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Gene information
General information
TC002476 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27688992..27699172
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002476
    iBeetle screen iB_00385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    wings blistered - Number of animals showing the phenotype: 7

    elytra duplicated - Number of animals showing the phenotype: 7
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002476 RA
      MEDIFQWCR DGNALQVRV WLDDTEHDM NQGDDHGFS 
      PLHWAAKRG HTKIVEMLL LRGARVNAT NRGDDTPLH 
      LAAAHGHRE IVLMLLRQR ADVNFTNEH GNTPLHYAC 
      FWGYREIAE DLVHHDAKV SLANKYGDT PLDKARGNL 
      AKILHDMAV ESGQDLKKI IFKDQSWLG LKTRSRDAT 
      LSRHKGINI KELHQREEI ASTPSGVTY RGTWQKNDV 
      VAKILNIRE VTSRISRDF NEEFPKLRI FSHPNILPV 
      IGCCNSPPY LIVISQYMP LGSLYNVLH ESSGIVVDN 
      AQALRFAVD IARGMAYLH SLERIIPEY RLNSRHVII 
      EDDLTARIN MADAKFSFQ EKGRIYHPA WMSPEALQK 
      KITDRNWEA SDMWSFAIL LWELATREV PFADLNPME 
      AGMKIALEG LRIAIKPGI SPHLSKLIK ICMNEDPGK 
      RPKFDMIVP ILDKMQR
    CDS sequence
    • >TC002476 RA
      atggaggat attttccag tggtgcagg gacgggaat 
      gccctccag gtgcgggtt tggctcgat gacaccgag 
      cacgacatg aaccagggg gatgaccat gggttcagt 
      ccgctgcac tgggcggcg aaaaggggg cacacgaag 
      attgtggag atgctcttg ctcaggggg gcgagggtc 
      aatgccacc aatcggggg gacgatacg cccttgcat 
      ctggcggct gcacatggg cacagggag attgtgctt 
      atgttatta cgtcaaagg gccgacgta aatttcaca 
      aacgaacac ggcaacact cccttacac tacgcctgt 
      ttttggggc tacagagaa attgccgaa gatttggtc 
      catcatgac gccaaagtc tctcttgct aacaaatac 
      ggcgacact ccacttgac aaagcaaga ggcaatttg 
      gcgaaaatc ctccacgac atggcggtc gaatccggc 
      caagacctc aaaaagatc atcttcaaa gaccaaagt 
      tggctgggc ctgaaaacc cgctcacgt gacgccacc 
      ctttcccgt cacaaaggc atcaacatt aaagaatta 
      caccaacgt gaagaaatc gcctcaaca cccagtggc 
      gtaacatac agaggaaca tggcagaaa aatgacgtt 
      gttgctaaa atcttaaac attcgcgag gttacatcg 
      cgaatatcg cgcgatttt aacgaagaa ttcccgaaa 
      ttgcgaatt ttctcgcac cctaatatc ctcccagtc 
      attggctgt tgcaacagt ccgccatat ttgatcgtc 
      atcagtcaa tatatgcca ctcggatcg ctttacaat 
      gttttgcac gaaagcagc ggaattgtg gttgataat 
      gcgcaagcg ttgcgattt gctgttgat attgcaaga 
      ggcatggcc tacttgcat agtcttgag cgtattatt 
      ccagaatat cgtctgaat agtcgtcac gtgatcatt 
      gaggatgat ttgacggcg agaatcaac atggccgat 
      gctaaattc tccttccag gagaagggt aggatttat 
      cacccggcc tggatgtct cccgaagcg ctccagaaa 
      aaaatcacc gatcgcaac tgggaggcg tccgatatg 
      tggagtttt gcgattttg ttgtgggag ttggcaacg 
      agggaagtt cccttcgct gatttgaat cctatggag 
      gctgggatg aagatagct ttggaggga ttaaggatt 
      gccataaaa ccggggatt tccccacat ttgtccaag 
      ttgatcaag atttgtatg aatgaagat ccggggaaa 
      aggcccaag tttgatatg attgtccca attttggat 
      aaaatgcaa cgttga
    mRNA sequence
    • >TC002476 RA
      cccattcct tttatggta aatttttaa ttaatttgg 
      gggcccaat ctaactaat tcttaccta acggaacgg 
      gggacagtt tttaggacg ttacgtttg atttaaaac 
      cctttaaaa caataaaaa aatcaactt ttttatcag 
      tttggggaa tttcacgcg gcacggttg gcaacacgt 
      ccaaatatg gccgtctgt tgtgtaaaa aagtgttta 
      gttttcctc acctaatta ccaaaaaaa tccttatct 
      tatcaaagt aatgcggta aatagcttc cggataagc 
      gactgaacg cactcatta taaataaaa accaattcc 
      cgcgtttta aacaccacc caagtgaac gcccatttg 
      ctggtacaa gtgccccac ttttgggca aattgccga 
      aatggagga tattttcca gtggtgcag ggacgggaa 
      tgccctcca ggtgcgggt ttggctcga tgacaccga 
      gcacgacat gaaccaggg ggatgacca tgggttcag 
      tccgctgca ctgggcggc gaaaagggg gcacacgaa 
      gattgtgga gatgctctt gctcagggg ggcgagggt 
      caatgccac caatcgggg ggacgatac gcccttgca 
      tctggcggc tgcacatgg gcacaggga gattgtgct 
      tatgttatt acgtcaaag ggccgacgt aaatttcac 
      aaacgaaca cggcaacac tcccttaca ctacgcctg 
      tttttgggg ctacagaga aattgccga agatttggt 
      ccatcatga cgccaaagt ctctcttgc taacaaata 
      cggcgacac tccacttga caaagcaag aggcaattt 
      ggcgaaaat cctccacga catggcggt cgaatccgg 
      ccaagacct caaaaagat catcttcaa agaccaaag 
      ttggctggg cctgaaaac ccgctcacg tgacgccac 
      cctttcccg tcacaaagg catcaacat taaagaatt 
      acaccaacg tgaagaaat cgcctcaac acccagtgg 
      cgtaacata cagaggaac atggcagaa aaatgacgt 
      tgttgctaa aatcttaaa cattcgcga ggttacatc 
      gcgaatatc gcgcgattt taacgaaga attcccgaa 
      attgcgaat tttctcgca ccctaatat cctcccagt 
      cattggctg ttgcaacag tccgccata tttgatcgt 
      catcagtca atatatgcc actcggatc gctttacaa 
      tgttttgca cgaaagcag cggaattgt ggttgataa 
      tgcgcaagc gttgcgatt tgctgttga tattgcaag 
      aggcatggc ctacttgca tagtcttga gcgtattat 
      tccagaata tcgtctgaa tagtcgtca cgtgatcat 
      tgaggatga tttgacggc gagaatcaa catggccga 
      tgctaaatt ctccttcca ggagaaggg taggattta 
      tcacccggc ctggatgtc tcccgaagc gctccagaa 
      aaaaatcac cgatcgcaa ctgggaggc gtccgatat 
      gtggagttt tgcgatttt gttgtggga gttggcaac 
      gagggaagt tcccttcgc tgatttgaa tcctatgga 
      ggctgggat gaagatagc tttggaggg attaaggat 
      tgccataaa accggggat ttccccaca tttgtccaa 
      gttgatcaa gatttgtat gaatgaaga tccggggaa 
      aaggcccaa gtttgatat gattgtccc aattttgga 
      taaaatgca acgttgagc agttggctg ggtaaaatt 
      taaacgact aaaatgata gtgtgcctt ggatattgc 
      cttctatta attaataca ctgacattg tgttctaac 
      cacagcttt cttgcttaa cggagggaa aattttaaa 
      tttgaattt tgaatctct aaatggcgc ctccaataa 
      tcgagaaag atgtgtttt ctttttaat ttgtattta 
      atgttttta tttaatttt tgtatgtat tttatttag 
      atgtgctat actatacat aatttttta atgtattat 
      atttattaa taatttaat aactatgac atgttaata 
      cttttagga tattatatt ttaataata aaatgtaac 
      tgttgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY