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Gene information
General information
TC002506 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26335026..26341455
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002506
    iBeetle screen iB_00392

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    egg chamber size irregular - Number of animals showing the phenotype: 3

    egg chamber shape irregular - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 2
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002506 RA
      MDENSENHT PDPKVPESP KPLAFTVDF GDGKAIDTQ 
      RHKSLVEKF QKKHRRGQS LSKLEEPQL GPARKHPNT 
      GNLPRKSSF QSEGYFSSD EKSDRVKSA KRGELTLPL 
      KSVVDKMSQ SFPNSGFFN IKSPEIELK DVSSPELDL 
      ISPFSPSQS KSNLAKQLP SPKKDAENL VEGTEFDFD 
      KSDTVSDAG TYTLDADNY SEEQKARMS IDAEFKIEQ 
      ISVLKKTED YIKSLNTYK QKNQPDITK TVQELNLND 
      TPNPPSPKS PKHSTPNVK NFTKTSAKM LSPILSPTQ 
      NLSITQDEE KNADLTNKT FTKIMFPSP KARNANGLE 
      QLVDQGSVI SVTSSGAFR SERPKPRKL SLTKSEVRV 
      EAYVGNKVE VSESTVRVG NPLKKLTAN LVNVESIST 
      NGVPVEENV VSGSLVLGK VTSPSKIPS PIHTLSRPR 
      SRNSANLDL SDSSLETES YLKPTQNCI NSLQQRLSL 
      DSDQESDYL NNSPHSLLK QRAIHIRHN SLDDKNMKI 
      SNKLEHFQS KNLQSIDQT FNVLNQYAK PIHKIQNSP 
      NNSPIRRSS SFTTKNPIP AKNTNVVLQ ETIRNSPNL 
      SRNTSIQRS ASTANIKPN FLQLRRSSI SNDHQKIDR 
      NQFGDTESS SEEDFEKNL SKKKDLSNL TNTRYNRAF 
      SLRRARLDE PVVSPKCPN TPEMRRKFQ PEHLRTERA 
      ISVDRKPVK TNEVQSRYL LNVTKKPPV KTEPVKTTV 
      PKSTTTKAQ VFSRTDNGR FSMRAAKPP TTGGQKPVK 
      KDANGKKSS GRSNSSLSS REKEFQNWK RRKSYDPMK 
      AAAEGKRKE MAKRQNSMT QSYTEANQN QDCDSSPSH 
      SSSVHRSQS FHGTAALEQ LISSEEEED LTLSADEGF 
      SPPTPSPCE LSPARASLR IAWKDHVLH
    CDS sequence
    • >TC002506 RA
      atggatgag aatagcgaa aatcacact cccgacccc 
      aaagtcccc gagtccccg aaaccgctc gctttcacc 
      gtcgatttc ggcgacggg aaggccata gacacgcaa 
      cgccacaaa agcctagtc gagaagttc caaaagaag 
      caccgcagg ggccagtcg ctgtccaag ctcgaggag 
      ccccagctg ggccccgcg cgcaagcac cccaacacc 
      gggaacctg ccccgcaag tcgagcttc caatccgag 
      ggctacttc tcctccgac gagaaaagt gaccgggtt 
      aaaagtgcg aaacggggc gagttgacg ttgcctttg 
      aaaagtgtc gtggacaaa atgagccag tcgtttcca 
      aacagtggg ttttttaac ataaaatcg ccagagatc 
      gaactgaag gatgtgtca agtccggaa ctggacttg 
      ataagtccg ttcagtccc tcgcagagt aagtctaac 
      ctcgctaaa cagttacca agtcctaaa aaggacgct 
      gagaattta gtcgaaggg actgagttt gattttgac 
      aagtctgac actgtcagt gacgctggc acctacaca 
      ctagatgct gacaattat tccgaggaa caaaaggca 
      cgaatgagc atcgatgcg gagttcaaa atcgaacaa 
      atctcagta ttgaaaaag accgaagat tacattaag 
      agcctcaac acgtacaag caaaaaaac cagcctgac 
      attactaaa accgtgcaa gaattaaac ttgaatgat 
      acccccaac cccccgtca ccaaaatcc ccaaaacat 
      agcacgcca aacgtcaaa aatttcaca aaaaccagc 
      gcaaaaatg ctatcaccg atcctgtct ccgacgcaa 
      aacctctca atcacgcaa gacgaggag aaaaacgcc 
      gatttgacc aacaagact ttcacgaaa attatgttc 
      ccaagccct aaggcacgc aacgccaat ggactcgaa 
      caacttgtt gatcaaggc agcgtcatt tcggtcacc 
      tcaagtggg gctttccgc agcgaaaga ccgaagccc 
      cgaaaattg agcttaaca aaatcggaa gtccgggtc 
      gaggcttac gttggcaac aaggtcgag gtcagcgaa 
      agtaccgtc agggttggc aacccccta aaaaagtta 
      acagcgaat ctggtcaat gttgagagt atttcgacg 
      aacggtgtc ccagttgag gaaaatgtg gttagtgga 
      agtctagtt ttgggaaaa gtcacttcg ccgtcgaaa 
      atcccgtcg ccgattcac acgttgtcg cggccgcga 
      agtcgcaat agtgccaac ttggacttg agcgatagc 
      agtttggag acggagtcg tacttgaaa ccgactcag 
      aattgcata aattcgctg cagcagaga ctgtcgctt 
      gatagcgac caggagagc gattatttg aacaattca 
      ccgcacagt ttattgaaa cagcgggcg attcacatt 
      cggcacaac tcacttgat gacaagaac atgaaaata 
      tcgaacaaa ctcgagcac ttccaaagc aagaacttg 
      caaagtatt gaccaaaca ttcaatgtg ttaaatcaa 
      tacgcaaag cccattcat aagatacag aattcgccc 
      aacaattcg ccgattcga cgctcgagt agttttacg 
      acgaagaac ccgattccg gcgaaaaac accaacgtg 
      gtgttacaa gaaacgata cgcaactcg cctaacctt 
      agtcggaac acttcgata caaagatcg gcctcaacg 
      gcaaacatc aaaccgaat ttcctccaa ctcagacgt 
      tcgagtatc tcgaacgac caccagaaa atcgaccgg 
      aatcaattc ggcgatacg gagtcgagt tccgaggag 
      gatttcgag aagaactta tcgaaaaag aaagatttg 
      tcgaatttg accaacacg aggtacaac cgggcgttt 
      agtctgcgg agggcgcgc ctcgatgag cctgtcgtt 
      tcaccaaag tgccccaac accccagaa atgcggcgc 
      aagttccag ccggagcat ctccggacg gagcgagcg 
      atttctgtg gataggaaa ccagtgaag acgaatgag 
      gttcaaagt aggtacctg ttgaatgtt accaagaaa 
      ccgccggtt aaaaccgaa ccggttaag actactgtg 
      ccaaaaagt acgacgacg aaggcgcaa gtgttttcg 
      aggacggac aatgggagg tttagtatg agggcggcg 
      aaaccgcca acgacgggg ggacaaaaa ccggtcaag 
      aaagatgcc aatggtaaa aaatctagc ggtcgtagc 
      aactcatcg ttatcaagt cgagagaaa gaatttcaa 
      aattggaaa cgacggaaa agctacgat ccgatgaaa 
      gcagccgcc gaaggcaag cgaaaagaa atggccaaa 
      agacaaaac agcatgaca cagtcatac acggaggcc 
      aatcagaat caagactgt gatagttcc ccgtcccat 
      tccagttcg gtgcatcgc tctcagagc tttcacggc 
      acagccgcc ctcgaacag ctcataagt tcagaggag 
      gaggaagac ttgaccctc tctgccgac gagggtttc 
      agtcctcca accccgagt ccttgcgaa ttgagtcca 
      gcaagggcg agtttgcga atcgcttgg aaggatcac 
      gtcctccat taa
    mRNA sequence
    • >TC002506 RA
      attaacaat aaataattt cccaacaaa aaagtgatg 
      tcactcccg ttggaagct agcatgtag ccgtggtgg 
      gacgaaacg tcaaacgtc agtcagttt gtccgcgcc 
      tacagctgt gaaaaatgc aacaaaaca tcaaataat 
      cagtttttc gtcgtaaaa aacttgcaa aaaagctca 
      agtgatgtg caattctcc gacctagct cgtcgtgcg 
      ccccaggtt gaaattggc gaaatagtt ccggcttga 
      atcatggat gagaatagc gaaaatcac actcccgac 
      cccaaagtc cccgagtcc ccgaaaccg ctcgctttc 
      accgtcgat ttcggcgac gggaaggcc atagacacg 
      caacgccac aaaagccta gtcgagaag ttccaaaag 
      aagcaccgc aggggccag tcgctgtcc aagctcgag 
      gagccccag ctgggcccc gcgcgcaag caccccaac 
      accgggaac ctgccccgc aagtcgagc ttccaatcc 
      gagggctac ttctcctcc gacgagaaa agtgaccgg 
      gttaaaagt gcgaaacgg ggcgagttg acgttgcct 
      ttgaaaagt gtcgtggac aaaatgagc cagtcgttt 
      ccaaacagt gggtttttt aacataaaa tcgccagag 
      atcgaactg aaggatgtg tcaagtccg gaactggac 
      ttgataagt ccgttcagt ccctcgcag agtaagtct 
      aacctcgct aaacagtta ccaagtcct aaaaaggac 
      gctgagaat ttagtcgaa gggactgag tttgatttt 
      gacaagtct gacactgtc agtgacgct ggcacctac 
      acactagat gctgacaat tattccgag gaacaaaag 
      gcacgaatg agcatcgat gcggagttc aaaatcgaa 
      caaatctca gtattgaaa aagaccgaa gattacatt 
      aagagcctc aacacgtac aagcaaaaa aaccagcct 
      gacattact aaaaccgtg caagaatta aacttgaat 
      gataccccc aaccccccg tcaccaaaa tccccaaaa 
      catagcacg ccaaacgtc aaaaatttc acaaaaacc 
      agcgcaaaa atgctatca ccgatcctg tctccgacg 
      caaaacctc tcaatcacg caagacgag gagaaaaac 
      gccgatttg accaacaag actttcacg aaaattatg 
      ttcccaagc cctaaggca cgcaacgcc aatggactc 
      gaacaactt gttgatcaa ggcagcgtc atttcggtc 
      acctcaagt ggggctttc cgcagcgaa agaccgaag 
      ccccgaaaa ttgagctta acaaaatcg gaagtccgg 
      gtcgaggct tacgttggc aacaaggtc gaggtcagc 
      gaaagtacc gtcagggtt ggcaacccc ctaaaaaag 
      ttaacagcg aatctggtc aatgttgag agtatttcg 
      acgaacggt gtcccagtt gaggaaaat gtggttagt 
      ggaagtcta gttttggga aaagtcact tcgccgtcg 
      aaaatcccg tcgccgatt cacacgttg tcgcggccg 
      cgaagtcgc aatagtgcc aacttggac ttgagcgat 
      agcagtttg gagacggag tcgtacttg aaaccgact 
      cagaattgc ataaattcg ctgcagcag agactgtcg 
      cttgatagc gaccaggag agcgattat ttgaacaat 
      tcaccgcac agtttattg aaacagcgg gcgattcac 
      attcggcac aactcactt gatgacaag aacatgaaa 
      atatcgaac aaactcgag cacttccaa agcaagaac 
      ttgcaaagt attgaccaa acattcaat gtgttaaat 
      caatacgca aagcccatt cataagata cagaattcg 
      cccaacaat tcgccgatt cgacgctcg agtagtttt 
      acgacgaag aacccgatt ccggcgaaa aacaccaac 
      gtggtgtta caagaaacg atacgcaac tcgcctaac 
      cttagtcgg aacacttcg atacaaaga tcggcctca 
      acggcaaac atcaaaccg aatttcctc caactcaga 
      cgttcgagt atctcgaac gaccaccag aaaatcgac 
      cggaatcaa ttcggcgat acggagtcg agttccgag 
      gaggatttc gagaagaac ttatcgaaa aagaaagat 
      ttgtcgaat ttgaccaac acgaggtac aaccgggcg 
      tttagtctg cggagggcg cgcctcgat gagcctgtc 
      gtttcacca aagtgcccc aacacccca gaaatgcgg 
      cgcaagttc cagccggag catctccgg acggagcga 
      gcgatttct gtggatagg aaaccagtg aagacgaat 
      gaggttcaa agtaggtac ctgttgaat gttaccaag 
      aaaccgccg gttaaaacc gaaccggtt aagactact 
      gtgccaaaa agtacgacg acgaaggcg caagtgttt 
      tcgaggacg gacaatggg aggtttagt atgagggcg 
      gcgaaaccg ccaacgacg gggggacaa aaaccggtc 
      aagaaagat gccaatggt aaaaaatct agcggtcgt 
      agcaactca tcgttatca agtcgagag aaagaattt 
      caaaattgg aaacgacgg aaaagctac gatccgatg 
      aaagcagcc gccgaaggc aagcgaaaa gaaatggcc 
      aaaagacaa aacagcatg acacagtca tacacggag 
      gccaatcag aatcaagac tgtgatagt tccccgtcc 
      cattccagt tcggtgcat cgctctcag agctttcac 
      ggcacagcc gccctcgaa cagctcata agttcagag 
      gaggaggaa gacttgacc ctctctgcc gacgagggt 
      ttcagtcct ccaaccccg agtccttgc gaattgagt 
      ccagcaagg gcgagtttg cgaatcgct tggaaggat 
      cacgtcctc cattaattt cgacgacat tcgaacgaa 
      tcattttgt tattattta ttacagtac ttaggtaat 
      gtttgcaca aaaacaacg gcagtcggt gcaatatac 
      atactaaag ctcaagatc caatcgcat gccaaattt 
      tttaaaaca actgccgaa attattttg ctgtgatat 
      cgtttgttt tattagttg taagcacaa ctgggggcg 
      gaaacagtg gcattacct acgtacgtg ttttgttta 
      gtgtgtaaa tatgaccat atcattgtt ttttctcgg 
      ttgtcgcac ttattattt ttgtgtttc attgcaaaa 
      aaagcaacg aagacgcga ttgcgggaa agacagttt 
      cgtacttca agaatctct tccaaagtt aagtaaaag 
      aaaagtaac caacatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY