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Gene information
General information
TC002467 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid27: 193309..197984
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002467
    iBeetle screen iB_00381
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002467 RA
      MLLKLLFLV LLGQIRAKP VEDEPVCGY EACPATNFS 
      TLNVHLIPH SHDDVGWLK TMDQYYFQD VQNVISTVI 
      PALKQNPDR RFVQVETAF FKKWWEQQK DSVKQDVIK 
      LVNNGQFEI INGAWSMND EANVHYQFT IDQYTLGLR 
      YLEDRLGKC SRPKVCWQI DPFGHSREQ ASISAQLGF 
      DSVFFARLD YRDRINRLG TKTGDLIWR GSSNLGNSS 
      DIFTSVMYN HYSAPAGFC FDIVCNDEP IIDDEESPD 
      YNWKSRVEA FANFVREQA SKYPTNNIL VPMGDDFRY 
      QAALNAYIN TDRLIKGFE LFPQTFEGK PIKLLYSTP 
      TCYTKAVND FVNSNNYKL QIKSDDFFP YADGVASLV 
      VGYFTSRPA SKRFVREAN TMLQVAKQL AAVANLPYE 
      NENINSLKE AMGVLQHHD AITGTELID VTHDYHRIL 
      HKGISDAVN AINPILSNL ISGSDSLKL ESCLLANVS 
      VCLQAKADQ FILTVYNPL SRVVSSPIS IPVETENWK 
      ILDSNGDEV VYQVVPSIT DFSTLVDET TSPNSLDFI 
      AKDIPALGY KVYTFTKVA TKPTKRITV GADGTTFEI 
      DEVSGLLTS VTMNGVTLN VTQDFLVYK SGGRSQAYV 
      FDPDGDPQR VASTVTVKK IEGDVYYGV VQEFSDWAK 
      QIIKVYKND SSYIEFDWV IGPLNIDDG GKEVISQFS 
      TPLQTEGVF YTDSNGREI LKRVRNQRP DYDYTDEQP 
      IAGNYYPVT SKILIRDEN LGVEMAILN DRAQGGSSV 
      KDGQVELMV HRRIRSGDG FGLDEIEYG HGIVVRGKH 
      FLVLGPVSG NGEKSLAAI ERDVAQRKL LPPWVFITN 
      QDVSGSKNL EYSSLKRPL PDNVQLLTL EPWSDGTLL 
      VRLEHVLEK NEDENLSKE VTVDLSDLF VAFTVQELK 
      ETTLAGNIP IEENVRLHW PGSTNEAPP QAPRAPTDL 
      TITLAPMQI RTFLATVTY NNL
    CDS sequence
    • >TC002467 RA
      atgttacta aaacttcta tttttggta ctcttgggc 
      caaattagg gcaaagccg gtcgaagac gagccagtt 
      tgtggatat gaggcttgc cctgcgacc aacttctcc 
      acactcaac gtccacctc atccctcac tcccatgac 
      gacgtcggt tggctcaaa accatggac cagtactac 
      ttccaagac gtccagaac gtgatcagt accgtaatc 
      ccagctttg aagcaaaac cctgaccgt cgtttcgtc 
      caagtcgaa acagcgttt tttaaaaaa tggtgggaa 
      cagcaaaag gactcagtc aagcaagat gttatcaaa 
      ctggtcaat aacgggcag ttcgagata attaacggc 
      gcctggagc atgaatgat gaggcaaat gttcattat 
      caattcaca atcgatcag tacacttta ggcttgaga 
      tacctcgaa gatagacta gggaagtgt tccaggccc 
      aaagtttgc tggcaaatt gatcctttc ggacacagc 
      agagaacaa gcctcgatt tcggcccag ctgggcttt 
      gatagcgtt tttttcgca cggttggat tacagagac 
      cggattaat cgactaggg accaagacc ggcgatttg 
      atttggaga gggagcagc aatttgggc aacagttcg 
      gatattttc acctcagtg atgtataac cactacagc 
      gcacctgcc ggtttttgt ttcgatatt gtttgtaat 
      gacgaaccg attatagac gatgaggaa agtcctgac 
      tacaattgg aaaagtcga gtcgaagct tttgccaat 
      tttgttcgc gaacaagcg tccaaatac ccaaccaac 
      aatattcta gtacccatg ggagacgat ttcaggtac 
      caagccgcc ttgaacgcc tatattaat acggataga 
      ctgatcaaa ggttttgag ctgtttccc caaactttt 
      gagggcaaa cccatcaag ctactgtac tcaacacca 
      acgtgttac actaaagcc gtcaatgat ttcgtcaat 
      tcaaataat tacaaactg caaatcaag agtgatgat 
      tttttcccc tatgctgat ggggttgca agtttagta 
      gttgggtac tttacatca agaccggcc tcgaaacgt 
      ttcgtccgg gaagcaaac accatgctt caggtggcc 
      aaacaacta gcagctgtt gcaaactta ccctacgaa 
      aacgaaaac attaactcg ctcaaagaa gcaatgggt 
      gttttgcaa caccatgat gctataaca ggtactgaa 
      ctaattgac gtgacccat gactaccac cgaatcctt 
      cacaaaggg attagtgac gctgttaat gccatcaac 
      cccatttta tcaaattta ataagcggt tctgattcc 
      ctaaaatta gaatcgtgt cttttggct aacgttagc 
      gtctgtctc caagcaaaa gctgaccag tttatttta 
      acagtctac aatccattg agtcgagtt gtttcaagt 
      ccaatcagc attccagta gagacagaa aattggaaa 
      attttagac tccaatggt gacgaagtt gtgtaccag 
      gtggtacct tcaatcacc gattttagc accctggtg 
      gatgaaaca acctcgccc aactctctc gattttatc 
      gcaaaagat attccagct ctaggctac aaagtctat 
      actttcaca aaagtggcc acaaagccc acaaaaaga 
      attactgta ggtgccgat ggtaccacc ttcgaaata 
      gacgaagtc tctggtctt ttaacatca gtgaccatg 
      aatggagtc actcttaac gtaacgcaa gattttctc 
      gtttataaa agtggtgga cggtcacaa gcttacgtt 
      tttgaccct gatggtgac ccacaaaga gttgcctca 
      actgtcaca gtcaagaaa atcgaagga gatgtctat 
      tacggagta gtacaagaa tttagcgat tgggcgaaa 
      caaataatc aaagtctat aaaaatgat agtagttac 
      attgagttt gattgggtc attggaccc ctaaacatc 
      gacgatggt ggcaaagaa gtcatctcg caattttcg 
      acacctttg caaaccgaa ggtgttttc tacaccgac 
      tccaatggt cgcgaaata ttaaaaagg gtacgcaac 
      caacgtcca gattacgac tacactgac gaacaaccc 
      attgctgga aattactac ccagtgact tcgaaaatt 
      ttgattcgg gacgaaaat ctgggtgtg gagatggca 
      attttgaat gatcgggcc caaggtggg tcaagtgta 
      aaagatggg caagtggaa ctgatggta catcgaaga 
      attaggtca ggcgatggt ttcggctta gatgagata 
      gagtatggg catgggatt gtcgtacga gggaaacat 
      ttcctagtt ttgggacca gtttcgggc aatggtgaa 
      aagtctttg gctgcgatt gaacgtgat gtcgcccaa 
      aggaaattg ttaccacct tgggtgttt atcaccaat 
      caagatgtg agcggttcc aaaaattta gaatatagt 
      agtttgaag agaccattg cctgataat gtccaattg 
      ttaacgctt gaaccctgg tctgatggt actcttctc 
      gtgcgtttg gagcacgtt ttggagaaa aatgaggat 
      gaaaattta tcaaaagaa gtcacagtt gatctttcg 
      gatttgttt gtcgcgttc actgttcaa gaactcaag 
      gaaacaact ctagctggt aacatcccc attgaagag 
      aacgtccgt ctgcactgg cctggtagt accaacgag 
      gcccctcca caagcccct cgggccccc accgacttg 
      accatcacc ctagccccc atgcaaatc cgcactttc 
      ctagccaca gtcacttac aacaatctg tag
    mRNA sequence
    • >TC002467 RA
      caagtgttg ataaacaaa acttttgtt ttactaagt 
      ggcaaaaaa acattctgt tcgcacact tggaacaga 
      catgttact aaaacttct atttttggt actcttggg 
      ccaaattag ggcaaagcc ggtcgaaga cgagccagt 
      ttgtggata tgaggcttg ccctgcgac caacttctc 
      cacactcaa cgtccacct catccctca ctcccatga 
      cgacgtcgg ttggctcaa aaccatgga ccagtacta 
      cttccaaga cgtccagaa cgtgatcag taccgtaat 
      cccagcttt gaagcaaaa ccctgaccg tcgtttcgt 
      ccaagtcga aacagcgtt ttttaaaaa atggtggga 
      acagcaaaa ggactcagt caagcaaga tgttatcaa 
      actggtcaa taacgggca gttcgagat aattaacgg 
      cgcctggag catgaatga tgaggcaaa tgttcatta 
      tcaattcac aatcgatca gtacacttt aggcttgag 
      atacctcga agatagact agggaagtg ttccaggcc 
      caaagtttg ctggcaaat tgatccttt cggacacag 
      cagagaaca agcctcgat ttcggccca gctgggctt 
      tgatagcgt ttttttcgc acggttgga ttacagaga 
      ccggattaa tcgactagg gaccaagac cggcgattt 
      gatttggag agggagcag caatttggg caacagttc 
      ggatatttt cacctcagt gatgtataa ccactacag 
      cgcacctgc cggtttttg tttcgatat tgtttgtaa 
      tgacgaacc gattataga cgatgagga aagtcctga 
      ctacaattg gaaaagtcg agtcgaagc ttttgccaa 
      ttttgttcg cgaacaagc gtccaaata cccaaccaa 
      caatattct agtacccat gggagacga tttcaggta 
      ccaagccgc cttgaacgc ctatattaa tacggatag 
      actgatcaa aggttttga gctgtttcc ccaaacttt 
      tgagggcaa acccatcaa gctactgta ctcaacacc 
      aacgtgtta cactaaagc cgtcaatga tttcgtcaa 
      ttcaaataa ttacaaact gcaaatcaa gagtgatga 
      ttttttccc ctatgctga tggggttgc aagtttagt 
      agttgggta ctttacatc aagaccggc ctcgaaacg 
      tttcgtccg ggaagcaaa caccatgct tcaggtggc 
      caaacaact agcagctgt tgcaaactt accctacga 
      aaacgaaaa cattaactc gctcaaaga agcaatggg 
      tgttttgca acaccatga tgctataac aggtactga 
      actaattga cgtgaccca tgactacca ccgaatcct 
      tcacaaagg gattagtga cgctgttaa tgccatcaa 
      ccccatttt atcaaattt aataagcgg ttctgattc 
      cctaaaatt agaatcgtg tcttttggc taacgttag 
      cgtctgtct ccaagcaaa agctgacca gtttatttt 
      aacagtcta caatccatt gagtcgagt tgtttcaag 
      tccaatcag cattccagt agagacaga aaattggaa 
      aattttaga ctccaatgg tgacgaagt tgtgtacca 
      ggtggtacc ttcaatcac cgattttag caccctggt 
      ggatgaaac aacctcgcc caactctct cgattttat 
      cgcaaaaga tattccagc tctaggcta caaagtcta 
      tactttcac aaaagtggc cacaaagcc cacaaaaag 
      aattactgt aggtgccga tggtaccac cttcgaaat 
      agacgaagt ctctggtct tttaacatc agtgaccat 
      gaatggagt cactcttaa cgtaacgca agattttct 
      cgtttataa aagtggtgg acggtcaca agcttacgt 
      ttttgaccc tgatggtga cccacaaag agttgcctc 
      aactgtcac agtcaagaa aatcgaagg agatgtcta 
      ttacggagt agtacaaga atttagcga ttgggcgaa 
      acaaataat caaagtcta taaaaatga tagtagtta 
      cattgagtt tgattgggt cattggacc cctaaacat 
      cgacgatgg tggcaaaga agtcatctc gcaattttc 
      gacaccttt gcaaaccga aggtgtttt ctacaccga 
      ctccaatgg tcgcgaaat attaaaaag ggtacgcaa 
      ccaacgtcc agattacga ctacactga cgaacaacc 
      cattgctgg aaattacta cccagtgac ttcgaaaat 
      tttgattcg ggacgaaaa tctgggtgt ggagatggc 
      aattttgaa tgatcgggc ccaaggtgg gtcaagtgt 
      aaaagatgg gcaagtgga actgatggt acatcgaag 
      aattaggtc aggcgatgg tttcggctt agatgagat 
      agagtatgg gcatgggat tgtcgtacg agggaaaca 
      tttcctagt tttgggacc agtttcggg caatggtga 
      aaagtcttt ggctgcgat tgaacgtga tgtcgccca 
      aaggaaatt gttaccacc ttgggtgtt tatcaccaa 
      tcaagatgt gagcggttc caaaaattt agaatatag 
      tagtttgaa gagaccatt gcctgataa tgtccaatt 
      gttaacgct tgaaccctg gtctgatgg tactcttct 
      cgtgcgttt ggagcacgt tttggagaa aaatgagga 
      tgaaaattt atcaaaaga agtcacagt tgatctttc 
      ggatttgtt tgtcgcgtt cactgttca agaactcaa 
      ggaaacaac tctagctgg taacatccc cattgaaga 
      gaacgtccg tctgcactg gcctggtag taccaacga 
      ggcccctcc acaagcccc tcgggcccc caccgactt 
      gaccatcac cctagcccc catgcaaat ccgcacttt 
      cctagccac agtcactta caacaatct gtagtacca 
      caagttgaa taaattgtt acgatttaa