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Gene information
General information
TC002468 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid27: 188262..193244
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002468
      iBeetle screen iB_00382
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      remarks - cuticles: eggs lost,
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002468 RA
        MGDWENLPV ELLAHLYSY LSRRDRLSC SLVCENWKS 
        GLDHVILWK TVIVHVDSD LMEPSTVIL LRDYCKYIK 
        NLEFGWAVP HRNNKWAQV RYKELTKRA VRFFLVLYD 
        NYIQIHSLR IFNWFDFMS FQKVTYHLS RFLKNQVSL 
        KKIIFDNIN MHSANYMKV LTSCLGSRG TITHLDIRS 
        CYYCQSSFD AIYFRSCIE QLTYLKSFK LDYFILSCR 
        LIDSILTSK NRSLEHLEI VLKETSWHD HVIDDEQWC 
        LLCSACPNL KVTLIIRNV YHYGELRLY LKQSIPIVS 
        LSIILPRVM DRGGRWNFG KTLNLLVSN YHRTLETID 
        LHIKNDGEV VDDVLLRLI HRCSKLKSF LFNGYLENP 
        QLLKSSFSD ITFFCMTPN SLPDKHLVF NKVIRDDVT 
        ELRHKRVQF TLIKRSPKS ICSLLVLN
      CDS sequence
      • >TC002468 RA
        atgggagat tgggaaaac ttgcctgtg gagttgttg 
        gctcatttg tactcttac ctgtctaga agagaccga 
        ctctcctgc tccctggtc tgcgagaac tggaaaagt 
        ggtctcgat catgtgatt ttgtggaag actgtgatc 
        gtccatgtg gattcggac ttgatggaa cccagtacc 
        gtgattttg ttaagggat tattgtaaa tatatcaaa 
        aatctggag tttgggtgg gccgtccct cacagaaac 
        aacaaatgg gcacaagtt aggtataag gaactgacc 
        aagagagct gtgcggttt tttctcgtt ttgtacgac 
        aactacatc caaatccac agtttgagg atttttaac 
        tggttcgac tttatgtcc ttccaaaaa gttacgtac 
        catttatcg agattcctc aaaaaccaa gtgagtctc 
        aaaaaaatc atctttgac aatatcaac atgcacagt 
        gcgaactac atgaaagta ctaacttct tgtttgggc 
        tctagaggc actataacg cacttagac atccgcagt 
        tgttactac tgccagtct tcattcgat gcgatctat 
        ttcagaagt tgtatcgaa caactcact tacctgaaa 
        agcttcaaa cttgattat ttcatcctg tcgtgcaga 
        ttaattgac tcgattttg accagtaag aacagatct 
        ttggaacat ttggaaatt gttttgaaa gaaacatca 
        tggcatgac cacgttata gacgatgaa cagtggtgt 
        ctactgtgc agtgcatgc cccaatctc aaggtcact 
        ctaatcatt cgaaatgtg taccattac ggtgaattg 
        cgcctctac ctcaaacaa tcgatcccg atcgtgtcc 
        ctctctatt attctacca cgagttatg gacagagga 
        ggcagatgg aatttcgga aaaacgcta aatttacta 
        gtgagcaac taccaccga actcttgaa accatcgat 
        ttgcatatc aaaaacgat ggagaagtt gtagacgac 
        gtccttctg cgactgatc caccgctgt tcaaaattg 
        aaaagtttc ctctttaac ggctacttg gaaaacccc 
        caacttctc aaaagctcg ttcagcgat atcaccttc 
        ttctgcatg accccgaac tccctcccc gacaaacac 
        ctcgttttc aacaaagtc attcgagac gatgtcact 
        gaactgaga cacaaacgg gtgcagttc accctaatc 
        aagcgtagc cctaagtca atttgttcg ctacttgtc 
        ttaaattaa
      mRNA sequence
      • >TC002468 RA
        acaaataaa attaacgta ggattaagt gagccccca 
        tagctctaa gtagatata agcagagga tgccagacc 
        ctattcgat ggtccggac acgatggga gattgggaa 
        aacttgcct gtggagttg ttggctcat ttgtactct 
        tacctgtct agaagagac cgactctcc tgctccctg 
        gtctgcgag aactggaaa agtggtctc gatcatgtg 
        attttgtgg aagactgtg atcgtccat gtggattcg 
        gacttgatg gaacccagt accgtgatt ttgttaagg 
        gattattgt aaatatatc aaaaatctg gagtttggg 
