Status
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Gene information
General information
TC032683
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002412,TC002411
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30417234..30436029
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032683
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00367

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 7

    abdomen anteroposterior-axis increased - Number of animals showing the phenotype: 7

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032683 RA
      MSALLLSIL GLSVGIPHC PMWKEIAPC TCRMDSTKL 
      TTIHCDKMT SYDQVVRLL KGHFAPEDR VSLKISFSK 
      LDDLPFRAF NELNISIEN LKLNHDGLG ELAGDTFDG 
      LNRVVFFSL ADNLLGKVP EHLWKRMPG VRTVDLGRT 
      KIKALTGSS FKDLPVQCL VLAGNSISQ MDQDSFPKE 
      VQRLHIGRN NLKTLNKTL TNLSDLIWL FANSNELTD 
      LEGELPLDA KKLKMIHFS NNKIEKLPQ QLKTLTYLE 
      SLFFQYNHI QSLDGTLGK ARKLVRVVL EHNNIKTLT 
      KEDFTENEI LESLILGHN EITSLNNSL LNLKNLNFL 
      NMTFNHLSE FSFQEIVGL QELKSIDLS YNRIKTLIG 
      PATNLVEWN IKLTELKLD HNEIESLDG ALSGLPELL 
      RLNLSFNKL RRISPDDLI GLDQLRLLD VSHNYLTTL 
      EETSKTFLP RLEELRASH NYLTILERD FHGLPVLCH 
      ADLSNNQIV ALGRDLVSK TRCKIGHGV HEGTWDTLK 
      IYLQDNPIL CDAXXXXXX XXXXXXXXX XXXXXXXXX 
      XXXXXXXXX XXXXXXXNN QLKPMFKTS AYEAIQLSV 
      PHKNNLEDS ATKSQQVDL HQNDQYRME EPKIDPVKQ 
      EKQLTKLAS EIEELRSRV EELANQNQI LLNQSNRST 
      ERPPNDGER KP
    CDS sequence
    • >TC032683 RA
      atgtcggcc ctgctgctc tccatcctg ggcctctcc 
      gtgggcatc ccccactgc cccatgtgg aaggagatc 
      gcgccctgc acctgccgc atggactcc accaagctc 
      accaccatc cactgcgac aagatgacc tcctacgac 
      caggtggtg cgcctcctc aagggccac ttcgcgccc 
      gaggaccgc gtctcgctc aagatctcc ttcagcaag 
      ctggacgac ctgcccttc agggccttc aacgagctc 
      aacatctcc atcgagaac ctcaagctc aaccacgac 
      ggactgggg gagctggcg ggagacact ttcgacgga 
      ctcaacaga gtcgtcttc ttcagcttg gccgataac 
      ctcctaggg aaagtaccg gagcattta tggaaaagg 
      atgccgggg gtgaggacg gtcgatctg ggaaggacc 
      aagatcaag gcactcacc gggtccagc ttcaaagac 
      ctcccggtg cagtgcttg gtccttgcc gggaactcc 
      atctcccaa atggaccaa gactccttc cccaaagaa 
      gtccaacgg ttacacatc ggacgtaac aacctcaaa 
      accctcaac aaaactctg accaacctt tccgacctc 
      atctggtta tttgccaat tcaaatgag ctaacagac 
      ctagaaggg gagttaccc cttgatgcc aaaaaactg 
      aaaatgatc cacttttcc aacaacaag atcgagaaa 
      cttcctcaa caactcaaa accctaaca tatctcgaa 
      tcccttttt ttccaatac aaccacatc caaagtctt 
      gacggcacg ttgggaaaa gcgcggaaa ctcgtccgt 
      gttgttttg gaacacaac aatattaag acattgacc 
      aaagaagat tttaccgaa aacgaaata ctcgaatct 
      cttatttta ggccacaat gagatcacc tccttgaat 
      aactcgctt ttgaatttg aaaaatttg aattttttg 
      aacatgacg ttcaatcat cttagcgag ttttcgttt 
      caagagatt gtgggcctt caggagttg aaaagtatc 
      gatttgtcg tacaacaga atcaaaaca ttaatcgga 
      ccggctact aatttggtc gaatggaac ataaaatta 
      accgaactt aaactggac cataacgaa attgagtca 
      ctagatggc gctttgtcg ggtctgccc gaacttttg 
      cggctcaat ttaagcttc aataagttg cgaagaata 
      tcacccgat gatctgatt ggtttagat cagttgagg 
      ttgttggac gtgtctcat aattatcta acaacgttg 
      gaagaaacg agcaaaact tttcttcca cgtttggaa 
      gagttgaga gccagtcac aattattta acgattttg 
      gaaagagat ttccatgga cttccagtt ttgtgtcat 
      gctgatcta tcaaataat caaattgtt gcacttggg 
      agggattta gtgagcaaa acgaggtgc aaaattggt 
      catggggtg catgaggga acgtgggac acccttaaa 
      atatattta caagacaac ccgattttg tgcgatgcc 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nccaacaac cagctcaaa cccatgttc 
      aaaacctcc gcctacgag gccatccag ctctccgtc 
      ccccacaaa aacaacttg gaggactcc gccaccaag 
      agccagcaa gtggatttg caccagaac gaccagtac 
      cggatggag gagccgaaa atcgacccc gtgaagcag 
      gaaaagcaa ctcacgaag ttggcgtcg gagattgag 
      gagttgagg agtcgagtg gaggagttg gccaatcag 
      aatcagatt ctgctcaac cagtcgaac aggtcgacg 
      gagaggccg cccaacgat ggcgagagg aagccttga
    mRNA sequence
    • >TC032683 RA
      ttttaagaa atctagaag attctaaag ccatactaa 
      agtccttaa aactttgct aaaaatttg attatttta 
      aacattatt taggcagtt cattagaat ttcggtcgt 
      aattttggc tctgtttaa tgtattttt attgtatct 
      gaaattgtt tactgtgtt ttggaattt tcactcgaa 
      acatttgga aaaattcag attatttta actagatct 
      agcaacgtg aaataaatt aaacatttg ctatcaaac 
      aaacagact aaaattggg tcatagtct aataagata 
      tcattgcaa aattttaaa cttttatca acaaagaac 
      aaacgttac gattttttg cctcaactt tttcgttac 
      taaatgcca attgtatca acgtgtctc aggtctgtg 
      aaaaatggt ggatgcgcc acaatgcag ctaaaaata 
      aactgagtg caataaatc gcaagggtt tagtgtgtc 
      gtttttttt aaatgtgtc aaagagaag gcggatgaa 
      gcgtccgtt ctatctatt aataaataa aatctcgcc 
      gagtcataa aatttattt tgtgaataa gtagaattc 
      caacgtgat tatcattga tttactcct gtgtttacg 
      ttcagcgac gtcgaaaat tcctaataa tttagcgcc 
      gcaatgaga atgtataag agtttcgga cgttcgtgt 
      gtaaaatat gtatattta actcgattt atgggttga 
      tgtttttgt attccttga tccagaagc gatgtattt 
      acacaattt acagcacgc tttacattt tatgagcga 
      taatttgat cgatttgtt gtggaaaca cgacaccga 
      taacaatat ttagatggc ataaaggag acacaagtc 
      tgaggactg atctaaatt ttcaattga taactgaaa 
      cgtaaacaa tgaatgagg tggacaagc tgggcttta 
      tctagagtc aacaggaaa ataaaactt aatacttac 
      gtttccatc agtcggcat cgaaaaaat cctgacaca 
      aaaaaaccc acttaccac tgagcacta ggtagtgag 
      cgtttgcgc gtccaaggg tttgttggg taagtgtgt 
      gaaggtaag gtaagaagg gcctgcggc ttcggcgcc 
      aatctaaat gaggaagtg cagtcgttc gtcgggcgc 
      cacgtcctc ctcgcaata ataaatctc gttctggcc 
      gaatgcatc taattatct tggtggagt tgcacacac 
      gttgtaccc cggggacac cgctagaga tatggcggg 
      cagtggcgc cgcgctcca ctcaagaaa atttaggcg 
      ccaccgtca ctcagtcac acattgacc ggctcaaag 
      ccaacacca agggcctcc tccgatgac aaccgcctt 
      caccatgtc ggccctgct gctctccat cctgggcct 
      ctccgtggg catccccca ctgccccat gtggaagga 
      gatcgcgcc ctgcacctg ccgcatgga ctccaccaa 
      gctcaccac catccactg cgacaagat gacctccta 
      cgaccaggt ggtgcgcct cctcaaggg ccacttcgc 
      gcccgagga ccgcgtctc gctcaagat ctccttcag 
      caagctgga cgacctgcc cttcagggc cttcaacga 
      gctcaacat ctccatcga gaacctcaa gctcaacca 
      cgacggact gggggagct ggcgggaga cactttcga 
      cggactcaa cagagtcgt cttcttcag cttggccga 
      taacctcct agggaaagt accggagca tttatggaa 
      aaggatgcc gggggtgag gacggtcga tctgggaag 
      gaccaagat caaggcact caccgggtc cagcttcaa 
      agacctccc ggtgcagtg cttggtcct tgccgggaa 
      ctccatctc ccaaatgga ccaagactc cttccccaa 
      agaagtcca acggttaca catcggacg taacaacct 
      caaaaccct caacaaaac tctgaccaa cctttccga 
      cctcatctg gttatttgc caattcaaa tgagctaac 
      agacctaga aggggagtt accccttga tgccaaaaa 
      actgaaaat gatccactt ttccaacaa caagatcga 
      gaaacttcc tcaacaact caaaaccct aacatatct 
      cgaatccct ttttttcca atacaacca catccaaag 
      tcttgacgg cacgttggg aaaagcgcg gaaactcgt 
      ccgtgttgt tttggaaca caacaatat taagacatt 
      gaccaaaga agattttac cgaaaacga aatactcga 
      atctcttat tttaggcca caatgagat cacctcctt 
      gaataactc gcttttgaa tttgaaaaa tttgaattt 
      tttgaacat gacgttcaa tcatcttag cgagttttc 
      gtttcaaga gattgtggg ccttcagga gttgaaaag 
      tatcgattt gtcgtacaa cagaatcaa aacattaat 
      cggaccggc tactaattt ggtcgaatg gaacataaa 
      attaaccga acttaaact ggaccataa cgaaattga 
      gtcactaga tggcgcttt gtcgggtct gcccgaact 
      tttgcggct caatttaag cttcaataa gttgcgaag 
      aatatcacc cgatgatct gattggttt agatcagtt 
      gaggttgtt ggacgtgtc tcataatta tctaacaac 
      gttggaaga aacgagcaa aacttttct tccacgttt 
      ggaagagtt gagagccag tcacaatta tttaacgat 
      tttggaaag agatttcca tggacttcc agttttgtg 
      tcatgctga tctatcaaa taatcaaat tgttgcact 
      tgggaggga tttagtgag caaaacgag gtgcaaaat 
      tggtcatgg ggtgcatga gggaacgtg ggacaccct 
      taaaatata tttacaaga caacccgat tttgtgcga 
      tgccnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnccaa caaccagct caaacccat 
      gttcaaaac ctccgccta cgaggccat ccagctctc 
      cgtccccca caaaaacaa cttggagga ctccgccac 
      caagagcca gcaagtgga tttgcacca gaacgacca 
      gtaccggat ggaggagcc gaaaatcga ccccgtgaa 
      gcaggaaaa gcaactcac gaagttggc gtcggagat 
      tgaggagtt gaggagtcg agtggagga gttggccaa 
      tcagaatca gattctgct caaccagtc gaacaggtc 
      gacggagag gccgcccaa cgatggcga gaggaagcc 
      ttgaggggg cgagttagt gcctttgtt ttttactgt 
      agtgtagat tctgatgca agtaactta ttgctagtc 
      atttgtagg agaaacaat cagaaactt atgatgatg 
      ttgaaaaca aaaggggta ggaacgggg ggcgatcaa 
      gtattgact ttagttgtg agtacaatt tattattta 
      gagcagtat gacaataat gagtgccat ggatttatc 
      gaaatatgt atttttcta gtagttgga gacttttct 
      ctcattatt atttgttag gttccaatc ctgaactca 
      tccagtagt cactagtga tgtaaatat tttcattac 
      taacttttc tacctttta gataaatga cgacatgct 
      gtcatagtc ataggcaat ttgtgtgta tttatatta 
      ttaaaaata tacgttatt ttatgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY