Status
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Gene information
General information
TC002496 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26517016..26538351
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002496
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection



    Pupal morphology
    head necrotic - Number of animals showing the phenotype: 8
    thorax necrotic - Number of animals showing the phenotype: 8
    abdomen necrotic - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002496 RA
      MAAVAAAQK NREMFAIKK SYSIENGYP ARRRSLVDD 
      ARFETLVVK QTKQSVLDE ARLRANDSS VDPEIDIED 
      KPAQKIDGE DDSGLTEEE VVLSNAIAE GPDAEKALQ 
      RAALILRLR EGMNSLSRV LKSIEKHQG AVCHLETRP 
      NQSDNNQLD ALVKVEMTR VSLLQLIKS LRQSSSLEH 
      CTLIGEDNI SAKNPWFPR HASDLDNCN HLMTKYEPD 
      LDMNHPGFA DKEYRARPD LDMNHPGFA DKEYRARRK 
      EIAEIAFAY KYGDPIPYI QYTETETKT WGSVFNTVL 
      ELMPKHACS EYCRVFKML QEEDIFTPD RIPQLEEMS 
      NFLKRHTGF SLRPAAGLL TARDFLASL AFRIFQSTQ 
      YVRHKNTPY HTPEPDCIH ELLGHMPLL ADPSFAQFS 
      QEIGLASLG ASDAEIEKL STVYWFTVE FGLCKESGV 
      VKAYGAGLL SAYGELLHA LSDKPELRP FEPAVTAVQ 
      PYQDQEYQP IYFVAESFD DMKDKFRRW VSAMSRPFE 
      VRFNPHTGR VEVLDSVEK LETLVHQLN TEVLHLSNA 
      INKMKAPSY Q
    CDS sequence
    • >TC002496 RA
      atggctgct gtggctgct gctcaaaaa aatcgcgaa 
      atgttcgcc atcaaaaag tcgtatagc atcgagaac 
      ggttatccc gctcgtcgg cgttccctt gttgacgac 
      gcaaggttc gagactttg gtggtgaaa caaaccaaa 
      caatcggtc ctggacgag gctcgcctt agggccaac 
      gattcaagc gttgatccc gaaattgac atcgaggac 
      aaaccggca cagaaaatc gacggcgaa gacgattct 
      ggcctgact gaggaggaa gtcgtcctt tctaatgcc 
      atcgccgaa gggccagac gctgaaaag gctctgcag 
      cgtgccgcc ctaatcctg cgtctacgc gaaggcatg 
      aactcgctc tccagagtg ctcaagagc atcgaaaaa 
      caccaaggg gcagtatgt cacctggag acgcgaccc 
      aaccagagc gacaacaac cagctggac gcgctcgtc 
      aaggtcgag atgaccaga gtcagtctc ttgcaactt 
      atcaagtcg ctgaggcag tccagttcg ttggaacac 
      tgcactttg attggagag gataatatt tcggcgaag 
      aatccctgg tttccaaga catgccagt gatttggac 
      aactgcaat catctgatg actaaatat gagcctgat 
      ttggatatg aaccatcct gggtttgct gataaggag 
      taccgggca cgtcctgat ttggatatg aaccatcct 
      gggtttgct gataaggag taccgggca cgtcgtaag 
      gaaatcgca gagattgct ttcgcctac aaatatgga 
      gaccccatt ccctacatc cagtacacg gaaaccgag 
      acgaagacc tggggctcc gtgttcaac accgtcctt 
      gagctgatg ccaaaacac gcttgcagc gagtactgc 
      cgcgtgttt aaaatgctc caggaagag gacattttt 
      acgccggat cgcattcca cagctggaa gaaatgtcg 
      aactttttg aaacgccac accggtttt tcgctgcgt 
      ccggccgcc ggcttgctc acagctcgt gatttcctc 
      gcttctctc gctttccgc atcttccag agcacccaa 
      tacgtccga cacaagaac accccgtac cacacgccc 
      gaaccggat tgcatccac gaacttttg ggccacatg 
      cccctgctg gcagacccc agcttcgct cagttctcg 
      caagagatt ggattggct tctcttggc gcctccgat 
      gctgaaatt gaaaaatta tcaacggtg tactggttc 
      accgtcgag ttcggcctc tgcaaagaa agcggcgtg 
      gtgaaagcc tacggcgcc ggcctcctc agcgcctac 
      ggcgagctc ctgcacgcc ctcagcgac aagccggag 
      ctgcggccc ttcgagccg gccgtcacc gccgtgcag 
      ccctaccag gaccaggag taccagccc atctacttc 
      gtcgccgag agcttcgac gacatgaag gacaagttc 
      cggcgatgg gtgtcggcc atgtcgcgc cccttcgaa 
      gtgagattc aacccgcac accggccgc gtggaggtg 
      ctcgactcc gtggagaag ctcgaaacg ctcgtccac 
      cagctcaac accgaagtc ctccacctg tcaaacgct 
      ataaacaaa atgaaagcg ccgtcgtat cagtga
    mRNA sequence
    • >TC002496 RA
      aggcgtgct tttcgcaac aatgaaagg gccggagcc 
      gcgtgggga cggcccgag gaggggctc acctacata 
      taacgttgc ccccagagc tccaaacac accttgctt 
      tgcttcaag tcaccagaa gcgtgtaat tgacagacg 
      gttccagtc acaatcgtg gtcgaattc acgcattca 
      aattacaca ctcgcgatt gccagcagt gaacaccta 
      gtgctttca ggctgattt tttttttgg ctcttcttc 
      gtacgctca agtctccgg ttgcaagac atggctgct 
      gtggctgct gctcaaaaa aatcgcgaa atgttcgcc 
      atcaaaaag tcgtatagc atcgagaac ggttatccc 
      gctcgtcgg cgttccctt gttgacgac gcaaggttc 
      gagactttg gtggtgaaa caaaccaaa caatcggtc 
      ctggacgag gctcgcctt agggccaac gattcaagc 
      gttgatccc gaaattgac atcgaggac aaaccggca 
      cagaaaatc gacggcgaa gacgattct ggcctgact 
      gaggaggaa gtcgtcctt tctaatgcc atcgccgaa 
      gggccagac gctgaaaag gctctgcag cgtgccgcc 
      ctaatcctg cgtctacgc gaaggcatg aactcgctc 
      tccagagtg ctcaagagc atcgaaaaa caccaaggg 
      gcagtatgt cacctggag acgcgaccc aaccagagc 
      gacaacaac cagctggac gcgctcgtc aaggtcgag 
      atgaccaga gtcagtctc ttgcaactt atcaagtcg 
      ctgaggcag tccagttcg ttggaacac tgcactttg 
      attggagag gataatatt tcggcgaag aatccctgg 
      tttccaaga catgccagt gatttggac aactgcaat 
      catctgatg actaaatat gagcctgat ttggatatg 
      aaccatcct gggtttgct gataaggag taccgggca 
      cgtcctgat ttggatatg aaccatcct gggtttgct 
      gataaggag taccgggca cgtcgtaag gaaatcgca 
      gagattgct ttcgcctac aaatatgga gaccccatt 
      ccctacatc cagtacacg gaaaccgag acgaagacc 
      tggggctcc gtgttcaac accgtcctt gagctgatg 
      ccaaaacac gcttgcagc gagtactgc cgcgtgttt 
      aaaatgctc caggaagag gacattttt acgccggat 
      cgcattcca cagctggaa gaaatgtcg aactttttg 
      aaacgccac accggtttt tcgctgcgt ccggccgcc 
      ggcttgctc acagctcgt gatttcctc gcttctctc 
      gctttccgc atcttccag agcacccaa tacgtccga 
      cacaagaac accccgtac cacacgccc gaaccggat 
      tgcatccac gaacttttg ggccacatg cccctgctg 
      gcagacccc agcttcgct cagttctcg caagagatt 
      ggattggct tctcttggc gcctccgat gctgaaatt 
      gaaaaatta tcaacggtg tactggttc accgtcgag 
      ttcggcctc tgcaaagaa agcggcgtg gtgaaagcc 
      tacggcgcc ggcctcctc agcgcctac ggcgagctc 
      ctgcacgcc ctcagcgac aagccggag ctgcggccc 
      ttcgagccg gccgtcacc gccgtgcag ccctaccag 
      gaccaggag taccagccc atctacttc gtcgccgag 
      agcttcgac gacatgaag gacaagttc cggcgatgg 
      gtgtcggcc atgtcgcgc cccttcgaa gtgagattc 
      aacccgcac accggccgc gtggaggtg ctcgactcc 
      gtggagaag ctcgaaacg ctcgtccac cagctcaac 
      accgaagtc ctccacctg tcaaacgct ataaacaaa 
      atgaaagcg ccgtcgtat cagtgaatg ccgggtagc 
      tgtgtgact ggtgctacc ctcgtgctt tcatcggct 
      cattgtata aatctgtca ttacctgta ttataacag 
      tctataatt attattgtt taatgtaca cgtagttta 
      gcgtagaca agtgatcaa gtagcttgt gatctcact 
      tcgaaactt atgcaccga ttagctgcg ataatttaa 
      ttttattta atttttcta gatatgctt agtgatatt 
      gaagcatgg taaagtatt tattttatt gtaaagttg 
      cacctgcac gatcgtaaa atatagaaa attatatgt 
      taagtaata tttgttgta acatataaa aatgctata 
      aaataaagt gtgcgataa tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY