| General information | |||
| TC032683
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 30417234..30436029 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032683 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00367 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
abdomen anteroposterior-axis increased - Number of animals showing the phenotype: 7
- >TC032683 RA
MSALLLSIL GLSVGIPHC PMWKEIAPC TCRMDSTKL TTIHCDKMT SYDQVVRLL KGHFAPEDR VSLKISFSK LDDLPFRAF NELNISIEN LKLNHDGLG ELAGDTFDG LNRVVFFSL ADNLLGKVP EHLWKRMPG VRTVDLGRT KIKALTGSS FKDLPVQCL VLAGNSISQ MDQDSFPKE VQRLHIGRN NLKTLNKTL TNLSDLIWL FANSNELTD LEGELPLDA KKLKMIHFS NNKIEKLPQ QLKTLTYLE SLFFQYNHI QSLDGTLGK ARKLVRVVL EHNNIKTLT KEDFTENEI LESLILGHN EITSLNNSL LNLKNLNFL NMTFNHLSE FSFQEIVGL QELKSIDLS YNRIKTLIG PATNLVEWN IKLTELKLD HNEIESLDG ALSGLPELL RLNLSFNKL RRISPDDLI GLDQLRLLD VSHNYLTTL EETSKTFLP RLEELRASH NYLTILERD FHGLPVLCH ADLSNNQIV ALGRDLVSK TRCKIGHGV HEGTWDTLK IYLQDNPIL CDAXXXXXX XXXXXXXXX XXXXXXXXX XXXXXXXXX XXXXXXXNN QLKPMFKTS AYEAIQLSV PHKNNLEDS ATKSQQVDL HQNDQYRME EPKIDPVKQ EKQLTKLAS EIEELRSRV EELANQNQI LLNQSNRST ERPPNDGER KP
- >TC032683 RA
atgtcggcc ctgctgctc tccatcctg ggcctctcc gtgggcatc ccccactgc cccatgtgg aaggagatc gcgccctgc acctgccgc atggactcc accaagctc accaccatc cactgcgac aagatgacc tcctacgac caggtggtg cgcctcctc aagggccac ttcgcgccc gaggaccgc gtctcgctc aagatctcc ttcagcaag ctggacgac ctgcccttc agggccttc aacgagctc aacatctcc atcgagaac ctcaagctc aaccacgac ggactgggg gagctggcg ggagacact ttcgacgga ctcaacaga gtcgtcttc ttcagcttg gccgataac ctcctaggg aaagtaccg gagcattta tggaaaagg atgccgggg gtgaggacg gtcgatctg ggaaggacc aagatcaag gcactcacc gggtccagc ttcaaagac ctcccggtg cagtgcttg gtccttgcc gggaactcc atctcccaa atggaccaa gactccttc cccaaagaa gtccaacgg ttacacatc ggacgtaac aacctcaaa accctcaac aaaactctg accaacctt tccgacctc atctggtta tttgccaat tcaaatgag ctaacagac ctagaaggg gagttaccc cttgatgcc aaaaaactg aaaatgatc cacttttcc aacaacaag atcgagaaa cttcctcaa caactcaaa accctaaca tatctcgaa tcccttttt ttccaatac aaccacatc caaagtctt gacggcacg ttgggaaaa gcgcggaaa ctcgtccgt gttgttttg gaacacaac aatattaag acattgacc aaagaagat tttaccgaa aacgaaata ctcgaatct cttatttta ggccacaat gagatcacc tccttgaat aactcgctt ttgaatttg aaaaatttg aattttttg aacatgacg ttcaatcat cttagcgag ttttcgttt caagagatt gtgggcctt caggagttg aaaagtatc gatttgtcg tacaacaga atcaaaaca ttaatcgga ccggctact aatttggtc gaatggaac ataaaatta accgaactt aaactggac cataacgaa attgagtca ctagatggc gctttgtcg ggtctgccc gaacttttg cggctcaat ttaagcttc aataagttg cgaagaata tcacccgat gatctgatt ggtttagat cagttgagg ttgttggac gtgtctcat aattatcta acaacgttg gaagaaacg agcaaaact tttcttcca cgtttggaa gagttgaga gccagtcac aattattta acgattttg gaaagagat ttccatgga cttccagtt ttgtgtcat gctgatcta tcaaataat caaattgtt gcacttggg agggattta gtgagcaaa acgaggtgc aaaattggt catggggtg catgaggga acgtgggac acccttaaa atatattta caagacaac ccgattttg tgcgatgcc nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nccaacaac cagctcaaa cccatgttc aaaacctcc gcctacgag gccatccag ctctccgtc ccccacaaa aacaacttg gaggactcc gccaccaag agccagcaa gtggatttg caccagaac gaccagtac cggatggag gagccgaaa atcgacccc gtgaagcag gaaaagcaa ctcacgaag ttggcgtcg gagattgag gagttgagg agtcgagtg gaggagttg gccaatcag aatcagatt ctgctcaac cagtcgaac aggtcgacg gagaggccg cccaacgat ggcgagagg aagccttga
- >TC032683 RA
ttttaagaa atctagaag attctaaag ccatactaa agtccttaa aactttgct aaaaatttg attatttta aacattatt taggcagtt cattagaat ttcggtcgt aattttggc tctgtttaa tgtattttt attgtatct gaaattgtt tactgtgtt ttggaattt tcactcgaa acatttgga aaaattcag attatttta actagatct agcaacgtg aaataaatt aaacatttg ctatcaaac aaacagact aaaattggg tcatagtct aataagata tcattgcaa aattttaaa cttttatca acaaagaac aaacgttac gattttttg cctcaactt tttcgttac taaatgcca attgtatca acgtgtctc aggtctgtg aaaaatggt ggatgcgcc acaatgcag ctaaaaata aactgagtg caataaatc gcaagggtt tagtgtgtc gtttttttt aaatgtgtc aaagagaag gcggatgaa gcgtccgtt ctatctatt aataaataa aatctcgcc gagtcataa aatttattt tgtgaataa gtagaattc caacgtgat tatcattga tttactcct gtgtttacg ttcagcgac gtcgaaaat tcctaataa tttagcgcc gcaatgaga atgtataag agtttcgga cgttcgtgt gtaaaatat gtatattta actcgattt atgggttga tgtttttgt attccttga tccagaagc gatgtattt acacaattt acagcacgc tttacattt tatgagcga taatttgat cgatttgtt gtggaaaca cgacaccga taacaatat ttagatggc ataaaggag acacaagtc tgaggactg atctaaatt ttcaattga taactgaaa cgtaaacaa tgaatgagg tggacaagc tgggcttta tctagagtc aacaggaaa ataaaactt aatacttac gtttccatc agtcggcat cgaaaaaat cctgacaca aaaaaaccc acttaccac tgagcacta ggtagtgag cgtttgcgc gtccaaggg tttgttggg taagtgtgt gaaggtaag gtaagaagg gcctgcggc ttcggcgcc aatctaaat gaggaagtg cagtcgttc gtcgggcgc cacgtcctc ctcgcaata ataaatctc gttctggcc gaatgcatc taattatct tggtggagt tgcacacac gttgtaccc cggggacac cgctagaga tatggcggg cagtggcgc cgcgctcca ctcaagaaa atttaggcg ccaccgtca ctcagtcac acattgacc ggctcaaag ccaacacca agggcctcc tccgatgac aaccgcctt caccatgtc ggccctgct gctctccat cctgggcct ctccgtggg catccccca ctgccccat gtggaagga gatcgcgcc ctgcacctg ccgcatgga ctccaccaa gctcaccac catccactg cgacaagat gacctccta cgaccaggt ggtgcgcct cctcaaggg ccacttcgc gcccgagga ccgcgtctc gctcaagat ctccttcag caagctgga cgacctgcc cttcagggc cttcaacga gctcaacat ctccatcga gaacctcaa gctcaacca cgacggact gggggagct ggcgggaga cactttcga cggactcaa cagagtcgt cttcttcag cttggccga taacctcct agggaaagt accggagca tttatggaa aaggatgcc gggggtgag gacggtcga tctgggaag gaccaagat caaggcact caccgggtc cagcttcaa agacctccc ggtgcagtg cttggtcct tgccgggaa ctccatctc ccaaatgga ccaagactc cttccccaa agaagtcca acggttaca catcggacg taacaacct caaaaccct caacaaaac tctgaccaa cctttccga cctcatctg gttatttgc caattcaaa tgagctaac agacctaga aggggagtt accccttga tgccaaaaa actgaaaat gatccactt ttccaacaa caagatcga gaaacttcc tcaacaact caaaaccct aacatatct cgaatccct ttttttcca atacaacca catccaaag tcttgacgg cacgttggg aaaagcgcg gaaactcgt ccgtgttgt tttggaaca caacaatat taagacatt gaccaaaga agattttac cgaaaacga aatactcga atctcttat tttaggcca caatgagat cacctcctt gaataactc gcttttgaa tttgaaaaa tttgaattt tttgaacat gacgttcaa tcatcttag cgagttttc gtttcaaga gattgtggg ccttcagga gttgaaaag tatcgattt gtcgtacaa cagaatcaa aacattaat cggaccggc tactaattt ggtcgaatg gaacataaa attaaccga acttaaact ggaccataa cgaaattga gtcactaga tggcgcttt gtcgggtct gcccgaact tttgcggct caatttaag cttcaataa gttgcgaag aatatcacc cgatgatct gattggttt agatcagtt gaggttgtt ggacgtgtc tcataatta tctaacaac gttggaaga aacgagcaa aacttttct tccacgttt ggaagagtt gagagccag tcacaatta tttaacgat tttggaaag agatttcca tggacttcc agttttgtg tcatgctga tctatcaaa taatcaaat tgttgcact tgggaggga tttagtgag caaaacgag gtgcaaaat tggtcatgg ggtgcatga gggaacgtg ggacaccct taaaatata tttacaaga caacccgat tttgtgcga tgccnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnccaa caaccagct caaacccat gttcaaaac ctccgccta cgaggccat ccagctctc cgtccccca caaaaacaa cttggagga ctccgccac caagagcca gcaagtgga tttgcacca gaacgacca gtaccggat ggaggagcc gaaaatcga ccccgtgaa gcaggaaaa gcaactcac gaagttggc gtcggagat tgaggagtt gaggagtcg agtggagga gttggccaa tcagaatca gattctgct caaccagtc gaacaggtc gacggagag gccgcccaa cgatggcga gaggaagcc ttgaggggg cgagttagt gcctttgtt ttttactgt agtgtagat tctgatgca agtaactta ttgctagtc atttgtagg agaaacaat cagaaactt atgatgatg ttgaaaaca aaaggggta ggaacgggg ggcgatcaa gtattgact ttagttgtg agtacaatt tattattta gagcagtat gacaataat gagtgccat ggatttatc gaaatatgt atttttcta gtagttgga gacttttct ctcattatt atttgttag gttccaatc ctgaactca tccagtagt cactagtga tgtaaatat tttcattac taacttttc tacctttta gataaatga cgacatgct gtcatagtc ataggcaat ttgtgtgta tttatatta ttaaaaata tacgttatt ttatgat
