Status
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Gene information
General information
TC002432 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28969157..28972203
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002432
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00372

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellogenic egg chamber number increased - Number of animals showing the phenotype: 2
    vitellogenic egg chamber deposition delayed - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002432 RA
      MNLTLSDSL LGRQRSRQW TPLVTQQYR LRHVHQIIG 
      LNFPPIPKK GSYYFTLHL TTMSSPFYT SDKIENANP 
      KWPELDQRN LTNLSTTAL VLRVWRHVG DSPDTIVST 
      WGVNLSGLI YLGSKIAEI QPAFFHPNT VIFSIRGGF 
      FTSRHAVNE EAPKKPPFL GNINVIDVG GNKQIFRKT 
      AVQTAKSEV RGSYNINKL RKLHSLQVT IKNKGLDVQ 
      VVREKIEVK CGTNSTETN SPESTQISS PSNIRYAPQ 
      LLTMKSVNK MLEQKPTKM QRKEMEKMR NEIEIEKFR 
      SKLLSQERD KKSALIRRF KNKLKELVE LNEKTNMEL 
      MDNYYKLSR ESEQLRKMK KDMACSQEL LMTLYTQLH 
      QRRRQLLQQ LLFIYPIQQ VSEKKYTIH GIYLPNSDI 
      LSDCSDLGI SVALGYITH VLLMTSTFL QVPLRYPMT 
      HYGSRSYIT DHVSPILPD RERDFPLFT KGKEKIQFT 
      YAVYLLNKN IAQLRWLYF MHTPELKAT LPNLLTFLE 
      GRDCRETLP LVTISLGLN AEVECHRGP EVKISEEKC 
      LECPKKSRV LRSSECGPG LSEILAIPE AFMNKQITS 
      DSFKNYIAS KKTADEASA SIVIKQGVV DENTKNCDT 
      SEEAIEQGT DLLDPSVDL ADLAGNLNG DKPGDMSDP 
      EIRKMDDSQ TELLEKWMK NGTSSECCS KETCEKFPE 
      IAVQSVDSP LMARTDALR TTKSFNLVK PKPNL
    CDS sequence
    • >TC002432 RA
      atgaatctc accctgtcg gacagttta ttggggcga 
      cagaggagt cgacaatgg accccctta gtaacacaa 
      cagtaccgt ctacgtcat gtccaccaa ataatcggc 
      ttgaatttt ccgcctata ccgaagaag ggcagttac 
      tacttcacc ctccacttg accacgatg agttcgccc 
      ttctacaca agcgataaa atcgaaaac gccaaccct 
      aaatggccc gaactggac caacgcaat ttgacgaat 
      ttgtcaacc accgccttg gttttgcgc gtgtggcgc 
      cacgttggt gactccccc gacaccata gtctccacc 
      tggggggtt aacctatca gggctcatc tacttaggg 
      agtaaaata gctgaaatc caaccagct ttctttcac 
      cccaacact gtgatattt tctataagg ggcggcttt 
      ttcacgagt aggcacgcc gtcaacgag gaggccccc 
      aaaaagccc ccttttttg ggcaacatt aacgtcata 
      gacgtgggg ggcaataag cagattttc aggaagacg 
      gccgtgcaa acggccaaa agtgaggtt aggggtagt 
      tacaatatc aacaaactg aggaaattg cacagttta 
      caagtcact attaaaaat aagggcttg gacgtgcaa 
      gtggttagg gaaaaaatt gaagtcaag tgtggtacc 
      aacagtact gaaacaaac tctccagag agtacccaa 
      atttctagt ccctctaat attaggtac gcgccgcag 
      ttacttacc atgaagtcg gtcaataaa atgctggag 
      caaaaaccg accaaaatg cagagaaaa gaaatggaa 
      aagatgcga aatgaaatc gagattgaa aaattccgt 
      agcaaatta ctatcgcaa gagcgcgat aaaaaatcc 
      gccttgatt cggcgtttc aaaaacaaa ctaaaggag 
      ttggtagaa cttaacgag aaaacaaac atggaatta 
      atggataac tactacaaa ctgagccgt gagagtgaa 
      cagttgcga aaaatgaaa aaagacatg gcttgcagt 
      caggagtta ttgatgact ttgtacaca caattgcac 
      caacgtcgc agacaatta ctccaacag ttgttattc 
      atctatccg atacaacaa gtctcagaa aagaaatac 
      acaattcat gggatttac ctcccgaat tcggacatt 
      ttgtcagac tgctccgat ttgggcatt tctgtggct 
      ttgggttac atcacccac gtgttactg atgacttcc 
      acgttcttg caagtgccc ttacgttac ccgatgacg 
      cattacggg tcaaggtca tacatcacc gatcacgtg 
      tcgccgatt ttacccgac cgcgagcga gactttccc 
      ctcttcacc aaaggcaaa gaaaaaatt caattcact 
      tacgctgtc tatttatta aacaaaaac atagcgcaa 
      ttgcggtgg ctgtatttt atgcacacc cctgaattg 
      aaagcgacg ttgccgaat ctactgact ttccttgaa 
      gggcgcgac tgtagggaa accctacct ttggttacg 
      attagttta ggtttgaat gcggaagtt gagtgtcat 
      cggggaccg gaagtgaaa attagcgag gagaaatgt 
      ctcgagtgc ccgaaaaaa agtagggtt ttaaggagt 
      agtgagtgt gggccgggc ttaagcgag attttggcc 
      attccggag gctttcatg aataagcag atcacgtcg 
      gattccttc aagaattac atcgcttcg aaaaaaaca 
      gcagacgag gccagcgcg tcaatcgtc ataaaacaa 
      ggcgtcgtg gacgaaaat accaaaaac tgtgatact 
      agcgaggaa gcgattgag caagggacg gatttgctt 
      gacccctct gtagatttg gcagatttg gcgggtaat 
      ttaaacggg gacaagcca ggagacatg tccgacccg 
      gaaatccgc aaaatggac gacagtcag acggaactt 
      ttggaaaaa tggatgaaa aacggcacc tcctccgaa 
      tgttgcagc aaggaaacg tgtgaaaaa ttccccgag 
      attgcagta caaagtgtc gatagtccg ctgatggca 
      aggacggac gcattgcgt accacgaaa tcgttcaac 
      ttggttaag ccgaaaccg aacttgtga
    mRNA sequence
    • >TC002432 RA
      agacaaaat ccatgatgc gttgttgtt gatacttgt 
      cattaatta taattagtg attaattaa agtgtttac 
      aaccgtcag taccaaagt gacttaact taattattg 
      tagaaatgg taagaataa ttatttttt cattatgga 
      cgtaaactt tgacatcta gatctagac aacataacc 
      tcaaagaaa catgatatg tcaaagcaa gcagtaaat 
      tgtttcaga tgtcatgaa tctcaccct gtcggacag 
      tttattggg gcgacagag gagtcgaca atggacccc 
      cttagtaac acaacagta ccgtctacg tcatgtcca 
      ccaaataat cggcttgaa ttttccgcc tataccgaa 
      gaagggcag ttactactt caccctcca cttgaccac 
      gatgagttc gcccttcta cacaagcga taaaatcga 
      aaacgccaa ccctaaatg gcccgaact ggaccaacg 
      caatttgac gaatttgtc aaccaccgc cttggtttt 
      gcgcgtgtg gcgccacgt tggtgactc ccccgacac 
      catagtctc cacctgggg ggttaacct atcagggct 
      catctactt agggagtaa aatagctga aatccaacc 
      agctttctt tcaccccaa cactgtgat attttctat 
      aaggggcgg ctttttcac gagtaggca cgccgtcaa 
      cgaggaggc ccccaaaaa gcccccttt tttgggcaa 
      cattaacgt catagacgt ggggggcaa taagcagat 
      tttcaggaa gacggccgt gcaaacggc caaaagtga 
      ggttagggg tagttacaa tatcaacaa actgaggaa 
      attgcacag tttacaagt cactattaa aaataaggg 
      cttggacgt gcaagtggt tagggaaaa aattgaagt 
      caagtgtgg taccaacag tactgaaac aaactctcc 
      agagagtac ccaaatttc tagtccctc taatattag 
      gtacgcgcc gcagttact taccatgaa gtcggtcaa 
      taaaatgct ggagcaaaa accgaccaa aatgcagag 
      aaaagaaat ggaaaagat gcgaaatga aatcgagat 
      tgaaaaatt ccgtagcaa attactatc gcaagagcg 
      cgataaaaa atccgcctt gattcggcg tttcaaaaa 
      caaactaaa ggagttggt agaacttaa cgagaaaac 
      aaacatgga attaatgga taactacta caaactgag 
      ccgtgagag tgaacagtt gcgaaaaat gaaaaaaga 
      catggcttg cagtcagga gttattgat gactttgta 
      cacacaatt gcaccaacg tcgcagaca attactcca 
      acagttgtt attcatcta tccgataca acaagtctc 
      agaaaagaa atacacaat tcatgggat ttacctccc 
      gaattcgga cattttgtc agactgctc cgatttggg 
      catttctgt ggctttggg ttacatcac ccacgtgtt 
      actgatgac ttccacgtt cttgcaagt gcccttacg 
      ttacccgat gacgcatta cgggtcaag gtcatacat 
      caccgatca cgtgtcgcc gattttacc cgaccgcga 
      gcgagactt tcccctctt caccaaagg caaagaaaa 
      aattcaatt cacttacgc tgtctattt attaaacaa 
      aaacatagc gcaattgcg gtggctgta ttttatgca 
      cacccctga attgaaagc gacgttgcc gaatctact 
      gactttcct tgaagggcg cgactgtag ggaaaccct 
      acctttggt tacgattag tttaggttt gaatgcgga 
      agttgagtg tcatcgggg accggaagt gaaaattag 
      cgaggagaa atgtctcga gtgcccgaa aaaaagtag 
      ggttttaag gagtagtga gtgtgggcc gggcttaag 
      cgagatttt ggccattcc ggaggcttt catgaataa 
      gcagatcac gtcggattc cttcaagaa ttacatcgc 
      ttcgaaaaa aacagcaga cgaggccag cgcgtcaat 
      cgtcataaa acaaggcgt cgtggacga aaataccaa 
      aaactgtga tactagcga ggaagcgat tgagcaagg 
      gacggattt gcttgaccc ctctgtaga tttggcaga 
      tttggcggg taatttaaa cggggacaa gccaggaga 
      catgtccga cccggaaat ccgcaaaat ggacgacag 
      tcagacgga acttttgga aaaatggat gaaaaacgg 
      cacctcctc cgaatgttg cagcaagga aacgtgtga 
      aaaattccc cgagattgc agtacaaag tgtcgatag 
      tccgctgat ggcaaggac ggacgcatt gcgtaccac 
      gaaatcgtt caacttggt taagccgaa accgaactt 
      gtgattgtt ttatcgaat tatttaaga ttttattat 
      ttaatacat ccttgtttt cacttagag aagttattg 
      tttatttca aatgaattt acacaataa atatttatt 
      ccattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY