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Gene information
General information
TC002522 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 497779..533536
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002522
    iBeetle screen iB_00394
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002522 RA
      MGNNCCCCG SRRSQPDRL VYPGGIKTA HGLDGGPPR 
      LTINNPYTP DPQRSHVDI IRSRPTPTR PPLSVKGRV 
      VVALYNYEA RDSSDVSFK KGDRMEVID DSEGDWLNV 
      IHLITKVQG YIPGNFVAA ELSVESEDW FFENVSRKE 
      AEKLLLAEE NPRGTFLVR PSEHNPNGY SLSVKDWEE 
      IREYHVKHY KIKPLDNGG FYISTNKTF LTLVELVNA 
      YTKNAYGLC HVLSKPCPK PQPTMWDLG PTYRDKWEI 
      PREEIQLIQ RLGRGNFGE VWYGKWKNN IEVAVKMLR 
      EGTMSTQAF LQEAAIMKK FRHKRLVTL FGVCSEHEP 
      IYIVQEYMS KGSLLDFLR KDEGKELEF EDLVYIAFQ 
      VASGMEYLE SKQLIHRDL AARNVLIGE NNIAKICDF 
      GLARVIEDN EYCPKQGSR FPVKWTAPE AIVYGKFSI 
      KSDVWSYGI FLMELFTYG QVPYPGMHG REVIDMVEK 
      GYRMGKPTS HVLPDEIYS IMLQCWDAK PENRPTFEF 
      LTHYFEGFN VTSEVPYRE VPD
    CDS sequence
    • >TC002522 RA
      atgggcaat aattgctgc tgttgcggc agccgacgc 
      tcgcagccc gacagactt gtctatccg ggcggaatc 
      aaaacggca cacggactg gacggtggt ccacctcga 
      ctgacaatc aacaatccc tacacaccg gaccctcag 
      cggagtcat gtcgatata ataaggagt cggccgacc 
      cccacacgg ccccctctt tccgtaaag ggccgcgtg 
      gtcgtggcc ctttacaac tacgaggcc cgcgacagc 
      agcgatgtc tcgtttaag aaaggcgat cgcatggaa 
      gtcatcgat gactccgaa ggcgattgg ctaaatgtg 
      atacatctc ataaccaaa gtccagggc tacatccct 
      ggaaatttt gtagcggcc gaattgagc gtcgaaagc 
      gaagactgg ttttttgaa aacgtatcg cgaaaggaa 
      gccgagaag cttctcctc gccgaggaa aatccaaga 
      ggaactttc ctagtccgt ccttccgag cataatccc 
      aacgggtat tcgctctca gtcaaagac tgggaggag 
      atcagggaa taccacgtc aaacactac aaaatcaag 
      ccgcttgat aacggcgga ttctacatt tccaccaac 
      aaaacattc ctcacttta gtcgaatta gtcaatgct 
      tacaccaaa aacgcttat ggcctttgc cacgtcttg 
      tcgaagccg tgccccaag ccccagccc acgatgtgg 
      gacctgggg cccacctac cgcgacaaa tgggaaatc 
      ccacgcgag gaaatccaa ctcatccag cgcctaggc 
      cgcggcaac ttcggcgaa gtgtggtac ggcaaatgg 
      aaaaacaac atcgaagtc gccgttaaa atgctccgc 
      gagggcaca atgtccaca caagccttt ttacaagaa 
      gcggccatc atgaagaag ttccgccac aagcgcttg 
      gtcaccctc ttcggcgtg tgcagcgaa cacgaaccc 
      atctacatc gtccaggag tacatgtcc aagggcagc 
      ctcctcgac ttcctgcga aaggacgaa ggcaaggag 
      ctggagttc gaggacctg gtgtacata gcgttccaa 
      gtggccagc gggatggag tatctcgag tccaaacaa 
      ttgatccat cgggattta gccgcgcgt aatgtttta 
      atcggcgaa aataacatt gcgaaaatc tgcgatttc 
      gggcttgcg agggtgatt gaagacaat gagtattgc 
      cccaagcag ggctcgagg ttccccgtc aagtggacg 
      gcccccgag gccatcgtc tacgggaag ttctccata 
      aagtcggac gtctggtct tacggcatc ttcctgatg 
      gagctcttc acctacgga caggtcccg tacccgggg 
      atgcacggg cgcgaggtc atagacatg gtcgagaag 
      ggctacagg atgggcaaa cccacctcg cacgtgctc 
      cccgacgag atctactcg ataatgctg cagtgctgg 
      gacgcgaag cccgaaaac aggcccacc tttgagttt 
      ctgacccac tactttgaa gggttcaac gtgacgtca 
      gaggtgccc tatcgggag gtgcccgat tag
    mRNA sequence
    • >TC002522 RA
      tgtacgcag gttttgtgt tgatgttct tcaattaat 
      ttaagttta aaagtgttg tgatgtatg atcgcgtcc 
      caatttagt gaaaaaata cgtttttag tgatttgag 
      taaacttga acaacaatc atttggttg gactttgtt 
      atagttact ttataaata aggatgaac cggctgctg 
      gataccctg tgctgacct cttggtttc ggccgccgc 
      cgacaccgc catgggcaa taattgctg ctgttgcgg 
      cagccgacg ctcgcagcc cgacagact tgtctatcc 
      gggcggaat caaaacggc acacggact ggacggtgg 
      tccacctcg actgacaat caacaatcc ctacacacc 
      ggaccctca gcggagtca tgtcgatat aataaggag 
      tcggccgac ccccacacg gccccctct ttccgtaaa 
      gggccgcgt ggtcgtggc cctttacaa ctacgaggc 
      ccgcgacag cagcgatgt ctcgtttaa gaaaggcga 
      tcgcatgga agtcatcga tgactccga aggcgattg 
      gctaaatgt gatacatct cataaccaa agtccaggg 
      ctacatccc tggaaattt tgtagcggc cgaattgag 
      cgtcgaaag cgaagactg gttttttga aaacgtatc 
      gcgaaagga agccgagaa gcttctcct cgccgagga 
      aaatccaag aggaacttt cctagtccg tccttccga 
      gcataatcc caacgggta ttcgctctc agtcaaaga 
      ctgggagga gatcaggga ataccacgt caaacacta 
      caaaatcaa gccgcttga taacggcgg attctacat 
      ttccaccaa caaaacatt cctcacttt agtcgaatt 
      agtcaatgc ttacaccaa aaacgctta tggcctttg 
      ccacgtctt gtcgaagcc gtgccccaa gccccagcc 
      cacgatgtg ggacctggg gcccaccta ccgcgacaa 
      atgggaaat cccacgcga ggaaatcca actcatcca 
      gcgcctagg ccgcggcaa cttcggcga agtgtggta 
      cggcaaatg gaaaaacaa catcgaagt cgccgttaa 
      aatgctccg cgagggcac aatgtccac acaagcctt 
      tttacaaga agcggccat catgaagaa gttccgcca 
      caagcgctt ggtcaccct cttcggcgt gtgcagcga 
      acacgaacc catctacat cgtccagga gtacatgtc 
      caagggcag cctcctcga cttcctgcg aaaggacga 
      aggcaagga gctggagtt cgaggacct ggtgtacat 
      agcgttcca agtggccag cgggatgga gtatctcga 
      gtccaaaca attgatcca tcgggattt agccgcgcg 
      taatgtttt aatcggcga aaataacat tgcgaaaat 
      ctgcgattt cgggcttgc gagggtgat tgaagacaa 
      tgagtattg ccccaagca gggctcgag gttccccgt 
      caagtggac ggcccccga ggccatcgt ctacgggaa 
      gttctccat aaagtcgga cgtctggtc ttacggcat 
      cttcctgat ggagctctt cacctacgg acaggtccc 
      gtacccggg gatgcacgg gcgcgaggt catagacat 
      ggtcgagaa gggctacag gatgggcaa acccacctc 
      gcacgtgct ccccgacga gatctactc gataatgct 
      gcagtgctg ggacgcgaa gcccgaaaa caggcccac 
      ctttgagtt tctgaccca ctactttga agggttcaa 
      cgtgacgtc agaggtgcc ctatcggga ggtgcccga 
      ttagtgggg taccaccaa tacgtataa acactagat 
      ctcagttgt gtttggttt tccactcgc ccgtttgct 
      tgccgcaca ttcttcaaa atttctaag actggtttg 
      agttagtgt ccgaagcca cgtacgata tcacgcagt 
      acaaaaagg caaaattca agtgaccta ttcgagaat 
      ttaactttt atacgactt tttgtacgt ctttttact 
      caaaatatt ggacgtgga ccaatactt gcttccctt 
      ccttcttca gtgtattat tgtatgtgt ggttgtctt 
      cagttcgag taatatcgc gtgtggtgg acacggagc 
      tctctattt ttgacggag gccacacgt ggtattggc 
      tttttgatt tttggtttt gtgcaataa acaatgtac 
      aaaattcat tctaaatta ttgagaaac ttattttta 
      gagttatat aagtgttaa tcacgtctt aaatgtatt 
      gcttttatt tttgtttta agtatattt ttattttat 
      gaggaaaca tagtcatga tagtgatcg tgagtgtat 
      tagtataat tatttatgt ttgagatgg ttatacagt 
      cagatttga tccagtcaa gtttttatc tgttcttaa 
      gaaaagcca tatgttatt gatttttat ataggaaca 
      atcatatta aattttgta catatacag tatgaataa 
      aacattgtg ctactcagc gacagagaa cgcgcaaaa 
      agatgcgca ccaacgcag taatggaaa gtacaacag 
      tatatgaac ctttgtgaa tattgccgt gtcctgtta 
      acagaagga aattcggga actcaaatg tttaaaaag 
      aaattgttc aaatgaaaa attgaatta ttaaggaag 
      ataatgaat tgataaatt gataacaca aaatggttt 
      ctttggatg ctgcctcaa gttaaactc gggggactg 
      ataactctg gaaaagatg tgcagattt ttctgattt 
      catcaaaat tgtttgttg cctttcgga tttatggtg 
      ctgtcctgg aattattaa aattctttc tggaaccat 
      ttggaatgg aattatctg gattagtgt ttttctttt 
      tttataaac tgtatttca ctatttctc aaaacgatt 
      tcttatttc cgtggtagt tttaagaaa tagtgaaat 
      acgataatt gtcaaaagg gaaaatcag cataatttg 
      gatttacag gaattattt tgaaattag agaagttaa 
      tttggattt tggaattac tgttgagta ttttggagg 
      atttttttg taacgga