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Gene information
General information
TC002462 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid29: 1373484..1396001
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002462
    iBeetle screen iB_00378
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    larval musculature degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    Pupal morphology
    hindwing distal pigmentation/tanning not present - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002462 RA
      MAEAHSAVA FSFSITHEG WDVNFDREV LHLVWQSGI 
      RSWKKRIAR FQNSIHNGV YPGHLWSLW LIFSIVTAV 
      HYMDRAVPF DLAAKFLYL FNDTSTRWQ LLACFLAAL 
      VYWLAIIFT LRYTLKLLL MYKGWMYEA RGKGTKTSL 
      PTKMWFICV RIFSGWNTP KLYSFQGSL PRLPLPSLH 
      DTMSRYLRS VRPLLDDKN YQRMERLAS EFENGIGKK 
      LQRYLWLKS WWSSNYVSD WWEEYVYLR GRSPLMINS 
      NFYGIDAIF MTPTSIQAA RAASVINSC LVMRRLVER 
      QELEPMMIQ GVVPLCSWQ YERIFNTTR IPGMETDKI 
      QHWQDSNHI VVYHKGRYF KVVIYKGRI LKPCEIQVQ 
      IEQILNDKS EPLPGEEKL AALTAGERT HWANVRRMF 
      FTKGVNKTS LDIIEKAAF VVALDDIPY EFDQKDPSK 
      LDNYGKILL HGKGYDRWF DKSFTLCVG TNGRIGFNA 
      EHSWADAAV ISHVWEWVV TTQIHETRY DKTGNTLGK 
      PELTPPTPT RLNWEIRFE CQEAIDKSM KVVKSLIED 
      IDLRIYVHD HYGKGFIKQ CRLSPDAYL QMALQLAYY 
      RDAGRFDLT YEASMTRLF REGRTETVR PCTIEASNW 
      VKAMENPKS TTEERVQLL RTACQRHQV AYQDAMCGK 
      GIDRHLFCL YVVSKYLEV DSPFLQEVL SEPWRLSTS 
      QTPHGQTGR MDLNKYPEC ISAGGGFGP VADDGYGVS 
      YIIAGEDMF FFHVSNKRS SSKTDCHRF AKRIEKALA 
      DMKALYIDY KSQNGNK
    CDS sequence
    • >TC002462 RA
      atggcggaa gcccattca gctgttgct ttctcgttt 
      tccatcacc cacgaaggc tgggacgtc aacttcgac 
      agagaagtc cttcacctg gtgtggcaa tccggtatt 
      cgctcttgg aagaagcga atcgccagg tttcagaat 
      agcattcac aatggtgta tatccaggc catttgtgg 
      agtttgtgg ttgatattt tccattgtc acggctgtg 
      cattacatg gacagggcg gttcccttt gatcttgct 
      gcgaaattt ttatattta tttaatgat acttccacg 
      cgatggcaa ctcctcgcg tgcttttta gcagcatta 
      gtttattgg ttagctata atattcacc cttaggtac 
      acattaaaa ttattactt atgtacaag ggttggatg 
      tatgaagcg agaggtaaa ggcactaaa acatctttg 
      cctacgaaa atgtggttc atatgtgtt agaattttt 
      agcggctgg aatacacca aaactttac agtttccaa 
      ggctcgctg ccacgttta cctctgccc tccttgcac 
      gacaccatg agcagatat ttaaggagc gttcgtccg 
      ctcctcgac gataaaaac taccaacgc atggagcgc 
      cttgcctct gaattcgaa aacggcatc ggaaagaaa 
      ctccaacgc tacttatgg ctaaaatct tggtggtca 
      agcaactac gtttccgac tggtgggaa gaatacgtt 
      tatttacgc ggccgttct cccctcatg atcaattcg 
      aatttctac ggaattgat gcaattttc atgacaccg 
      acgtccatc caagccgct agagcagcc tcggtcata 
      aattcctgc ctcgtaatg cggagattg gtcgaacga 
      caagagctc gaaccgatg atgattcaa ggagttgtt 
      ccgttgtgt tcctggcag tatgagaga attttcaac 
      acgactcga attccggga atggaaacg gacaagatc 
      cagcattgg caggattcg aatcatatt gtggtgtat 
      cataaaggg agatatttc aaagttgtg atttacaaa 
      gggaggatt ttgaaaccg tgcgaaatt caagtgcaa 
      attgagcaa attttgaat gataagtct gagcccttg 
      ccaggggag gagaaattg gcggctttg acggctgga 
      gaaaggaca cattgggcg aatgttaga aggatgttt 
      ttcaccaaa ggggtgaat aaaacttcg ttggatatt 
      attgaaaaa gcggcgttt gtggtcgct ttggacgac 
      attccctac gagtttgat cagaaagat ccgtccaag 
      ttggataac tacggtaaa atcctcctc catggcaaa 
      ggttacgac cggtggttc gacaaatcc ttcactttg 
      tgtgttggc accaatgga aggattggg ttcaatgct 
      gagcattcg tgggctgac gcagcagtg atttcccat 
      gtatgggaa tgggtggta acgacccag atccacgag 
      acccgatat gacaaaacc ggaaatacg cttggtaaa 
      cctgaacta acaccgcct actccgact cgcctcaac 
      tgggaaatc cggttcgag tgccaagaa gccatcgat 
      aagagtatg aaagtcgtt aaaagtctc atcgaagac 
      atagatttg cgtatttat gtccatgat cattacggc 
      aaaggtttt atcaaacag tgtcggttg agtcctgac 
      gcctaccta cagatggcg ctccagttg gcctactat 
      cgcgatgct ggtcgtttt gacctgact tatgaagcc 
      agtatgaca agacttttc cgtgaaggt cgaaccgaa 
      accgtacgc ccttgtacc attgaagct tccaattgg 
      gtcaaggcc atggaaaat ccgaaaagt accaccgag 
      gaaagggtt cagttgttg cgaacggcg tgtcaaaga 
      catcaggtc gcttatcag gacgccatg tgcggcaag 
      ggcatcgac aggcacttg ttttgcctt tacgtagtg 
      tcgaagtac ctagaggtg gactcgcca ttcctccag 
      gaggtgcta agtgagccg tggaggttg tccacctcg 
      cagacaccg cacggccaa actggcaga atggatctc 
      aacaaatat ccagagtgc atatcagca ggcggtggg 
      tttggtccg gttgcagat gacggttac ggtgtctcc 
      tacatcatc gcgggcgag gatatgttt ttcttccac 
      gtttcaaac aagcgaagc agcagtaaa actgattgc 
      caccggttc gctaaaaga atagaaaaa gcgcttgcc 
      gacatgaag gcgctctat attgattat aaatctcag 
      aatggtaac aaataa
    mRNA sequence
    • >TC002462 RA
      cgaaccgca cgtacgccc ctgtccgag tcattcact 
      ccttcgcgg ctaacttca cgccgctca tttaactgt 
      aaaatgaca gttcaaggc tgttccaac aaccgccac 
      cactctgca cttgctcca agtgtccca cattttcgc 
      actagcatt ccgcgttgt gccgtctcc gagtcttgt 
      cctctggag gctccaatt cgtggctag ttttgcaaa 
      actttgaga gaaaaacac ccccgtgcc ccccggagg 
      tatgcccca gtaaaccgc atccacgtt tgaaagttt 
      gaggagtga agaagagtc atggcggaa gcccattca 
      gctgttgct ttctcgttt tccatcacc cacgaaggc 
      tgggacgtc aacttcgac agagaagtc cttcacctg 
      gtgtggcaa tccggtatt cgctcttgg aagaagcga 
      atcgccagg tttcagaat agcattcac aatggtgta 
      tatccaggc catttgtgg agtttgtgg ttgatattt 
      tccattgtc acggctgtg cattacatg gacagggcg 
      gttcccttt gatcttgct gcgaaattt ttatattta 
      tttaatgat acttccacg cgatggcaa ctcctcgcg 
      tgcttttta gcagcatta gtttattgg ttagctata 
      atattcacc cttaggtac acattaaaa ttattactt 
      atgtacaag ggttggatg tatgaagcg agaggtaaa 
      ggcactaaa acatctttg cctacgaaa atgtggttc 
      atatgtgtt agaattttt agcggctgg aatacacca 
      aaactttac agtttccaa ggctcgctg ccacgttta 
      cctctgccc tccttgcac gacaccatg agcagatat 
      ttaaggagc gttcgtccg ctcctcgac gataaaaac 
      taccaacgc atggagcgc cttgcctct gaattcgaa 
      aacggcatc ggaaagaaa ctccaacgc tacttatgg 
      ctaaaatct tggtggtca agcaactac gtttccgac 
      tggtgggaa gaatacgtt tatttacgc ggccgttct 
      cccctcatg atcaattcg aatttctac ggaattgat 
      gcaattttc atgacaccg acgtccatc caagccgct 
      agagcagcc tcggtcata aattcctgc ctcgtaatg 
      cggagattg gtcgaacga caagagctc gaaccgatg 
      atgattcaa ggagttgtt ccgttgtgt tcctggcag 
      tatgagaga attttcaac acgactcga attccggga 
      atggaaacg gacaagatc cagcattgg caggattcg 
      aatcatatt gtggtgtat cataaaggg agatatttc 
      aaagttgtg atttacaaa gggaggatt ttgaaaccg 
      tgcgaaatt caagtgcaa attgagcaa attttgaat 
      gataagtct gagcccttg ccaggggag gagaaattg 
      gcggctttg acggctgga gaaaggaca cattgggcg 
      aatgttaga aggatgttt ttcaccaaa ggggtgaat 
      aaaacttcg ttggatatt attgaaaaa gcggcgttt 
      gtggtcgct ttggacgac attccctac gagtttgat 
      cagaaagat ccgtccaag ttggataac tacggtaaa 
      atcctcctc catggcaaa ggttacgac cggtggttc 
      gacaaatcc ttcactttg tgtgttggc accaatgga 
      aggattggg ttcaatgct gagcattcg tgggctgac 
      gcagcagtg atttcccat gtatgggaa tgggtggta 
      acgacccag atccacgag acccgatat gacaaaacc 
      ggaaatacg cttggtaaa cctgaacta acaccgcct 
      actccgact cgcctcaac tgggaaatc cggttcgag 
      tgccaagaa gccatcgat aagagtatg aaagtcgtt 
      aaaagtctc atcgaagac atagatttg cgtatttat 
      gtccatgat cattacggc aaaggtttt atcaaacag 
      tgtcggttg agtcctgac gcctaccta cagatggcg 
      ctccagttg gcctactat cgcgatgct ggtcgtttt 
      gacctgact tatgaagcc agtatgaca agacttttc 
      cgtgaaggt cgaaccgaa accgtacgc ccttgtacc 
      attgaagct tccaattgg gtcaaggcc atggaaaat 
      ccgaaaagt accaccgag gaaagggtt cagttgttg 
      cgaacggcg tgtcaaaga catcaggtc gcttatcag 
      gacgccatg tgcggcaag ggcatcgac aggcacttg 
      ttttgcctt tacgtagtg tcgaagtac ctagaggtg 
      gactcgcca ttcctccag gaggtgcta agtgagccg 
      tggaggttg tccacctcg cagacaccg cacggccaa 
      actggcaga atggatctc aacaaatat ccagagtgc 
      atatcagca ggcggtggg tttggtccg gttgcagat 
      gacggttac ggtgtctcc tacatcatc gcgggcgag 
      gatatgttt ttcttccac gtttcaaac aagcgaagc 
      agcagtaaa actgattgc caccggttc gctaaaaga 
      atagaaaaa gcgcttgcc gacatgaag gcgctctat 
      attgattat aaatctcag aatggtaac aaataattt 
      tttaggatt ttttttggg atctaatta agacaatct 
      ccaaggtgg tattcggtt tttaggtta agaaactta 
      agttattgc cgattttgg cgagcatta ggcacaaaa 
      ccgtgtggt tgttgcaat ttattctgt tttttgttt 
      tttattatt tgaactctt gtttaaaat tgtaatatt 
      gttgttact attgctaat gagtgcgag tattatctc 
      tacgattgt ttagttatt atgcaagga tcaatgcaa 
      tgtttgact ttattaatc aatcagttt tttactgga 
      aaaatgatg tggacctct aatcaattg tacctacgt 
      tcttatgaa tcaataaat atttgataa caaa