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Gene information
General information
TC002425 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28785128..28789057
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002425
    iBeetle screen iB_00370

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    remarks: animals lost, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection

    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002425 RA
      MANIDESRL RQWLSKYHN PDVTFRDVL NAVNHYQGL 
LPFHEYYTF TDGSTMELV NLTGTIPVI YKGNTYNIP 
ICIWLMDTH PKNAPICYV KPTADMSIK PSMFVDQNG 
KIYLPYLHD WKTHDGTSD LLGLIQVMI VTFGDQPPV 
FARPKDTDM PYPSNSFMP GGGYMPPYP PTSYPPTSG 
FGGYPPYPQ SSNLPYPSY NPYPTPYPP YTGGGYPGP 
SPAVGGTGT IKDEHIRES LLTAIEEKL MRRMKELFQ 
QNQAELETL QRTQEELKQ GKMKLNTIL SRLEKEKSD 
LDKNITLLK DKEQELDKA IERLSNEEE IDVDDAVTT 
TAPLYKQLL NAFAEEAAL EDAIYYMGE ALRCGVIDL 
DVFLRQVRT LSRKQFMLR ALMQKCRQK AGLAV
    CDS sequence
    • >TC002425 RA
      atggccaat attgacgaa agcagactg cgccagtgg 
ctctcaaag taccacaac cccgacgta acatttcgg 
gacgtcctg aacgcagtg aaccactac caggggctc 
ctccccttc cacgaatac tacactttc accgatggc 
tccaccatg gagttggtc aatttgacg ggcaccatc 
cccgtcatc tacaaaggc aacacttac aacattccc 
atttgcatc tggctgatg gacacgcac cccaaaaac 
gcccccatt tgctacgtc aaacccaca gcagacatg 
tccatcaaa ccgtcaatg tttgtggac cagaatggt 
aaaatatac ctgccgtac ctccacgac tggaagacc 
catgatggg acgtcggac cttctgggg ctcattcaa 
gtcatgatt gtgaccttc ggggaccag ccccccgtt 
tttgcacgg ccgaaagac acagacatg ccctatccc 
tccaactcg tttatgcca ggagggggt tacatgccc 
ccatacccc cctacatca tacccccca acctccggt 
tttgggggc tacccccct tacccccag tcgagtaat 
ttaccatac cctagctat aatccgtat ccgacccca 
tatccaccc tacacgggg ggtggatac cctgggcct 
tcgcccgct gttgggggc acagggacc ataaaagac 
gaacacatt agagagtct ttattgacc gcaattgag 
gagaaatta atgagacgg atgaaagaa ttgtttcaa 
caaaatcaa gccgagctt gagacccta caaaggaca 
caagaggag ctcaagcaa ggaaaaatg aaattaaac 
acgattttg agtaggctt gagaaggag aaaagcgat 
ttggataaa aatataact ttgttgaaa gacaaggaa 
caagagttg gataaagcg atcgaaagg ttgagtaat 
gaggaggag attgatgtt gatgatgct gtgacgact 
acagcgccg ctgtataaa cagttattg aatgcgttt 
gctgaagaa gctgctcta gaggatgca atttattac 
atgggggaa gccctcaga tgtggtgtt attgacttg 
gatgtgttt ctcagacaa gtgaggact ctgtccagg 
aagcagttt atgttgagg gctttgatg caaaaatgc 
cgccaaaaa gccggactt gctgtttaa
    mRNA sequence
    • >TC002425 RA
      gtcaagtga gtaatcaac aaactgctt aattaattt 
ttttgtgcc cctaattaa gacgttaca tttagataa 
ataaaattt gtggtgcaa tggccaata ttgacgaaa 
gcagactgc gccagtggc tctcaaagt accacaacc 
ccgacgtaa catttcggg acgtcctga acgcagtga 
accactacc aggggctcc tccccttcc acgaatact 
acactttca ccgatggct ccaccatgg agttggtca 
atttgacgg gcaccatcc ccgtcatct acaaaggca 
acacttaca acattccca tttgcatct ggctgatgg 
acacgcacc ccaaaaacg cccccattt gctacgtca 
aacccacag cagacatgt ccatcaaac cgtcaatgt 
ttgtggacc agaatggta aaatatacc tgccgtacc 
tccacgact ggaagaccc atgatggga cgtcggacc 
ttctggggc tcattcaag tcatgattg tgaccttcg 
gggaccagc cccccgttt ttgcacggc cgaaagaca 
cagacatgc cctatccct ccaactcgt ttatgccag 
gagggggtt acatgcccc cataccccc ctacatcat 
accccccaa cctccggtt ttgggggct acccccctt 
acccccagt cgagtaatt taccatacc ctagctata 
atccgtatc cgaccccat atccaccct acacggggg 
gtggatacc ctgggcctt cgcccgctg ttgggggca 
cagggacca taaaagacg aacacatta gagagtctt 
tattgaccg caattgagg agaaattaa tgagacgga 
tgaaagaat tgtttcaac aaaatcaag ccgagcttg 
agaccctac aaaggacac aagaggagc tcaagcaag 
gaaaaatga aattaaaca cgattttga gtaggcttg 
agaaggaga aaagcgatt tggataaaa atataactt 
tgttgaaag acaaggaac aagagttgg ataaagcga 
tcgaaaggt tgagtaatg aggaggaga ttgatgttg 
atgatgctg tgacgacta cagcgccgc tgtataaac 
agttattga atgcgtttg ctgaagaag ctgctctag 
aggatgcaa tttattaca tgggggaag ccctcagat 
gtggtgtta ttgacttgg atgtgtttc tcagacaag 
tgaggactc tgtccagga agcagttta tgttgaggg 
ctttgatgc aaaaatgcc gccaaaaag ccggacttg 
ctgtttaaa tttagtcag tttttatta taatatagt 
gttatgcag tgaattatg ttatcttat aatcattaa 
tacttatga aatatatat ttaattacc aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY