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Gene information
General information
TC002520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 543758..547706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002520
    iBeetle screen iB_00393
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002520 RA
      MATSTTTTI IQTVSRLSK VSFSWYDYI LFSVMLVLS 
      ALIGIYFGC FGSKQSSAN EYLMGGKTM RVIPIAISL 
      VASHTSGIT LLALPADIY RYGAAYWLG GISMAILSV 
      VTIYVYLPV FYNLQIIST YEYLERRFD KKTRSFASF 
      LYAVSVFLY LPIVVYIPA LAFSAASGI DVHLIAPVV 
      CAVCIFYTT IGGLKAVVW TDTLQFSVT VGAIITVFI 
      LGVKSAGGF LQVWNKAVE GQRLDIFDF DLDPTKRDS 
      FWAIVIGLT VHWMGHTSV NQGCVQKFL AVSTLKKSS 
      FVLTRFIMF AKYSDCDPF TTKQVTRND QLLPYYVMD 
      VARNIPGLS GLFIAGVFS AGLSTLSAT LNCLAGTLY 
      EDFISKFMH KGITEKSAS NILKIIVIV TGVACTVLV 
      FVIEHMGGL LKLSISLGG VTNGPLLGL FTLGMLFPR 
      ANSKGAFYG TIGGVIFIS TLIFPTKYF ETQGLIKYP 
      PKPLSTERC DFFNETNIF ENVLHRQVC TFNVLKIIY 
      SSSYRNSST YFEPPAIFR ISFYYYTLI GALSTIIFA 
      LIISYMTNK NDPPVDKSL ISPVSHFLL PKDTKTNGV 
      VEYNTVERA LEVVTTNSG KENK
    CDS sequence
    • >TC002520 RA
      atggcaact tccacaacc acaacaata atccagaca 
      gtttccaga ctgtcgaaa gttagtttt tcctggtat 
      gactacatc ctgttttcg gtcatgcta gtccttagt 
      gctttgatt ggtatttac tttggctgc tttggaagt 
      aaacaatca agtgccaac gagtatctc atgggcgga 
      aaaacaatg agggtcata ccaatcgct atatctcta 
      gtcgcaagt cacacttcc gggattact ctccttgca 
      cttccggcc gatatttac cggtatggg gccgcctat 
      tggctgggg ggcatctcc atggccatt ctttccgta 
      gtgacaatc tacgtctac ttaccggtg ttctacaat 
      ttacaaatt attagcacg tacgagtat ttggaacga 
      cgttttgat aagaagacg cgatcgttt gcgtcgttt 
      ctttacgca gtttcggtc tttttgtat ttgccaatc 
      gttgtctat attccagcc ttggcgttt tcggccgct 
      tcagggatt gacgtccac ttgatagct cctgttgtt 
      tgcgcagtt tgtattttt tacactacc atagggggg 
      cttaaagca gtcgtctgg actgacact ctccagttt 
      agtgtaaca gtgggggcc atcatcaca gtctttatc 
      ttgggcgtg aaatcagca ggtggtttc ttgcaagtg 
      tggaacaag gcagttgaa ggccaaaga ttggatatt 
      ttcgacttc gatttggac ccaacaaag cgtgacagt 
      ttctgggcc attgtgatc ggtttaacc gtccactgg 
      atggggcac accagtgtt aaccaaggc tgtgtccag 
      aaatttctc gcagtttcg actttgaaa aaatccagt 
      tttgttttg actaggttt atcatgttt gccaaatac 
      tctgactgt gatcctttc acaacgaaa caagtgaca 
      cgaaacgac cagttgctc ccttactac gtgatggac 
      gttgcccgg aacattccg gggctttcc ggtcttttt 
      attgccgga gttttcagt gcgggtttg agcacactt 
      tcagcaact ttgaactgt ttggctgga actttatac 
      gaggacttt atcagcaag tttatgcat aaagggatt 
      acggaaaaa tccgccagc aatattttg aaaataatt 
      gtgatagtc accggagtt gcgtgcacg gttctggtg 
      tttgtgatt gaacacatg ggagggtta ttaaaattg 
      tcgataagt ctaggaggg gttacaaac ggccctctg 
      ttgggtctg tttactttg ggaatgctt tttccgaga 
      gccaactcc aaaggggcg ttttatggc acaatcggt 
      ggtgtaatt ttcatttca acgttaatt ttcccaact 
      aaatatttt gagacgcaa ggattgatt aaatatcct 
      cctaaaccg ttgtcaacc gaacggtgc gattttttt 
      aacgagacg aatattttt gaaaatgtt ttacacagg 
      caagtttgc acgtttaac gttttgaaa ataatttat 
      tcaagttct tacaggaat agctcaact tattttgaa 
      cctcctgca attttccga atctcgttt tattattac 
      acgcttata ggggctctt tctactata atttttgct 
      ttaataatt agctacatg acaaataaa aatgaccct 
      ccggttgat aaatcgctg ataagtcca gtgagccac 
      tttttgctt cccaaagac actaaaacc aatggtgtt 
      gttgagtac aatactgtt gagagggcc ctcgaagtt 
      gttacaaca aactcagga aaagagaac aaatga
    mRNA sequence
    • >TC002520 RA
      atatcaaga tttttaaat ttcgggaaa agaaattag 
      aatgtaaga atggcaact tccacaacc acaacaata 
      atccagaca gtttccaga ctgtcgaaa gttagtttt 
      tcctggtat gactacatc ctgttttcg gtcatgcta 
      gtccttagt gctttgatt ggtatttac tttggctgc 
      tttggaagt aaacaatca agtgccaac gagtatctc 
      atgggcgga aaaacaatg agggtcata ccaatcgct 
      atatctcta gtcgcaagt cacacttcc gggattact 
      ctccttgca cttccggcc gatatttac cggtatggg 
      gccgcctat tggctgggg ggcatctcc atggccatt 
      ctttccgta gtgacaatc tacgtctac ttaccggtg 
      ttctacaat ttacaaatt attagcacg tacgagtat 
      ttggaacga cgttttgat aagaagacg cgatcgttt 
      gcgtcgttt ctttacgca gtttcggtc tttttgtat 
      ttgccaatc gttgtctat attccagcc ttggcgttt 
      tcggccgct tcagggatt gacgtccac ttgatagct 
      cctgttgtt tgcgcagtt tgtattttt tacactacc 
      atagggggg cttaaagca gtcgtctgg actgacact 
      ctccagttt agtgtaaca gtgggggcc atcatcaca 
      gtctttatc ttgggcgtg aaatcagca ggtggtttc 
      ttgcaagtg tggaacaag gcagttgaa ggccaaaga 
      ttggatatt ttcgacttc gatttggac ccaacaaag 
      cgtgacagt ttctgggcc attgtgatc ggtttaacc 
      gtccactgg atggggcac accagtgtt aaccaaggc 
      tgtgtccag aaatttctc gcagtttcg actttgaaa 
      aaatccagt tttgttttg actaggttt atcatgttt 
      gccaaatac tctgactgt gatcctttc acaacgaaa 
      caagtgaca cgaaacgac cagttgctc ccttactac 
      gtgatggac gttgcccgg aacattccg gggctttcc 
      ggtcttttt attgccgga gttttcagt gcgggtttg 
      agcacactt tcagcaact ttgaactgt ttggctgga 
      actttatac gaggacttt atcagcaag tttatgcat 
      aaagggatt acggaaaaa tccgccagc aatattttg 
      aaaataatt gtgatagtc accggagtt gcgtgcacg 
      gttctggtg tttgtgatt gaacacatg ggagggtta 
      ttaaaattg tcgataagt ctaggaggg gttacaaac 
      ggccctctg ttgggtctg tttactttg ggaatgctt 
      tttccgaga gccaactcc aaaggggcg ttttatggc 
      acaatcggt ggtgtaatt ttcatttca acgttaatt 
      ttcccaact aaatatttt gagacgcaa ggattgatt 
      aaatatcct cctaaaccg ttgtcaacc gaacggtgc 
      gattttttt aacgagacg aatattttt gaaaatgtt 
      ttacacagg caagtttgc acgtttaac gttttgaaa 
      ataatttat tcaagttct tacaggaat agctcaact 
      tattttgaa cctcctgca attttccga atctcgttt 
      tattattac acgcttata ggggctctt tctactata 
      atttttgct ttaataatt agctacatg acaaataaa 
      aatgaccct ccggttgat aaatcgctg ataagtcca 
      gtgagccac tttttgctt cccaaagac actaaaacc 
      aatggtgtt gttgagtac aatactgtt gagagggcc 
      ctcgaagtt gttacaaca aactcagga aaagagaac 
      aaatgatta ttgtttgta gttgatttt tatttacct 
      attttaaat aaaatttct tttactaa