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Gene information
General information
TC002472 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid26: 1281381..1312955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002472
    iBeetle screen iB_00384
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002472 RA
      MSTNPQWKS FQPPDTMNQ DSAIIDFKP MTSNGKASQ 
      AFNFTNRST SNSRFGQDF PLLQDKSSS FSNGLYGSS 
      STTHTNSRF PIGTFSLDS NNMFNSSGD STITSNNVF 
      SNGNNSSYN DSNSSNLGT RETGIIEKL LHSYGFIQC 
      CERQARLFF HFSQFSGNI EHLKIGDPV EFEMTYDRR 
      TGKPIASAV TKIAPEVVM SEERVTGTV TTELRGEGS 
      GGDSTGRIS YENRGECFF LPYTKDDVE GNVTLRAGD 
      KVSFQIATN NRGNLGACH VRLENPAHP VKYQGVVCS 
      MKESFGFIE RADVVKEIF FHFSEAKTK EELRLGDDV 
      EFTIQTRNG KEVACNITR LPSGTVIFE DLKPEILKG 
      QVLKPLDRG PNARHQTDA LPGRIRYRA PDHSEVEIS 
      FGDKDQRGD FTLRHGDWV QFKIATDRR DGLNRATHI 
      SLLDESFLV SGEKREQGV VASVKEGFG FLKCVEREA 
      RLFFHFNEI LDVDREVVV NDEYEFTVV QDQTSTFSN 
      NRQSAIRMK WLPHGSVQF ETRVENNLT GTVFKEIPT 
      TTWLNRSPT KNQNGEHAP ESGLITYQV NGVKKTLPF 
      YSKDCDMKH YPRLGDKVE FNVNQVKRN KELIAVDIQ 
      VVQPATQTN GIKHRTSNN CQIYQGFIA ALKDGFGFI 
      ETTQHDKEV FFHFSNFEG EPNSLELGQ EVEYNLGSR 
      GNSGTCSSA ENVKIIPKG TINLPPVIG DVLDGTVVR 
      PLRSVNPDQ TEYSGLIRI KTEDPEEKA LEYEFGIMG 
      LVNKRELLQ VGDHVQLQV DSTGRAANI VAVRKKLRA 
      TVDAIKGHF GFLAYEVEE GKKLFFHMT EVKDNVNLQ 
      VGDTVEFVL VTNHRSGKS SACSVVKVS DVQARPERL 
      ISRLRTVSV DDAGPKLTV LRQPKGPDG SKGFSSEAR 
      IVRLPGIVV E
    CDS sequence
    • >TC002472 RA
      atgtccacc aatcctcag tggaaatcc ttccagccg 
      cccgacacc atgaatcaa gactctgcc atcatcgat 
      ttcaaacca atgaccagc aacggcaaa gcatctcaa 
      gcatttaat tttacaaat cgctccaca tctaattca 
      cggttcggg caagacttt cctttgctg caggacaag 
      tcttcatca ttcagcaac ggtctgtac ggatcatca 
      agtaccaca catacaaat tctcggttt ccgattggt 
      acgttctcg ctcgattcg aataacatg ttcaacagc 
      agcggagac agcaccatc accagcaac aacgttttc 
      tccaacgga aacaacagc agctacaac gactccaat 
      tcgtcgaat ttgggcacc agagaaacg ggcatcatc 
      gaaaagctg ctgcattct tacggattc atccagtgc 
      tgcgaacga caggctcgt ctctttttc cattttagt 
      cagttcagt ggtaatatt gaacatttg aaaattggt 
      gatccggtg gaatttgaa atgacgtac gatagacga 
      accggcaaa ccgatagcg agcgccgtt acaaaaata 
      gcaccggaa gtagttatg agcgaagaa agggtaacc 
      ggaacggtt acaaccgaa cttcgaggt gaaggctcc 
      ggtggagat tcaacagga aggattagt tatgaaaat 
      cgaggtgaa tgtttcttt ctaccctac accaaagac 
      gacgtcgaa ggcaatgtt acgttgaga gctggcgat 
      aaagtcagc ttccaaata gcgacgaat aacaggggc 
      aacttgggg gcatgccac gtgcgtctc gagaaccca 
      gcccaccca gtaaaatac caaggcgtc gtatgttca 
      atgaaagaa agtttcggt ttcatcgaa cgcgccgac 
      gtcgtcaaa gagatcttc tttcacttt tccgaagcc 
      aagaccaaa gaggaatta cgcttaggc gacgacgtc 
