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Gene information
General information
TC002403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 30600217..30604974
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002403
    iBeetle screen iB_00365

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002403 RA
      MEKANELLQ RQEFELANA QKIHKEKLL ADIAEQQNK 
LDIELLKLQ QEKEIERFH LIEKVLEAE QNSDRAIQQ 
LLELNSEPL AQLLQQEKE EEERILAAA NKYNSDLRK 
TDILSDMQV LLEQELTKF QQFHENRME VSRGILEQE 
MDYDYRISE ILQNQDLHK AELLSKLQE DSELQKVAV 
GALLERGDA RSWGLLQQV KLVESQLAA LTHIEMDKK 
KLEIEENLN DLSEKRIQL SCLLMDLLD QQKARRDQL 
LSTLQNLEE FNNSDDFWL KQYQSLLEK LPEGLSEAQ 
KNMDPLLAQ ALLLNGVIN CLPFLASLM QCQCNVAKI 
TESDLKEVG IVNKSDRVH ILDAFQMYR KEQIPTDTA 
PSAPVLPIE EASAPPLEN VANLGTECV ICLDSTCEV 
IFVPCGHFC CCSQCPVTL NDCPMCRTS IERKIRIIS
    • >TC002403 RB
      MVLYSETPD LGQPYLGPE TIEDTPDTS TDNNEDQTD 
AFEAKIWKL ENIKKQKLQ DFLEMEKAN ELLQRQEFE 
LANAQKIHK EKLLADIAE QQNKLDIEL LKLQQEKEI 
ERFHLIEKV LEAEQNSDR AIQQLLELN SEPLAQLLQ 
QEKEEEERI LAAANKYNS DLRKTDILS DMQVLLEQE 
LTKFQQFHE NRMEVSRGI LEQEMDYDY RISEILQNQ 
DLHKAELLS KLQEDSELQ KVAVGALLE RGDARSWGL 
LQQVKLVES QLAALTHIE MDKKKLEIE ENLNDLSEK 
RIQLSCLLM DLLDQQKAR RDQLLSTLQ NLEEFNNSD 
DFWLKQYQS LLEKLPEGL SEAQKNMDP LLAQALLLN 
GVINCLPFL ASLMQCQCN VAKITESDL KEVGIVNKS 
DRVHILDAF QMYRKEQIP TDTAPSAPV LPIEEASAP 
PLENVANLG TECVICLDS TCEVIFVPC GHFCCCSQC 
PVTLNDCPM CRTSIERKI RIIS
    CDS sequence
    • >TC002403 RA
      atggagaaa gccaacgaa ttgttacaa cgccaagag 
tttgagtta gcaaatgca caaaaaatt cataaagaa 
aagctttta gccgatatt gcggaacaa cagaataag 
cttgatatt gagttgttg aagcttcaa caagagaag 
gaaattgaa cggtttcat ttgattgag aaagtgctc 
gaagctgaa cagaattca gaccgcgcc atccaacag 
ctgctcgaa ttgaatagt gaacctctt gcgcagctt 
ttgcagcaa gaaaaggaa gaagaggag cgaatttta 
gctgcagct aataaatac aacagcgat ttgcgtaaa 
actgatatt ttgagcgat atgcaagtt ttactagaa 
caggaattg actaaattt caacagttt cacgagaat 
cggatggaa gtttcaagg gggatttta gaacaggaa 
atggattat gattatcgc atcagtgaa atcctccaa 
aatcaggat ttgcacaaa gctgagctt ttgagcaaa 
ttgcaggaa gacagtgag ttgcaaaaa gtggcagtg 
ggggcgctt ctagaaagg ggcgacgcc cgatcatgg 
gggctgttg cagcaagtg aagttagtt gagtcacag 
ttggcagca ctgactcac atcgagatg gataaaaag 
aaacttgaa attgaggaa aatctcaat gatttatca 
gagaaaaga attcaattg tcgtgttta ttgatggac 
ttgttggac cagcagaaa gcacgaagg gatcagttg 
ttgtccact ttacaaaat ttggaagaa tttaacaac 
tcggacgat ttttggctc aaacaatac caaagtttg 
ctggaaaag cttccggaa ggtttgtca gaggctcaa 
aaaaatatg gatccgctc ctggcacaa gcactttta 
ctgaatgga gtcattaat tgtttgccg tttttggcc 
agtttgatg caatgtcaa tgcaacgtt gcaaaaatc 
acagaaagc gacctcaaa gaagtggga attgtgaat 
aaatcggac cgggtccac attttagac gcattccaa 
atgtaccga aaggagcaa ataccgacc gatacggcc 
ccctcagct ccagtgctg ccaatcgaa gaggcaagt 
gcgccacca ctcgaaaat gtcgcaaat ttggggact 
gaatgcgtc atttgtttg gactcaact tgtgaggtg 
attttcgtg ccatgtgga cacttctgt tgctgttcc 
cagtgtcct gtgaccttg aacgactgt ccaatgtgc 
aggacttca atcgaacgc aaaattaga ataatttca 
tag
    • >TC002403 RB
      atggttttg tattctgaa acacccgat ttggggcaa 
ccttattta ggccccgaa actattgaa gacactcct 
gatactagc actgataac aacgaggac caaactgat 
gcttttgag gccaaaata tggaagcta gaaaatatt 
aagaaacaa aaattacaa gatttcctc gaaatggag 
aaagccaac gaattgtta caacgccaa gagtttgag 
ttagcaaat gcacaaaaa attcataaa gaaaagctt 
ttagccgat attgcggaa caacagaat aagcttgat 
attgagttg ttgaagctt caacaagag aaggaaatt 
gaacggttt catttgatt gagaaagtg ctcgaagct 
gaacagaat tcagaccgc gccatccaa cagctgctc 
gaattgaat agtgaacct cttgcgcag cttttgcag 
caagaaaag gaagaagag gagcgaatt ttagctgca 
gctaataaa tacaacagc gatttgcgt aaaactgat 
attttgagc gatatgcaa gttttacta gaacaggaa 
ttgactaaa tttcaacag tttcacgag aatcggatg 
gaagtttca agggggatt ttagaacag gaaatggat 
tatgattat cgcatcagt gaaatcctc caaaatcag 
gatttgcac aaagctgag cttttgagc aaattgcag 
gaagacagt gagttgcaa aaagtggca gtgggggcg 
cttctagaa aggggcgac gcccgatca tgggggctg 
ttgcagcaa gtgaagtta gttgagtca cagttggca 
gcactgact cacatcgag atggataaa aagaaactt 
gaaattgag gaaaatctc aatgattta tcagagaaa 
agaattcaa ttgtcgtgt ttattgatg gacttgttg 
gaccagcag aaagcacga agggatcag ttgttgtcc 
actttacaa aatttggaa gaatttaac aactcggac 
gatttttgg ctcaaacaa taccaaagt ttgctggaa 
aagcttccg gaaggtttg tcagaggct caaaaaaat 
atggatccg ctcctggca caagcactt ttactgaat 
ggagtcatt aattgtttg ccgtttttg gccagtttg 
atgcaatgt caatgcaac gttgcaaaa atcacagaa 
agcgacctc aaagaagtg ggaattgtg aataaatcg 
gaccgggtc cacatttta gacgcattc caaatgtac 
cgaaaggag caaataccg accgatacg gccccctca 
gctccagtg ctgccaatc gaagaggca agtgcgcca 
ccactcgaa aatgtcgca aatttgggg actgaatgc 
gtcatttgt ttggactca acttgtgag gtgattttc 
gtgccatgt ggacacttc tgttgctgt tcccagtgt 
cctgtgacc ttgaacgac tgtccaatg tgcaggact 
tcaatcgaa cgcaaaatt agaataatt tcatag
    mRNA sequence
    • >TC002403 RA
      agatttggg gcaacctta tttaggccc cgaaactat 
tgaagacac tcctgatac tagcactga taacaacga 
ggaccaaac tgatgcttt tgaggccaa aatatggaa 
gctagaaaa tattaaggc atgtttgtt ttttcttat 
tacgattta ttttcgatt ataactggt ggtctgact 
ttaacaagt aattttttc tattgtctt gtataaaac 
atgtttatt aatcacacc acacgttat tttcgttcg 
tagtcagtt ttaacgctt tttttgcac ttgtgtctc 
tcatacttt tgtgttata atattctta taatttttt 
tctttcgcc aaaaatatg tttgtacga ttttagaaa 
caaaaatta caagatttc ctcgaaatg gagaaagcc 
aacgaattg ttacaacgc caagagttt gagttagca 
aatgcacaa aaaattcat aaagaaaag cttttagcc 
gatattgcg gaacaacag aataagctt gatattgag 
ttgttgaag cttcaacaa gagaaggaa attgaacgg 
tttcatttg attgagaaa gtgctcgaa gctgaacag 
aattcagac cgcgccatc caacagctg ctcgaattg 
aatagtgaa cctcttgcg cagcttttg cagcaagaa 
aaggaagaa gaggagcga attttagct gcagctaat 
aaatacaac agcgatttg cgtaaaact gatattttg 
agcgatatg caagtttta ctagaacag gaattgact 
aaatttcaa cagtttcac gagaatcgg atggaagtt 
tcaaggggg attttagaa caggaaatg gattatgat 
tatcgcatc agtgaaatc ctccaaaat caggatttg 
cacaaagct gagcttttg agcaaattg caggaagac 
agtgagttg caaaaagtg gcagtgggg gcgcttcta 
gaaaggggc gacgcccga tcatggggg ctgttgcag 
