Status
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Gene information
General information
TC002464 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28049247..28060864
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002464
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00380

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks - cuticles: eggs lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002464 RA
      MFRRTLVPA WIFAGVCFH LCATAAIKS ATQCGYQAC 
      NPIKDGFIN VHLVPHTHD DVGWLKTVD QYFYGANNS 
      IQNAGVQYI IDSVIDELK KDPKRKFIY VETAYFWKW 
      WIHQHDFVK HQVKTYVNN GQLEFIGGA WTMNDEATT 
      HYQSIIDQF TWGLRKLND TFGDCGRPK IGWQIDPFG 
      HSREMASIF AQLGFDGLL LGRIDYEDK DKRFESKTP 
      EMIWKGSDN LDADIFTGV MYNTYAPPK GFCFDIMCD 
      DEPLVDDKK SPLYNIERK VDDFFAYLD NVTKAYTTS 
      NVIITMGED FNYQNAHTW FKNLDKLIY YANQRQING 
      SKYNLLYST PSCYTKAVH DSNQKFVSK TDDFFPYSS 
      DGNSFWTGY FTSRPTLKR FERQGNNFL QVCKQLYAL 
      VDLGPEDWV DLNALREAM GVMQHHDAI TGTEKQHVA 
      EDYARILQG GIDECQFIV NTALSKIIT GENSTGPDP 
      GPKFPINTC WLTNTSSCP FSEDQDNFL VTVYNPLSR 
      PVTKYVRLP VIGEAYNVK CPQGKELLT QLIPIPDAV 
      KNMPGRVSK ATVELIFEA PFVPPLGFK TFTVSKKTG 
      NEVLKAEKI TKIADGEVG FTLDPNSGL LKTVILNGK 
      TVDVNQEFL YYEGFVGDN EEPKNRSSG AYIFRPIPG 
      KDPVVVADK VDYKIFRGN LVSEVQQVF NEWITQTIR 
      VYRTESYIE FDWIVGPIP DTDANGKEI ITRFTTPLN 
      TKSTFYTDS NGREMLKRV RNARPTWTL TLEEPVSGN 
      YYPVTSKIV LVDESQDLE LAVLTDRAQ GGTSLQDGQ 
      LELMVHRNC LHDDAFGVA EALNETAFG KGLVARGSH 
      FLTLGPHFK KTGNVSTAA LERDIAQRK VLDSWVFIS 
      APYNDSKYI KEFSGLKRA LPANVQILT LEPWKGFSF 
      LLRLEHVLE KGEDADLSQ PAIVNLQNL FTPFEIKSI 
      RETTLGANQ WLEKNERLE FEAKDLFRK NEKRATLIR 
      ALDDYQITL NPMQIRTFV IEIEKN
    CDS sequence
    • >TC002464 RA
      atgttcaga agaacgcta gtcccggcg tggattttt 
      gccggagtt tgtttccac ttgtgtgca actgctgcc 
      atcaaaagt gcaacccaa tgcggatat caggcgtgt 
      aatccaatc aaggatggg tttatcaac gtccacctt 
      gtccctcac acccatgac gatgttggg tggctcaaa 
      accgtcgat cagtatttc tacggagcc aataattcg 
      atccaaaac gctggtgtt caatacatc attgattca 
      gtcattgac gagttgaag aaagacccc aagagaaaa 
      tttatctac gttgaaaca gcctatttt tggaagtgg 
      tggatccac caacacgat ttcgtcaaa caccaagtc 
      aaaacttac gtcaacaac ggccaattg gagttcatt 
      ggaggtgcc tggactatg aatgatgaa gcaacaacg 
      cactaccaa tcaatcatt gaccagttc acttggggt 
      ctgaggaaa ttgaatgat acgtttggc gactgtggt 
      cgtcctaaa atcggctgg caaatcgac ccgtttggg 
      cacagccgt gagatggcc tcgattttt gcccaactg 
      ggcttcgat gggcttctc ctgggccgt attgactat 
      gaggataaa gacaagaga tttgagtct aaaacaccg 
      gaaatgata tggaaaggt agtgataac ctggacgct 
      gatattttc actggagtc atgtataac acgtacgcg 
      cctccgaaa ggcttctgt tttgatatt atgtgcgac 
      gatgagcca cttgttgat gataagaaa agtccactt 
      tacaatatc gagcggaaa gttgatgat ttcttcgcg 
      tatcttgat aatgtaacg aaagcttac accacgtct 
      aatgttatc atcacaatg ggggaggat ttcaactac 
      cagaatgca cacacgtgg ttcaaaaat ttggataag 
      cttatctat tatgccaac caaagacaa atcaacggc 
      tccaaatac aacctcctc tactccacc ccctcctgc 
      tacaccaaa gccgtccac gactccaac caaaaattc 
      gtctcgaaa accgacgac ttcttcccc tactcctcg 
      gacggcaac tccttctgg accggttac ttcacctcc 
      cggcccact ctaaagcgc ttcgagcgc caaggcaac 
      aacttcctc caagtctgc aagcaactc tacgcctta 
      gtagacctc ggtcctgaa gactgggtc gatttgaac 
      gctctgaga gaagccatg ggtgtgatg cagcaccac 
      gatgccatc acaggcacg gaaaaacaa cacgtggca 
      gaagactac gcccggatc ctccaaggg ggcattgac 
      gagtgccaa tttatcgta aacacagcc ttgagtaaa 
      atcatcaca ggggagaac agcacaggg cctgacccg 
      gggcccaaa ttcccgatt aatacgtgt tggttgacc 
      aacaccagc tcctgtcct tttagtgaa gatcaggat 
      aatttcctt gttacggtt tataatcct ttgagtcgt 
      cctgtgacc aaatatgtg agactgcca gtcattggg 
      gaggcttac aatgtcaag tgcccacaa ggcaaggaa 
      cttttgacc caactgatc cctattcca gacgctgtg 
      aaaaatatg ccaggtcgt gtgagcaaa gcaacagtt 
      gagcttatt tttgaagca ccgtttgtg ccaccactt 
      ggcttcaaa accttcaca gtgagcaaa aaaaccggg 
      aatgaagta ctcaaagcg gaaaaaatc accaaaatt 
      gctgatggg gaagttggt tttacgctg gacccgaat 
      tcaggtctc ttgaagaca gttattttg aacgggaag 
      acggttgat gttaaccaa gagtttctc tactatgaa 
      gggtttgtc ggtgataat gaagagccg aaaaatcgg 
      tcgtcggga gcgtacatt ttccggcca attccgggc 
      aaggatccg gtcgttgta gcggacaag gtggattat 
      aaaattttc agagggaat ttggtttct gaggtgcag 
      caagttttc aacgagtgg atcactcaa acaattcgg 
      gtttatcga acggagagc tacatcgag tttgattgg 
      attgtgggc ccaattcca gacacagac gccaacggc 
      aaagaaata atcactcgc ttcaccaca cccctcaac 
      accaaatcc accttctac acggactca aacggccga 
      gaaatgctc aaacgtgtc cgaaacgcc cgccccacc 
      tggaccctc accctcgaa gaacccgtt tctggcaac 
      tactacccc gtcacttca aaaatagtc ctcgtggac 
      gagtcccaa gacctggag ctggccgtt ttgactgat 
      agagcccaa ggtggtacc agtctccag gacggacaa 
      ctcgaactc atggtccat cggaactgc ctccatgac 
      gatgctttc ggagtcgct gaagccctc aacgagacg 
      gcgttcggc aaaggcctg gtggccaga gggtcgcat 
      tttttgact cttggaccc cattttaaa aaaacggga 
      aatgtgagc acagccgcc ttggaaagg gatatcgcc 
      cagaggaag gttctggat tcgtgggtg tttatctcg 
      gcaccttac aatgactcc aaatatatt aaagagttt 
      tcaggcctg aaacgcgct ttgcctgcc aacgtccaa 
      atcctgacc ctggagcct tggaaaggc ttctctttc 
      cttctccgc ctagaacac gttttggaa aagggcgaa 
      gatgcggat ttatctcaa ccagcaatc gtcaacttg 
      cagaatttg ttcacacct ttcgaaatt aaatcaatt 
      cgtgaaacc acgctcgga gccaatcaa tggttggag 
      aagaacgaa cgtttggaa tttgaagcg aaagacttg 
      ttccggaag aacgagaag cgagcaact ctgataaga 
      gcccttgac gactatcag ataacgctg aatccgatg 
      caaatccga acttttgta atagaaata gagaagaat 
      tga
    mRNA sequence
    • >TC002464 RA
      ttcatcctc aacatgttc agaagaacg ctagtcccg 
      gcgtggatt tttgccgga gtttgtttc cacttgtgt 
      gcaactgct gccatcaaa agtgcaacc caatgcgga 
      tatcaggcg tgtaatcca atcaaggat gggtttatc 
      aacgtccac cttgtccct cacacccat gacgatgtt 
      gggtggctc aaaaccgtc gatcagtat ttctacgga 
      gccaataat tcgatccaa aacgctggt gttcaatac 
      atcattgat tcagtcatt gacgagttg aagaaagac 
      cccaagaga aaatttatc tacgttgaa acagcctat 
      ttttggaag tggtggatc caccaacac gatttcgtc 
      aaacaccaa gtcaaaact tacgtcaac aacggccaa 
      ttggagttc attggaggt gcctggact atgaatgat 
      gaagcaaca acgcactac caatcaatc attgaccag 
      ttcacttgg ggtctgagg aaattgaat gatacgttt 
      ggcgactgt ggtcgtcct aaaatcggc tggcaaatc 
      gacccgttt gggcacagc cgtgagatg gcctcgatt 
      tttgcccaa ctgggcttc gatgggctt ctcctgggc 
      cgtattgac tatgaggat aaagacaag agatttgag 
      tctaaaaca ccggaaatg atatggaaa ggtagtgat 
      aacctggac gctgatatt ttcactgga gtcatgtat 
      aacacgtac gcgcctccg aaaggcttc tgttttgat 
      attatgtgc gacgatgag ccacttgtt gatgataag 
      aaaagtcca ctttacaat atcgagcgg aaagttgat 
      gatttcttc gcgtatctt gataatgta acgaaagct 
      tacaccacg tctaatgtt atcatcaca atgggggag 
      gatttcaac taccagaat gcacacacg tggttcaaa 
      aatttggat aagcttatc tattatgcc aaccaaaga 
      caaatcaac ggctccaaa tacaacctc ctctactcc 
      accccctcc tgctacacc aaagccgtc cacgactcc 
      aaccaaaaa ttcgtctcg aaaaccgac gacttcttc 
      ccctactcc tcggacggc aactccttc tggaccggt 
      tacttcacc tcccggccc actctaaag cgcttcgag 
      cgccaaggc aacaacttc ctccaagtc tgcaagcaa 
      ctctacgcc ttagtagac ctcggtcct gaagactgg 
      gtcgatttg aacgctctg agagaagcc atgggtgtg 
      atgcagcac cacgatgcc atcacaggc acggaaaaa 
      caacacgtg gcagaagac tacgcccgg atcctccaa 
      gggggcatt gacgagtgc caatttatc gtaaacaca 
      gccttgagt aaaatcatc acaggggag aacagcaca 
      gggcctgac ccggggccc aaattcccg attaatacg 
      tgttggttg accaacacc agctcctgt ccttttagt 
      gaagatcag gataatttc cttgttacg gtttataat 
      cctttgagt cgtcctgtg accaaatat gtgagactg 
      ccagtcatt ggggaggct tacaatgtc aagtgccca 
      caaggcaag gaacttttg acccaactg atccctatt 
      ccagacgct gtgaaaaat atgccaggt cgtgtgagc 
      aaagcaaca gttgagctt atttttgaa gcaccgttt 
      gtgccacca cttggcttc aaaaccttc acagtgagc 
      aaaaaaacc gggaatgaa gtactcaaa gcggaaaaa 
      atcaccaaa attgctgat ggggaagtt ggttttacg 
      ctggacccg aattcaggt ctcttgaag acagttatt 
      ttgaacggg aagacggtt gatgttaac caagagttt 
      ctctactat gaagggttt gtcggtgat aatgaagag 
      ccgaaaaat cggtcgtcg ggagcgtac attttccgg 
      ccaattccg ggcaaggat ccggtcgtt gtagcggac 
      aaggtggat tataaaatt ttcagaggg aatttggtt 
      tctgaggtg cagcaagtt ttcaacgag tggatcact 
      caaacaatt cgggtttat cgaacggag agctacatc 
      gagtttgat tggattgtg ggcccaatt ccagacaca 
      gacgccaac ggcaaagaa ataatcact cgcttcacc 
      acacccctc aacaccaaa tccaccttc tacacggac 
      tcaaacggc cgagaaatg ctcaaacgt gtccgaaac 
      gcccgcccc acctggacc ctcaccctc gaagaaccc 
      gtttctggc aactactac cccgtcact tcaaaaata 
      gtcctcgtg gacgagtcc caagacctg gagctggcc 
      gttttgact gatagagcc caaggtggt accagtctc 
      caggacgga caactcgaa ctcatggtc catcggaac 
      tgcctccat gacgatgct ttcggagtc gctgaagcc 
      ctcaacgag acggcgttc ggcaaaggc ctggtggcc 
      agagggtcg cattttttg actcttgga ccccatttt 
      aaaaaaacg ggaaatgtg agcacagcc gccttggaa 
      agggatatc gcccagagg aaggttctg gattcgtgg 
      gtgtttatc tcggcacct tacaatgac tccaaatat 
      attaaagag ttttcaggc ctgaaacgc gctttgcct 
      gccaacgtc caaatcctg accctggag ccttggaaa 
      ggcttctct ttccttctc cgcctagaa cacgttttg 
      gaaaagggc gaagatgcg gatttatct caaccagca 
      atcgtcaac ttgcagaat ttgttcaca cctttcgaa 
      attaaatca attcgtgaa accacgctc ggagccaat 
      caatggttg gagaagaac gaacgtttg gaatttgaa 
      gcgaaagac ttgttccgg aagaacgag aagcgagca 
      actctgata agagccctt gacgactat cagataacg 
      ctgaatccg atgcaaatc cgaactttt gtaatagaa 
      atagagaag aattgattg ttttattga caaatatag 
      acacttgtg ataagttac agatacaat caaaacact 
      gttactaac tgtatggtt cctataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY