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Gene information
General information
TC002426 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28789138..28792398
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002426
    iBeetle screen iB_00371

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic head partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002426 RA
      MGKKGKDKK KGKGAEKTA AKTDKKQSN KLKKELQKL 
      GEEDIETIV SQIEKEEKK RQQVSESVI GAPTRRLNF 
      TLIPHPDKE QLILYGGEF YNGQKTFVY NDLFFYNIP 
      NNTWTVVKA PAGPPPRCS HQMVATSAN KGQLWLFGG 
      EFVSPSQAQ FYHYRDLWV YHLGAKQWE KIIAPNGPS 
      ARSGHRMVY VKKNLIVFG GFHDNLRDY KYFNDVYCF 
      NTESYKWTK LEPGGTPPA PRSACCMVP LNDGRVLIY 
      GGYSKEKIK KDVDKGHVF TDSFLLTPE KNDTTGTKW 
      KWVQVKLGG SHFSPRCSM PMTSTPNNT MAYCYGGVF 
      DVEEDDENL AGVFYNDFF SLDLEKLVW RNVTISGKN 
      KNKESKKEE IVGDVEMEE EKTVEPTTI SDDGVFKVT 
      VGPSLVGST DKTINSCEI MVFQPSPRL NCGLAIKHG 
      VLFLYGGMF EDGDKQVTF SDFYSIDLK KLDEWKVLI 
      RDDTANQEW FGSDDESND GQEEEEESE DGEEDESDD 
      SEMETDQ
    CDS sequence
    • >TC002426 RA
      atgggcaaa aaaggcaaa gacaagaag aagggcaaa 
      ggggccgag aaaaccgcc gccaaaacg gacaaaaaa 
      cagtcaaac aagctcaaa aaagagttg caaaaactt 
      ggagaagaa gacattgag actatcgtg tcccaaatc 
      gagaaagag gagaagaag cgccaacaa gtgtccgag 
      agtgtcatc ggggccccc acgcgccgc ctcaacttc 
      accctcatc cctcacccc gacaaggag cagctgatc 
      ttgtacggg ggcgagttt tacaacggc caaaaaacg 
      ttcgtctac aacgacttg ttcttctac aacattccc 
      aacaacaca tggactgtt gtcaaggcc cctgcgggg 
      cctccgccc cgctgcagc caccagatg gtggccacg 
      tcggccaat aagggccag ttgtggctg tttggaggc 
      gagtttgtg agcccctcc caggcccag ttttaccac 
      tacagggac ctgtgggtg taccacctg ggggccaaa 
      cagtgggag aagatcata gcccccaat ggccccagt 
      gcccggagc gggcaccgc atggtttat gttaaaaaa 
      aatctaatt gtctttggg gggtttcat gataatttg 
      cgggactat aaatatttc aatgatgtt tattgtttt 
      aatacggag agttataag tggacgaag ttggagccg 
      ggaggcacg ccgccggcc cccaggtcg gcctgttgc 
      atggtcccc ctgaatgat gggcgagtc ttgatttat 
      gggggctat agtaaggag aaaattaag aaagatgtg 
      gataagggg catgttttc accgattcg tttctgctg 
      acaccggaa aaaaacgac acaactggg accaaatgg 
      aagtgggtg caagtcaag ctcgggggt tcccatttt 
      tcgccccgc tgtagcatg cccatgacc tccacaccc 
      aacaatacc atggcgtat tgctatggt ggtgttttc 
      gatgttgaa gaagacgac gaaaattta gcgggggtt 
      ttctacaac gattttttt tcgctcgat ttggagaaa 
      cttgtttgg cgaaatgtt acaatttcg gggaagaac 
      aagaataaa gaaagtaaa aaagaagaa attgttggt 
      gatgttgaa atggaagaa gaaaagaca gttgagcca 
      actaccatt tcagacgat ggtgttttt aaagtcact 
      gttggcccg tctttggtt ggcagtact gataaaacc 
      attaacagt tgtgaaatt atggttttt caaccatca 
      ccacgtctt aattgtggt ttggcgatt aaacatggg 
      gttttgttc ttgtatggg ggtatgttt gaagatggg 
      gataaacag gtcactttt agtgatttt tattcgatt 
      gatttgaaa aaattggat gagtggaag gttttaatt 
      cgggatgat acagcaaat caagagtgg tttggctct 
      gatgatgag tcaaatgat ggacaagag gaggaggaa 
      gaaagtgaa gatggggaa gaagatgag tctgatgat 
      tcggaaatg gaaactgat caataa
    mRNA sequence
    • >TC002426 RA
      aatttaaac gagctggtt ttataggca accagcaat 
      acaatgatc aaaattgtt ctacgacat ccgaaaaaa 
      tgtcaggtt gtatagtaa agaccctag acttcacct 
      gttaggttg gcaacaaat acaaaatta aaattaata 
      aatcaaaca attgcatta aaacccatt acttctact 
      tcaagtctt gaattctat agtgaattc tgaaatacc 
      ggtaatgaa atttgggcc ccagatgcc ttgaaaata 
      ccccgttat tggctcaag tgacataac ctcaaaaat 
      aaaaaacgt gctctagct tggccctac cactgaaca 
      caatgggca aaaaaggca aagacaaga agaagggca 
      aaggggccg agaaaaccg ccgccaaaa cggacaaaa 
      aacagtcaa acaagctca aaaaagagt tgcaaaaac 
      ttggagaag aagacattg agactatcg tgtcccaaa 
      tcgagaaag aggagaaga agcgccaac aagtgtccg 
      agagtgtca tcggggccc ccacgcgcc gcctcaact 
      tcaccctca tccctcacc ccgacaagg agcagctga 
      tcttgtacg ggggcgagt tttacaacg gccaaaaaa 
      cgttcgtct acaacgact tgttcttct acaacattc 
      ccaacaaca catggactg ttgtcaagg cccctgcgg 
      ggcctccgc cccgctgca gccaccaga tggtggcca 
      cgtcggcca ataagggcc agttgtggc tgtttggag 
      gcgagtttg tgagcccct cccaggccc agttttacc 
      actacaggg acctgtggg tgtaccacc tgggggcca 
      aacagtggg agaagatca tagccccca atggcccca 
      gtgcccgga gcgggcacc gcatggttt atgttaaaa 
      aaaatctaa ttgtctttg gggggtttc atgataatt 
      tgcgggact ataaatatt tcaatgatg tttattgtt 
      ttaatacgg agagttata agtggacga agttggagc 
      cgggaggca cgccgccgg cccccaggt cggcctgtt 
      gcatggtcc ccctgaatg atgggcgag tcttgattt 
      atgggggct atagtaagg agaaaatta agaaagatg 
      tggataagg ggcatgttt tcaccgatt cgtttctgc 
      tgacaccgg aaaaaaacg acacaactg ggaccaaat 
      ggaagtggg tgcaagtca agctcgggg gttcccatt 
      tttcgcccc gctgtagca tgcccatga cctccacac 
      ccaacaata ccatggcgt attgctatg gtggtgttt 
      tcgatgttg aagaagacg acgaaaatt tagcggggg 
      ttttctaca acgattttt tttcgctcg atttggaga 
      aacttgttt ggcgaaatg ttacaattt cggggaaga 
      acaagaata aagaaagta aaaaagaag aaattgttg 
      gtgatgttg aaatggaag aagaaaaga cagttgagc 
      caactacca tttcagacg atggtgttt ttaaagtca 
      ctgttggcc cgtctttgg ttggcagta ctgataaaa 
      ccattaaca gttgtgaaa ttatggttt ttcaaccat 
      caccacgtc ttaattgtg gtttggcga ttaaacatg 
      gggttttgt tcttgtatg ggggtatgt ttgaagatg 
      gggataaac aggtcactt ttagtgatt tttattcga 
      ttgatttga aaaaattgg atgagtgga aggttttaa 
      ttcgggatg atacagcaa atcaagagt ggtttggct 
      ctgatgatg agtcaaatg atggacaag aggaggagg 
      aagaaagtg aagatgggg aagaagatg agtctgatg 
      attcggaaa tggaaactg atcaataaa ttaaagtgt 
      ttgtaacaa taaaattat ttctttcta ttctgctgt 
      tttttattc atttcccat ataaattaa aaatttgcg 
      tttcccatc tagtaactt tgcgcgtta agaggttgg 
      cagccctga gaccgccat gttagcagc gtcagggta 
      cacttgtgt gttttttat gttttcctt taaattgga 
      atatccttg tgtgtgtcc ttgtttggt tttgtgtta 
      ttattagtg taaagacac ttctacaat gatttcctc 
      tgattccaa cgcctcctg ttgccctga tcccgctct 
      gctcctgtt ctccaatac caggttagt tttcttttg 
      tgtgtgatc caaaatgaa aaaaaaatt atacaaaaa 
      gctgttact aaacaatta aattaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY