General information | |||
TC002468 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 28025818..28030800 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC002468 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00382 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
remarks - cuticles: eggs lost,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC002468 RA
MGDWENLPV ELLAHLYSY LSRRDRLSC SLVCENWKS GLDHVILWK TVIVHVDSD LMEPSTVIL LRDYCKYIK NLEFGWAVP HRNNKWAQV RYKELTKRA VRFFLVLYD NYIQIHSLR IFNWFDFMS FQKVTYHLS RFLKNQVSL KKIIFDNIN MHSANYMKV LTSCLGSRG TITHLDIRS CYYCQSSFD AIYFRSCIE QLTYLKSFK LDYFILSCR LIDSILTSK NRSLEHLEI VLKETSWHD HVIDDEQWC LLCSACPNL KVTLIIRNV YHYGELRLY LKQSIPIVS LSIILPRVM DRGGRWNFG KTLNLLVSN YHRTLETID LHIKNDGEV VDDVLLRLI HRCSKLKSF LFNGYLENP QLLKSSFSD ITFFCMTPN SLPDKHLVF NKVIRDDVT ELRHKRVQF TLIKRSPKS ICSLLVLN
- >TC002468 RA
atgggagat tgggaaaac ttgcctgtg gagttgttg gctcatttg tactcttac ctgtctaga agagaccga ctctcctgc tccctggtc tgcgagaac tggaaaagt ggtctcgat catgtgatt ttgtggaag actgtgatc gtccatgtg gattcggac ttgatggaa cccagtacc gtgattttg ttaagggat tattgtaaa tatatcaaa aatctggag tttgggtgg gccgtccct cacagaaac aacaaatgg gcacaagtt aggtataag gaactgacc aagagagct gtgcggttt tttctcgtt ttgtacgac aactacatc caaatccac agtttgagg atttttaac tggttcgac tttatgtcc ttccaaaaa gttacgtac catttatcg agattcctc aaaaaccaa gtgagtctc aaaaaaatc atctttgac aatatcaac atgcacagt gcgaactac atgaaagta ctaacttct tgtttgggc tctagaggc actataacg cacttagac atccgcagt tgttactac tgccagtct tcattcgat gcgatctat ttcagaagt tgtatcgaa caactcact tacctgaaa agcttcaaa cttgattat ttcatcctg tcgtgcaga ttaattgac tcgattttg accagtaag aacagatct ttggaacat ttggaaatt gttttgaaa gaaacatca tggcatgac cacgttata gacgatgaa cagtggtgt ctactgtgc agtgcatgc cccaatctc aaggtcact ctaatcatt cgaaatgtg taccattac ggtgaattg cgcctctac ctcaaacaa tcgatcccg atcgtgtcc ctctctatt attctacca cgagttatg gacagagga ggcagatgg aatttcgga aaaacgcta aatttacta gtgagcaac taccaccga actcttgaa accatcgat ttgcatatc aaaaacgat ggagaagtt gtagacgac gtccttctg cgactgatc caccgctgt tcaaaattg aaaagtttc ctctttaac ggctacttg gaaaacccc caacttctc aaaagctcg ttcagcgat atcaccttc ttctgcatg accccgaac tccctcccc gacaaacac ctcgttttc aacaaagtc attcgagac gatgtcact gaactgaga cacaaacgg gtgcagttc accctaatc aagcgtagc cctaagtca atttgttcg ctacttgtc ttaaattaa
- >TC002468 RA
acaaataaa attaacgta ggattaagt gagccccca tagctctaa gtagatata agcagagga tgccagacc ctattcgat ggtccggac acgatggga gattgggaa aacttgcct gtggagttg ttggctcat ttgtactct tacctgtct agaagagac cgactctcc tgctccctg gtctgcgag aactggaaa agtggtctc gatcatgtg attttgtgg aagactgtg atcgtccat gtggattcg gacttgatg gaacccagt accgtgatt ttgttaagg gattattgt aaatatatc aaaaatctg gagtttggg tgggccgtc cctcacaga aacaacaaa tgggcacaa gttaggtat aaggaactg accaagaga gctgtgcgg ttttttctc gttttgtac gacaactac atccaaatc cacagtttg aggattttt aactggttc gactttatg tccttccaa aaagttacg taccattta tcgagattc ctcaaaaac caagtgagt ctcaaaaaa atcatcttt gacaatatc aacatgcac agtgcgaac tacatgaaa gtactaact tcttgtttg ggctctaga ggcactata acgcactta gacatccgc agttgttac tactgccag tcttcattc gatgcgatc tatttcaga agttgtatc gaacaactc acttacctg aaaagcttc aaacttgat tatttcatc ctgtcgtgc agattaatt gactcgatt ttgaccagt aagaacaga tctttggaa catttggaa attgttttg aaagaaaca tcatggcat gaccacgtt atagacgat gaacagtgg tgtctactg tgcagtgca tgccccaat ctcaaggtc actctaatc attcgaaat gtgtaccat tacggtgaa ttgcgcctc tacctcaaa caatcgatc ccgatcgtg tccctctct attattcta ccacgagtt atggacaga ggaggcaga tggaatttc ggaaaaacg ctaaattta ctagtgagc aactaccac cgaactctt gaaaccatc gatttgcat atcaaaaac gatggagaa gttgtagac gacgtcctt ctgcgactg atccaccgc tgttcaaaa ttgaaaagt ttcctcttt aacggctac ttggaaaac ccccaactt ctcaaaagc tcgttcagc gatatcacc ttcttctgc atgaccccg aactccctc cccgacaaa cacctcgtt ttcaacaaa gtcattcga gacgatgtc actgaactg agacacaaa cgggtgcag ttcacccta atcaagcgt agccctaag tcaatttgt tcgctactt gtcttaaat taaagtgaa gcccaaatt ttttattta agtcggacc tgtacagct tttacaatg catattcaa ggcccccct tgaggtgaa gcccgaact acaaaactg gcacacgta ggggcgttc gccggtgtg ccccctcaa gtgaatacg taagggggc cccctgact gaagcattt cccgcagta ttcacacga gtagggctt ttccccact gtgtaccct ctcatgcat caccaaata atccttctt ggtgaaact tttagcaca gtagctgca agtgtaagg tttgacgcc tgtgtggac tctgtagtg aactctaag tttgttttt ctgggcgaa acctttgcc acataagtc acacaagtg ttttttctc atgtggaag cttcacttc gacgtgttt gtcttggaa atgcttctg gagtgtctc aactttgct gaaaacttt gctacagac gctacattg attggggag gaggcggcc ttcaattcc aactcgatg gcacctcac ttggtgtgt taggaggta cctggagta ggcaaaatc cttgttgca cacaataca aataaaggg acgcaaccc caagtgctc attattgtg ttctttgta caaaaccac atcgttaaa acctttacc acacgtagt acaaacgta gtaaaattt cctcaagtg gacgtagtt tatatgaat caaaagact cttgcgttt ggctgtctt gtacgtaca ttgaggaca cctgaagag cttgtcctt gctatgtat gaccatgtg ggtgccata actgtacgc tttagtgaa tctctgttg gcaaattgt acactcgaa aaattcctc gtgtccttt gacatgttt ctcgatttc ttcgcgttc ttggtactc ggctgagca agtgttgca aatgtagat ttcctgtat ttcgtcgaa tttgtaagt gtgtttttc gaacgagtc gtcagtggt gtgtgtttt gcgatgctt gcgcaacag gaggattgt tttgaactc ttgggagca tttgttgca agaccaggt tttgttgga attatatga gcgcttgcg acgagtgca agtctcgtc ttcgcttag gtcgctagg ggtataggt ggtgtcgat ggttttgcg tttgcggcg taagcgtct gaaagagaa atgggttat ttagggcga aaaagctat tttaatatg cttgccgtc aggctattt aaatgtcag ttattacag aaaatcgct tattttaca aatgctacc aaaaatttg agaacagtg gatgcaata gagtggccg ttctagtgt gttgacttt aataaaact gtaattaat aa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
