatgtgttt ttgtggaaa 
      ttatttact aaatctgtt tatgtagtt cagtgaatc 
      agactatga ccggtttta taacattta cacctttac 
      acgtatgcg tataagaaa aaattggca tcttagaca 
      tcacaagtt gatatgttt taacaccaa aatcgtaca 
      aaatggcac tgtttttta acattattg taatagcat 
      tgtgttgtg gctcgtgtt tggggcgaa tacacaaat 
      tagacatca caaatgatg cacgaaagt acattgcgt 
      taagcacat ttgggtttt accaaggcg gggtcctgg 
      atgttcagc tgaaaaatt tcagagctt cgcctgaaa 
      atgattcag cagtgtttg gatttagtt tagaccgta 
      caataaacg atgcaatga acccgtacc taggtagtc 
      accaagaac attgtattt tgcaagagc ctgttaaca 
      ctgcgagcg acgtggcca taatttact ttatcctcg 
      atcttaaaa gcaaaaaac ttaaaatta aatgtgagg 
      accaacaac ttgtacata tttacaaaa ataagaatg 
      aggttccct ctgtgttat cagcacatc gacaaaaac 
      ggcaagacg tgtctgacc actgcagta acttagact 
      ttgacataa ctgcagaag ttattagag gacggactt 
      actacaatg ctagtttca ctctgtacg ttgcatcag 
      tgaacgaag aaggactgt acaacttat acttccaca 
      gctgtccga actaccaac ccggaaccg aagtctctg 
      tcgacttca caatcgaaa tagtcgagc aaaacatga 
      gcagctttc tgtccgctg gcgaaatgc cactcccag 
      ccttatact caatgatgg caattttgt tcctcctct 
      ccggcatgt tttgggtgt ttttgttgc gccaatccc 
      gtcacattg tctttaaaa tccactaca tgatggcag 
      tccttgttt tcctcaaag caatttccc tagctttcc 
      acggggtta actaccact acattgaac gtctcggtg 
      tgcatttgg ccacctggg ctgtacttt tctacgtgg 
      cgcatttgt taaaaggcg cgctacttt tcatagttt 
      tggtgttaa ttggcacag gttggactt ttatcaaac 
      atgtgctgg ctccgcgtg ataagcggc tttttatgg 
      ccgttatcc cgctccagg ttttggcaa atgtggctg 
      aaattatcc tggaggaga gtgaggaag gggctcggg 
      aacacgtcg cctggagga atgttttta ttttggtgg 
      atttgttgt gctgtggtt gtattttat ttccggtgg 
      tttggtcga ttcgtcatt tgcaagaag cgtctcaga 
      ctgatggga aagcagcaa tgaatttaa ggaagttga 
      aactgttca aacattttt atttgatga tcgtttgtt 
      acatttatt ccactcgga tgattgtct atctgctta 
      aaataacag tgccgtttc aatacggct ggttggatg 
      aaatgttta gggaaatgg caacgtatg tcttttttg 
      tcctaactg cttacaagt tccgaccag cggctcata 
      atccatatt ttgcgctga gtgacgacg aagatgacg 
      atgaaatgg acaaagtac ttactgagt caggagcga 
      ctgaaggct taagtagag ttaattccc gcgttacca 
      aagtgccac tacactcca ctttgaccg aagtgacag 
      aagaagaaa aagacgcat tgttgggac aaccagaaa 
      gtagccacg agtacgatt aagagggat tacaagagc 
      ttgattaat tttctttgt cctactgtg atagtgttt 
      cagcttgtc atttttacg cacttcatt agatgctgg 
      agccaaatg atgcatttc ttatttatt cctctatta 
      tcctagatg taactaact taataaatt tgaaaattg 
      ttataagtt ccgtgtttt tgcttcaac cttgatcaa 
      atcagttat tagaatgtg gacgaccct gagggagca 
      caaagaccg aaattttaa taatatatt ttttagagc 
      gat