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Gene information
General information
TC002407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid30: 170925..174472
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002407
    iBeetle screen iB_00366
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 2
    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002407 RA
      MTNLGHFIT LCATILSAH AIYEDQVGK FDWKRSFIG 
      KVKHAQFDT KRLIVTTHE NIIASLSPK TGEIQWRQL 
      MEDPQEHQP QLLHLDKDA VTVSGGKSR FYVRGWDAP 
      TGSLLWEWP LVLDRPGDS FWLLQGDLL VHVLAIKGS 
      HMEVTQFSV KTGEVRAKT RKIPETWVS GDKCVTANP 
      YFVCVFNGQ LFWLDVVNE GGRASSQPI QGGPDCNLL 
      EFDGPKPAV LLVRQNSAK IVELGGAVL PHEVAPNAV 
      SIGSSVFQL EANLDNLDK LVRVKSFDL VSGRESSVE 
      IDYPLGLGG PYIVSGQCR GPACELLLS STDHALTLV 
      RLPEGRVSW TREEALSNI ISVEFFELP VSDLEASIE 
      TEFTDNDVI TMFTRRIST QTKQLVNLV SGQFGSTRD 
      DFGLHRIIV VATAVGKLF GIDTLRNGS IVWTYRLPN 
      VKPVMLLVQ RTARHAPLP AQCVLLAQD VTTGGGVLF 
      AFDPISGES RGLDRLSYR IKQAFVAPN EDEEHLKPV 
      IVIGADDSV RVFPERGPK TVAYVYTID QSLVQGYRL 
      EPDGLKPVW NLNLGVCDL VGVSTRPVT ERVHSQGRV 
      LHDRSVHYK YVNPNLIAL ATLSEDPVH KSVLNVFLV 
      DGVTGFVVY STSHKKAKG PVHLVHSEN WLVYTFFNE 
      RFRRTEIVA TELYEGSIQ SNSTVFSSF AISQLPEVK 
      TESYILPAN PVSMSVTLT ERGITNKFL LVGTSSGSV 
      VEIPWMLLQ PRFADMPCG PEESCFPYM PEIAIPPEA 
      TINYNQTLA RIRKIAVAP AKLESTSHV LVHGLDLFY 
      TRVAPSKTF DLLKEDFDH RLIVLVLVG LVVASYVTK 
      YLAAKKALR QAWK
    CDS sequence
    • >TC002407 RA
      atgacgaat ttggggcac tttatcaca ctatgcgcc 
      acaattctc tcagcccac gcgatctac gaagaccag 
      gtgggcaaa ttcgactgg aaacgctca tttatcggc 
      aaagtaaag cacgctcaa ttcgacacc aaacgcctg 
      attgtgacc acacatgag aatatcatt gctagtctg 
      agccccaaa actggcgaa atacaatgg cgccaattg 
      atggaggac ccccaggag caccagccc cagctcctg 
      cacctggac aaggacgcg gtcacagtt tctgggggc 
      aagtcccgg ttttacgtg cgcgggtgg gacgccccc 
      actggaagc ctcctgtgg gagtggccc ctggtgctg 
      gaccgcccc ggcgactcg ttctggctg ctccaaggc 
      gacttgtta gtgcatgtt ttggcaatt aagggctcc 
      cacatggaa gtgactcag tttagtgtc aaaacgggg 
      gaagtccgg gccaagacg cgcaaaatc cccgaaacg 
      tgggtttcc ggtgacaag tgcgtaaca gccaacccc 
      tacttcgtg tgtgttttt aacggacag ttgttttgg 
      ctggatgtg gttaatgag gggggcaga gcctcgtcc 
      cagccgatt caagggggc cctgactgt aatctcctc 
      gagtttgat ggcccaaaa cccgccgta ttactcgtg 
      agacaaaat agcgctaaa atcgtggaa cttgggggc 
      gcagttttg ccgcacgaa gtggcccca aacgctgtc 
      tcgatcggt agttctgtt tttcaactt gaggcaaat 
      ttggacaat ttggataaa ttggttcgg gtgaaatcg 
      tttgatcta gtgagcggg cgtgaaagt tcggtcgag 
      atcgattat ccgttaggt ctcggagga ccatatatt 
      gtgtcgggg cagtgccgt ggccccgcc tgcgagctc 
      ctcctctcc agcactgac cacgccctc acgctagtc 
      cgcctgccc gaagggcgc gtctcctgg acgcgtgaa 
      gaagcctta agtaacata atctcagtc gaatttttc 
      gagttacca gtgtccgac cttgaggcc agtatcgag 
      accgaattt accgacaac gacgtcatc accatgttc 
      acgcgccgc atttccacc caaaccaaa cagctcgtt 
      aatctagtg agcgggcag ttcggctca actcgggac 
      gacttcggc ctacaccgt atcattgtc gtggcaacg 
      gccgtcggt aaactcttc gggattgac acgcttcgc 
      aacggatca atcgtgtgg acgtaccgc ctaccgaac 
      gtcaagcct gtgatgcta ttggtgcag cgcacggcg 
      cgacacgcc cccttgccc gcccagtgc gtgctattg 
      gcccaggac gtgacgacg ggaggcggg gttttgttc 
      gcctttgat ccgattagc ggggagtca cgcgggctg 
      gaccggttg tcctatcgg atcaagcag gcgtttgtg 
      gcgcccaat gaggacgag gagcatctc aaaccggtg 
      attgtgatt ggtgcggac gattcagtg cgggtgttc 
      cccgaaagg ggtcccaaa acagtcgct tatgtttat 
      acaatcgat caaagtctg gttcaaggt tatagactt 
      gaaccggac gggttaaaa ccggtatgg aacctaaac 
      ttgggtgtg tgtgacctt gtgggggtt agcacacgt 
      ccggtgacc gagcgggtg cattcccaa ggtcgggtc 
      ttgcacgac cggtcggtt cactacaag tatgttaac 
      cccaattta attgcgttg gcaacactg agtgaagac 
      ccggtacat aagagcgtt cttaacgtg ttcctggtt 
      gatggtgtg accggtttt gttgtctac tcgacctcg 
      cataaaaaa gccaaaggg ccggtacat ttagtacat 
      tcggaaaac tggctcgtt tatacgttt ttcaacgaa 
      cggttccga agaaccgaa atcgtggca acggaactg 
      tacgaaggc tcaatccag agtaacagt accgtattc 
      agttccttt gccataagt cagttacca gaggtcaaa 
      accgagtca tacatccta ccagctaac cccgtctcc 
      atgtcagta actctgacc gaacgtggg ataaccaac 
      aagtttttg ttggtcggg accagttct ggcagcgtt 
      gtggaaatc ccctggatg ttgctccag ccgcggttt 
      gccgacatg ccctgcggt ccagaagag agctgtttc 
      ccctacatg ccagaaatc gccataccg cccgaagcc 
      actattaac tacaaccaa actctggca aggattcgc 
      aaaatcgca gtggcccct gccaagctg gagagtaca 
      agtcatgtg ttggtccac gggttggac ttgttttat 
      acgcgagtg gcgccctct aagactttt gatttgttg 
      aaggaggat tttgatcat cggttgatt gttttggtt 
      ttggtggga ctggtggtg gcatcgtat gttaccaag 
      tatctggcg gccaagaag gcgctaaga caggcttgg 
      aagtaa
    mRNA sequence
    • >TC002407 RA
      tgacttgtc aaaaccatt gaggttatg tcatagagg 
      ttatgtgtt caaaatgac gaatttggg gcactttat 
      cacactatg cgccacaat tctctcagc ccacgcgat 
      ctacgaaga ccaggtggg caaattcga ctggaaacg 
      ctcatttat cggcaaagt aaagcacgc tcaattcga 
      caccaaacg cctgattgt gaccacaca tgagaatat 
      cattgctag tctgagccc caaaactgg cgaaataca 
      atggcgcca attgatgga ggaccccca ggagcacca 
      gccccagct cctgcacct ggacaagga cgcggtcac 
      agtttctgg gggcaagtc ccggtttta cgtgcgcgg 
      gtgggacgc ccccactgg aagcctcct gtgggagtg 
      gcccctggt gctggaccg ccccggcga ctcgttctg 
      gctgctcca aggcgactt gttagtgca tgttttggc 
      aattaaggg ctcccacat ggaagtgac tcagtttag 
      tgtcaaaac gggggaagt ccgggccaa gacgcgcaa 
      aatccccga aacgtgggt ttccggtga caagtgcgt 
      aacagccaa cccctactt cgtgtgtgt ttttaacgg 
      acagttgtt ttggctgga tgtggttaa tgagggggg 
      cagagcctc gtcccagcc gattcaagg gggccctga 
      ctgtaatct cctcgagtt tgatggccc aaaacccgc 
      cgtattact cgtgagaca aaatagcgc taaaatcgt 
      ggaacttgg gggcgcagt tttgccgca cgaagtggc 
      cccaaacgc tgtctcgat cggtagttc tgtttttca 
      acttgaggc aaatttgga caatttgga taaattggt 
      tcgggtgaa atcgtttga tctagtgag cgggcgtga 
      aagttcggt cgagatcga ttatccgtt aggtctcgg 
      aggaccata tattgtgtc ggggcagtg ccgtggccc 
      cgcctgcga gctcctcct ctccagcac tgaccacgc 
      cctcacgct agtccgcct gcccgaagg gcgcgtctc 
      ctggacgcg tgaagaagc cttaagtaa cataatctc 
      agtcgaatt tttcgagtt accagtgtc cgaccttga 
      ggccagtat cgagaccga atttaccga caacgacgt 
      catcaccat gttcacgcg ccgcatttc cacccaaac 
      caaacagct cgttaatct agtgagcgg gcagttcgg 
      ctcaactcg ggacgactt cggcctaca ccgtatcat 
      tgtcgtggc aacggccgt cggtaaact cttcgggat 
      tgacacgct tcgcaacgg atcaatcgt gtggacgta 
      ccgcctacc gaacgtcaa gcctgtgat gctattggt 
      gcagcgcac ggcgcgaca cgccccctt gcccgccca 
      gtgcgtgct attggccca ggacgtgac gacgggagg 
      cggggtttt gttcgcctt tgatccgat tagcgggga 
      gtcacgcgg gctggaccg gttgtccta tcggatcaa 
      gcaggcgtt tgtggcgcc caatgagga cgaggagca 
      tctcaaacc ggtgattgt gattggtgc ggacgattc 
      agtgcgggt gttccccga aaggggtcc caaaacagt 
      cgcttatgt ttatacaat cgatcaaag tctggttca 
      aggttatag acttgaacc ggacgggtt aaaaccggt 
      atggaacct aaacttggg tgtgtgtga ccttgtggg 
      ggttagcac acgtccggt gaccgagcg ggtgcattc 
      ccaaggtcg ggtcttgca cgaccggtc ggttcacta 
      caagtatgt taaccccaa tttaattgc gttggcaac 
      actgagtga agacccggt acataagag cgttcttaa 
      cgtgttcct ggttgatgg tgtgaccgg ttttgttgt 
      ctactcgac ctcgcataa aaaagccaa agggccggt 
      acatttagt acattcgga aaactggct cgtttatac 
      gtttttcaa cgaacggtt ccgaagaac cgaaatcgt 
      ggcaacgga actgtacga aggctcaat ccagagtaa 
      cagtaccgt attcagttc ctttgccat aagtcagtt 
      accagaggt caaaaccga gtcatacat cctaccagc 
      taaccccgt ctccatgtc agtaactct gaccgaacg 
      tgggataac caacaagtt tttgttggt cgggaccag 
      ttctggcag cgttgtgga aatcccctg gatgttgct 
      ccagccgcg gtttgccga catgccctg cggtccaga 
      agagagctg tttccccta catgccaga aatcgccat 
      accgcccga agccactat taactacaa ccaaactct 
      ggcaaggat tcgcaaaat cgcagtggc ccctgccaa 
      gctggagag tacaagtca tgtgttggt ccacgggtt 
      ggacttgtt ttatacgcg agtggcgcc ctctaagac 
      ttttgattt gttgaagga ggattttga tcatcggtt 
      gattgtttt ggttttggt gggactggt ggtggcatc 
      gtatgttac caagtatct ggcggccaa gaaggcgct 
      aagacaggc ttggaagta attaggcaa taaattaag 
      ggtgggtgt ccatggacg ggctttgga tatttttta 
      ttttttggg aggatggtt ttataccct atctggtcg 
      tgatggtag tcttgaaag tatggtgtt tttgtgctc 
      cagtttaaa agtttgaat aagatttcc gaagtattt 
      gaatgacct tgagactta aatagtata tagatccta 
      cacgagaat aacaaaaat attttaaaa ttgcagttg 
      aaggaaatg tatgatgtg caaaagaat gattatttc 
      atacatgtt tagtaaaac tgctgtttt tttaactta 
      aagtcattt aattaacca aggagcaat cataactaa 
      tgccattat tttttatgg ttttacagt gccattaat 
      tttgatatt tattatgtc tcatggtta atttttata 
      tgcatggtc attgaaaac acattcaaa atttcgatt 
      ttctctaca caaattgta gtttattta ttatattaa 
      gtaaagaaa agttgttca ttatctgcc tacttttct 
      ttacgtcta tgccacttg tttttctta tagaaaaaa 
      acataaaga tttggtaat aatttttta ccagtagaa 
      taaaaaata actaaatta aataatcta ccataggta 
      tctgtaaaa gcaaacaat ggttttagg aggaaaagt 
      gcataagtg caggctttc acgattact gtcgacatt 
      cgaggtttt aaaacagat tatatgatg aaaaaatgt 
      taaaaatcc aaaatgccg ttttatgga tattaccaa 
      tttaagttt tagtcaatg tgcaccttt gttgttctg 
      tttgttgta caatcaagt cagttgttt tgttgtttg 
      atactgtgt ctttataca gcattttgt aagataatt 
      acgttcggt acggacgtg gcgtaattt gtaattaga 
      gttgttggg tcgtgtata aagtgtaaa caaagacca 
      aataaaaag tttaaaatg ca