| General information | |||
| TC002403 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 30600217..30604974 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC002403 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00365 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC002403 RA
MEKANELLQ RQEFELANA QKIHKEKLL ADIAEQQNK LDIELLKLQ QEKEIERFH LIEKVLEAE QNSDRAIQQ LLELNSEPL AQLLQQEKE EEERILAAA NKYNSDLRK TDILSDMQV LLEQELTKF QQFHENRME VSRGILEQE MDYDYRISE ILQNQDLHK AELLSKLQE DSELQKVAV GALLERGDA RSWGLLQQV KLVESQLAA LTHIEMDKK KLEIEENLN DLSEKRIQL SCLLMDLLD QQKARRDQL LSTLQNLEE FNNSDDFWL KQYQSLLEK LPEGLSEAQ KNMDPLLAQ ALLLNGVIN CLPFLASLM QCQCNVAKI TESDLKEVG IVNKSDRVH ILDAFQMYR KEQIPTDTA PSAPVLPIE EASAPPLEN VANLGTECV ICLDSTCEV IFVPCGHFC CCSQCPVTL NDCPMCRTS IERKIRIIS
- >TC002403 RB
MVLYSETPD LGQPYLGPE TIEDTPDTS TDNNEDQTD AFEAKIWKL ENIKKQKLQ DFLEMEKAN ELLQRQEFE LANAQKIHK EKLLADIAE QQNKLDIEL LKLQQEKEI ERFHLIEKV LEAEQNSDR AIQQLLELN SEPLAQLLQ QEKEEEERI LAAANKYNS DLRKTDILS DMQVLLEQE LTKFQQFHE NRMEVSRGI LEQEMDYDY RISEILQNQ DLHKAELLS KLQEDSELQ KVAVGALLE RGDARSWGL LQQVKLVES QLAALTHIE MDKKKLEIE ENLNDLSEK RIQLSCLLM DLLDQQKAR RDQLLSTLQ NLEEFNNSD DFWLKQYQS LLEKLPEGL SEAQKNMDP LLAQALLLN GVINCLPFL ASLMQCQCN VAKITESDL KEVGIVNKS DRVHILDAF QMYRKEQIP TDTAPSAPV LPIEEASAP PLENVANLG TECVICLDS TCEVIFVPC GHFCCCSQC PVTLNDCPM CRTSIERKI RIIS
- >TC002403 RA
atggagaaa gccaacgaa ttgttacaa cgccaagag tttgagtta gcaaatgca caaaaaatt cataaagaa aagctttta gccgatatt gcggaacaa cagaataag cttgatatt gagttgttg aagcttcaa caagagaag gaaattgaa cggtttcat ttgattgag aaagtgctc gaagctgaa cagaattca gaccgcgcc atccaacag ctgctcgaa ttgaatagt gaacctctt gcgcagctt ttgcagcaa gaaaaggaa gaagaggag cgaatttta gctgcagct aataaatac aacagcgat ttgcgtaaa actgatatt ttgagcgat atgcaagtt ttactagaa caggaattg actaaattt caacagttt cacgagaat cggatggaa gtttcaagg gggatttta gaacaggaa atggattat gattatcgc atcagtgaa atcctccaa aatcaggat ttgcacaaa gctgagctt ttgagcaaa ttgcaggaa gacagtgag ttgcaaaaa gtggcagtg ggggcgctt ctagaaagg ggcgacgcc cgatcatgg gggctgttg cagcaagtg aagttagtt gagtcacag ttggcagca ctgactcac atcgagatg gataaaaag aaacttgaa attgaggaa aatctcaat gatttatca gagaaaaga attcaattg tcgtgttta ttgatggac ttgttggac cagcagaaa gcacgaagg gatcagttg ttgtccact ttacaaaat ttggaagaa tttaacaac tcggacgat ttttggctc aaacaatac caaagtttg ctggaaaag cttccggaa ggtttgtca gaggctcaa aaaaatatg gatccgctc ctggcacaa gcactttta ctgaatgga gtcattaat tgtttgccg tttttggcc agtttgatg caatgtcaa tgcaacgtt gcaaaaatc acagaaagc gacctcaaa gaagtggga attgtgaat aaatcggac cgggtccac attttagac gcattccaa atgtaccga aaggagcaa ataccgacc gatacggcc ccctcagct ccagtgctg ccaatcgaa gaggcaagt gcgccacca ctcgaaaat gtcgcaaat ttggggact gaatgcgtc atttgtttg gactcaact tgtgaggtg attttcgtg ccatgtgga cacttctgt tgctgttcc cagtgtcct gtgaccttg aacgactgt ccaatgtgc aggacttca atcgaacgc aaaattaga ataatttca tag
- >TC002403 RB
atggttttg tattctgaa acacccgat ttggggcaa ccttattta ggccccgaa actattgaa gacactcct gatactagc actgataac aacgaggac caaactgat gcttttgag gccaaaata tggaagcta gaaaatatt aagaaacaa aaattacaa gatttcctc gaaatggag aaagccaac gaattgtta caacgccaa gagtttgag ttagcaaat gcacaaaaa attcataaa gaaaagctt ttagccgat attgcggaa caacagaat aagcttgat attgagttg ttgaagctt caacaagag aaggaaatt gaacggttt catttgatt gagaaagtg ctcgaagct gaacagaat tcagaccgc gccatccaa cagctgctc gaattgaat agtgaacct cttgcgcag cttttgcag caagaaaag gaagaagag gagcgaatt ttagctgca gctaataaa tacaacagc gatttgcgt aaaactgat attttgagc gatatgcaa gttttacta gaacaggaa ttgactaaa tttcaacag tttcacgag aatcggatg gaagtttca agggggatt ttagaacag gaaatggat tatgattat cgcatcagt gaaatcctc caaaatcag gatttgcac aaagctgag cttttgagc aaattgcag gaagacagt gagttgcaa aaagtggca gtgggggcg cttctagaa aggggcgac gcccgatca tgggggctg ttgcagcaa gtgaagtta gttgagtca cagttggca gcactgact cacatcgag atggataaa aagaaactt gaaattgag gaaaatctc aatgattta tcagagaaa agaattcaa ttgtcgtgt ttattgatg gacttgttg gaccagcag aaagcacga agggatcag ttgttgtcc actttacaa aatttggaa gaatttaac aactcggac gatttttgg ctcaaacaa taccaaagt ttgctggaa aagcttccg gaaggtttg tcagaggct caaaaaaat atggatccg ctcctggca caagcactt ttactgaat ggagtcatt aattgtttg ccgtttttg gccagtttg atgcaatgt caatgcaac gttgcaaaa atcacagaa agcgacctc aaagaagtg ggaattgtg aataaatcg gaccgggtc cacatttta gacgcattc caaatgtac cgaaaggag caaataccg accgatacg gccccctca gctccagtg ctgccaatc gaagaggca agtgcgcca ccactcgaa aatgtcgca aatttgggg actgaatgc gtcatttgt ttggactca acttgtgag gtgattttc gtgccatgt ggacacttc tgttgctgt tcccagtgt cctgtgacc ttgaacgac tgtccaatg tgcaggact tcaatcgaa cgcaaaatt agaataatt tcatag
- >TC002403 RA
agatttggg gcaacctta tttaggccc cgaaactat tgaagacac tcctgatac tagcactga taacaacga ggaccaaac tgatgcttt tgaggccaa aatatggaa gctagaaaa tattaaggc atgtttgtt ttttcttat tacgattta ttttcgatt ataactggt ggtctgact ttaacaagt aattttttc tattgtctt gtataaaac atgtttatt aatcacacc acacgttat tttcgttcg tagtcagtt ttaacgctt tttttgcac ttgtgtctc tcatacttt tgtgttata atattctta taatttttt tctttcgcc aaaaatatg tttgtacga ttttagaaa caaaaatta caagatttc ctcgaaatg gagaaagcc aacgaattg ttacaacgc caagagttt gagttagca aatgcacaa aaaattcat aaagaaaag cttttagcc gatattgcg gaacaacag aataagctt gatattgag ttgttgaag cttcaacaa gagaaggaa attgaacgg tttcatttg attgagaaa gtgctcgaa gctgaacag aattcagac cgcgccatc caacagctg ctcgaattg aatagtgaa cctcttgcg cagcttttg cagcaagaa aaggaagaa gaggagcga attttagct gcagctaat aaatacaac agcgatttg cgtaaaact gatattttg agcgatatg caagtttta ctagaacag gaattgact aaatttcaa cagtttcac gagaatcgg atggaagtt tcaaggggg attttagaa caggaaatg gattatgat tatcgcatc agtgaaatc ctccaaaat caggatttg cacaaagct gagcttttg agcaaattg caggaagac agtgagttg caaaaagtg gcagtgggg gcgcttcta gaaaggggc gacgcccga tcatggggg ctgttgcag caagtgaag ttagttgag tcacagttg gcagcactg actcacatc gagatggat aaaaagaaa cttgaaatt gaggaaaat ctcaatgat ttatcagag aaaagaatt caattgtcg tgtttattg atggacttg ttggaccag cagaaagca cgaagggat cagttgttg tccacttta caaaatttg gaagaattt aacaactcg gacgatttt tggctcaaa caataccaa agtttgctg gaaaagctt ccggaaggt ttgtcagag gctcaaaaa aatatggat ccgctcctg gcacaagca cttttactg aatggagtc attaattgt ttgccgttt ttggccagt ttgatgcaa tgtcaatgc aacgttgca aaaatcaca gaaagcgac ctcaaagaa gtgggaatt gtgaataaa tcggaccgg gtccacatt ttagacgca ttccaaatg taccgaaag gagcaaata ccgaccgat acggccccc tcagctcca gtgctgcca atcgaagag gcaagtgcg ccaccactc gaaaatgtc gcaaatttg gggactgaa tgcgtcatt tgtttggac tcaacttgt gaggtgatt ttcgtgcca tgtggacac ttctgttgc tgttcccag tgtcctgtg accttgaac gactgtcca atgtgcagg acttcaatc gaacgcaaa attagaata atttcatag ttcgttcaa ctgccgtca ctttttttg ctcagtgtt gctaaagtg tgaattctg cacgatttg tggccttaa tctagtcag ttgttactt tttctcatg a
- >TC002403 RB
cgtagttta aacttaaaa aaaaatcca ataattttt taagaaaag cgtgttttc gtcaaaaaa tcaatttca gaatagacc aacaaattc cgaacatgg ttttgtatt ctgaaacac ccgatttgg ggcaacctt atttaggcc ccgaaacta ttgaagaca ctcctgata ctagcactg ataacaacg aggaccaaa ctgatgctt ttgaggcca aaatatgga agctagaaa atattaaga aacaaaaat tacaagatt tcctcgaaa tggagaaag ccaacgaat tgttacaac gccaagagt ttgagttag caaatgcac aaaaaattc ataaagaaa agcttttag ccgatattg cggaacaac agaataagc ttgatattg agttgttga agcttcaac aagagaagg aaattgaac ggtttcatt tgattgaga aagtgctcg aagctgaac agaattcag accgcgcca tccaacagc tgctcgaat tgaatagtg aacctcttg cgcagcttt tgcagcaag aaaaggaag aagaggagc gaattttag ctgcagcta ataaataca acagcgatt tgcgtaaaa ctgatattt tgagcgata tgcaagttt tactagaac aggaattga ctaaatttc aacagtttc acgagaatc ggatggaag tttcaaggg ggattttag aacaggaaa tggattatg attatcgca tcagtgaaa tcctccaaa atcaggatt tgcacaaag ctgagcttt tgagcaaat tgcaggaag acagtgagt tgcaaaaag tggcagtgg gggcgcttc tagaaaggg gcgacgccc gatcatggg ggctgttgc agcaagtga agttagttg agtcacagt tggcagcac tgactcaca tcgagatgg ataaaaaga aacttgaaa ttgaggaaa atctcaatg atttatcag agaaaagaa ttcaattgt cgtgtttat tgatggact tgttggacc agcagaaag cacgaaggg atcagttgt tgtccactt tacaaaatt tggaagaat ttaacaact cggacgatt tttggctca aacaatacc aaagtttgc tggaaaagc ttccggaag gtttgtcag aggctcaaa aaaatatgg atccgctcc tggcacaag cacttttac tgaatggag tcattaatt gtttgccgt ttttggcca gtttgatgc aatgtcaat gcaacgttg caaaaatca cagaaagcg acctcaaag aagtgggaa ttgtgaata aatcggacc gggtccaca ttttagacg cattccaaa tgtaccgaa aggagcaaa taccgaccg atacggccc cctcagctc cagtgctgc caatcgaag aggcaagtg cgccaccac tcgaaaatg tcgcaaatt tggggactg aatgcgtca tttgtttgg actcaactt gtgaggtga ttttcgtgc catgtggac acttctgtt gctgttccc agtgtcctg tgaccttga acgactgtc caatgtgca ggacttcaa tcgaacgca aaattagaa taatttcat agttcgttc aactgccgt cactttttt tgctcagtg ttgctaaag tgtgaattc tgcacgatt tgtggcctt aatctagtc agttgttac tttttctca tga