      gaattcacc attcaaacg cgcaacggt aaagaagtc 
      gcctgtaac ataacccgt ctaccttcc ggcaccgtc 
      atcttcgag gacctcaaa ccggaaatc ctcaaaggc 
      caggtgctg aagccgcta gatcggggc cccaatgca 
      cgccaccaa acggacgcc ctcccgggg cgcatccgc 
      taccgggcc cccgaccac tccgaagtg gagatctcc 
      ttcggagac aaggaccaa cgtggggac ttcacgctg 
      cgtcatggt gattgggtg cagtttaaa atcgcaacg 
      gatcgtcga gatggactc aatagagcg acgcatatt 
      tcgcttttg gacgagagt tttctggtg tcgggcgag 
      aagcgggag caaggagtt gtggcatcg gtgaaggag 
      gggttcggg tttttgaaa tgtgtggaa cgtgaggct 
      cgattgttt ttccatttc aatgaaata ctggatgtg 
      gatagagaa gtggtggtg aatgacgag tacgagttt 
      actgtcgtt caggatcag accagtacg ttttcgaat 
      aatcgacaa agtgccatt agaatgaag tggttgccg 
      catggaagc gtgcagttt gagacgaga gttgagaat 
      aatttgacg gggactgtt tttaaggag ataccgacg 
      acgacgtgg ttgaatcga agtcccacg aagaatcag 
      aatggagaa catgctccc gaatcgggg ttgatcact 
      tatcaagtg aacggagtc aaaaagact ttaccattc 
      tattcgaaa gattgtgat atgaagcat tacccgagg 
      cttggagat aaggtcgaa tttaacgta aatcaggtg 
      aagagaaac aaagaatta atagcagtt gacattcaa 
      gtggtgcaa cccgctaca caaaccaac ggaattaaa 
      catagaact agcaacaat tgtcaaata taccaaggt 
      ttcatagca gcgttgaaa gacggtttt ggattcatc 
      gaaactact caacatgat aaagaagtc tttttccat 
      tttagtaat ttcgaaggc gagcccaac agtcttgaa 
      ctgggccaa gaagttgaa tacaatttg ggtagtcgc 
      ggcaattcg ggcacgtgt tcttcagcc gaaaacgtt 
      aaaattatt ccgaaaggt acgataaat ctaccgcct 
      gtcattggc gatgttttg gacggaaca gtagtccgt 
      cctttacgt tcggtaaat cccgatcaa acggaatac 
      tccggactt attcgaata aaaacggaa gatcccgaa 
      gagaaagca ctggaatat gagtttgga ataatggga 
      cttgtcaat aaacgcgaa ttgttacaa gtgggtgac 
      catgtacaa ttacaagtg gattcaaca ggcagggct 
      gccaacatt gttgctgta cgaaagaaa ctacgagca 
      acggtagat gcaatcaaa ggtcatttc ggttttttg 
      gcttatgaa gtggaagag ggcaaaaag ttgttcttc 
      cacatgacg gaagttaag gataatgtt aatttacaa 
      gttggcgat actgtcgag tttgtgctt gtgaccaat 
      cataggagt ggaaagtcg tcggcttgt agtgtggtg 
      aaagtcagc gatgtgcaa gcgcgtcca gagcggtta 
      atcagccgt ctgcgtacc gtgtctgtg gacgatgcg 
      ggccccaaa ttgacggtt ttgcgtcaa ccgaaaggc 
      cccgatggc tccaaaggt ttctcatcc gaggcacgt 
      atcgtacgt ctgccagga atcgtcgtc gaatag
    mRNA sequence
    • >TC002472 RA
      ctcatgttg acattcaac atggcggca tgtcgtcag 
      tgctgtgct gtgtcgtgg tgagttgaa atttttgta 
      aattttcgt atacttaac atcaaaact ggtcgaaaa 
      acttgtgta actaagaaa aacttcaaa attagcata 
      gaaaattta aagtaaacc agtatcagc gcaaccact 
      ggccttgac aaattatga gcataacag cacaatacg 
      cacccagca cgcattgaa gacatctga ggatttttt 
      tatttatat attctgtta taattaaat tttgagtga 
      ctgtggata gtaattttt atacctatt ggattgttt 
      ttctagtaa cattgtaat aacgatata ttattgtga 
      agtttgttg agcataatt gtacctttg tgacttggt 