        tgggccgtc cctcacaga aacaacaaa tgggcacaa 
        gttaggtat aaggaactg accaagaga gctgtgcgg 
        ttttttctc gttttgtac gacaactac atccaaatc 
        cacagtttg aggattttt aactggttc gactttatg 
        tccttccaa aaagttacg taccattta tcgagattc 
        ctcaaaaac caagtgagt ctcaaaaaa atcatcttt 
        gacaatatc aacatgcac agtgcgaac tacatgaaa 
        gtactaact tcttgtttg ggctctaga ggcactata 
        acgcactta gacatccgc agttgttac tactgccag 
        tcttcattc gatgcgatc tatttcaga agttgtatc 
        gaacaactc acttacctg aaaagcttc aaacttgat 
        tatttcatc ctgtcgtgc agattaatt gactcgatt 
        ttgaccagt aagaacaga tctttggaa catttggaa 
        attgttttg aaagaaaca tcatggcat gaccacgtt 
        atagacgat gaacagtgg tgtctactg tgcagtgca 
        tgccccaat ctcaaggtc actctaatc attcgaaat 
        gtgtaccat tacggtgaa ttgcgcctc tacctcaaa 
        caatcgatc ccgatcgtg tccctctct attattcta 
        ccacgagtt atggacaga ggaggcaga tggaatttc 
        ggaaaaacg ctaaattta ctagtgagc aactaccac 
        cgaactctt gaaaccatc gatttgcat atcaaaaac 
        gatggagaa gttgtagac gacgtcctt ctgcgactg 
        atccaccgc tgttcaaaa ttgaaaagt ttcctcttt 
        aacggctac ttggaaaac ccccaactt ctcaaaagc 
        tcgttcagc gatatcacc ttcttctgc atgaccccg 
        aactccctc cccgacaaa cacctcgtt ttcaacaaa 
        gtcattcga gacgatgtc actgaactg agacacaaa 
        cgggtgcag ttcacccta atcaagcgt agccctaag 
        tcaatttgt tcgctactt gtcttaaat taaagtgaa 
        gcccaaatt ttttattta agtcggacc tgtacagct 
        tttacaatg catattcaa ggcccccct tgaggtgaa 
        gcccgaact acaaaactg gcacacgta ggggcgttc 
        gccggtgtg ccccctcaa gtgaatacg taagggggc 
        cccctgact gaagcattt cccgcagta ttcacacga 
        gtagggctt ttccccact gtgtaccct ctcatgcat 
        caccaaata atccttctt ggtgaaact tttagcaca 
        gtagctgca agtgtaagg tttgacgcc tgtgtggac 
        tctgtagtg aactctaag tttgttttt ctgggcgaa 
        acctttgcc acataagtc acacaagtg ttttttctc 
        atgtggaag cttcacttc gacgtgttt gtcttggaa 
        atgcttctg gagtgtctc aactttgct gaaaacttt 
        gctacagac gctacattg attggggag gaggcggcc 
        ttcaattcc aactcgatg gcacctcac ttggtgtgt 
        taggaggta cctggagta ggcaaaatc cttgttgca 
        cacaataca aataaaggg acgcaaccc caagtgctc 
        attattgtg ttctttgta caaaaccac atcgttaaa 
        acctttacc acacgtagt acaaacgta gtaaaattt 
        cctcaagtg gacgtagtt tatatgaat caaaagact 
        cttgcgttt ggctgtctt gtacgtaca ttgaggaca 
        cctgaagag cttgtcctt gctatgtat gaccatgtg 
        ggtgccata actgtacgc tttagtgaa tctctgttg 
        gcaaattgt acactcgaa aaattcctc gtgtccttt 
        gacatgttt ctcgatttc ttcgcgttc ttggtactc 
        ggctgagca agtgttgca aatgtagat ttcctgtat 
        ttcgtcgaa tttgtaagt gtgtttttc gaacgagtc 
        gtcagtggt gtgtgtttt gcgatgctt gcgcaacag 
        gaggattgt tttgaactc ttgggagca tttgttgca 
        agaccaggt tttgttgga attatatga gcgcttgcg 
        acgagtgca agtctcgtc ttcgcttag gtcgctagg 
        ggtataggt ggtgtcgat ggttttgcg tttgcggcg 
        taagcgtct gaaagagaa atgggttat ttagggcga 
        aaaagctat tttaatatg cttgccgtc aggctattt 
        aaatgtcag ttattacag aaaatcgct tattttaca 
        aatgctacc aaaaatttg agaacagtg gatgcaata 
        gagtggccg ttctagtgt gttgacttt aataaaact 
        gtaattaat aa