Status
Please wait ...
Gene information
General information
TC002463 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29979989..29988597
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002463
    iBeetle screen iB_00379

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002463 RA
      MAYRLISSS KKLEKLLFR NLKQCNFNI SKENSTFQA 
      VLNQWRLFC EKPPKGFEK YFEPGSKQK GKSSKTEPP 
      KEAKEAKKD GKQEQPKPP PKTPSGQQG QKPYDQWSF 
      GLFGGTGSR GSGGKPFGD GDRDKWYIL GAGAAIAFL 
      ATVTMWEMG YKEISWKEF VQSYLTRGI VEKLEVVNK 
      KWVRVRLLP GNTIDGANF VWFNIGSVD SFERNLENA 
      QIEMNVEPP NFVPVIYKT EVEAASLSG VLPTILVIG 
      FLIYMMRRS AEMMGGGKG KRKGLFGSV MESTAKLIN 
      SNEIGVRFK DVAGCEEAK IEIMEFVNF LKNPQQYID 
      LGAKIPKGA MLTGPPGTG KTLLAKATA GEANVPFIT 
      VSGSEFLEM FVGVGPSRV RDLFSMARK HAPCILFID 
      EIDAVGRKR GGRSFGGHS EQENTLNQL LVEMDGFNT 
      TTNVVVLAA TNRIDILDK ALLRPGRFD RQIFVPAPD 
      IKGRASIFK VHLGPLKTN LDKSELARK MAALTPGFT 
      GADIANVCN EAALIAARD LNESINMKN FEQAIERVV 
      AGMEKKTNV LSPEEKKTV AYHEAGHAV AGWFLRYAD 
      PLLKVSIIP RGKGLGYAQ YLPKDQYLY TKEQLFDRM 
      CMTLGGRVS EELFFQRIT TGAQDDLKK VTQSAYAQV 
      VHYGMNEKV GNVSFDMPR EGEMMLEKP YSESTAQMI 
      DVEVRNLID QAYKRTTAL LTEHKENVA RVAERLLKQ 
      EIISREDMI ELLGKRPFP EKSTYEEFV EGTGSLEED 
      TSLPEGLKD WNKEKAAEQ QPQKSQA
    CDS sequence
    • >TC002463 RA
      atggcatat cgtctcatt agtagcagc aaaaaactg 
      gaaaaattg ctatttaga aacttgaaa caatgcaac 
      tttaacata agcaaagag aattcgacg tttcaagcc 
      gttctaaac cagtggaga ctcttttgc gaaaagcct 
      cctaaaggt ttcgaaaaa tatttcgaa cccggctcg 
      aaacagaag ggcaaaagt tcgaaaact gagcctccg 
      aaggaggcc aaagaagcg aagaaagat ggcaagcag 
      gagcagcca aagccgccc ccgaagacc ccaagcggt 
      cagcagggg cagaagccg tacgaccag tggtctttc 
      gggcttttc gggggcaca gggagccgg gggagtggc 
      ggcaagccc ttcggggac ggcgaccgg gacaagtgg 
      tacattctg ggggcgggg gcggccatt gcgttccta 
      gccactgtg accatgtgg gagatgggg tacaaggag 
      atcagctgg aaggagttt gtacaaagt tacttaaca 
      cgagggatt gtagaaaag ttagaggtt gttaataag 
      aagtgggtc agagtgagg cttctccct gggaatacc 
      attgacggt gctaatttt gtatggttt aatattggt 
      agtgtagat tcgtttgaa cgcaactta gaaaatgcc 
      cagattgag atgaacgta gagccacct aattttgta 
      ccagttatt tacaagact gaagtggaa gctgcaagt 
      ttgtccggg gttttaccc acaattcta gttattgga 
      tttttgatc tacatgatg agacgatct gccgaaatg 
      atgggcggc ggcaagggg aagaggaaa ggccttttt 
      ggcagtgta atggaaagc acagccaaa ttaatcaat 
      tccaatgaa attggagtc agattcaaa gacgtcgct 
      ggttgtgag gaggctaaa atcgaaatc atggaattc 
      gtcaacttc ctcaaaaac cctcaacaa tacatcgac 
      ctcggggcc aaaatcccc aaaggcgcc atgttgaca 
      ggcccacct ggcaccggt aaaaccctc ctagccaag 
      gccaccgca ggtgaagcc aacgtcccg ttcatcaca 
      gtttccggg tccgaattc ctggaaatg ttcgtaggt 
      gtgggtcca tcccgtgta cgcgacctc ttctccatg 
      gcccgcaaa cacgctcct tgtatcctg tttattgac 
      gaaatcgac gccgtgggc cgaaaacgc gggggtcgc 
      agttttggg ggccactca gagcaggaa aacaccctg 
      aaccagctt ctggtggaa atggacggt tttaacaca 
      acgacaaat gttgtggtt ttggcggca acaaatcgt 
      atagatatc ttggacaaa gccttgcta aggcctggc 
      cgtttcgac cggcaaatc ttcgtcccg gcccccgat 
      atcaaaggg cgcgctagc attttcaaa gtccatcta 
      gggcccttg aaaacaaac ttggacaaa agcgagttg 
      gcgcgcaaa atggccgcc ctcacgccg ggattcacc 
      ggcgccgac attgccaat gtttgcaac gaggcggcg 
      ctgatcgcg gcgcgagat ttgaacgag tcgatcaat 
      atgaagaat ttcgagcaa gcgattgag cgagtggtc 
      gccgggatg gagaaaaag accaatgtt ttgtcgccc 
      gaggagaag aagacagtg gcgtatcac gaagctggg 
      catgctgtt gctgggtgg tttctacgc tacgccgat 
      ccgctcctc aaagtttcg ataatcccg cgtggaaaa 
      ggcctgggt tatgcgcag tatttgccc aaagaccag 
      tatttgtac acgaaggag cagctcttc gaccgcatg 
      tgcatgacg ctcgggggg cgcgtctcc gaggagctt 
      ttcttccag aggattacc actggggct caggacgat 
      ttgaagaag gtgacgcag agtgcgtat gcacaagtc 
      gtgcattat ggcatgaat gagaaggtg gggaatgtt 
      agtttcgat atgccgagg gagggggaa atgatgttg 
      gaaaaaccc tactcggag agtacagca cagatgatt 
      gatgtcgaa gttaggaat ttgattgac caggcgtat 
      aaacgaacc actgcgctt ctgacggag cacaaggag 
      aacgtggcc agagtggcg gagcgactc ctgaagcag 
      gagattatc agtcgggag gatatgatc gagttgttg 
      gggaagagg ccgttcccg gaaaagtcg acctatgag 
      gagtttgtg gaagggacg gggtcgttg gaggaggat 
      acgagtttg ccggaaggc ttgaaggat tggaataag 
      gagaaggcg gccgagcaa cagccgcag aaatcgcag 
      gcttga
    mRNA sequence
    • >TC002463 RA
      gatgctggg tggtttttt acaaaacta atcaacctt 
      gacgacggg tattataat tcttaaaaa ataaatagt 
      tatgcaaat aaaaaacaa atagtttaa ttcagaaat 
      tcgctctga aaggttttt ttttgagag ttattgcaa 
      gacagggtc tccaacaca caaaaactt ctcccccac 
      tccatctgt caaaaaata aattcaagt gtgaggtta 
      gttatgtga taaaaatac tttcttttc caccaattt 
      gctatggca tatcgtctc attagtagc agcaaaaaa 
      ctggaaaaa ttgctattt agaaacttg aaacaatgc 
      aactttaac ataagcaaa gagaattcg acgtttcaa 
      gccgttcta aaccagtgg agactcttt tgcgaaaag 
      cctcctaaa ggtttcgaa aaatatttc gaacccggc 
      tcgaaacag aagggcaaa agttcgaaa actgagcct 
      ccgaaggag gccaaagaa gcgaagaaa gatggcaag 
      caggagcag ccaaagccg cccccgaag accccaagc 
      ggtcagcag gggcagaag ccgtacgac cagtggtct 
      ttcgggctt ttcgggggc acagggagc cgggggagt 
      ggcggcaag cccttcggg gacggcgac cgggacaag 
      tggtacatt ctgggggcg ggggcggcc attgcgttc 
      ctagccact gtgaccatg tgggagatg gggtacaag 
      gagatcagc tggaaggag tttgtacaa agttactta 
      acacgaggg attgtagaa aagttagag gttgttaat 
      aagaagtgg gtcagagtg aggcttctc cctgggaat 
      accattgac ggtgctaat tttgtatgg tttaatatt 
      ggtagtgta gattcgttt gaacgcaac ttagaaaat 
      gcccagatt gagatgaac gtagagcca cctaatttt 
      gtaccagtt atttacaag actgaagtg gaagctgca 
      agtttgtcc ggggtttta cccacaatt ctagttatt 
      ggatttttg atctacatg atgagacga tctgccgaa 
      atgatgggc ggcggcaag gggaagagg aaaggcctt 
      tttggcagt gtaatggaa agcacagcc aaattaatc 
      aattccaat gaaattgga gtcagattc aaagacgtc 
      gctggttgt gaggaggct aaaatcgaa atcatggaa 
      ttcgtcaac ttcctcaaa aaccctcaa caatacatc 
      gacctcggg gccaaaatc cccaaaggc gccatgttg 
      acaggccca cctggcacc ggtaaaacc ctcctagcc 
      aaggccacc gcaggtgaa gccaacgtc ccgttcatc 
      acagtttcc gggtccgaa ttcctggaa atgttcgta 
      ggtgtgggt ccatcccgt gtacgcgac ctcttctcc 
      atggcccgc aaacacgct ccttgtatc ctgtttatt 
      gacgaaatc gacgccgtg ggccgaaaa cgcgggggt 
      cgcagtttt gggggccac tcagagcag gaaaacacc 
      ctgaaccag cttctggtg gaaatggac ggttttaac 
      acaacgaca aatgttgtg gttttggcg gcaacaaat 
      cgtatagat atcttggac aaagccttg ctaaggcct 
      ggccgtttc gaccggcaa atcttcgtc ccggccccc 
      gatatcaaa gggcgcgct agcattttc aaagtccat 
      ctagggccc ttgaaaaca aacttggac aaaagcgag 
      ttggcgcgc aaaatggcc gccctcacg ccgggattc 
      accggcgcc gacattgcc aatgtttgc aacgaggcg 
      gcgctgatc gcggcgcga gatttgaac gagtcgatc 
      aatatgaag aatttcgag caagcgatt gagcgagtg 
      gtcgccggg atggagaaa aagaccaat gttttgtcg 
      cccgaggag aagaagaca gtggcgtat cacgaagct 
      gggcatgct gttgctggg tggtttcta cgctacgcc 
      gatccgctc ctcaaagtt tcgataatc ccgcgtgga 
      aaaggcctg ggttatgcg cagtatttg cccaaagac 
      cagtatttg tacacgaag gagcagctc ttcgaccgc 
      atgtgcatg acgctcggg gggcgcgtc tccgaggag 
      cttttcttc cagaggatt accactggg gctcaggac 
      gatttgaag aaggtgacg cagagtgcg tatgcacaa 
      gtcgtgcat tatggcatg aatgagaag gtggggaat 
      gttagtttc gatatgccg agggagggg gaaatgatg 
      ttggaaaaa ccctactcg gagagtaca gcacagatg 
      attgatgtc gaagttagg aatttgatt gaccaggcg 
      tataaacga accactgcg cttctgacg gagcacaag 
      gagaacgtg gccagagtg gcggagcga ctcctgaag 
      caggagatt atcagtcgg gaggatatg atcgagttg 
      ttggggaag aggccgttc ccggaaaag tcgacctat 
      gaggagttt gtggaaggg acggggtcg ttggaggag 
      gatacgagt ttgccggaa ggcttgaag gattggaat 
      aaggagaag gcggccgag caacagccg cagaaatcg 
      caggcttga gtgtagtgt gtggggact ttgagtttg 
      aaatccatt aataaatat tatttaata tagaagttg 
      ttcttttat ttgttatgt ggatcaaaa taaacgtac 
      aaatgattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY