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Gene information
General information
TC002501 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 1023235..1033257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002501
    iBeetle screen iB_00390
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary size decreased - Number of animals showing the phenotype: 4

    ovariole mostly not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 25.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002501 RA
      MAATRKLQG EIDRCLKKV TEGVETFED IWQKVHNAT 
      NSNQKEKYE ADLKKEIKK LQRLRDQIK SWIASAEIK 
      DKSVLMENR KLIETQMER FKVVERETK TKAYSKEGL 
      GAAQKLDPA QKEREEIQH WLVSSIDLL NIQTDTFEC 
      EIESLLANK KKKLDKDKQ ERMDELKSR LERHRFHIR 
      KLETLLRML DNMSVEVSQ IRRIKDNVE YYIECSQEP 
      DFEDNEFIY DDIIGLDEV ELSGVGLPS SATTDSNET 
      GGTPTSIIS GSSPVTSPA LGGQPYTHS SDSSNEADK 
      KTIFKDALK PVKPTAVRA TSAMNSSTN SILSYSCNS 
      TTSTPSSKP VLVSSTPSK QSAREASPV AQLNSTGNF 
      ATVAATTTA SKVTATNGE PVMSSVITN NNQVQTQNN 
      TAVVTSSHT QNSTNAHST QNSNTVSQK FNEQHVQNS 
      SISPELNNS SRSSPMTNT EVQHIQMMN GPTTESNKI 
      ADGMSSLKT MAQQVIDRA GLESNQQQV ILEANKIHN 
      QEKSDAHIP PLLGVAPLG TIPLQKEHQ AQFQLMEGA 
      YYHMPHPSD SEKVRTYLP RNSYPTPPY YIQTPLPHS 
      DTVEFYQRL ANETLFFVF YYMEGTKAQ YLAAKALKK 
      QSWRFHTKY MMWFQRHEE PKIINEEYE QGTYIYFDY 
      EKWGQRKKE GFTFEYKYL EDRDLN
    CDS sequence
    • >TC002501 RA
      atggctgct accagaaaa ttacaagga gagatcgat 
      cgatgtctc aagaaagtg actgaaggt gtggaaacg 
      ttcgaggat atctggcag aaggtccac aatgccact 
      aacagcaac cagaaagag aaatacgaa gccgatctc 
      aagaaggag attaagaag ctgcaaagg cttcgagac 
      cagattaaa agttggata gcttcggcc gaaatcaag 
      gataaaagt gttcttatg gaaaaccga aagttaata 
      gagactcaa atggagcga ttcaaagtg gttgagagg 
      gaaaccaag acaaaggcg tactcaaaa gagggcttg 
      ggggcggcg caaaagctc gatccagcg caaaaggaa 
      cgtgaagag atccagcat tggttagtt tcgtccatc 
      gacttgtta aacatccaa acggacact ttcgaatgc 
      gagatcgag tcgttgtta gcgaataag aaaaagaaa 
      ctcgataaa gacaaacag gagcgaatg gatgagctg 
      aagtcgcgc ttagaaagg cacaggttt cacatacga 
      aagttagaa actttgtta cgtatgctc gataatatg 
      tcggtggaa gtttcgcaa atacgaagg ataaaagac 
      aacgtcgaa tattacata gagtgttcg caagagccc 
      gatttcgag gataacgaa ttcatttac gatgacatc 
      attggcttg gatgaggtc gagttgtcg ggcgtgggg 
      ctgccgtcg tctgcgacg acggatagc aacgaaacg 
      ggcggaaca ccaacttcg attatttcg ggatcgtca 
      cctgtgacc tcgccggcg ttaggggga caaccctac 
      acccattcc agtgatagt tcaaatgaa gctgataag 
      aaaaccatt ttcaaagac gcgcttaaa cctgttaaa 
      ccaactgct gtccgagcg acgtccgcc atgaacagt 
      agtaccaac tctatcctg agctatagt tgcaatagt 
      acgacctca acacctagc agcaaacca gttctagta 
      agtagtact cccagcaag caatcggct cgagaagca 
      tcaccggtg gcgcaatta aattcgacc ggaaacttt 
      gccacagtt gctgcaaca acaacagcg tcgaaagtg 
      accgcaact aatggggaa cccgtcatg tcttctgtg 
      ataactaac aataatcaa gttcaaacg caaaacaat 
      acggccgtc gtaacgtcg tcgcatact caaaattcg 
      acaaatgcg cactcgacg caaaactca aacaccgta 
      tctcagaaa ttcaacgaa caacatgtg caaaatagt 
      agtattagt cccgaactt aataacagc agcaggtcc 
      tcgccaatg actaatacg gaagtacaa cacatacag 
      atgatgaac ggaccaacg accgaaagt aataagatt 
      gcggacggc atgtcgtct ctgaagacg atggcgcag 
      caggtgatc gaccgcgcg ggcttggag agcaaccag 
      cagcaggtg attttagaa gcgaacaaa atccataat 
      caggagaaa tcggacgcg cacatcccc ccgttattg 
      ggggtggcg ccgctcgga acgataccg ctccaaaaa 
      gagcaccag gcgcagttc cagctcatg gaaggtgcc 
      tattatcac atgccacat ccatccgat tccgaaaaa 
      gtaagaact tacttgcca aggaactca tatccaaca 
      ccaccttat tatatacaa acaccatta ccacattcc 
      gataccgtg gaattttat cagcgtttg gcgaacgaa 
      acattattt tttgttttt tattacatg gagggcaca 
      aaagctcaa tatttggcc gcgaaagcg cttaaaaag 
      caaagttgg aggtttcat acgaagtat atgatgtgg 
      ttccagagg cacgaagag cccaaaatt attaacgaa 
      gagtacgaa cagggaact tacatttac ttcgattat 
      gaaaagtgg ggccaacga aaaaaagaa ggatttacc 
      tttgaatat aagtactta gaggataga gacctgaac 
      tga
    mRNA sequence
    • >TC002501 RA
      agtgagaga catttgtgt atggcagag aattttcta 
      gttggttcg gaaaaagtg ggaaatatt ttctgaatt 
      taagccaat ttatgtaat aagtgatcc actattcga 
      agctctatt gcatggcga agtacttct aagtgtttg 
      gaattacca gtttcgtgg cgtttttaa ttatgaact 
      gattatatc cgacatggc tgctaccag aaaattaca 
      aggagagat cgatcgatg tctcaagaa agtgactga 
      aggtgtgga aacgttcga ggatatctg gcagaaggt 
      ccacaatgc cactaacag caaccagaa agagaaata 
      cgaagccga tctcaagaa ggagattaa gaagctgca 
      aaggcttcg agaccagat taaaagttg gatagcttc 
      ggccgaaat caaggataa aagtgttct tatggaaaa 
      ccgaaagtt aatagagac tcaaatgga gcgattcaa 
      agtggttga gagggaaac caagacaaa ggcgtactc 
      aaaagaggg cttgggggc ggcgcaaaa gctcgatcc 
      agcgcaaaa ggaacgtga agagatcca gcattggtt 
      agtttcgtc catcgactt gttaaacat ccaaacgga 
      cactttcga atgcgagat cgagtcgtt gttagcgaa 
      taagaaaaa gaaactcga taaagacaa acaggagcg 
      aatggatga gctgaagtc gcgcttaga aaggcacag 
      gtttcacat acgaaagtt agaaacttt gttacgtat 
      gctcgataa tatgtcggt ggaagtttc gcaaatacg 
      aaggataaa agacaacgt cgaatatta catagagtg 
      ttcgcaaga gcccgattt cgaggataa cgaattcat 
      ttacgatga catcattgg cttggatga ggtcgagtt 
      gtcgggcgt ggggctgcc gtcgtctgc gacgacgga 
      tagcaacga aacgggcgg aacaccaac ttcgattat 
      ttcgggatc gtcacctgt gacctcgcc ggcgttagg 
      gggacaacc ctacaccca ttccagtga tagttcaaa 
      tgaagctga taagaaaac cattttcaa agacgcgct 
      taaacctgt taaaccaac tgctgtccg agcgacgtc 
      cgccatgaa cagtagtac caactctat cctgagcta 
      tagttgcaa tagtacgac ctcaacacc tagcagcaa 
      accagttct agtaagtag tactcccag caagcaatc 
      ggctcgaga agcatcacc ggtggcgca attaaattc 
      gaccggaaa ctttgccac agttgctgc aacaacaac 
      agcgtcgaa agtgaccgc aactaatgg ggaacccgt 
      catgtcttc tgtgataac taacaataa tcaagttca 
      aacgcaaaa caatacggc cgtcgtaac gtcgtcgca 
      tactcaaaa ttcgacaaa tgcgcactc gacgcaaaa 
      ctcaaacac cgtatctca gaaattcaa cgaacaaca 
      tgtgcaaaa tagtagtat tagtcccga acttaataa 
      cagcagcag gtcctcgcc aatgactaa tacggaagt 
      acaacacat acagatgat gaacggacc aacgaccga 
      aagtaataa gattgcgga cggcatgtc gtctctgaa 
      gacgatggc gcagcaggt gatcgaccg cgcgggctt 
      ggagagcaa ccagcagca ggtgatttt agaagcgaa 
      caaaatcca taatcagga gaaatcgga cgcgcacat 
      ccccccgtt attgggggt ggcgccgct cggaacgat 
      accgctcca aaaagagca ccaggcgca gttccagct 
      catggaagg tgcctatta tcacatgcc acatccatc 
      cgattccga aaaagtaag aacttactt gccaaggaa 
      ctcatatcc aacaccacc ttattatat acaaacacc 
      attaccaca ttccgatac cgtggaatt ttatcagcg 
      tttggcgaa cgaaacatt attttttgt tttttatta 
      catggaggg cacaaaagc tcaatattt ggccgcgaa 
      agcgcttaa aaagcaaag ttggaggtt tcatacgaa 
      gtatatgat gtggttcca gaggcacga agagcccaa 
      aattattaa cgaagagta cgaacaggg aacttacat 
      ttacttcga ttatgaaaa gtggggcca acgaaaaaa 
      agaaggatt tacctttga atataagta cttagagga 
      tagagacct gaactgaat aaagtattt aaaaacagt 
      tgtgatttt tatttttgg ttgtgccgc ccagctgaa 
      gtcgataat tgcttgttt gtgtaaata aacaattaa 
      atgccacaa atgttccat gatgtagtt gagaaggta 
      ctgtagggg agtgcaaca cgttaaagg ttagtgtct 
      ttcaacttt tactatgta gaattaagg caaataaaa 
      gtgtcacac tgat