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Gene information
General information
TC002420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid29: 156988..160095
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002420
    iBeetle screen iB_00369
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 9 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002420 RA
      MTDKTYPNT YNVYNRPPC AKQALDNNP NQFYSYNMP 
      INNNYNQLE AFNMMSPHK TQNVCMYPE LPTWDTRIY 
      EDTLKIPDK IEMIEITSK DLSPAASYC SNLDLLVPD 
      FYKTLGKDL NYFNNQKID YPACAQSNQ GGNTQTVKY 
      CSIQNVEYH NYDYYNYYD GKVLDQNQY FDLPGDSFQ 
      PLDQQTESN DESDIIVEE SDEDCTDYG GTKVPSGDN 
      KCIICNVSY TPLGTQFYF LTLKSPLTM SSQKPVFTK 
      IVSIVGRIS NEINYLCSE CLGLINTID HLQFKLNGF 
      NTELKMKFQ RTCQDNNVP QRPNKKRFR DNKDRRMKC 
      KLCKKIVSL RQICQKHAL KHKSRFLCE FCGKTSPTL 
      RHFKHHHQH HAIKTPKIA AFVCRNCPK SFRTRSNLT 
      EHENYCLGV LPHKCQSCD KKFPSNTKL KNHVKLKHD 
      KKFIAICSI CNIGFVKLS DYKAHKISH STDKKFQCA 
      KCDKSYKTL SNLNFHMKV HSGKLPFIC SICDKGFMR 
      KEYLEAHVN NHKGVKNFG CHVCDKKFV SQKNLDSHL 
      KYHDGSAKT RTCNICGKV MTTGFEEHL RIHNNLREF 
      ECDQCDCRF NTKGTLAKH KKRKH
    CDS sequence
    • >TC002420 RA
      atgaccgac aagacgtac cccaacacc tacaacgtg 
      tacaaccgg cccccctgc gccaagcaa gccctagac 
      aacaaccca aatcaattt tactcgtac aacatgccc 
      atcaacaac aattacaac caactggaa gccttcaac 
      atgatgtca ccccacaag acccaaaac gtgtgcatg 
      taccccgaa ctaccgacg tgggacact cgcatctac 
      gaagacacc ctgaaaatc cccgacaag atcgaaatg 
      attgaaatt acaagcaag gatttgagc cctgcggcg 
      agttattgc agcaatttg gacttacta gtgcctgac 
      ttttacaag actttgggg aaggacttg aattatttt 
      aacaatcag aaaattgat tacccggct tgtgcccaa 
      agcaatcaa gggggaaac acacaaact gtaaagtac 
      tgtagcata caaaatgta gagtaccat aattacgac 
      tattacaat tattacgat ggcaaggtt ttggaccaa 
      aaccaatac tttgacttg ccgggggac agtttccag 
      cccctcgac caacaaacg gagtcaaat gatgaaagc 
      gatataatt gtagaggag tcagatgaa gactgcact 
      gattatggg ggcactaaa gtgccctca ggagacaac 
      aaatgcatc atttgcaac gtctcgtac acaccactg 
      gggacccaa ttttatttc ctaaccctc aagagcccc 
      ctaactatg tcgtcccaa aagccagtc tttacgaaa 
      attgtcagt attgtgggg cgaatttcc aacgaaatt 
      aactacttg tgcagcgaa tgcctgggg ctcattaac 
      acaatcgat catttgcaa tttaaactc aacggtttc 
      aatacggag ttaaagatg aagtttcaa agaacatgt 
      caggacaat aatgtcccc caaagacca aacaaaaaa 
      cgcttcaga gacaacaaa gaccgtaga atgaaatgc 
      aaactgtgc aaaaaaatc gtctctttg cgccaaatt 
      tgccaaaaa cacgcctta aaacacaag tcgcgtttt 
      ttgtgcgaa ttttgcggc aaaacatcg ccaactttg 
      cgccacttc aagcatcac caccaacac cacgccatc 
      aaaaccccc aaaatcgcc gcattcgtg tgccgaaac 
      tgcccgaag agtttccgg acaaggtcg aacttgacc 
      gaacacgag aactactgt ctcggggtt ttaccccac 
      aaatgccaa tcgtgtgat aagaaattc ccctcgaac 
      acgaaactc aaaaaccac gtcaaactc aaacacgac 
      aagaaattt atcgcgatt tgctccatc tgtaacatc 
      ggattcgtc aaactttcg gattacaag gcgcacaaa 
      attagccac agcactgat aagaaattt caatgtgcg 
      aagtgcgac aagtcgtac aagactttg agtaacttg 
      aacttccat atgaaagtg cacagtggg aagttacca 
      tttatttgt tcaatttgt gataagggg tttatgcgg 
      aaggagtat ttggaggca catgttaat aaccacaag 
      ggggttaaa aacttcggg tgccacgtg tgtgataaa 
      aagttcgtg tcgcagaaa aacctcgac tcgcattta 
      aaataccat gatgggtct gccaagacc aggacttgc 
      aatatttgt ggtaaagtg atgacgacc ggttttgag 
      gaacactta cgcattcat aacaatttg agggagttt 
      gagtgtgac caatgtgac tgtaggttt aacacgaaa 
      gggacttta gccaagcat aagaaacgc aaacattaa
    mRNA sequence
    • >TC002420 RA
      aatcaatta aagtacgaa actcaaaaa aatcgtgag 
      cctggaaag gggcctcaa aatgaccga caagacgta 
      ccccaacac ctacaacgt gtacaaccg gcccccctg 
      cgccaagca agccctaga caacaaccc aaatcaatt 
      ttactcgta caacatgcc catcaacaa caattacaa 
      ccaactgga agccttcaa catgatgtc accccacaa 
      gacccaaaa cgtgtgcat gtaccccga actaccgac 
      gtgggacac tcgcatcta cgaagacac cctgaaaat 
      ccccgacaa gatcgaaat gattgaaat tacaagcaa 
      ggatttgag ccctgcggc gagttattg cagcaattt 
      ggacttact agtgcctga cttttacaa gactttggg 
      gaaggactt gaattattt taacaatca gaaaattga 
      ttacccggc ttgtgccca aagcaatca agggggaaa 
      cacacaaac tgtaaagta ctgtagcat acaaaatgt 
      agagtacca taattacga ctattacaa ttattacga 
      tggcaaggt tttggacca aaaccaata ctttgactt 
      gccggggga cagtttcca gcccctcga ccaacaaac 
      ggagtcaaa tgatgaaag cgatataat tgtagagga 
      gtcagatga agactgcac tgattatgg gggcactaa 
      agtgccctc aggagacaa caaatgcat catttgcaa 
      cgtctcgta cacaccact ggggaccca attttattt 
      cctaaccct caagagccc cctaactat gtcgtccca 
      aaagccagt ctttacgaa aattgtcag tattgtggg 
      gcgaatttc caacgaaat taactactt gtgcagcga 
      atgcctggg gctcattaa cacaatcga tcatttgca 
      atttaaact caacggttt caatacgga gttaaagat 
      gaagtttca aagaacatg tcaggacaa taatgtccc 
      ccaaagacc aaacaaaaa acgcttcag agacaacaa 
      agaccgtag aatgaaatg caaactgtg caaaaaaat 
      cgtctcttt gcgccaaat ttgccaaaa acacgcctt 
      aaaacacaa gtcgcgttt tttgtgcga attttgcgg 
      caaaacatc gccaacttt gcgccactt caagcatca 
      ccaccaaca ccacgccat caaaacccc caaaatcgc 
      cgcattcgt gtgccgaaa ctgcccgaa gagtttccg 
      gacaaggtc gaacttgac cgaacacga gaactactg 
      tctcggggt tttacccca caaatgcca atcgtgtga 
      taagaaatt cccctcgaa cacgaaact caaaaacca 
      cgtcaaact caaacacga caagaaatt tatcgcgat 
      ttgctccat ctgtaacat cggattcgt caaactttc 
      ggattacaa ggcgcacaa aattagcca cagcactga 
      taagaaatt tcaatgtgc gaagtgcga caagtcgta 
      caagacttt gagtaactt gaacttcca tatgaaagt 
      gcacagtgg gaagttacc atttatttg ttcaatttg 
      tgataaggg gtttatgcg gaaggagta tttggaggc 
      acatgttaa taaccacaa gggggttaa aaacttcgg 
      gtgccacgt gtgtgataa aaagttcgt gtcgcagaa 
      aaacctcga ctcgcattt aaaatacca tgatgggtc 
      tgccaagac caggacttg caatatttg tggtaaagt 
      gatgacgac cggttttga ggaacactt acgcattca 
      taacaattt gagggagtt tgagtgtga ccaatgtga 
      ctgtaggtt taacacgaa agggacttt agccaagca 
      taagaaacg caaacatta ataaaacga gttaagcta 
      caattgttt atttacatt tatttaact acaattcta 
      gggccatga tggggcttc gccctcctt caaaacccg 
      gccgtatag cgcgctctt gtccctgaa aagggtcaa 
      acgctcggt ctccttcct gtccaaata ccatccaaa 
      agcgaagcc aaacgcagg gaaggcaat caggtagat 
      gtctttagg gatcaagta cctcagttt cattttgtc 
      gctgttttt gcgctttgt atttttggg tgtacgaat 
      aatcggcgt atattgaac ttacgacaa agacaggga 
      gcagggtta cgatgctgt agcgactga ttatgcggc 
      caaaggcaa tggccatct ga