| General information | |||
| TC002504 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 26359101..26368183 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002504 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00391 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
remarks - cuticles: eggs lost,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC002504 RA
MSEQSIASA RASVMIYDD VNKKWIPSG TSSGLSKVH IYQHTVQQT FRVVGRKLQ DHEVVINCA ILKGLKYNQ ATATFHQWR DNKQVYGLN FSSKEDADL FARAMFHSL EVLSNIVRQ PTPQYAPQM PPQIQQPAI PQQQYDEDM GYRTMTRED VAMIQERRI SNSLMSPQQ TSPMTPQNN VPVAPAMPM SVAAAAVPT PISPPSSGH QRTVSAPPA PPPPPGGPP MPPTAGAAP PPPPPPPMN MSRSQSSDG GEVNSLAAQ LQNARLKRN NKNTPPPTE NSGSSTSSG GSSNYGTLG RGGGGSMAS MMDEMAKTL ARRRAAVEK KIPDKDEEE KKAVWEKTN TLPSNTSKF SESPKSIRK RFGSASEET ILKVNGLSE VSGLSLGPT ELESLKNEI VKEVRKEIT KMKQDIIDA IKSELNRR
- >TC002504 RB
MDFDRDNLC KEIEEEFNV TLRKSLRES WQDYWNCEQ SIASARASV MIYDDVNKK WIPSGTSSG LSKVHIYQH TVQQTFRVV GRKLQDHEV VINCAILKG LKYNQATAT FHQWRDNKQ VYGLNFSSK EDADLFARA MFHSLEVLS NIVRQPTPQ YAPQMPPQI QQPAIPQQQ YDEDMGYRT MTREDVAMI QERRISNSL MSPQQTSPM TPQNNVPVA PAMPMSVAA AAVPTPISP PSSGHQRTV SAPPAPPPP PGGPPMPPT AGAAPPPPP PPPMNMSRS QSSDGGEVN SLAAQLQNA RLKRNNKNT PPPTENSGS STSSGGSSN YGTLGRGGG GSMASMMDE MAKTLARRR AAVEKKIPD KDEEEKKAV WEKTNTLPS NTSKFSESP KSIRKRFGS ASEETILKV NGLSEVSGL SLGPTELES LKNEIVKEV RKEITKMKQ DIIDAIKSE LNRR
- >TC002504 RA
atgagtgaa caatcgata gctagcgca agagctagt gttatgatc tacgatgac gtcaataag aaatggatt ccctcagga acatcgtcg ggattatca aaagttcac atataccaa catactgta caacagaca ttccgtgtc gtcggaaga aaactccag gatcatgaa gttgttata aattgtgcc attttaaaa ggactgaaa tacaatcaa gcaactgcc acatttcac cagtggcgt gacaataaa caggtttac gggttaaac ttcagctca aaagaagat gcggacctc ttcgccagg gcaatgttc cactcatta gaagtactt agtaatata gttcgacaa ccgaccccc caatacgcc ccccaaatg cccccacaa atccagcaa cctgcgatc ccccaacaa caatacgac gaagacatg gggtatcgc acgatgaca cgcgaagac gtcgccatg atccaggaa cggcggata tccaactcg ctgatgtcg ccccaacaa acatcgccg atgactccc cagaacaac gtgcctgtg gcaccggcg atgccaatg tcggtggcg gccgccgcc gtccccacg ccaatttca ccaccgtcg agcggccac caacggacg gtgagcgcg cccccggca ccgccacca cctccagga ggcccccca atgcccccg acagccgga gcagcaccg ccgccacca cccccaccc ccaatgaac atgtcgcgg tcgcagagc agcgacggc ggagaggtc aactccttg gcagcgcag ctccaaaac gcgaggttg aagaggaat aataagaat acgccgccc ccgactgag aacagcggc tcgtcgaca agcagcggt gggagtagt aattacggg acgctgggg cgaggaggt ggcggaagt atggcttcg atgatggac gaaatggct aaaactttg gctcgacga agagcggct gttgagaaa aaaatcccc gataaggat gaggaagag aaaaaggcc gtttgggag aaaacgaat acgttaccg agtaatacg tcaaagttt tccgaatca ccgaaaagt atccgaaaa cggttcggc tccgcttcc gaggagaca atcttaaaa gtgaacggc ctatcggaa gtttcaggg ctgtcgctt ggaccaacc gaattagaa agtctcaaa aacgaaata gtaaaagaa gtgaggaag gaaataacg aaaatgaaa caggatata atagacgca atcaaatca gaactgaac cgaaggtaa
- >TC002504 RB
atggatttc gatagggat aatttgtgt aaggagatt gaagaggaa tttaatgtt acgttgcgc aagagtctg cgagaaagc tggcaagat tactggaac tgtgaacaa tcgatagct agcgcaaga gctagtgtt atgatctac gatgacgtc aataagaaa tggattccc tcaggaaca tcgtcggga ttatcaaaa gttcacata taccaacat actgtacaa cagacattc cgtgtcgtc ggaagaaaa ctccaggat catgaagtt gttataaat tgtgccatt ttaaaagga ctgaaatac aatcaagca actgccaca tttcaccag tggcgtgac aataaacag gtttacggg ttaaacttc agctcaaaa gaagatgcg gacctcttc gccagggca atgttccac tcattagaa gtacttagt aatatagtt cgacaaccg accccccaa tacgccccc caaatgccc ccacaaatc cagcaacct gcgatcccc caacaacaa tacgacgaa gacatgggg tatcgcacg atgacacgc gaagacgtc gccatgatc caggaacgg cggatatcc aactcgctg atgtcgccc caacaaaca tcgccgatg actccccag aacaacgtg cctgtggca ccggcgatg ccaatgtcg gtggcggcc gccgccgtc cccacgcca atttcacca ccgtcgagc ggccaccaa cggacggtg agcgcgccc ccggcaccg ccaccacct ccaggaggc cccccaatg cccccgaca gccggagca gcaccgccg ccaccaccc ccaccccca atgaacatg tcgcggtcg cagagcagc gacggcgga gaggtcaac tccttggca gcgcagctc caaaacgcg aggttgaag aggaataat aagaatacg ccgcccccg actgagaac agcggctcg tcgacaagc agcggtggg agtagtaat tacgggacg ctggggcga ggaggtggc ggaagtatg gcttcgatg atggacgaa atggctaaa actttggct cgacgaaga gcggctgtt gagaaaaaa atccccgat aaggatgag gaagagaaa aaggccgtt tgggagaaa acgaatacg ttaccgagt aatacgtca aagttttcc gaatcaccg aaaagtatc cgaaaacgg ttcggctcc gcttccgag gagacaatc ttaaaagtg aacggccta tcggaagtt tcagggctg tcgcttgga ccaaccgaa ttagaaagt ctcaaaaac gaaatagta aaagaagtg aggaaggaa ataacgaaa atgaaacag gatataata gacgcaatc aaatcagaa ctgaaccga aggtaa
- >TC002504 RA
atggcggcg atggctgca taatgaaaa tttgtaaaa gtgactaag gtgagatta ttaaatgga gtttccaaa gataaaact caaacacat tccttgaag tgcgaagtg tcggtttag tgatgtgga cgacgtaat cgcaaattt attcgtcga ttttcatta aaaaaacat caaaatgag tgaacaatc gatagctag cgcaagagc tagtgttat gatctacga tgacgtcaa taagaaatg gattccctc aggaacatc gtcgggatt atcaaaagt tcacatata ccaacatac tgtacaaca gacattccg tgtcgtcgg aagaaaact ccaggatca tgaagttgt tataaattg tgccatttt aaaaggact gaaatacaa tcaagcaac tgccacatt tcaccagtg gcgtgacaa taaacaggt ttacgggtt aaacttcag ctcaaaaga agatgcgga cctcttcgc cagggcaat gttccactc attagaagt acttagtaa tatagttcg acaaccgac cccccaata cgcccccca aatgccccc acaaatcca gcaacctgc gatccccca acaacaata cgacgaaga catggggta tcgcacgat gacacgcga agacgtcgc catgatcca ggaacggcg gatatccaa ctcgctgat gtcgcccca acaaacatc gccgatgac tccccagaa caacgtgcc tgtggcacc ggcgatgcc aatgtcggt ggcggccgc cgccgtccc cacgccaat ttcaccacc gtcgagcgg ccaccaacg gacggtgag cgcgccccc ggcaccgcc accacctcc aggaggccc cccaatgcc cccgacagc cggagcagc accgccgcc accaccccc acccccaat gaacatgtc gcggtcgca gagcagcga cggcggaga ggtcaactc cttggcagc gcagctcca aaacgcgag gttgaagag gaataataa gaatacgcc gcccccgac tgagaacag cggctcgtc gacaagcag cggtgggag tagtaatta cgggacgct ggggcgagg aggtggcgg aagtatggc ttcgatgat ggacgaaat ggctaaaac tttggctcg acgaagagc ggctgttga gaaaaaaat ccccgataa ggatgagga agagaaaaa ggccgtttg ggagaaaac gaatacgtt accgagtaa tacgtcaaa gttttccga atcaccgaa aagtatccg aaaacggtt cggctccgc ttccgagga gacaatctt aaaagtgaa cggcctatc ggaagtttc agggctgtc gcttggacc aaccgaatt agaaagtct caaaaacga aatagtaaa agaagtgag gaaggaaat aacgaaaat gaaacagga tataataga cgcaatcaa atcagaact gaaccgaag gtaatttct tattgcctt attttgtgc gtgaaattt ttttaaagt tttacactt ggaacaaat catgcgaca gtttctaac attaaacag tagtaatcg gtattaacg taaacaatg tacgtttat ggaacgagt atgttatta atgtttcct atttgctgt tctgtacgt ttttcgtaa ttattagtc aacggcgtt gatcgacgg gtctagaca catgaggtt gaatctttt taattatta caataatta tttaatagc tttatttgt gatggacga tgcatgtgt caagtacag aatagcgga attttaaag taatgctaa tagatgtcg tggatatag agcactttt attataagt tctaacata gtatttatt ttattataa gttaattag gcatttaaa gattttcag tgattccta tgtaacacc taatcaaac agtgctaca acttttgta aatatttat ataggactt ccaatgacc tacatatgt atttattaa cagctgatt cgttgccct agaaaacgc caaaaaatt gtcttaccc cgagtggtt gtacgcatt taatgcttc tttggtgcc aacaatgta agacagttt tcgggccac ttttttcta ttttgttca aaatgaagg gaaacaatg atcgtgtgt atattatag aatcataag ttaatagat gcttattag attagtgtc aaatcatat atacatcga acgtttcca tttatcatt cttcacatt ttatatgaa aacaaacca ttggtaatc ctgcatcag acggcagtt ttgtacact ctcgctgtc gtgtttgtg aatagtgga ttgttatcg aaagctcac ccaaaaaac gacgcattg agcgaaaaa tattgtatt acatacaga cataatcag tgattgcaa ttttatata taatttctt aagtacata tgttataac tttgtgata tataactgc atttgagtt tattgtata ttgtctagc acttttttg ttttgtctt cttagattt attttgtat gtgatctca agtcaaaat tagcacaat ttttctaat tttttatat ttaaaaaaa tcgatttta attcgtaac tacgattac gtttcaaat tttacaatg tatttgaaa taaacattt gaagtacgt
- >TC002504 RB
atggcggcg atggctgca taatgaaaa tttgtaaaa gtgactaag gtgagatta ttaaatgga gtttccaaa gataaaact caaacacat tccttgaag tgcgaagtg tcggtttag tgatgtgga cgacgtaat cgcaaattt attcgtcga ttttcatta aaaaaacat caaaatgag ggccagttg gagtcggtg tttgtgtat gggtcgtgt cagtcgcac ataaacaaa gcgtacgac cctcctgct tcatattaa tattgaact ttgctacca aacttcctg attaatact acaactatc tgtgtcagc ataatttcc agtcggttg tggcttcgc ttttggttc acgtgtggt tttttattt ttcttcgtg taataaaaa ttgtttgtg tgattggtc gtcgaaatg gatttcgat agggataat ttgtgtaag gagattgaa gaggaattt aatgttacg ttgcgcaag agtctgcga gaaagctgg caagattac tggaactgt gaacaatcg atagctagc gcaagagct agtgttatg atctacgat gacgtcaat aagaaatgg attccctca ggaacatcg tcgggatta tcaaaagtt cacatatac caacatact gtacaacag acattccgt gtcgtcgga agaaaactc caggatcat gaagttgtt ataaattgt gccatttta aaaggactg aaatacaat caagcaact gccacattt caccagtgg cgtgacaat aaacaggtt tacgggtta aacttcagc tcaaaagaa gatgcggac ctcttcgcc agggcaatg ttccactca ttagaagta cttagtaat atagttcga caaccgacc ccccaatac gccccccaa atgccccca caaatccag caacctgcg atcccccaa caacaatac gacgaagac atggggtat cgcacgatg acacgcgaa gacgtcgcc atgatccag gaacggcgg atatccaac tcgctgatg tcgccccaa caaacatcg ccgatgact ccccagaac aacgtgcct gtggcaccg gcgatgcca atgtcggtg gcggccgcc gccgtcccc acgccaatt tcaccaccg tcgagcggc caccaacgg acggtgagc gcgcccccg gcaccgcca ccacctcca ggaggcccc ccaatgccc ccgacagcc ggagcagca ccgccgcca ccaccccca cccccaatg aacatgtcg cggtcgcag agcagcgac ggcggagag gtcaactcc ttggcagcg cagctccaa aacgcgagg ttgaagagg aataataag aatacgccg cccccgact gagaacagc ggctcgtcg acaagcagc ggtgggagt agtaattac gggacgctg gggcgagga ggtggcgga agtatggct tcgatgatg gacgaaatg gctaaaact ttggctcga cgaagagcg gctgttgag aaaaaaatc cccgataag gatgaggaa gagaaaaag gccgtttgg gagaaaacg aatacgtta ccgagtaat acgtcaaag ttttccgaa tcaccgaaa agtatccga aaacggttc ggctccgct tccgaggag acaatctta aaagtgaac ggcctatcg gaagtttca gggctgtcg cttggacca accgaatta gaaagtctc aaaaacgaa atagtaaaa gaagtgagg aaggaaata acgaaaatg aaacaggat ataatagac gcaatcaaa tcagaactg aaccgaagg taatttctt attgcctta ttttgtgcg tgaaatttt tttaaagtt ttacacttg gaacaaatc atgcgacag tttctaaca ttaaacagt agtaatcgg tattaacgt aaacaatgt acgtttatg gaacgagta tgttattaa tgtttccta tttgctgtt ctgtacgtt tttcgtaat tattagtca acggcgttg atcgacggg tctagacac atgaggttg aatcttttt aattattac aataattat ttaatagct ttatttgtg atggacgat gcatgtgtc aagtacaga atagcggaa ttttaaagt aatgctaat agatgtcgt ggatataga gcactttta ttataagtt ctaacatag tatttattt tattataag ttaattagg catttaaag attttcagt gattcctat gtaacacct aatcaaaca gtgctacaa cttttgtaa atatttata taggacttc caatgacct acatatgta tttattaac agctgattc gttgcccta gaaaacgcc aaaaaattg tcttacccc gagtggttg tacgcattt aatgcttct ttggtgcca acaatgtaa gacagtttt cgggccact tttttctat tttgttcaa aatgaaggg aaacaatga tcgtgtgta tattataga atcataagt taatagatg cttattaga ttagtgtca aatcatata tacatcgaa cgtttccat ttatcattc ttcacattt tatatgaaa acaaaccat tggtaatcc tgcatcaga cggcagttt tgtacactc tcgctgtcg tgtttgtga atagtggat tgttatcga aagctcacc caaaaaacg acgcattga gcgaaaaat attgtatta catacagac ataatcagt gattgcaat tttatatat aatttctta agtacatat gttataact ttgtgatat ataactgca tttgagttt attgtatat tgtctagca cttttttgt tttgtcttc ttagattta ttttgtatg tgatctcaa gtcaaaatt agcacaatt tttctaatt ttttatatt taaaaaaat cgattttaa ttcgtaact acgattacg tttcaaatt ttacaatgt atttgaaat aaacatttg aagtacgt