caagtgaag ttagttgag tcacagttg gcagcactg 
actcacatc gagatggat aaaaagaaa cttgaaatt 
gaggaaaat ctcaatgat ttatcagag aaaagaatt 
caattgtcg tgtttattg atggacttg ttggaccag 
cagaaagca cgaagggat cagttgttg tccacttta 
caaaatttg gaagaattt aacaactcg gacgatttt 
tggctcaaa caataccaa agtttgctg gaaaagctt 
ccggaaggt ttgtcagag gctcaaaaa aatatggat 
ccgctcctg gcacaagca cttttactg aatggagtc 
attaattgt ttgccgttt ttggccagt ttgatgcaa 
tgtcaatgc aacgttgca aaaatcaca gaaagcgac 
ctcaaagaa gtgggaatt gtgaataaa tcggaccgg 
gtccacatt ttagacgca ttccaaatg taccgaaag 
gagcaaata ccgaccgat acggccccc tcagctcca 
gtgctgcca atcgaagag gcaagtgcg ccaccactc 
gaaaatgtc gcaaatttg gggactgaa tgcgtcatt 
tgtttggac tcaacttgt gaggtgatt ttcgtgcca 
tgtggacac ttctgttgc tgttcccag tgtcctgtg 
accttgaac gactgtcca atgtgcagg acttcaatc 
gaacgcaaa attagaata atttcatag ttcgttcaa 
ctgccgtca ctttttttg ctcagtgtt gctaaagtg 
tgaattctg cacgatttg tggccttaa tctagtcag 
ttgttactt tttctcatg a
    • >TC002403 RB
      cgtagttta aacttaaaa aaaaatcca ataattttt 
taagaaaag cgtgttttc gtcaaaaaa tcaatttca 
gaatagacc aacaaattc cgaacatgg ttttgtatt 
ctgaaacac ccgatttgg ggcaacctt atttaggcc 
ccgaaacta ttgaagaca ctcctgata ctagcactg 
ataacaacg aggaccaaa ctgatgctt ttgaggcca 
aaatatgga agctagaaa atattaaga aacaaaaat 
tacaagatt tcctcgaaa tggagaaag ccaacgaat 
tgttacaac gccaagagt ttgagttag caaatgcac 
aaaaaattc ataaagaaa agcttttag ccgatattg 
cggaacaac agaataagc ttgatattg agttgttga 
agcttcaac aagagaagg aaattgaac ggtttcatt 
tgattgaga aagtgctcg aagctgaac agaattcag 
accgcgcca tccaacagc tgctcgaat tgaatagtg 
aacctcttg cgcagcttt tgcagcaag aaaaggaag 
aagaggagc gaattttag ctgcagcta ataaataca 
acagcgatt tgcgtaaaa ctgatattt tgagcgata 
tgcaagttt tactagaac aggaattga ctaaatttc 
aacagtttc acgagaatc ggatggaag tttcaaggg 
ggattttag aacaggaaa tggattatg attatcgca 
tcagtgaaa tcctccaaa atcaggatt tgcacaaag 
ctgagcttt tgagcaaat tgcaggaag acagtgagt 
tgcaaaaag tggcagtgg gggcgcttc tagaaaggg 
gcgacgccc gatcatggg ggctgttgc agcaagtga 
agttagttg agtcacagt tggcagcac tgactcaca 
tcgagatgg ataaaaaga aacttgaaa ttgaggaaa 
atctcaatg atttatcag agaaaagaa ttcaattgt 
cgtgtttat tgatggact tgttggacc agcagaaag 
cacgaaggg atcagttgt tgtccactt tacaaaatt 
tggaagaat ttaacaact cggacgatt tttggctca 
aacaatacc aaagtttgc tggaaaagc ttccggaag 
gtttgtcag aggctcaaa aaaatatgg atccgctcc 
tggcacaag cacttttac tgaatggag tcattaatt 
gtttgccgt ttttggcca gtttgatgc aatgtcaat 
gcaacgttg caaaaatca cagaaagcg acctcaaag 
aagtgggaa ttgtgaata aatcggacc gggtccaca 
ttttagacg cattccaaa tgtaccgaa aggagcaaa 
taccgaccg atacggccc cctcagctc cagtgctgc 
caatcgaag aggcaagtg cgccaccac tcgaaaatg 
tcgcaaatt tggggactg aatgcgtca tttgtttgg 
actcaactt gtgaggtga ttttcgtgc catgtggac 
acttctgtt gctgttccc agtgtcctg tgaccttga 
acgactgtc caatgtgca ggacttcaa tcgaacgca 
aaattagaa taatttcat agttcgttc aactgccgt 
cactttttt tgctcagtg ttgctaaag tgtgaattc 
tgcacgatt tgtggcctt aatctagtc agttgttac 
tttttctca tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY