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Gene information
General information
TC002425 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28785128..28789057
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002425
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00370

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    remarks: animals lost, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002425 RA
      MANIDESRL RQWLSKYHN PDVTFRDVL NAVNHYQGL 
      LPFHEYYTF TDGSTMELV NLTGTIPVI YKGNTYNIP 
      ICIWLMDTH PKNAPICYV KPTADMSIK PSMFVDQNG 
      KIYLPYLHD WKTHDGTSD LLGLIQVMI VTFGDQPPV 
      FARPKDTDM PYPSNSFMP GGGYMPPYP PTSYPPTSG 
      FGGYPPYPQ SSNLPYPSY NPYPTPYPP YTGGGYPGP 
      SPAVGGTGT IKDEHIRES LLTAIEEKL MRRMKELFQ 
      QNQAELETL QRTQEELKQ GKMKLNTIL SRLEKEKSD 
      LDKNITLLK DKEQELDKA IERLSNEEE IDVDDAVTT 
      TAPLYKQLL NAFAEEAAL EDAIYYMGE ALRCGVIDL 
      DVFLRQVRT LSRKQFMLR ALMQKCRQK AGLAV
    CDS sequence
    • >TC002425 RA
      atggccaat attgacgaa agcagactg cgccagtgg 
      ctctcaaag taccacaac cccgacgta acatttcgg 
      gacgtcctg aacgcagtg aaccactac caggggctc 
      ctccccttc cacgaatac tacactttc accgatggc 
      tccaccatg gagttggtc aatttgacg ggcaccatc 
      cccgtcatc tacaaaggc aacacttac aacattccc 
      atttgcatc tggctgatg gacacgcac cccaaaaac 
      gcccccatt tgctacgtc aaacccaca gcagacatg 
      tccatcaaa ccgtcaatg tttgtggac cagaatggt 
      aaaatatac ctgccgtac ctccacgac tggaagacc 
      catgatggg acgtcggac cttctgggg ctcattcaa 
      gtcatgatt gtgaccttc ggggaccag ccccccgtt 
      tttgcacgg ccgaaagac acagacatg ccctatccc 
      tccaactcg tttatgcca ggagggggt tacatgccc 
      ccatacccc cctacatca tacccccca acctccggt 
      tttgggggc tacccccct tacccccag tcgagtaat 
      ttaccatac cctagctat aatccgtat ccgacccca 
      tatccaccc tacacgggg ggtggatac cctgggcct 
      tcgcccgct gttgggggc acagggacc ataaaagac 
      gaacacatt agagagtct ttattgacc gcaattgag 
      gagaaatta atgagacgg atgaaagaa ttgtttcaa 
      caaaatcaa gccgagctt gagacccta caaaggaca 
      caagaggag ctcaagcaa ggaaaaatg aaattaaac 
      acgattttg agtaggctt gagaaggag aaaagcgat 
      ttggataaa aatataact ttgttgaaa gacaaggaa 
      caagagttg gataaagcg atcgaaagg ttgagtaat 
      gaggaggag attgatgtt gatgatgct gtgacgact 
      acagcgccg ctgtataaa cagttattg aatgcgttt 
      gctgaagaa gctgctcta gaggatgca atttattac 
      atgggggaa gccctcaga tgtggtgtt attgacttg 
      gatgtgttt ctcagacaa gtgaggact ctgtccagg 
      aagcagttt atgttgagg gctttgatg caaaaatgc 
      cgccaaaaa gccggactt gctgtttaa
    mRNA sequence
    • >TC002425 RA
      gtcaagtga gtaatcaac aaactgctt aattaattt 
      ttttgtgcc cctaattaa gacgttaca tttagataa 
      ataaaattt gtggtgcaa tggccaata ttgacgaaa 
      gcagactgc gccagtggc tctcaaagt accacaacc 
      ccgacgtaa catttcggg acgtcctga acgcagtga 
      accactacc aggggctcc tccccttcc acgaatact 
      acactttca ccgatggct ccaccatgg agttggtca 
      atttgacgg gcaccatcc ccgtcatct acaaaggca 
      acacttaca acattccca tttgcatct ggctgatgg 
      acacgcacc ccaaaaacg cccccattt gctacgtca 
      aacccacag cagacatgt ccatcaaac cgtcaatgt 
      ttgtggacc agaatggta aaatatacc tgccgtacc 
      tccacgact ggaagaccc atgatggga cgtcggacc 
      ttctggggc tcattcaag tcatgattg tgaccttcg 
      gggaccagc cccccgttt ttgcacggc cgaaagaca 
      cagacatgc cctatccct ccaactcgt ttatgccag 
      gagggggtt acatgcccc cataccccc ctacatcat 
      accccccaa cctccggtt ttgggggct acccccctt 
      acccccagt cgagtaatt taccatacc ctagctata 
      atccgtatc cgaccccat atccaccct acacggggg 
      gtggatacc ctgggcctt cgcccgctg ttgggggca 
      cagggacca taaaagacg aacacatta gagagtctt 
      tattgaccg caattgagg agaaattaa tgagacgga 
      tgaaagaat tgtttcaac aaaatcaag ccgagcttg 
      agaccctac aaaggacac aagaggagc tcaagcaag 
      gaaaaatga aattaaaca cgattttga gtaggcttg 
      agaaggaga aaagcgatt tggataaaa atataactt 
      tgttgaaag acaaggaac aagagttgg ataaagcga 
      tcgaaaggt tgagtaatg aggaggaga ttgatgttg 
      atgatgctg tgacgacta cagcgccgc tgtataaac 
      agttattga atgcgtttg ctgaagaag ctgctctag 
      aggatgcaa tttattaca tgggggaag ccctcagat 
      gtggtgtta ttgacttgg atgtgtttc tcagacaag 
      tgaggactc tgtccagga agcagttta tgttgaggg 
      ctttgatgc aaaaatgcc gccaaaaag ccggacttg 
      ctgtttaaa tttagtcag tttttatta taatatagt 
      gttatgcag tgaattatg ttatcttat aatcattaa 
      tacttatga aatatatat ttaattacc aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY