Status
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Gene information
General information
TC002445 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29457311..29462168
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002445
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupation not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection



    Pupal morphology
    head position irregular - Number of animals showing the phenotype: 3

    antenna position irregular - Number of animals showing the phenotype: 3

    legs position irregular - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002445 RA
      MSTPGPSKW HPTTGYYDY NYGVGINFY QPMVDYIDQ 
      KERGGRTSY PHLPWTDEL GLSQYDPKT IKSYSNEAL 
      DRVARQTEA SAKEKLRDF RATTKSSFM LSKCVSAAS 
      ITEKVNVKN RKKKEIVRQ IKTLKSKMA DDLGYDHRK 
      EDRDVELVL KGSQKYLRG RSAKGIEAF LQSESYKNI 
      AQGVEFDLA NTRNIENRR FVDTQQFTK HLKVMDKRL 
      LNQLEEKCV EPLDALSRE LKGFDKRTA EYFFDTSKA 
      TSIEIEQLN ARVVEAETR LKTEVQRIK KKLQIQITE 
      LEMSLDAAN KTNIDLQKT IKKQSLQLT ELTAHYDEV 
      QRQLQVTLD QLSVSQRKV QALTAEAEE IRGNYESAL 
      RGKRAAEQA CEEAGTRIN ELTTINVNL ASAKSKIEQ 
      ELSTIVADY DEITKELRI SDERYQRVQ TELKHTVEI 
      LHEEQERVV KIEAIKKSL EIEVKNLSV RLEEVEANA 
      IVGGKRIIS KLEARIRDI EAELDEEKR RHAETIKIL 
      RKKERTVKE VMIQCEEDQ KNIALLTES LEKANGKVA 
      LFKRQLQEQ EGVSQQSVT RVRRFQREL EAAEDRADT 
      AESSLSLIR AKHRTFVTT SSVPGSQVY LVQETRSSE 
      QI
    CDS sequence
    • >TC002445 RA
      atgtctact cccggtccc tccaaatgg cacccaacg 
      acaggctac tacgactac aattacggt gtcggaatc 
      aacttctac caaccaatg gtcgattac atcgaccag 
      aaggagcga ggtggcagg acaagctac ccccacctc 
      ccatggacc gacgaactc ggcctgagc caatacgat 
      cccaagaca attaaaagc tactcaaac gaagcactt 
      gaccgagtt gccagacaa accgaagcc agtgccaag 
      gaaaaacta cgtgatttc agagctacg accaaaagc 
      tccttcatg ttgagcaag tgtgtgtcc gccgccagc 
      atcacagag aaagtgaac gtgaaaaac aggaagaaa 
      aaggaaatc gtaagacag attaaaacc ctcaagtcg 
      aaaatggcc gacgatttg gggtacgac catcgaaag 
      gaggaccgg gacgtggag ctcgtgctc aaaggctcg 
      caaaagtac ctcagagga cgctcagct aagggcatc 
      gaagcgttc cttcaatcc gaaagttac aaaaacatc 
      gctcaaggg gttgagttc gatttggca aacacccga 
      aatatcgaa aatcgacga tttgtggac acgcaacag 
      ttcaccaag cacttgaaa gtcatggat aagaggctc 
      ttgaaccaa ctggaggaa aaatgcgtc gagcctttg 
      gacgcgcta agtcgggaa cttaaaggt ttcgacaag 
      cgaaccgcc gaatacttc ttcgacacc agcaaggcc 
      accagcatc gaaatcgag caactcaac gcccgcgtc 
      gtcgaagcc gagaccagg ctcaagacc gaagtccaa 
      cgcatcaag aagaagctc caaatccaa atcaccgaa 
      ttggaaatg tccctggac gccgccaac aagaccaac 
      atcgacttg caaaagacc atcaagaaa caatccttg 
      caactgacc gagctcaca gctcactac gacgaagtc 
      caaaggcaa ttgcaagtc accctcgac cagctcagc 
      gtctcgcag aggaaagtc caagcgctc acggcggaa 
      gccgaggag atcagaggc aactacgag tcggcattg 
      cgtggcaag agggcggcg gaacaagcc tgcgaggaa 
      gccggaacc aggatcaac gaactcact accattaac 
      gttaacttg gcttcagcc aagagcaag atcgagcaa 
      gaactgtcc actattgtt gccgattac gacgagatc 
      accaaggag ttgcgcatt tcggacgaa cgctaccaa 
      cgtgtccag accgagttg aagcacacc gtcgagatc 
      ctccacgag gagcaagag cgcgtggtg aagatcgaa 
      gccatcaag aagtccctc gagatcgaa gtcaagaac 
      ttgtccgtc cgcctcgaa gaggtcgaa gccaacgcc 
      atcgtcggc ggcaagcgc atcatcagc aagctggag 
      gcccgcatc agggacatc gaggccgag ctcgacgag 
      gagaagcgc cgccacgct gaaaccatc aagatcctg 
      cgcaagaag gagcgcacc gtcaaggaa gtgatgatc 
      cagtgcgag gaggaccag aagaacatt gccttgttg 
      accgagtct ttggagaag gccaacggc aaagtcgct 
      ctcttcaag cggcaactc caggaacag gagggcgtt 
      tcgcagcag agcgtgacc cgcgtgcgc cgcttccag 
      cgggagctg gaggccgcc gaagaccgc gccgacacc 
      gccgagagc agtttgtcg cttattagg gccaaacac 
      cgcactttc gtgaccacc agctccgtg cccggctcc 
      caggtctac ctggtgcag gagacccga agcagcgag 
      cagatctaa
    mRNA sequence
    • >TC002445 RA
      attggcttg gcacttgtc tgtttcaaa catggtata 
      aaagagccc ttcgatcga cctaaatat cagtgttaa 
      ttaattttt atcgacaat gtctactcc cggtccctc 
      caaatggca cccaacgac aggctacta cgactacaa 
      ttacggtgt cggaatcaa cttctacca accaatggt 
      cgattacat cgaccagaa ggagcgagg tggcaggac 
      aagctaccc ccacctccc atggaccga cgaactcgg 
      cctgagcca atacgatcc caagacaat taaaagcta 
      ctcaaacga agcacttga ccgagttgc cagacaaac 
      cgaagccag tgccaagga aaaactacg tgatttcag 
      agctacgac caaaagctc cttcatgtt gagcaagtg 
      tgtgtccgc cgccagcat cacagagaa agtgaacgt 
      gaaaaacag gaagaaaaa ggaaatcgt aagacagat 
      taaaaccct caagtcgaa aatggccga cgatttggg 
      gtacgacca tcgaaagga ggaccggga cgtggagct 
      cgtgctcaa aggctcgca aaagtacct cagaggacg 
      ctcagctaa gggcatcga agcgttcct tcaatccga 
      aagttacaa aaacatcgc tcaaggggt tgagttcga 
      tttggcaaa cacccgaaa tatcgaaaa tcgacgatt 
      tgtggacac gcaacagtt caccaagca cttgaaagt 
      catggataa gaggctctt gaaccaact ggaggaaaa 
      atgcgtcga gcctttgga cgcgctaag tcgggaact 
      taaaggttt cgacaagcg aaccgccga atacttctt 
      cgacaccag caaggccac cagcatcga aatcgagca 
      actcaacgc ccgcgtcgt cgaagccga gaccaggct 
      caagaccga agtccaacg catcaagaa gaagctcca 
      aatccaaat caccgaatt ggaaatgtc cctggacgc 
      cgccaacaa gaccaacat cgacttgca aaagaccat 
      caagaaaca atccttgca actgaccga gctcacagc 
      tcactacga cgaagtcca aaggcaatt gcaagtcac 
      cctcgacca gctcagcgt ctcgcagag gaaagtcca 
      agcgctcac ggcggaagc cgaggagat cagaggcaa 
      ctacgagtc ggcattgcg tggcaagag ggcggcgga 
      acaagcctg cgaggaagc cggaaccag gatcaacga 
      actcactac cattaacgt taacttggc ttcagccaa 
      gagcaagat cgagcaaga actgtccac tattgttgc 
      cgattacga cgagatcac caaggagtt gcgcatttc 
      ggacgaacg ctaccaacg tgtccagac cgagttgaa 
      gcacaccgt cgagatcct ccacgagga gcaagagcg 
      cgtggtgaa gatcgaagc catcaagaa gtccctcga 
      gatcgaagt caagaactt gtccgtccg cctcgaaga 
      ggtcgaagc caacgccat cgtcggcgg caagcgcat 
      catcagcaa gctggaggc ccgcatcag ggacatcga 
      ggccgagct cgacgagga gaagcgccg ccacgctga 
      aaccatcaa gatcctgcg caagaagga gcgcaccgt 
      caaggaagt gatgatcca gtgcgagga ggaccagaa 
      gaacattgc cttgttgac cgagtcttt ggagaaggc 
      caacggcaa agtcgctct cttcaagcg gcaactcca 
      ggaacagga gggcgtttc gcagcagag cgtgacccg 
      cgtgcgccg cttccagcg ggagctgga ggccgccga 
      agaccgcgc cgacaccgc cgagagcag tttgtcgct 
      tattagggc caaacaccg cactttcgt gaccaccag 
      ctccgtgcc cggctccca ggtctacct ggtgcagga 
      gacccgaag cagcgagca gatctaact gcacagcga 
      ccgccgatt cgtctgtct gagcacagc taacgatcg 
      aagacactc gaaccggtc catgttatt catttgatt 
      catttttta ttttatttt tttccgttc gtgcacata 
      atgttgatg aaaattatt atacatata actaccact 
      ctcttttaa agattgttg ttattaagt cattgatta 
      acagaaaga aacatacac atttcacac gcaccattt 
      taaacgaac atgagcgat ttttatttg tttcgtgta 
      tcgcgacgt gtctctaat acgtaactc gagaaggct 
      gtaaggaaa tttgtaggt ttcaggacg ttaggatta 
      tcatctgtt aacttctac aaggatgca ggagtaatg 
      tactatgta agtttgaat gtactgtgt aagaataaa 
      ttgcttata tacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY