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Gene information
General information
TC002504 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 995185..1004267
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002504
    iBeetle screen iB_00391
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002504 RA
      MSEQSIASA RASVMIYDD VNKKWIPSG TSSGLSKVH 
      IYQHTVQQT FRVVGRKLQ DHEVVINCA ILKGLKYNQ 
      ATATFHQWR DNKQVYGLN FSSKEDADL FARAMFHSL 
      EVLSNIVRQ PTPQYAPQM PPQIQQPAI PQQQYDEDM 
      GYRTMTRED VAMIQERRI SNSLMSPQQ TSPMTPQNN 
      VPVAPAMPM SVAAAAVPT PISPPSSGH QRTVSAPPA 
      PPPPPGGPP MPPTAGAAP PPPPPPPMN MSRSQSSDG 
      GEVNSLAAQ LQNARLKRN NKNTPPPTE NSGSSTSSG 
      GSSNYGTLG RGGGGSMAS MMDEMAKTL ARRRAAVEK 
      KIPDKDEEE KKAVWEKTN TLPSNTSKF SESPKSIRK 
      RFGSASEET ILKVNGLSE VSGLSLGPT ELESLKNEI 
      VKEVRKEIT KMKQDIIDA IKSELNRR
    • >TC002504 RB
      MDFDRDNLC KEIEEEFNV TLRKSLRES WQDYWNCEQ 
      SIASARASV MIYDDVNKK WIPSGTSSG LSKVHIYQH 
      TVQQTFRVV GRKLQDHEV VINCAILKG LKYNQATAT 
      FHQWRDNKQ VYGLNFSSK EDADLFARA MFHSLEVLS 
      NIVRQPTPQ YAPQMPPQI QQPAIPQQQ YDEDMGYRT 
      MTREDVAMI QERRISNSL MSPQQTSPM TPQNNVPVA 
      PAMPMSVAA AAVPTPISP PSSGHQRTV SAPPAPPPP 
      PGGPPMPPT AGAAPPPPP PPPMNMSRS QSSDGGEVN 
      SLAAQLQNA RLKRNNKNT PPPTENSGS STSSGGSSN 
      YGTLGRGGG GSMASMMDE MAKTLARRR AAVEKKIPD 
      KDEEEKKAV WEKTNTLPS NTSKFSESP KSIRKRFGS 
      ASEETILKV NGLSEVSGL SLGPTELES LKNEIVKEV 
      RKEITKMKQ DIIDAIKSE LNRR
    CDS sequence
    • >TC002504 RA
      atgagtgaa caatcgata gctagcgca agagctagt 
      gttatgatc tacgatgac gtcaataag aaatggatt 
      ccctcagga acatcgtcg ggattatca aaagttcac 
      atataccaa catactgta caacagaca ttccgtgtc 
      gtcggaaga aaactccag gatcatgaa gttgttata 
      aattgtgcc attttaaaa ggactgaaa tacaatcaa 
      gcaactgcc acatttcac cagtggcgt gacaataaa 
      caggtttac gggttaaac ttcagctca aaagaagat 
      gcggacctc ttcgccagg gcaatgttc cactcatta 
      gaagtactt agtaatata gttcgacaa ccgaccccc 
      caatacgcc ccccaaatg cccccacaa atccagcaa 
      cctgcgatc ccccaacaa caatacgac gaagacatg 
      gggtatcgc acgatgaca cgcgaagac gtcgccatg 
      atccaggaa cggcggata tccaactcg ctgatgtcg 
      ccccaacaa acatcgccg atgactccc cagaacaac 
      gtgcctgtg gcaccggcg atgccaatg tcggtggcg 
      gccgccgcc gtccccacg ccaatttca ccaccgtcg 
      agcggccac caacggacg gtgagcgcg cccccggca 
      ccgccacca cctccagga ggcccccca atgcccccg 
      acagccgga gcagcaccg ccgccacca cccccaccc 
      ccaatgaac atgtcgcgg tcgcagagc agcgacggc 
      ggagaggtc aactccttg gcagcgcag ctccaaaac 
      gcgaggttg aagaggaat aataagaat acgccgccc 
      ccgactgag aacagcggc tcgtcgaca agcagcggt 
      gggagtagt aattacggg acgctgggg cgaggaggt 
      ggcggaagt atggcttcg atgatggac gaaatggct 
      aaaactttg gctcgacga agagcggct gttgagaaa 
      aaaatcccc gataaggat gaggaagag aaaaaggcc 
      gtttgggag aaaacgaat acgttaccg agtaatacg 
      tcaaagttt tccgaatca ccgaaaagt atccgaaaa 
      cggttcggc tccgcttcc gaggagaca atcttaaaa 
      gtgaacggc ctatcggaa gtttcaggg ctgtcgctt 
      ggaccaacc gaattagaa agtctcaaa aacgaaata 
      gtaaaagaa gtgaggaag gaaataacg aaaatgaaa 
      caggatata atagacgca atcaaatca gaactgaac 
      cgaaggtaa
    • >TC002504 RB
      atggatttc gatagggat aatttgtgt aaggagatt 
      gaagaggaa tttaatgtt acgttgcgc aagagtctg 
      cgagaaagc tggcaagat tactggaac tgtgaacaa 
      tcgatagct agcgcaaga gctagtgtt atgatctac 
      gatgacgtc aataagaaa tggattccc tcaggaaca 
      tcgtcggga ttatcaaaa gttcacata taccaacat 
      actgtacaa cagacattc cgtgtcgtc ggaagaaaa 
      ctccaggat catgaagtt gttataaat tgtgccatt 
      ttaaaagga ctgaaatac aatcaagca actgccaca 
      tttcaccag tggcgtgac aataaacag gtttacggg 
      ttaaacttc agctcaaaa gaagatgcg gacctcttc 
      gccagggca atgttccac tcattagaa gtacttagt 
      aatatagtt cgacaaccg accccccaa tacgccccc 
      caaatgccc ccacaaatc cagcaacct gcgatcccc 
      caacaacaa tacgacgaa gacatgggg tatcgcacg 
      atgacacgc gaagacgtc gccatgatc caggaacgg 
      cggatatcc aactcgctg atgtcgccc caacaaaca 
      tcgccgatg actccccag aacaacgtg cctgtggca 
      ccggcgatg ccaatgtcg gtggcggcc gccgccgtc 
      cccacgcca atttcacca ccgtcgagc ggccaccaa 
      cggacggtg agcgcgccc ccggcaccg ccaccacct 
      ccaggaggc cccccaatg cccccgaca gccggagca 
      gcaccgccg ccaccaccc ccaccccca atgaacatg 
      tcgcggtcg cagagcagc gacggcgga gaggtcaac 
      tccttggca gcgcagctc caaaacgcg aggttgaag 
      aggaataat aagaatacg ccgcccccg actgagaac 
      agcggctcg tcgacaagc agcggtggg agtagtaat 
      tacgggacg ctggggcga ggaggtggc ggaagtatg 
      gcttcgatg atggacgaa atggctaaa actttggct 
      cgacgaaga gcggctgtt gagaaaaaa atccccgat 
      aaggatgag gaagagaaa aaggccgtt tgggagaaa 
      acgaatacg ttaccgagt aatacgtca aagttttcc 
      gaatcaccg aaaagtatc cgaaaacgg ttcggctcc 
      gcttccgag gagacaatc ttaaaagtg aacggccta 
      tcggaagtt tcagggctg tcgcttgga ccaaccgaa 
      ttagaaagt ctcaaaaac gaaatagta aaagaagtg 
      aggaaggaa ataacgaaa atgaaacag gatataata 
      gacgcaatc aaatcagaa ctgaaccga aggtaa
    mRNA sequence
    • >TC002504 RA
      atggcggcg atggctgca taatgaaaa tttgtaaaa 
      gtgactaag gtgagatta ttaaatgga gtttccaaa 
      gataaaact caaacacat tccttgaag tgcgaagtg 
      tcggtttag tgatgtgga cgacgtaat cgcaaattt 
      attcgtcga ttttcatta aaaaaacat caaaatgag 
      tgaacaatc gatagctag cgcaagagc tagtgttat 
      gatctacga tgacgtcaa taagaaatg gattccctc 
      aggaacatc gtcgggatt atcaaaagt tcacatata 
      ccaacatac tgtacaaca gacattccg tgtcgtcgg 
      aagaaaact ccaggatca tgaagttgt tataaattg 
      tgccatttt aaaaggact gaaatacaa tcaagcaac 
      tgccacatt tcaccagtg gcgtgacaa taaacaggt 
      ttacgggtt aaacttcag ctcaaaaga agatgcgga 
      cctcttcgc cagggcaat gttccactc attagaagt 
      acttagtaa tatagttcg acaaccgac cccccaata 
      cgcccccca aatgccccc acaaatcca gcaacctgc 
      gatccccca acaacaata cgacgaaga catggggta 
      tcgcacgat gacacgcga agacgtcgc catgatcca 
      ggaacggcg gatatccaa ctcgctgat gtcgcccca 
      acaaacatc gccgatgac tccccagaa caacgtgcc 
      tgtggcacc ggcgatgcc aatgtcggt ggcggccgc 
      cgccgtccc cacgccaat ttcaccacc gtcgagcgg 
      ccaccaacg gacggtgag cgcgccccc ggcaccgcc 
      accacctcc aggaggccc cccaatgcc cccgacagc 
      cggagcagc accgccgcc accaccccc acccccaat 
      gaacatgtc gcggtcgca gagcagcga cggcggaga 
      ggtcaactc cttggcagc gcagctcca aaacgcgag 
      gttgaagag gaataataa gaatacgcc gcccccgac 
      tgagaacag cggctcgtc gacaagcag cggtgggag 
      tagtaatta cgggacgct ggggcgagg aggtggcgg 
      aagtatggc ttcgatgat ggacgaaat ggctaaaac 
      tttggctcg acgaagagc ggctgttga gaaaaaaat 
      ccccgataa ggatgagga agagaaaaa ggccgtttg 
      ggagaaaac gaatacgtt accgagtaa tacgtcaaa 
      gttttccga atcaccgaa aagtatccg aaaacggtt 
      cggctccgc ttccgagga gacaatctt aaaagtgaa 
      cggcctatc ggaagtttc agggctgtc gcttggacc 
      aaccgaatt agaaagtct caaaaacga aatagtaaa 
      agaagtgag gaaggaaat aacgaaaat gaaacagga 
      tataataga cgcaatcaa atcagaact gaaccgaag 
      gtaatttct tattgcctt attttgtgc gtgaaattt 
      ttttaaagt tttacactt ggaacaaat catgcgaca 
      gtttctaac attaaacag tagtaatcg gtattaacg 
      taaacaatg tacgtttat ggaacgagt atgttatta 
      atgtttcct atttgctgt tctgtacgt ttttcgtaa 
      ttattagtc aacggcgtt gatcgacgg gtctagaca 
      catgaggtt gaatctttt taattatta caataatta 
      tttaatagc tttatttgt gatggacga tgcatgtgt 
      caagtacag aatagcgga attttaaag taatgctaa 
      tagatgtcg tggatatag agcactttt attataagt 
      tctaacata gtatttatt ttattataa gttaattag 
      gcatttaaa gattttcag tgattccta tgtaacacc 
      taatcaaac agtgctaca acttttgta aatatttat 
      ataggactt ccaatgacc tacatatgt atttattaa 
      cagctgatt cgttgccct agaaaacgc caaaaaatt 
      gtcttaccc cgagtggtt gtacgcatt taatgcttc 
      tttggtgcc aacaatgta agacagttt tcgggccac 
      ttttttcta ttttgttca aaatgaagg gaaacaatg 
      atcgtgtgt atattatag aatcataag ttaatagat 
      gcttattag attagtgtc aaatcatat atacatcga 
      acgtttcca tttatcatt cttcacatt ttatatgaa 
      aacaaacca ttggtaatc ctgcatcag acggcagtt 
      ttgtacact ctcgctgtc gtgtttgtg aatagtgga 
      ttgttatcg aaagctcac ccaaaaaac gacgcattg 
      agcgaaaaa tattgtatt acatacaga cataatcag 
      tgattgcaa ttttatata taatttctt aagtacata 
      tgttataac tttgtgata tataactgc atttgagtt 
      tattgtata ttgtctagc acttttttg ttttgtctt 
      cttagattt attttgtat gtgatctca agtcaaaat 
      tagcacaat ttttctaat tttttatat ttaaaaaaa 
      tcgatttta attcgtaac tacgattac gtttcaaat 
      tttacaatg tatttgaaa taaacattt gaagtacgt
    • >TC002504 RB
      atggcggcg atggctgca taatgaaaa tttgtaaaa 
      gtgactaag gtgagatta ttaaatgga gtttccaaa 
      gataaaact caaacacat tccttgaag tgcgaagtg 
      tcggtttag tgatgtgga cgacgtaat cgcaaattt 
      attcgtcga ttttcatta aaaaaacat caaaatgag 
      ggccagttg gagtcggtg tttgtgtat gggtcgtgt 
      cagtcgcac ataaacaaa gcgtacgac cctcctgct 
      tcatattaa tattgaact ttgctacca aacttcctg 
      attaatact acaactatc tgtgtcagc ataatttcc 
      agtcggttg tggcttcgc ttttggttc acgtgtggt 
      tttttattt ttcttcgtg taataaaaa ttgtttgtg 
      tgattggtc gtcgaaatg gatttcgat agggataat 
      ttgtgtaag gagattgaa gaggaattt aatgttacg 
      ttgcgcaag agtctgcga gaaagctgg caagattac 
      tggaactgt gaacaatcg atagctagc gcaagagct 
      agtgttatg atctacgat gacgtcaat aagaaatgg 
      attccctca ggaacatcg tcgggatta tcaaaagtt 
      cacatatac caacatact gtacaacag acattccgt 
      gtcgtcgga agaaaactc caggatcat gaagttgtt 
      ataaattgt gccatttta aaaggactg aaatacaat 
      caagcaact gccacattt caccagtgg cgtgacaat 
      aaacaggtt tacgggtta aacttcagc tcaaaagaa 
      gatgcggac ctcttcgcc agggcaatg ttccactca 
      ttagaagta cttagtaat atagttcga caaccgacc 
      ccccaatac gccccccaa atgccccca caaatccag 
      caacctgcg atcccccaa caacaatac gacgaagac 
      atggggtat cgcacgatg acacgcgaa gacgtcgcc 
      atgatccag gaacggcgg atatccaac tcgctgatg 
      tcgccccaa caaacatcg ccgatgact ccccagaac 
      aacgtgcct gtggcaccg gcgatgcca atgtcggtg 
      gcggccgcc gccgtcccc acgccaatt tcaccaccg 
      tcgagcggc caccaacgg acggtgagc gcgcccccg 
      gcaccgcca ccacctcca ggaggcccc ccaatgccc 
      ccgacagcc ggagcagca ccgccgcca ccaccccca 
      cccccaatg aacatgtcg cggtcgcag agcagcgac 
      ggcggagag gtcaactcc ttggcagcg cagctccaa 
      aacgcgagg ttgaagagg aataataag aatacgccg 
      cccccgact gagaacagc ggctcgtcg acaagcagc 
      ggtgggagt agtaattac gggacgctg gggcgagga 
      ggtggcgga agtatggct tcgatgatg gacgaaatg 
      gctaaaact ttggctcga cgaagagcg gctgttgag 
      aaaaaaatc cccgataag gatgaggaa gagaaaaag 
      gccgtttgg gagaaaacg aatacgtta ccgagtaat 
      acgtcaaag ttttccgaa tcaccgaaa agtatccga 
      aaacggttc ggctccgct tccgaggag acaatctta 
      aaagtgaac ggcctatcg gaagtttca gggctgtcg 
      cttggacca accgaatta gaaagtctc aaaaacgaa 
      atagtaaaa gaagtgagg aaggaaata acgaaaatg 
      aaacaggat ataatagac gcaatcaaa tcagaactg 
      aaccgaagg taatttctt attgcctta ttttgtgcg 
      tgaaatttt tttaaagtt ttacacttg gaacaaatc 
      atgcgacag tttctaaca ttaaacagt agtaatcgg 
      tattaacgt aaacaatgt acgtttatg gaacgagta 
      tgttattaa tgtttccta tttgctgtt ctgtacgtt 
      tttcgtaat tattagtca acggcgttg atcgacggg 
      tctagacac atgaggttg aatcttttt aattattac 
      aataattat ttaatagct ttatttgtg atggacgat 
      gcatgtgtc aagtacaga atagcggaa ttttaaagt 
      aatgctaat agatgtcgt ggatataga gcactttta 
      ttataagtt ctaacatag tatttattt tattataag 
      ttaattagg catttaaag attttcagt gattcctat 
      gtaacacct aatcaaaca gtgctacaa cttttgtaa 
      atatttata taggacttc caatgacct acatatgta 
      tttattaac agctgattc gttgcccta gaaaacgcc 
      aaaaaattg tcttacccc gagtggttg tacgcattt 
      aatgcttct ttggtgcca acaatgtaa gacagtttt 
      cgggccact tttttctat tttgttcaa aatgaaggg 
      aaacaatga tcgtgtgta tattataga atcataagt 
      taatagatg cttattaga ttagtgtca aatcatata 
      tacatcgaa cgtttccat ttatcattc ttcacattt 
      tatatgaaa acaaaccat tggtaatcc tgcatcaga 
      cggcagttt tgtacactc tcgctgtcg tgtttgtga 
      atagtggat tgttatcga aagctcacc caaaaaacg 
      acgcattga gcgaaaaat attgtatta catacagac 
      ataatcagt gattgcaat tttatatat aatttctta 
      agtacatat gttataact ttgtgatat ataactgca 
      tttgagttt attgtatat tgtctagca cttttttgt 
      tttgtcttc ttagattta ttttgtatg tgatctcaa 
      gtcaaaatt agcacaatt tttctaatt ttttatatt 
      taaaaaaat cgattttaa ttcgtaact acgattacg 
      tttcaaatt ttacaatgt atttgaaat aaacatttg 
      aagtacgt