      ttcaaagta ggagtgata attttatta gtacggtct 
      cggctgcca taattgact tttgtgggg tatttacat 
      ataccacta accattctg tgatattca ctacactta 
      agaaacgat aagaagtga aaagataaa tcattgtac 
      tgatagact atgtccacc aatcctcag tggaaatcc 
      ttccagccg cccgacacc atgaatcaa gactctgcc 
      atcatcgat ttcaaacca atgaccagc aacggcaaa 
      gcatctcaa gcatttaat tttacaaat cgctccaca 
      tctaattca cggttcggg caagacttt cctttgctg 
      caggacaag tcttcatca ttcagcaac ggtctgtac 
      ggatcatca agtaccaca catacaaat tctcggttt 
      ccgattggt acgttctcg ctcgattcg aataacatg 
      ttcaacagc agcggagac agcaccatc accagcaac 
      aacgttttc tccaacgga aacaacagc agctacaac 
      gactccaat tcgtcgaat ttgggcacc agagaaacg 
      ggcatcatc gaaaagctg ctgcattct tacggattc 
      atccagtgc tgcgaacga caggctcgt ctctttttc 
      cattttagt cagttcagt ggtaatatt gaacatttg 
      aaaattggt gatccggtg gaatttgaa atgacgtac 
      gatagacga accggcaaa ccgatagcg agcgccgtt 
      acaaaaata gcaccggaa gtagttatg agcgaagaa 
      agggtaacc ggaacggtt acaaccgaa cttcgaggt 
      gaaggctcc ggtggagat tcaacagga aggattagt 
      tatgaaaat cgaggtgaa tgtttcttt ctaccctac 
      accaaagac gacgtcgaa ggcaatgtt acgttgaga 
      gctggcgat aaagtcagc ttccaaata gcgacgaat 
      aacaggggc aacttgggg gcatgccac gtgcgtctc 
      gagaaccca gcccaccca gtaaaatac caaggcgtc 
      gtatgttca atgaaagaa agtttcggt ttcatcgaa 
      cgcgccgac gtcgtcaaa gagatcttc tttcacttt 
      tccgaagcc aagaccaaa gaggaatta cgcttaggc 
      gacgacgtc gaattcacc attcaaacg cgcaacggt 
      aaagaagtc gcctgtaac ataacccgt ctaccttcc 
      ggcaccgtc atcttcgag gacctcaaa ccggaaatc 
      ctcaaaggc caggtgctg aagccgcta gatcggggc 
      cccaatgca cgccaccaa acggacgcc ctcccgggg 
      cgcatccgc taccgggcc cccgaccac tccgaagtg 
      gagatctcc ttcggagac aaggaccaa cgtggggac 
      ttcacgctg cgtcatggt gattgggtg cagtttaaa 
      atcgcaacg gatcgtcga gatggactc aatagagcg 
      acgcatatt tcgcttttg gacgagagt tttctggtg 
      tcgggcgag aagcgggag caaggagtt gtggcatcg 
      gtgaaggag gggttcggg tttttgaaa tgtgtggaa 
      cgtgaggct cgattgttt ttccatttc aatgaaata 
      ctggatgtg gatagagaa gtggtggtg aatgacgag 
      tacgagttt actgtcgtt caggatcag accagtacg 
      ttttcgaat aatcgacaa agtgccatt agaatgaag 
      tggttgccg catggaagc gtgcagttt gagacgaga 
      gttgagaat aatttgacg gggactgtt tttaaggag 
      ataccgacg acgacgtgg ttgaatcga agtcccacg 
      aagaatcag aatggagaa catgctccc gaatcgggg 
      ttgatcact tatcaagtg aacggagtc aaaaagact 
      ttaccattc tattcgaaa gattgtgat atgaagcat 
      tacccgagg cttggagat aaggtcgaa tttaacgta 
      aatcaggtg aagagaaac aaagaatta atagcagtt 
      gacattcaa gtggtgcaa cccgctaca caaaccaac 
      ggaattaaa catagaact agcaacaat tgtcaaata 
      taccaaggt ttcatagca gcgttgaaa gacggtttt 
      ggattcatc gaaactact caacatgat aaagaagtc 
      tttttccat tttagtaat ttcgaaggc gagcccaac 
      agtcttgaa ctgggccaa gaagttgaa tacaatttg 
      ggtagtcgc ggcaattcg ggcacgtgt tcttcagcc 
      gaaaacgtt aaaattatt ccgaaaggt acgataaat 
      ctaccgcct gtcattggc gatgttttg gacggaaca 
      gtagtccgt cctttacgt tcggtaaat cccgatcaa 
      acggaatac tccggactt attcgaata aaaacggaa 
      gatcccgaa gagaaagca ctggaatat gagtttgga 
      ataatggga cttgtcaat aaacgcgaa ttgttacaa 
      gtgggtgac catgtacaa ttacaagtg gattcaaca 
      ggcagggct gccaacatt gttgctgta cgaaagaaa 
      ctacgagca acggtagat gcaatcaaa ggtcatttc 
      ggttttttg gcttatgaa gtggaagag ggcaaaaag 
      ttgttcttc cacatgacg gaagttaag gataatgtt 
      aatttacaa gttggcgat actgtcgag tttgtgctt 
      gtgaccaat cataggagt ggaaagtcg tcggcttgt 
      agtgtggtg aaagtcagc gatgtgcaa gcgcgtcca 
      gagcggtta atcagccgt ctgcgtacc gtgtctgtg 
      gacgatgcg ggccccaaa ttgacggtt ttgcgtcaa 
      ccgaaaggc cccgatggc tccaaaggt ttctcatcc 
      gaggcacgt atcgtacgt ctgccagga atcgtcgtc 
      gaatagcaa gtggttaag tcattctca ttcatgtca 
      tactaccgt agcaactaa cttactctt gtcttagca 
      gtggagttg gctaaaatg tctattacg tatggttat 
      taaattatt gacaaattg caaaattgt tatggtaaa 
      ctagggcaa ggaaaacct tttcggttg tattacaca 
      ccaatatta gtcgtagct atgttgaat tgaacaaag 
      tcgttcgca gttttactg tgcttaaaa cactgctta 
      caaaccaaa aaaaaatta aaaaaaaag ttgataatc 
      cattgacaa atatgaata caatgaatt tattattag 
      gatacagag ttaaggtat tagattatg atctagaga 
      agactgcat cgataagta aactctaca ttataagac 
      cttttgtaa cttttattt agactccat ttaggtgta 
      atgttagtt tgcagtctc tgcagtacc cgcaacatc 
      ccgtatttg taatggatc tacatagtt ggtgtgtct 
      cttctcgct tttcttctg tgataatca caagcctcg 
      ttatttact tatttacca aagtagttt cctcttgat 
      tatagtgcc taaattttg cgcaatttt tttcagttg 
      tttttttat tttttaaac acttgaatg tgacttttt 
      ttttacaaa tgttgatgt gggtactgt gatgcagtt 
      tttcaaatt aactacaat attaaatgt ggattatct 
      ttctatttg taaattatt gttttaatt tcaaaggaa 
      atatactaa gtaatacta ttaagacaa cgcttttta 
      ttaggcaca aatccttgc aattaattg caccacttg 
      ccatttttg caaaaggcg taaaaaccc tctcttctt 
      tctatacga atcaattag gagatgatt tcataaatc 
      gtttcgcta ttttcctta aaacaaagt ttttacaac 
      tttttcggt gttttatta gtaaccttt ttatttcct 
      tatttgatc tactaaaaa aacgttaaa cgctctttg 
      taattattg ttataattt cttcattaa aaggctttg 
      tccttcggt gccttcaat tttttaaat attgttctt 
      tagtgttca tacatattt gtaagcttc cctaacttt 
      ttcaaagtt gtatttttt tcttaaaac atcgagaaa 
      tatgaaaat ttaactaat tagtattta caccacagt 
      ccattttta tcatttttt gataatcat tgatagttt 
      taggttcac actttgaga ggaagaaaa cagtccatc 
      aaattaaat ttcataatt tttttttaa caaaatagt 
      gcctcaaaa gaacatgta acagaccga ataattacg 
      gtctctcca cgtttttcg cttttcatc ttgaatgct 
      ttttgtcct tatattgta cattacaca atgtacgca 
      agtattgag agttctaat aattaatta gtaacaga