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Gene information
General information
TC002403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid30: 207616..212373
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002403
    iBeetle screen iB_00365
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002403 RA
      MEKANELLQ RQEFELANA QKIHKEKLL ADIAEQQNK 
      LDIELLKLQ QEKEIERFH LIEKVLEAE QNSDRAIQQ 
      LLELNSEPL AQLLQQEKE EEERILAAA NKYNSDLRK 
      TDILSDMQV LLEQELTKF QQFHENRME VSRGILEQE 
      MDYDYRISE ILQNQDLHK AELLSKLQE DSELQKVAV 
      GALLERGDA RSWGLLQQV KLVESQLAA LTHIEMDKK 
      KLEIEENLN DLSEKRIQL SCLLMDLLD QQKARRDQL 
      LSTLQNLEE FNNSDDFWL KQYQSLLEK LPEGLSEAQ 
      KNMDPLLAQ ALLLNGVIN CLPFLASLM QCQCNVAKI 
      TESDLKEVG IVNKSDRVH ILDAFQMYR KEQIPTDTA 
      PSAPVLPIE EASAPPLEN VANLGTECV ICLDSTCEV 
      IFVPCGHFC CCSQCPVTL NDCPMCRTS IERKIRIIS
    • >TC002403 RB
      MVLYSETPD LGQPYLGPE TIEDTPDTS TDNNEDQTD 
      AFEAKIWKL ENIKKQKLQ DFLEMEKAN ELLQRQEFE 
      LANAQKIHK EKLLADIAE QQNKLDIEL LKLQQEKEI 
      ERFHLIEKV LEAEQNSDR AIQQLLELN SEPLAQLLQ 
      QEKEEEERI LAAANKYNS DLRKTDILS DMQVLLEQE 
      LTKFQQFHE NRMEVSRGI LEQEMDYDY RISEILQNQ 
      DLHKAELLS KLQEDSELQ KVAVGALLE RGDARSWGL 
      LQQVKLVES QLAALTHIE MDKKKLEIE ENLNDLSEK 
      RIQLSCLLM DLLDQQKAR RDQLLSTLQ NLEEFNNSD 
      DFWLKQYQS LLEKLPEGL SEAQKNMDP LLAQALLLN 
      GVINCLPFL ASLMQCQCN VAKITESDL KEVGIVNKS 
      DRVHILDAF QMYRKEQIP TDTAPSAPV LPIEEASAP 
      PLENVANLG TECVICLDS TCEVIFVPC GHFCCCSQC 
      PVTLNDCPM CRTSIERKI RIIS
    CDS sequence
    • >TC002403 RA
      atggagaaa gccaacgaa ttgttacaa cgccaagag 
      tttgagtta gcaaatgca caaaaaatt cataaagaa 
      aagctttta gccgatatt gcggaacaa cagaataag 
      cttgatatt gagttgttg aagcttcaa caagagaag 
      gaaattgaa cggtttcat ttgattgag aaagtgctc 
      gaagctgaa cagaattca gaccgcgcc atccaacag 
      ctgctcgaa ttgaatagt gaacctctt gcgcagctt 
      ttgcagcaa gaaaaggaa gaagaggag cgaatttta 
      gctgcagct aataaatac aacagcgat ttgcgtaaa 
      actgatatt ttgagcgat atgcaagtt ttactagaa 
      caggaattg actaaattt caacagttt cacgagaat 
      cggatggaa gtttcaagg gggatttta gaacaggaa 
      atggattat gattatcgc atcagtgaa atcctccaa 
      aatcaggat ttgcacaaa gctgagctt ttgagcaaa 
      ttgcaggaa gacagtgag ttgcaaaaa gtggcagtg 
      ggggcgctt ctagaaagg ggcgacgcc cgatcatgg 
      gggctgttg cagcaagtg aagttagtt gagtcacag 
      ttggcagca ctgactcac atcgagatg gataaaaag 
      aaacttgaa attgaggaa aatctcaat gatttatca 
      gagaaaaga attcaattg tcgtgttta ttgatggac 
      ttgttggac cagcagaaa gcacgaagg gatcagttg 
      ttgtccact ttacaaaat ttggaagaa tttaacaac 
      tcggacgat ttttggctc aaacaatac caaagtttg 
      ctggaaaag cttccggaa ggtttgtca gaggctcaa 
      aaaaatatg gatccgctc ctggcacaa gcactttta 
      ctgaatgga gtcattaat tgtttgccg tttttggcc 
      agtttgatg caatgtcaa tgcaacgtt gcaaaaatc 
      acagaaagc gacctcaaa gaagtggga attgtgaat 
      aaatcggac cgggtccac attttagac gcattccaa 
      atgtaccga aaggagcaa ataccgacc gatacggcc 
      ccctcagct ccagtgctg ccaatcgaa gaggcaagt 
      gcgccacca ctcgaaaat gtcgcaaat ttggggact 
      gaatgcgtc atttgtttg gactcaact tgtgaggtg 
      attttcgtg ccatgtgga cacttctgt tgctgttcc 
      cagtgtcct gtgaccttg aacgactgt ccaatgtgc 
      aggacttca atcgaacgc aaaattaga ataatttca 
      tag
    • >TC002403 RB
      atggttttg tattctgaa acacccgat ttggggcaa 
      ccttattta ggccccgaa actattgaa gacactcct 
      gatactagc actgataac aacgaggac caaactgat 
      gcttttgag gccaaaata tggaagcta gaaaatatt 
      aagaaacaa aaattacaa gatttcctc gaaatggag 
      aaagccaac gaattgtta caacgccaa gagtttgag 
      ttagcaaat gcacaaaaa attcataaa gaaaagctt 
      ttagccgat attgcggaa caacagaat aagcttgat 
      attgagttg ttgaagctt caacaagag aaggaaatt 
      gaacggttt catttgatt gagaaagtg ctcgaagct 
      gaacagaat tcagaccgc gccatccaa cagctgctc 
      gaattgaat agtgaacct cttgcgcag cttttgcag 
      caagaaaag gaagaagag gagcgaatt ttagctgca 
      gctaataaa tacaacagc gatttgcgt aaaactgat 
      attttgagc gatatgcaa gttttacta gaacaggaa 
      ttgactaaa tttcaacag tttcacgag aatcggatg 
      gaagtttca agggggatt ttagaacag gaaatggat 
      tatgattat cgcatcagt gaaatcctc caaaatcag 
      gatttgcac aaagctgag cttttgagc aaattgcag 
      gaagacagt gagttgcaa aaagtggca gtgggggcg 
      cttctagaa aggggcgac gcccgatca tgggggctg 
      ttgcagcaa gtgaagtta gttgagtca cagttggca 
      gcactgact cacatcgag atggataaa aagaaactt 
      gaaattgag gaaaatctc aatgattta tcagagaaa 
      agaattcaa ttgtcgtgt ttattgatg gacttgttg 
      gaccagcag aaagcacga agggatcag ttgttgtcc 
      actttacaa aatttggaa gaatttaac aactcggac 
      gatttttgg ctcaaacaa taccaaagt ttgctggaa 
      aagcttccg gaaggtttg tcagaggct caaaaaaat 
      atggatccg ctcctggca caagcactt ttactgaat 
      ggagtcatt aattgtttg ccgtttttg gccagtttg 
      atgcaatgt caatgcaac gttgcaaaa atcacagaa 
      agcgacctc aaagaagtg ggaattgtg aataaatcg 
      gaccgggtc cacatttta gacgcattc caaatgtac 
      cgaaaggag caaataccg accgatacg gccccctca 
      gctccagtg ctgccaatc gaagaggca agtgcgcca 
      ccactcgaa aatgtcgca aatttgggg actgaatgc 
      gtcatttgt ttggactca acttgtgag gtgattttc 
      gtgccatgt ggacacttc tgttgctgt tcccagtgt 
      cctgtgacc ttgaacgac tgtccaatg tgcaggact 
      tcaatcgaa cgcaaaatt agaataatt tcatag
    mRNA sequence
    • >TC002403 RA
      agatttggg gcaacctta tttaggccc cgaaactat 
      tgaagacac tcctgatac tagcactga taacaacga 
      ggaccaaac tgatgcttt tgaggccaa aatatggaa 
      gctagaaaa tattaaggc atgtttgtt ttttcttat 
      tacgattta ttttcgatt ataactggt ggtctgact 
      ttaacaagt aattttttc tattgtctt gtataaaac 
      atgtttatt aatcacacc acacgttat tttcgttcg 
      tagtcagtt ttaacgctt tttttgcac ttgtgtctc 
      tcatacttt tgtgttata atattctta taatttttt 
      tctttcgcc aaaaatatg tttgtacga ttttagaaa 
      caaaaatta caagatttc ctcgaaatg gagaaagcc 
      aacgaattg ttacaacgc caagagttt gagttagca 
      aatgcacaa aaaattcat aaagaaaag cttttagcc 
      gatattgcg gaacaacag aataagctt gatattgag 
      ttgttgaag cttcaacaa gagaaggaa attgaacgg 
      tttcatttg attgagaaa gtgctcgaa gctgaacag 
      aattcagac cgcgccatc caacagctg ctcgaattg 
      aatagtgaa cctcttgcg cagcttttg cagcaagaa 
      aaggaagaa gaggagcga attttagct gcagctaat 
      aaatacaac agcgatttg cgtaaaact gatattttg 
      agcgatatg caagtttta ctagaacag gaattgact 
      aaatttcaa cagtttcac gagaatcgg atggaagtt 
      tcaaggggg attttagaa caggaaatg gattatgat 
      tatcgcatc agtgaaatc ctccaaaat caggatttg 
      cacaaagct gagcttttg agcaaattg caggaagac 
      agtgagttg caaaaagtg gcagtgggg gcgcttcta 
      gaaaggggc gacgcccga tcatggggg ctgttgcag 
      caagtgaag ttagttgag tcacagttg gcagcactg 
      actcacatc gagatggat aaaaagaaa cttgaaatt 
      gaggaaaat ctcaatgat ttatcagag aaaagaatt 
      caattgtcg tgtttattg atggacttg ttggaccag 
      cagaaagca cgaagggat cagttgttg tccacttta 
      caaaatttg gaagaattt aacaactcg gacgatttt 
      tggctcaaa caataccaa agtttgctg gaaaagctt 
      ccggaaggt ttgtcagag gctcaaaaa aatatggat 
      ccgctcctg gcacaagca cttttactg aatggagtc 
      attaattgt ttgccgttt ttggccagt ttgatgcaa 
      tgtcaatgc aacgttgca aaaatcaca gaaagcgac 
      ctcaaagaa gtgggaatt gtgaataaa tcggaccgg 
      gtccacatt ttagacgca ttccaaatg taccgaaag 
      gagcaaata ccgaccgat acggccccc tcagctcca 
      gtgctgcca atcgaagag gcaagtgcg ccaccactc 
      gaaaatgtc gcaaatttg gggactgaa tgcgtcatt 
      tgtttggac tcaacttgt gaggtgatt ttcgtgcca 
      tgtggacac ttctgttgc tgttcccag tgtcctgtg 
      accttgaac gactgtcca atgtgcagg acttcaatc 
      gaacgcaaa attagaata atttcatag ttcgttcaa 
      ctgccgtca ctttttttg ctcagtgtt gctaaagtg 
      tgaattctg cacgatttg tggccttaa tctagtcag 
      ttgttactt tttctcatg a
    • >TC002403 RB
      cgtagttta aacttaaaa aaaaatcca ataattttt 
      taagaaaag cgtgttttc gtcaaaaaa tcaatttca 
      gaatagacc aacaaattc cgaacatgg ttttgtatt 
      ctgaaacac ccgatttgg ggcaacctt atttaggcc 
      ccgaaacta ttgaagaca ctcctgata ctagcactg 
      ataacaacg aggaccaaa ctgatgctt ttgaggcca 
      aaatatgga agctagaaa atattaaga aacaaaaat 
      tacaagatt tcctcgaaa tggagaaag ccaacgaat 
      tgttacaac gccaagagt ttgagttag caaatgcac 
      aaaaaattc ataaagaaa agcttttag ccgatattg 
      cggaacaac agaataagc ttgatattg agttgttga 
      agcttcaac aagagaagg aaattgaac ggtttcatt 
      tgattgaga aagtgctcg aagctgaac agaattcag 
      accgcgcca tccaacagc tgctcgaat tgaatagtg 
      aacctcttg cgcagcttt tgcagcaag aaaaggaag 
      aagaggagc gaattttag ctgcagcta ataaataca 
      acagcgatt tgcgtaaaa ctgatattt tgagcgata 
      tgcaagttt tactagaac aggaattga ctaaatttc 
      aacagtttc acgagaatc ggatggaag tttcaaggg 
      ggattttag aacaggaaa tggattatg attatcgca 
      tcagtgaaa tcctccaaa atcaggatt tgcacaaag 
      ctgagcttt tgagcaaat tgcaggaag acagtgagt 
      tgcaaaaag tggcagtgg gggcgcttc tagaaaggg 
      gcgacgccc gatcatggg ggctgttgc agcaagtga 
      agttagttg agtcacagt tggcagcac tgactcaca 
      tcgagatgg ataaaaaga aacttgaaa ttgaggaaa 
      atctcaatg atttatcag agaaaagaa ttcaattgt 
      cgtgtttat tgatggact tgttggacc agcagaaag 
      cacgaaggg atcagttgt tgtccactt tacaaaatt 
      tggaagaat ttaacaact cggacgatt tttggctca 
      aacaatacc aaagtttgc tggaaaagc ttccggaag 
      gtttgtcag aggctcaaa aaaatatgg atccgctcc 
      tggcacaag cacttttac tgaatggag tcattaatt 
      gtttgccgt ttttggcca gtttgatgc aatgtcaat 
      gcaacgttg caaaaatca cagaaagcg acctcaaag 
      aagtgggaa ttgtgaata aatcggacc gggtccaca 
      ttttagacg cattccaaa tgtaccgaa aggagcaaa 
      taccgaccg atacggccc cctcagctc cagtgctgc 
      caatcgaag aggcaagtg cgccaccac tcgaaaatg 
      tcgcaaatt tggggactg aatgcgtca tttgtttgg 
      actcaactt gtgaggtga ttttcgtgc catgtggac 
      acttctgtt gctgttccc agtgtcctg tgaccttga 
      acgactgtc caatgtgca ggacttcaa tcgaacgca 
      aaattagaa taatttcat agttcgttc aactgccgt 
      cactttttt tgctcagtg ttgctaaag tgtgaattc 
      tgcacgatt tgtggcctt aatctagtc agttgttac 
      tttttctca tga