Status
Please wait ...
Gene information
General information
TC032683
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002412,TC002411 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30417234..30436029
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032683
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00367

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection

    Pupal morphology
    oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 7

    abdomen anteroposterior-axis increased - Number of animals showing the phenotype: 7

    Adult morphology
    wings not closed - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032683 RA
      MSALLLSIL GLSVGIPHC PMWKEIAPC TCRMDSTKL 
TTIHCDKMT SYDQVVRLL KGHFAPEDR VSLKISFSK 
LDDLPFRAF NELNISIEN LKLNHDGLG ELAGDTFDG 
LNRVVFFSL ADNLLGKVP EHLWKRMPG VRTVDLGRT 
KIKALTGSS FKDLPVQCL VLAGNSISQ MDQDSFPKE 
VQRLHIGRN NLKTLNKTL TNLSDLIWL FANSNELTD 
LEGELPLDA KKLKMIHFS NNKIEKLPQ QLKTLTYLE 
SLFFQYNHI QSLDGTLGK ARKLVRVVL EHNNIKTLT 
KEDFTENEI LESLILGHN EITSLNNSL LNLKNLNFL 
NMTFNHLSE FSFQEIVGL QELKSIDLS YNRIKTLIG 
PATNLVEWN IKLTELKLD HNEIESLDG ALSGLPELL 
RLNLSFNKL RRISPDDLI GLDQLRLLD VSHNYLTTL 
EETSKTFLP RLEELRASH NYLTILERD FHGLPVLCH 
ADLSNNQIV ALGRDLVSK TRCKIGHGV HEGTWDTLK 
IYLQDNPIL CDAXXXXXX XXXXXXXXX XXXXXXXXX 
XXXXXXXXX XXXXXXXNN QLKPMFKTS AYEAIQLSV 
PHKNNLEDS ATKSQQVDL HQNDQYRME EPKIDPVKQ 
EKQLTKLAS EIEELRSRV EELANQNQI LLNQSNRST 
ERPPNDGER KP
    CDS sequence
    • >TC032683 RA
      atgtcggcc ctgctgctc tccatcctg ggcctctcc 
gtgggcatc ccccactgc cccatgtgg aaggagatc 
gcgccctgc acctgccgc atggactcc accaagctc 
accaccatc cactgcgac aagatgacc tcctacgac 
caggtggtg cgcctcctc aagggccac ttcgcgccc 
gaggaccgc gtctcgctc aagatctcc ttcagcaag 
ctggacgac ctgcccttc agggccttc aacgagctc 
aacatctcc atcgagaac ctcaagctc aaccacgac 
ggactgggg gagctggcg ggagacact ttcgacgga 
ctcaacaga gtcgtcttc ttcagcttg gccgataac 
ctcctaggg aaagtaccg gagcattta tggaaaagg 
atgccgggg gtgaggacg gtcgatctg ggaaggacc 
aagatcaag gcactcacc gggtccagc ttcaaagac 
ctcccggtg cagtgcttg gtccttgcc gggaactcc 
atctcccaa atggaccaa gactccttc cccaaagaa 
gtccaacgg ttacacatc ggacgtaac aacctcaaa 
accctcaac aaaactctg accaacctt tccgacctc 
atctggtta tttgccaat tcaaatgag ctaacagac 
ctagaaggg gagttaccc cttgatgcc aaaaaactg 
aaaatgatc cacttttcc aacaacaag atcgagaaa 
cttcctcaa caactcaaa accctaaca tatctcgaa 
tcccttttt ttccaatac aaccacatc caaagtctt 
gacggcacg ttgggaaaa gcgcggaaa ctcgtccgt 
gttgttttg gaacacaac aatattaag acattgacc 
aaagaagat tttaccgaa aacgaaata ctcgaatct 
cttatttta ggccacaat gagatcacc tccttgaat 
aactcgctt ttgaatttg aaaaatttg aattttttg 
aacatgacg ttcaatcat cttagcgag ttttcgttt 
caagagatt gtgggcctt caggagttg aaaagtatc 
gatttgtcg tacaacaga atcaaaaca ttaatcgga 
ccggctact aatttggtc gaatggaac ataaaatta 
accgaactt aaactggac cataacgaa attgagtca 
ctagatggc gctttgtcg ggtctgccc gaacttttg 
cggctcaat ttaagcttc aataagttg cgaagaata 
tcacccgat gatctgatt ggtttagat cagttgagg 
ttgttggac gtgtctcat aattatcta acaacgttg 
gaagaaacg agcaaaact tttcttcca cgtttggaa 
gagttgaga gccagtcac aattattta acgattttg 
gaaagagat ttccatgga cttccagtt ttgtgtcat 
gctgatcta tcaaataat caaattgtt gcacttggg 
agggattta gtgagcaaa acgaggtgc aaaattggt 
catggggtg catgaggga acgtgggac acccttaaa 
atatattta caagacaac ccgattttg tgcgatgcc 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nccaacaac cagctcaaa cccatgttc 
aaaacctcc gcctacgag gccatccag ctctccgtc 
ccccacaaa aacaacttg gaggactcc gccaccaag 
agccagcaa gtggatttg caccagaac gaccagtac 
cggatggag gagccgaaa atcgacccc gtgaagcag 
gaaaagcaa ctcacgaag ttggcgtcg gagattgag 
gagttgagg agtcgagtg gaggagttg gccaatcag 
aatcagatt ctgctcaac cagtcgaac aggtcgacg 
gagaggccg cccaacgat ggcgagagg aagccttga
    mRNA sequence
    • >TC032683 RA
      ttttaagaa atctagaag attctaaag ccatactaa 
agtccttaa aactttgct aaaaatttg attatttta 
aacattatt taggcagtt cattagaat ttcggtcgt 
aattttggc tctgtttaa tgtattttt attgtatct 
gaaattgtt tactgtgtt ttggaattt tcactcgaa 
acatttgga aaaattcag attatttta actagatct 
agcaacgtg aaataaatt aaacatttg ctatcaaac 
aaacagact aaaattggg tcatagtct aataagata 
tcattgcaa aattttaaa cttttatca acaaagaac 
aaacgttac gattttttg cctcaactt tttcgttac 
taaatgcca attgtatca acgtgtctc aggtctgtg 
aaaaatggt ggatgcgcc acaatgcag ctaaaaata 
aactgagtg caataaatc gcaagggtt tagtgtgtc 
gtttttttt aaatgtgtc aaagagaag gcggatgaa 
gcgtccgtt ctatctatt aataaataa aatctcgcc 
gagtcataa aatttattt tgtgaataa gtagaattc 
caacgtgat tatcattga tttactcct gtgtttacg 
ttcagcgac gtcgaaaat tcctaataa tttagcgcc 
gcaatgaga atgtataag agtttcgga cgttcgtgt 
gtaaaatat gtatattta actcgattt atgggttga 
tgtttttgt attccttga tccagaagc gatgtattt 
acacaattt acagcacgc tttacattt tatgagcga 
taatttgat cgatttgtt gtggaaaca cgacaccga 
taacaatat ttagatggc ataaaggag acacaagtc 
tgaggactg atctaaatt ttcaattga taactgaaa 
cgtaaacaa tgaatgagg tggacaagc tgggcttta 
tctagagtc aacaggaaa ataaaactt aatacttac 
gtttccatc agtcggcat cgaaaaaat cctgacaca 
aaaaaaccc acttaccac tgagcacta ggtagtgag 
cgtttgcgc gtccaaggg tttgttggg taagtgtgt 
gaaggtaag gtaagaagg gcctgcggc ttcggcgcc 
aatctaaat gaggaagtg cagtcgttc gtcgggcgc 
cacgtcctc ctcgcaata ataaatctc gttctggcc 
gaatgcatc taattatct tggtggagt tgcacacac 
gttgtaccc cggggacac cgctagaga tatggcggg 
cagtggcgc cgcgctcca ctcaagaaa atttaggcg 
ccaccgtca ctcagtcac acattgacc ggctcaaag 
ccaacacca agggcctcc tccgatgac aaccgcctt 
caccatgtc ggccctgct gctctccat cctgggcct 
ctccgtggg catccccca ctgccccat gtggaagga 
gatcgcgcc ctgcacctg ccgcatgga ctccaccaa 
gctcaccac catccactg cgacaagat gacctccta 
cgaccaggt ggtgcgcct cctcaaggg ccacttcgc 
gcccgagga ccgcgtctc gctcaagat ctccttcag 
caagctgga cgacctgcc cttcagggc cttcaacga 
gctcaacat ctccatcga gaacctcaa gctcaacca 
cgacggact gggggagct ggcgggaga cactttcga 
cggactcaa cagagtcgt cttcttcag cttggccga 
taacctcct agggaaagt accggagca tttatggaa 
aaggatgcc gggggtgag gacggtcga tctgggaag 
gaccaagat caaggcact caccgggtc cagcttcaa 
agacctccc ggtgcagtg cttggtcct tgccgggaa 
ctccatctc ccaaatgga ccaagactc cttccccaa 
agaagtcca acggttaca catcggacg taacaacct 
caaaaccct caacaaaac tctgaccaa cctttccga 
cctcatctg gttatttgc caattcaaa tgagctaac 
agacctaga aggggagtt accccttga tgccaaaaa 
actgaaaat gatccactt ttccaacaa caagatcga 
gaaacttcc tcaacaact caaaaccct aacatatct 
cgaatccct ttttttcca atacaacca catccaaag 
tcttgacgg cacgttggg aaaagcgcg gaaactcgt 
ccgtgttgt tttggaaca caacaatat taagacatt 
gaccaaaga agattttac cgaaaacga aatactcga 
atctcttat tttaggcca caatgagat cacctcctt 
gaataactc gcttttgaa tttgaaaaa tttgaattt 
tttgaacat gacgttcaa tcatcttag cgagttttc 
gtttcaaga gattgtggg ccttcagga gttgaaaag 
tatcgattt gtcgtacaa cagaatcaa aacattaat 
cggaccggc tactaattt ggtcgaatg gaacataaa 
attaaccga acttaaact ggaccataa cgaaattga 
gtcactaga tggcgcttt gtcgggtct gcccgaact 
tttgcggct caatttaag cttcaataa gttgcgaag 
aatatcacc cgatgatct gattggttt agatcagtt 
gaggttgtt ggacgtgtc tcataatta tctaacaac 
gttggaaga aacgagcaa aacttttct tccacgttt 
ggaagagtt gagagccag tcacaatta tttaacgat 
tttggaaag agatttcca tggacttcc agttttgtg 
tcatgctga tctatcaaa taatcaaat tgttgcact 
tgggaggga tttagtgag caaaacgag gtgcaaaat 
tggtcatgg ggtgcatga gggaacgtg ggacaccct 
taaaatata tttacaaga caacccgat tttgtgcga 
tgccnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
nnnnnnnnn nnnnnccaa caaccagct caaacccat 
gttcaaaac ctccgccta cgaggccat ccagctctc 
cgtccccca caaaaacaa cttggagga ctccgccac 
caagagcca gcaagtgga tttgcacca gaacgacca 
gtaccggat ggaggagcc gaaaatcga ccccgtgaa 
gcaggaaaa gcaactcac gaagttggc gtcggagat 
tgaggagtt gaggagtcg agtggagga gttggccaa 
tcagaatca gattctgct caaccagtc gaacaggtc 
gacggagag gccgcccaa cgatggcga gaggaagcc 
ttgaggggg cgagttagt gcctttgtt ttttactgt 
agtgtagat tctgatgca agtaactta ttgctagtc 
atttgtagg agaaacaat cagaaactt atgatgatg 
ttgaaaaca aaaggggta ggaacgggg ggcgatcaa 
gtattgact ttagttgtg agtacaatt tattattta 
gagcagtat gacaataat gagtgccat ggatttatc 
gaaatatgt atttttcta gtagttgga gacttttct 
ctcattatt atttgttag gttccaatc ctgaactca 
tccagtagt cactagtga tgtaaatat tttcattac 
taacttttc tacctttta gataaatga cgacatgct 
gtcatagtc ataggcaat ttgtgtgta tttatatta 
ttaaaaata tacgttatt ttatgat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY