Status
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Gene information
General information
TC002471 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27824115..27826655
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002471
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00383

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002471 RA
      MHPLTLLLL ALALHLANN ASLSIYEQQ KYKQFLEGQ 
      GLYSPNDDV VILTVHNFK TQVMNSPHA WFVEFYNSW 
      CGFCQRFAP SWKALSTDV KGWADLVQI AALDCSVDE 
      NTPICREYE IMAYPTLRY FHEGYQPGP QNLGVAVQK 
      GDDVGAHRR YLIERLVTE QRERRGAHY PNLLPYEHA 
      TLDNLFSGR GELIQYGFL VVEKFGGFI GAGVTLDLH 
      KTPVIVRYA YDNNTVLLN KLGISTLPV VVSVDRNLH 
      YQVTSAGSR DAVKSVIGQ FLQKQNIKL SDDTPKQEI 
      FTGKWLDVQ VPDMSSLIQ ERAKQALKE RIKSMGDVV 
      FQMDLETAL RYSLKHEIA TTKTIEGEK LQVLRAYLT 
      ILRKYFPFG HGGQLFLTE LAMKASGES VQGSELAEV 
      ISRAEAENS YVFSSPQQW LACRGSSPA FRGYPCGLW 
      KLFHFLTVN SAEHNVNNR RADPLEVLS VMHGYVKNF 
      FGCQDCSRH FQEMALKRE MRNVSSLDS SVMWLWMAH 
      NEVNKRLAG DQTEDPEYP KVQFPSKER CPTCRVNDN 
      WELLEVLKY IKHMYSGIN VRYIGSDTT LLHVGLDGG 
      KAATSGSSS VFRQLDMTR LSEKAVRVR PVG
    • >TC002471 RB
      MHPLTLLLL ALALHLANN ASLSIYEQQ KYKQFLEGQ 
      GLYSPNDDV VILTVHNFK TQVMNSPHA WFVEFYNSW 
      CGFCQRFAP SWKALSTDV KGWADLVQI AALDCSVDE 
      NTPICREYE IMAYPTLRY FHEGYQPGP QNLGVAVQK 
      GDDVGAHRR YLIERLVTE QRERRGAHY PNLLPYEHA 
      TLDNLFSGR GELIQYGFL VVEKFGGFI GAGVTLDLH 
      KTPVIVRYA YDNNTVLLN KLGISTLPV VVSVDRNLH 
      YQVTSAGSR DAVKSVIGQ FLQKQNIKL SDDTPKQEI 
      FTGKWLDVQ VPDMSSLIQ ERAKQALKE RIKSMGDVV 
      FQMDLETAL RYSLKHEIA TTKTIEGEK LQVLRAYLT 
      ILRKYFPFG HGGQLFLTE LAMKASGES VQGSELAEV 
      ISRAEAENS YVFSSPQQW LACRGSSPA FRGYPCGLW 
      KLFHFLTVN SAEHNVNNR RADPLEVLS VMHGYVKNF 
      FGCQDCSRH FQEMALKRE MRNVSSLDS SVMWLWMAH 
      NEVNKRLAG DQTEDPEYP KVQFPSKER CPTCRVNDN 
      WELLEVLKY IKHMYSGIN VRYIGSDTT LLHVGLDGG 
      KAATSGSSS VFRQLDMSM CFILYVVSF FLLAILIRI 
      FLKRGYRKK PYVYDLLGK V
    CDS sequence
    • >TC002471 RA
      atgcatcca ctaactctc ctgctgctc gccctcgcc 
      ctccacctc gccaacaac gcttcgctg tccatctac 
      gagcagcaa aaatacaag cagttcctc gagggccaa 
      ggcctgtac tcgcccaac gacgacgtc gtcatcctc 
      accgtgcac aacttcaag acccaggtg atgaacagc 
      ccccacgcc tggttcgtc gagttctac aacagctgg 
      tgcggcttc tgccagcgc ttcgccccc tcgtggaag 
      gccctctcc accgatgtg aagggctgg gccgacctg 
      gtgcaaatc gccgccttg gactgctcc gtggacgag 
      aacacaccc atttgccgt gagtacgag atcatggcc 
      taccccacc ttgcgctac ttccacgag gggtaccaa 
      ccaggcccc cagaacttg ggcgtcgcc gtgcagaag 
      ggggacgac gtgggggcg caccggcgc tacctcatc 
      gagcggctc gtgaccgag cagcgcgag cgccggggg 
      gcccactac cccaattta ctaccgtac gaacacgca 
      actcttgat aacttgttc agtgggagg ggggaactt 
      atccagtat ggatttttg gtggttgag aaatttggg 
      gggtttata ggggctggg gtcacactt gacttgcac 
      aagacccca gttatcgtc agatatgcg tacgataac 
      aatacagtt ttattaaac aagttgggt ataagtacc 
      cttccggtc gtagtgagt gttgaccgt aacttgcat 
      tatcaagtc acaagtgcc gggtcacgt gacgcggtc 
      aagtcagtg attggtcag tttttgcaa aaacaaaat 
      atcaaactg agtgatgat actcccaaa caggagata 
      ttcacaggg aagtggctt gatgtccaa gtgcctgat 
      atgtcgtcc ctgatccag gaacgcgca aagcaggcc 
      ttgaaagaa aggatcaag agtatggga gatgtggtg 
      ttccagatg gaccttgaa accgccctt cgctactcc 
      cttaagcac gaaatcgca accactaaa accatagaa 
      ggggagaag ttgcaagtg ttgcgcgcc tacttgact 
      atccttcgt aagtacttc ccctttggc cacgggggc 
      caattgttc ctaaccgag ttggccatg aaggcgtcg 
      ggcgagtcc gtccaaggg tcggaactc gccgaagtc 
      atatcgcgg gccgaggct gagaacagt tacgtgttc 
      tcgtccccc cagcaatgg ctggcgtgc cgcggtagt 
      tccccggcg ttccgggga tacccctgc gggttatgg 
      aagttgttc catttccta acggtgaac tccgccgag 
      cacaatgtg aacaaccgg cgtgcggac ccgctggaa 
      gtcctctcc gtgatgcac ggctacgtg aagaacttc 
      ttcgggtgt caggactgc agccggcac ttccaggag 
      atggcgctg aagcgggag atgcgcaac gtgtcgtcg 
      cttgactcg tcggtgatg tggttgtgg atggcgcac 
      aacgaggtg aacaagcgg ctggcgggg gaccagacg 
      gaggacccg gagtacccc aaggtgcag ttcccgtcg 
      aaggagcgg tgtcccacg tgccgggtc aacgacaac 
      tgggagttg ctggaggtg ttgaagtac atcaagcac 
      atgtacagc gggatcaac gtgaggtac atcggctcg 
      gacacgacg ctcctccac gtgggcttg gacgggggc 
      aaggcggcg acgagcggc tccagcagc gtgtttaga 
      cagttagat atgacgagg ctatcggaa aaagccgta 
      cgtgtacga cctgttggg taa
    • >TC002471 RB
      atgcatcca ctaactctc ctgctgctc gccctcgcc 
      ctccacctc gccaacaac gcttcgctg tccatctac 
      gagcagcaa aaatacaag cagttcctc gagggccaa 
      ggcctgtac tcgcccaac gacgacgtc gtcatcctc 
      accgtgcac aacttcaag acccaggtg atgaacagc 
      ccccacgcc tggttcgtc gagttctac aacagctgg 
      tgcggcttc tgccagcgc ttcgccccc tcgtggaag 
      gccctctcc accgatgtg aagggctgg gccgacctg 
      gtgcaaatc gccgccttg gactgctcc gtggacgag 
      aacacaccc atttgccgt gagtacgag atcatggcc 
      taccccacc ttgcgctac ttccacgag gggtaccaa 
      ccaggcccc cagaacttg ggcgtcgcc gtgcagaag 
      ggggacgac gtgggggcg caccggcgc tacctcatc 
      gagcggctc gtgaccgag cagcgcgag cgccggggg 
      gcccactac cccaattta ctaccgtac gaacacgca 
      actcttgat aacttgttc agtgggagg ggggaactt 
      atccagtat ggatttttg gtggttgag aaatttggg 
      gggtttata ggggctggg gtcacactt gacttgcac 
      aagacccca gttatcgtc agatatgcg tacgataac 
      aatacagtt ttattaaac aagttgggt ataagtacc 
      cttccggtc gtagtgagt gttgaccgt aacttgcat 
      tatcaagtc acaagtgcc gggtcacgt gacgcggtc 
      aagtcagtg attggtcag tttttgcaa aaacaaaat 
      atcaaactg agtgatgat actcccaaa caggagata 
      ttcacaggg aagtggctt gatgtccaa gtgcctgat 
      atgtcgtcc ctgatccag gaacgcgca aagcaggcc 
      ttgaaagaa aggatcaag agtatggga gatgtggtg 
      ttccagatg gaccttgaa accgccctt cgctactcc 
      cttaagcac gaaatcgca accactaaa accatagaa 
      ggggagaag ttgcaagtg ttgcgcgcc tacttgact 
      atccttcgt aagtacttc ccctttggc cacgggggc 
      caattgttc ctaaccgag ttggccatg aaggcgtcg 
      ggcgagtcc gtccaaggg tcggaactc gccgaagtc 
      atatcgcgg gccgaggct gagaacagt tacgtgttc 
      tcgtccccc cagcaatgg ctggcgtgc cgcggtagt 
      tccccggcg ttccgggga tacccctgc gggttatgg 
      aagttgttc catttccta acggtgaac tccgccgag 
      cacaatgtg aacaaccgg cgtgcggac ccgctggaa 
      gtcctctcc gtgatgcac ggctacgtg aagaacttc 
      ttcgggtgt caggactgc agccggcac ttccaggag 
      atggcgctg aagcgggag atgcgcaac gtgtcgtcg 
      cttgactcg tcggtgatg tggttgtgg atggcgcac 
      aacgaggtg aacaagcgg ctggcgggg gaccagacg 
      gaggacccg gagtacccc aaggtgcag ttcccgtcg 
      aaggagcgg tgtcccacg tgccgggtc aacgacaac 
      tgggagttg ctggaggtg ttgaagtac atcaagcac 
      atgtacagc gggatcaac gtgaggtac atcggctcg 
      gacacgacg ctcctccac gtgggcttg gacgggggc 
      aaggcggcg acgagcggc tccagcagc gtgtttaga 
      cagttagat atgagtatg tgttttatt ttatatgtg 
      gtttccttt ttcttacta gctatacta atacgtata 
      tttctaaag cgaggctat cggaaaaag ccgtacgtg 
      tacgacctg ttgggtaaa gtgtga
    mRNA sequence
    • >TC002471 RA
      acttcgcac ttttatcga aatccttat cgctccgtt 
      attataatt aaactccta atccttatt tgttccaaa 
      ggagagatt aggctcatt atcagctaa gcaatatcc 
      gttcaattc cacaccgaa catttttcc caaagtatg 
      gaaagtgca acagctgtg atcatctgt caaaattat 
      gacaattgt gtacgtaaa caaaaccag ttcattcac 
      tcccaacat ctaaaccac caccaaaac caataaaac 
      tgcaccgaa accgcatct tatcgctga gattccatc 
      agtgcattc gcgaacgtg tacggtgac gtgtgcgtc 
      cttaacctc aaaatgcat ccactaact ctcctgctg 
      ctcgccctc gccctccac ctcgccaac aacgcttcg 
      ctgtccatc tacgagcag caaaaatac aagcagttc 
      ctcgagggc caaggcctg tactcgccc aacgacgac 
      gtcgtcatc ctcaccgtg cacaacttc aagacccag 
      gtgatgaac agcccccac gcctggttc gtcgagttc 
      tacaacagc tggtgcggc ttctgccag cgcttcgcc 
      ccctcgtgg aaggccctc tccaccgat gtgaagggc 
      tgggccgac ctggtgcaa atcgccgcc ttggactgc 
      tccgtggac gagaacaca cccatttgc cgtgagtac 
      gagatcatg gcctacccc accttgcgc tacttccac 
      gaggggtac caaccaggc ccccagaac ttgggcgtc 
      gccgtgcag aagggggac gacgtgggg gcgcaccgg 
      cgctacctc atcgagcgg ctcgtgacc gagcagcgc 
      gagcgccgg ggggcccac taccccaat ttactaccg 
      tacgaacac gcaactctt gataacttg ttcagtggg 
      aggggggaa cttatccag tatggattt ttggtggtt 
      gagaaattt ggggggttt ataggggct ggggtcaca 
      cttgacttg cacaagacc ccagttatc gtcagatat 
      gcgtacgat aacaataca gttttatta aacaagttg 
      ggtataagt acccttccg gtcgtagtg agtgttgac 
      cgtaacttg cattatcaa gtcacaagt gccgggtca 
      cgtgacgcg gtcaagtca gtgattggt cagtttttg 
      caaaaacaa aatatcaaa ctgagtgat gatactccc 
      aaacaggag atattcaca gggaagtgg cttgatgtc 
      caagtgcct gatatgtcg tccctgatc caggaacgc 
      gcaaagcag gccttgaaa gaaaggatc aagagtatg 
      ggagatgtg gtgttccag atggacctt gaaaccgcc 
      cttcgctac tcccttaag cacgaaatc gcaaccact 
      aaaaccata gaaggggag aagttgcaa gtgttgcgc 
      gcctacttg actatcctt cgtaagtac ttccccttt 
      ggccacggg ggccaattg ttcctaacc gagttggcc 
      atgaaggcg tcgggcgag tccgtccaa gggtcggaa 
      ctcgccgaa gtcatatcg cgggccgag gctgagaac 
      agttacgtg ttctcgtcc ccccagcaa tggctggcg 
      tgccgcggt agttccccg gcgttccgg ggatacccc 
      tgcgggtta tggaagttg ttccatttc ctaacggtg 
      aactccgcc gagcacaat gtgaacaac cggcgtgcg 
      gacccgctg gaagtcctc tccgtgatg cacggctac 
      gtgaagaac ttcttcggg tgtcaggac tgcagccgg 
      cacttccag gagatggcg ctgaagcgg gagatgcgc 
      aacgtgtcg tcgcttgac tcgtcggtg atgtggttg 
      tggatggcg cacaacgag gtgaacaag cggctggcg 
      ggggaccag acggaggac ccggagtac cccaaggtg 
      cagttcccg tcgaaggag cggtgtccc acgtgccgg 
      gtcaacgac aactgggag ttgctggag gtgttgaag 
      tacatcaag cacatgtac agcgggatc aacgtgagg 
      tacatcggc tcggacacg acgctcctc cacgtgggc 
      ttggacggg ggcaaggcg gcgacgagc ggctccagc 
      agcgtgttt agacagtta gatatgacg aggctatcg 
      gaaaaagcc gtacgtgta cgacctgtt gggtaaagt 
      gtgattgat ggtggaggg ctccggatg gagattgac 
      aacgaacaa ttgtgattt tataggttt aagttacat 
      cacattctt ttgtttacc aaagaagtc ggctttgtt 
      atgtaaata ttttagttg tttagtcga ttgcatttt 
      attaagtgg ttaggtaca agatcaagg ttgttttat 
      ctttatgtg tatttttgg tccaagacg ttactgatt 
      acgtagttt aaattatgc cgtttttta ttgtattta 
      taatgtttt atatccctt gattgaaag atatgtatg 
      taaataata aaatacaat tacagtt
    • >TC002471 RB
      acttcgcac ttttatcga aatccttat cgctccgtt 
      attataatt aaactccta atccttatt tgttccaaa 
      ggagagatt aggctcatt atcagctaa gcaatatcc 
      gttcaattc cacaccgaa catttttcc caaagtatg 
      gaaagtgca acagctgtg atcatctgt caaaattat 
      gacaattgt gtacgtaaa caaaaccag ttcattcac 
      tcccaacat ctaaaccac caccaaaac caataaaac 
      tgcaccgaa accgcatct tatcgctga gattccatc 
      agtgcattc gcgaacgtg tacggtgac gtgtgcgtc 
      cttaacctc aaaatgcat ccactaact ctcctgctg 
      ctcgccctc gccctccac ctcgccaac aacgcttcg 
      ctgtccatc tacgagcag caaaaatac aagcagttc 
      ctcgagggc caaggcctg tactcgccc aacgacgac 
      gtcgtcatc ctcaccgtg cacaacttc aagacccag 
      gtgatgaac agcccccac gcctggttc gtcgagttc 
      tacaacagc tggtgcggc ttctgccag cgcttcgcc 
      ccctcgtgg aaggccctc tccaccgat gtgaagggc 
      tgggccgac ctggtgcaa atcgccgcc ttggactgc 
      tccgtggac gagaacaca cccatttgc cgtgagtac 
      gagatcatg gcctacccc accttgcgc tacttccac 
      gaggggtac caaccaggc ccccagaac ttgggcgtc 
      gccgtgcag aagggggac gacgtgggg gcgcaccgg 
      cgctacctc atcgagcgg ctcgtgacc gagcagcgc 
      gagcgccgg ggggcccac taccccaat ttactaccg 
      tacgaacac gcaactctt gataacttg ttcagtggg 
      aggggggaa cttatccag tatggattt ttggtggtt 
      gagaaattt ggggggttt ataggggct ggggtcaca 
      cttgacttg cacaagacc ccagttatc gtcagatat 
      gcgtacgat aacaataca gttttatta aacaagttg 
      ggtataagt acccttccg gtcgtagtg agtgttgac 
      cgtaacttg cattatcaa gtcacaagt gccgggtca 
      cgtgacgcg gtcaagtca gtgattggt cagtttttg 
      caaaaacaa aatatcaaa ctgagtgat gatactccc 
      aaacaggag atattcaca gggaagtgg cttgatgtc 
      caagtgcct gatatgtcg tccctgatc caggaacgc 
      gcaaagcag gccttgaaa gaaaggatc aagagtatg 
      ggagatgtg gtgttccag atggacctt gaaaccgcc 
      cttcgctac tcccttaag cacgaaatc gcaaccact 
      aaaaccata gaaggggag aagttgcaa gtgttgcgc 
      gcctacttg actatcctt cgtaagtac ttccccttt 
      ggccacggg ggccaattg ttcctaacc gagttggcc 
      atgaaggcg tcgggcgag tccgtccaa gggtcggaa 
      ctcgccgaa gtcatatcg cgggccgag gctgagaac 
      agttacgtg ttctcgtcc ccccagcaa tggctggcg 
      tgccgcggt agttccccg gcgttccgg ggatacccc 
      tgcgggtta tggaagttg ttccatttc ctaacggtg 
      aactccgcc gagcacaat gtgaacaac cggcgtgcg 
      gacccgctg gaagtcctc tccgtgatg cacggctac 
      gtgaagaac ttcttcggg tgtcaggac tgcagccgg 
      cacttccag gagatggcg ctgaagcgg gagatgcgc 
      aacgtgtcg tcgcttgac tcgtcggtg atgtggttg 
      tggatggcg cacaacgag gtgaacaag cggctggcg 
      ggggaccag acggaggac ccggagtac cccaaggtg 
      cagttcccg tcgaaggag cggtgtccc acgtgccgg 
      gtcaacgac aactgggag ttgctggag gtgttgaag 
      tacatcaag cacatgtac agcgggatc aacgtgagg 
      tacatcggc tcggacacg acgctcctc cacgtgggc 
      ttggacggg ggcaaggcg gcgacgagc ggctccagc 
      agcgtgttt agacagtta gatatgagt atgtgtttt 
      attttatat gtggtttcc tttttctta ctagctata 
      ctaatacgt atatttcta aagcgaggc tatcggaaa 
      aagccgtac gtgtacgac ctgttgggt aaagtgtga 
      ttgatggtg gagggctcc ggatggaga ttgacaacg 
      aacaattgt gattttata ggtttaagt tacatcaca 
      ttcttttgt ttaccaaag aagtcggct ttgttatgt 
      aaatatttt agttgttta gtcgattgc attttatta 
      agtggttag gtacaagat caaggttgt tttatcttt 
      atgtgtatt tttggtcca agacgttac tgattacgt 
      agtttaaat tatgccgtt ttttattgt atttataat 
      gttttatat cccttgatt gaaagatat gtatgtaaa 
      taataaaat acaattaca gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY