Status
Please wait ...
Gene information
General information
TC002493 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26721592..26733843
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002493
    iBeetle screen iB_00388

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature fragmented - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen musculature partially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    procephalic head mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    gena triplet irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    vertex triplet irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002493 RA
      MADSEFEEF RHSFEKLTS HTKNSAPFY SLVHDVVVD 
      EESPTSSKS DPETDCDIV EESRLSGSS TSEKKEPSR 
      STPIPPWTG ERRRRKLPE IPKNKKPSV VSMYNAQYH 
      VSREISLAD ELGSTGGGG SGPSSILVL KCGYLWDTD 
      SPDSERLIT DADSGHSTA HSPTGTDGF KSMSPIMGI 
      SPSCVDGGL VYNDVEMLE ATHRGLHKF VPRHDDEIE 
      VEIGDPIYV QKEADDLWS EGVNLRTGR MGIFPSAYA 
      VDCDYSDWD SSCEHGRRE RYLLGYLGS VETLAHKGT 
      SVVCQAVRR VIGNSGSDP ESQPCILEV SDQGLRMVD 
      RRKRNQSEP CIDYFYSLK NVSFCAFHP RDHRYLGFI 
      TKHPTLQRF ACHVFRGTD STRPVAEAV GRAFQRFYQ 
      KFIETAYPV EDIYIE
    CDS sequence
    • >TC002493 RA
      atggctgat agtgagttt gaagaattt agacacagc 
      tttgaaaaa ctaacatcg cacaccaaa aattcagca 
      ccattttat tccttagtt catgatgtt gtcgtggat 
      gaagaatcg cccacgtct tccaagagc gaccccgaa 
      acagactgt gatattgtg gaggagagc agactctcg 
      ggctcctcc acaagcgaa aagaaagaa ccttcgagg 
      agtactccc attcctccc tggacggga gaacgcagg 
      aggagaaaa ctgcccgaa attccgaaa aataagaaa 
      ccttctgtc gtcagtatg tacaatgca caataccac 
      gtatctcga gaaatctct ctcgcagac gaactaggc 
      agtacaggg ggaggaggc agcggacca agctcaatc 
      ttagtatta aaatgcggc tatctatgg gacacagac 
      tcgcccgat tcggagcgc cttatcaca gacgccgac 
      agcggccac agcacggcc cattctccc acaggaacc 
      gacggtttc aaatccatg tcgcccatc atgggcata 
      tcgccttcg tgcgtcgac ggaggcctg gtgtacaac 
      gacgtcgaa atgctggaa gccacccat cggggactt 
      cacaagttt gttccccga cacgatgac gagatcgag 
      gtggaaatc ggggatcca atctatgtg cagaaggag 
      gcggacgat ctctggtcg gaaggggtt aatctgcgg 
      acggggagg atggggatt ttcccgtct gcttatgca 
      gtggactgt gattacagc gattgggat tcatcgtgt 
      gagcacggg aggagggag cggtatttg ttggggtat 
      ttggggtcg gtggagacg ttagcacac aaggggacg 
      tcggtggtt tgtcaagcc gtgaggagg gttatcgga 
      aattcggga agcgatccg gagtcgcaa ccgtgcatt 
      cttgaggtt tccgatcaa ggcctgagg atggtcgac 
      aggaggaaa cgaaaccaa agtgagccg tgcatagat 
      tacttctat tccctgaaa aacgtatcg ttctgcgct 
      tttcatcct cgagatcat cgctattta ggatttata 
      acaaaacac ccaactctt caacggttt gcatgtcat 
      gtctttcga ggaactgat tcgaccagg cctgtggct 
      gaagcagta gggcgtgcc ttccagagg ttctaccag 
      aagtttatt gagactgct tatccggtt gaagatatc 
      tatattgaa taa
    mRNA sequence
    • >TC002493 RA
      gggttgaaa ataaacatg gctgatagt gagtttgaa 
      gaatttaga cacagcttt gaaaaacta acatcgcac 
      accaaaaat tcagcacca ttttattcc ttagttcat 
      gatgttgtc gtggatgaa gaatcgccc acgtcttcc 
      aagagcgac cccgaaaca gactgtgat attgtggag 
      gagagcaga ctctcgggc tcctccaca agcgaaaag 
      aaagaacct tcgaggagt actcccatt cctccctgg 
      acgggagaa cgcaggagg agaaaactg cccgaaatt 
      ccgaaaaat aagaaacct tctgtcgtc agtatgtac 
      aatgcacaa taccacgta tctcgagaa atctctctc 
      gcagacgaa ctaggcagt acaggggga ggaggcagc 
      ggaccaagc tcaatctta gtattaaaa tgcggctat 
      ctatgggac acagactcg cccgattcg gagcgcctt 
      atcacagac gccgacagc ggccacagc acggcccat 
      tctcccaca ggaaccgac ggtttcaaa tccatgtcg 
      cccatcatg ggcatatcg ccttcgtgc gtcgacgga 
      ggcctggtg tacaacgac gtcgaaatg ctggaagcc 
      acccatcgg ggacttcac aagtttgtt ccccgacac 
      gatgacgag atcgaggtg gaaatcggg gatccaatc 
      tatgtgcag aaggaggcg gacgatctc tggtcggaa 
      ggggttaat ctgcggacg gggaggatg gggattttc 
      ccgtctgct tatgcagtg gactgtgat tacagcgat 
      tgggattca tcgtgtgag cacgggagg agggagcgg 
      tatttgttg gggtatttg gggtcggtg gagacgtta 
      gcacacaag gggacgtcg gtggtttgt caagccgtg 
      aggagggtt atcggaaat tcgggaagc gatccggag 
      tcgcaaccg tgcattctt gaggtttcc gatcaaggc 
      ctgaggatg gtcgacagg aggaaacga aaccaaagt 
      gagccgtgc atagattac ttctattcc ctgaaaaac 
      gtatcgttc tgcgctttt catcctcga gatcatcgc 
      tatttagga tttataaca aaacaccca actcttcaa 
      cggtttgca tgtcatgtc tttcgagga actgattcg 
      accaggcct gtggctgaa gcagtaggg cgtgccttc 
      cagaggttc taccagaag tttattgag actgcttat 
      ccggttgaa gatatctat attgaataa tttctgtag 
      tgcaataac atgtacata ctacataca tttaacgct 
      gtgtcgtca ataaaacat aataatttt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY