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Gene information
General information
TC002415 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid29: 22933..27519
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002415
    iBeetle screen iB_00368
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002415 RA
      MPTDPMSFA KDFLAGGIS AAVSKTAVA PIERVKLLL 
      QVQAASKQI AADKQYKGI IDCLVRIPK EQGFFSFWR 
      GNLANVIRY FPTQALNFA FKDVYKQMF LGGVDKNTQ 
      FWRYFAGNL ASGGAAGAT SLCFVYPLD YARTRLGAD 
      VGKGKGERQ YTGLLDCIK KTVKSDGPI GLYRGFVVS 
      VQGIIIYRA SYFGFFDTA KGMLPDPKN TPFLISFLI 
      AQCVTTVSG ITSYPFDTV RRRMMMQSG RAKADIMYK 
      NTLDCWIKI GKTEGPTAF FKGAFSNVL RGTGGALVL 
      VLYDELKAL L
    CDS sequence
    • >TC002415 RA
      atgcccact gatcccatg agtttcgcc aaggatttc 
      ctcgctggg ggcatctcg gcggctgtg tccaagaca 
      gcggtggcc cccatcgag cgcgtgaag ctcctcctc 
      caggtccaa gccgctagc aagcaaatt gccgccgac 
      aaacagtat aaagggatc atcgattgc ctggtccgt 
      atccccaaa gaacaggga ttcttcagt ttctggcgt 
      ggaaatctc gccaacgtg atccgttat ttcccaacc 
      caggcattg aacttcgcc ttcaaggat gtctacaaa 
      cagatgttc ttgggcggt gttgacaaa aacacccaa 
      ttttggagg tatttcgcc ggtaatttg gcgtcaggt 
      ggtgccgct ggtgcgaca tcactttgc ttcgtctac 
      cctctagat tacgctcgt actcgtttg ggcgccgat 
      gtcggcaaa ggcaagggc gaaaggcag tacaccggc 
      cttctggac tgcattaag aagacagtg aaatcggac 
      ggaccgatc ggtttgtac cgaggtttc gttgtctca 
      gtgcaaggt atcatcatc taccgtgcc tcctacttc 
      ggcttcttc gatactgcc aagggaatg ttgcccgat 
      cccaagaac acaccgttc ctcatctca ttccttatt 
      gcacagtgc gtaacgaca gtttctgga attacgtca 
      tatccattc gacaccgtc agaaggcgt atgatgatg 
      cagtctgga cgcgctaaa gctgatatt atgtacaag 
      aatacgttg gattgctgg atcaagatc ggcaaaacc 
      gaaggccca actgccttc ttcaaagga gcgttctct 
      aacgttctc cgtggcact ggcggagct ttggttctt 
      gtactatac gacgagctt aaagctttg ctctaa
    mRNA sequence
    • >TC002415 RA
      cctcctgat tggccaaac gtgactttg acgtcaccg 
      tgccggata tcagggctc gttctgtca aaactgcct 
      ctttcgttc ccgaacaac catccggtg ttaacgtgt 
      acgagtgta aaaaccttc aaaatgccc actgatccc 
      atgagtttc gccaaggat ttcctcgct gggggcatc 
      tcggcggct gtgtccaag acagcggtg gcccccatc 
      gagcgcgtg aagctcctc ctccaggtc caagccgct 
      agcaagcaa attgccgcc gacaaacag tataaaggg 
      atcatcgat tgcctggtc cgtatcccc aaagaacag 
      ggattcttc agtttctgg cgtggaaat ctcgccaac 
      gtgatccgt tatttccca acccaggca ttgaacttc 
      gccttcaag gatgtctac aaacagatg ttcttgggc 
      ggtgttgac aaaaacacc caattttgg aggtatttc 
      gccggtaat ttggcgtca ggtggtgcc gctggtgcg 
      acatcactt tgcttcgtc taccctcta gattacgct 
      cgtactcgt ttgggcgcc gatgtcggc aaaggcaag 
      ggcgaaagg cagtacacc ggccttctg gactgcatt 
      aagaagaca gtgaaatcg gacggaccg atcggtttg 
      taccgaggt ttcgttgtc tcagtgcaa ggtatcatc 
      atctaccgt gcctcctac ttcggcttc ttcgatact 
      gccaaggga atgttgccc gatcccaag aacacaccg 
      ttcctcatc tcattcctt attgcacag tgcgtaacg 
      acagtttct ggaattacg tcatatcca ttcgacacc 
      gtcagaagg cgtatgatg atgcagtct ggacgcgct 
      aaagctgat attatgtac aagaatacg ttggattgc 
      tggatcaag atcggcaaa accgaaggc ccaactgcc 
      ttcttcaaa ggagcgttc tctaacgtt ctccgtggc 
      actggcgga gctttggtt cttgtacta tacgacgag 
      cttaaagct ttgctctaa acagaaata gtagaatta 
      ttacggttt aaattatta attgtctca taatttatt 
      tgtttcatt ccgtggttg atgcatttt ttaggccga 
      cattccttt ttttaacac tatcaggcg caggaattt 
      acattccag caatttttt ttcgttaca cggttttaa 
      taatggcat tgtaagctg aagtattga tagccctgt 
      attttaaat cctgtatat tttaacagc cgtttacca 
      ataaacagt tgtgataag t