Status
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Gene information
General information
TC002392 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 31347250..31356332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002392
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late number decreased - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 85.71% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupation delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection



    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002392 RA
      MFWKYNNNS SSELDALLA KQDVTLSEV MDAEDIINQ 
      CKAQSKGLI DFLLKPEMM EELVSLVTR EPSNELDER 
      VRFKYPNIA CELLTSDVP SINERLASD EVLLDKLYS 
      FLECEPPLN PLLASYFSR IMGALIARK TEQVLDFLK 
      TKDTFISLL LKHLGTSAI MDLMLKLMT QVESLEMRQ 
      NILNWLDSQ RIMQSLVCL LNPKIDKER HYNVAQLLC 
      DFIKAARDT QRNSSERVD PDPLLNTLE SAETVSLLL 
      DNIFQEEKT ESAIVGGIQ VLLALLDIS QLSIPKINS 
      QISYNSNIN DEAIDIEHK EKVIKSTTE AILGHIKDF 
      HELLINPPI KSSINTSVG ILETPLGNT RLQVTKLFA 
      AIIASNNTR LAEEIVSMD TFSILLDLF FKYPWNNFL 
      HTQVENCLI SALKTHTTD ESNENSNAL STHLLVKCN 
      VIERILKAW KDNDEQQKA NGVRQGYMG HLISIINKV 
      VELCSNTSL GKYLIDNLP EVAKNLDEF KETTLKETN 
      SVQDTLLGG VYPHSTHED SNDYPGVTY NQNEIYCSY 
      QTHNLTPHC IDGYSGFND DEFNDGDDT LQSIDHRTD 
      MNFSLTDGD LAQQRELFK QMCAQNINT LDDADDQIF 
      EDRDHTFQT VIEKEDQND TVYSSDSDD DNGPAGVPM 
      DVDPWLSPK GGTGAPLEA ADPWGGAPQ STTTTTTTT 
      TSDQMGDWA DFSSVNFEA NFEAVFEKT PTRKSDDDD 
      LKVENIEEF EAKKTDDVI SIETVAQTA IKDDQNVEK 
      KEEKQAGDL LLGERKDTS QSVIEPSNA SVHVEGVKE 
      ALPPASETK DV
    CDS sequence
    • >TC002392 RA
      atgttttgg aaatacaac aacaattcg tcgtcggag 
      ctcgacgcc ctgctcgcc aaacaggac gtcacgttg 
      tcggaggtg atggacgct gaagacatc atcaaccag 
      tgcaaagcg caaagcaag ggcttgatc gactttctt 
      ttgaagccc gaaatgatg gaagagctt gtaagtctt 
      gtaacacgt gagccttcc aacgaattg gacgaaaga 
      gttaggttc aagtaccca aacattgcg tgtgaatta 
      cttacatct gatgttcct tcaattaac gagaggctg 
      gctagtgac gaagtactg cttgataag ctgtacagt 
      tttttagag tgtgagccg cccttaaac ccactctta 
      gcttcctac tttagtaga attatgggt gcacttatt 
      gccagaaag actgagcag gttctagat tttttaaaa 
      actaaggac acctttata tcattactg ctcaaacat 
      ttaggcact tccgctatc atggatctc atgctcaag 
      cttatgaca caggttgaa agcctcgaa atgagacaa 
      aatattctc aattggctt gatagtcag agaataatg 
      cagtcgttg gtttgtctt ttaaatccg aaaattgac 
      aaagaaagg cactataat gttgctcaa ttattgtgt 
      gattttatc aaagcagct cgtgatact caaagaaat 
      tcatcggaa agagttgat ccggatcca ctacttaat 
      acactagaa tcagctgaa acagtatca ttattgtta 
      gataatatt tttcaagag gaaaagacc gaaagtgct 
      attgttggc ggcattcaa gtactttta gcactttta 
      gatatttcc caattgagc attcctaaa ataaactcc 
      caaattagc tacaattcc aacataaac gacgaagca 
      attgacata gaacacaaa gagaaagtc attaaatcg 
      acaacggag gcaatcctt ggccacata aaagacttc 
      catgaatta ctaataaac ccgcccatt aaatcatca 
      ataaataca agcgtcggt attttggaa acacctctt 
      ggcaataca cgtcttcaa gtgactaaa ttatttgcc 
      gcaataata gcctcaaat aacacacgt ttggcggaa 
      gaaatcgtc tcaatggac acattttcc atactttta 
      gacttgttt tttaaatat ccctggaac aatttcctc 
      catactcaa gtggaaaat tgccttata tccgctttg 
      aagacacac accacggat gaatccaat gaaaattcg 
      aatgcctta agtacacac ttgttagtc aaatgtaat 
      gtgattgag cgaattttg aaagcctgg aaagacaac 
      gacgagcaa cagaaagcg aacggagta cgacagggt 
      tatatgggc catttaata agcataatt aacaaagta 
      gttgaattg tgttcaaat acttcacta gggaagtac 
      ctaatcgat aatttacca gaagtcgcc aaaaattta 
      gacgaattt aaggaaaca acactaaag gaaaccaat 
      tccgttcaa gacacttta ttgggtggg gtatatccg 
      cacagcacc cacgaggat agcaacgat tatcctggt 
      gtaacgtat aatcaaaac gagatttat tgcagttat 
      cagacccat aatctcaca ccgcactgc atcgatggt 
      tatagcggt ttcaacgat gatgaattc aacgatggt 
      gacgatact ttgcagtct atcgaccat cgaaccgat 
      atgaatttc agccttacc gacggtgat ttggcccaa 
      caacgtgaa ctattcaaa cagatgtgt gcccaaaat 
      attaataca cttgatgat gccgatgat caaattttt 
      gaagatcgt gatcataca tttcaaacg gtgattgaa 
      aaagaggat cagaatgat acggtctat agtagtgat 
      agtgatgat gataatgga ccggcgggg gttcccatg 
      gatgttgat ccttggctt tcaccaaaa gggggcaca 
      ggagccccc ttggaggcg gcagacccc tggggcgga 
      gccccacaa tccaccaca accactacc accaccacg 
      accagtgat caaatgggt gattgggcc gattttagt 
      tcggttaat ttcgaggcg aattttgag gccgttttt 
      gagaaaact ccaacacgt aaaagtgat gatgatgat 
      ttaaaagtg gaaaatatc gaagaattt gaagcgaaa 
      aaaacagac gatgtcatc agtattgaa acagttgct 
      caaacggcg attaaagac gaccaaaat gtcgagaaa 
      aaagaggag aaacaagcg ggtgattta ttattaggc 
      gaaagaaaa gacacgtca cagtcggtt attgaaccg 
      tccaatgcg tcagttcac gttgaggga gtcaaggaa 
      gctttgcct cctgctagt gaaacaaaa gacgtgtga
    mRNA sequence
    • >TC002392 RA
      tgcattgtg ggattagat ggtggtcat ttttgtcat 
      ttatattat tgtctaaaa gtgttgcaa ccaaccgaa 
      taaattgcg tgttttacc tgaagctcg tcttaatta 
      ctatgaaat ttgtgatat gctgtttat tatcgctag 
      ccgtatctg tgtaaagtg tgcatttaa ccaagcgtt 
      tgataacaa aggtcattt cagatgaac ctaacccaa 
      cgtctctag agtcacgaa aaagaaatt accatgttt 
      tggaaatac aacaacaat tcgtcgtcg gagctcgac 
      gccctgctc gccaaacag gacgtcacg ttgtcggag 
      gtgatggac gctgaagac atcatcaac cagtgcaaa 
      gcgcaaagc aagggcttg atcgacttt cttttgaag 
      cccgaaatg atggaagag cttgtaagt cttgtaaca 
      cgtgagcct tccaacgaa ttggacgaa agagttagg 
      ttcaagtac ccaaacatt gcgtgtgaa ttacttaca 
      tctgatgtt ccttcaatt aacgagagg ctggctagt 
      gacgaagta ctgcttgat aagctgtac agtttttta 
      gagtgtgag ccgccctta aacccactc ttagcttcc 
      tactttagt agaattatg ggtgcactt attgccaga 
      aagactgag caggttcta gatttttta aaaactaag 
      gacaccttt atatcatta ctgctcaaa catttaggc 
      acttccgct atcatggat ctcatgctc aagcttatg 
      acacaggtt gaaagcctc gaaatgaga caaaatatt 
      ctcaattgg cttgatagt cagagaata atgcagtcg 
      ttggtttgt cttttaaat ccgaaaatt gacaaagaa 
      aggcactat aatgttgct caattattg tgtgatttt 
      atcaaagca gctcgtgat actcaaaga aattcatcg 
      gaaagagtt gatccggat ccactactt aatacacta 
      gaatcagct gaaacagta tcattattg ttagataat 
      atttttcaa gaggaaaag accgaaagt gctattgtt 
      ggcggcatt caagtactt ttagcactt ttagatatt 
      tcccaattg agcattcct aaaataaac tcccaaatt 
      agctacaat tccaacata aacgacgaa gcaattgac 
      atagaacac aaagagaaa gtcattaaa tcgacaacg 
      gaggcaatc cttggccac ataaaagac ttccatgaa 
      ttactaata aacccgccc attaaatca tcaataaat 
      acaagcgtc ggtattttg gaaacacct cttggcaat 
      acacgtctt caagtgact aaattattt gccgcaata 
      atagcctca aataacaca cgtttggcg gaagaaatc 
      gtctcaatg gacacattt tccatactt ttagacttg 
      ttttttaaa tatccctgg aacaatttc ctccatact 
      caagtggaa aattgcctt atatccgct ttgaagaca 
      cacaccacg gatgaatcc aatgaaaat tcgaatgcc 
      ttaagtaca cacttgtta gtcaaatgt aatgtgatt 
      gagcgaatt ttgaaagcc tggaaagac aacgacgag 
      caacagaaa gcgaacgga gtacgacag ggttatatg 
      ggccattta ataagcata attaacaaa gtagttgaa 
      ttgtgttca aatacttca ctagggaag tacctaatc 
      gataattta ccagaagtc gccaaaaat ttagacgaa 
      tttaaggaa acaacacta aaggaaacc aattccgtt 
      caagacact ttattgggt ggggtatat ccgcacagc 
      acccacgag gatagcaac gattatcct ggtgtaacg 
      tataatcaa aacgagatt tattgcagt tatcagacc 
      cataatctc acaccgcac tgcatcgat ggttatagc 
      ggtttcaac gatgatgaa ttcaacgat ggtgacgat 
      actttgcag tctatcgac catcgaacc gatatgaat 
      ttcagcctt accgacggt gatttggcc caacaacgt 
      gaactattc aaacagatg tgtgcccaa aatattaat 
      acacttgat gatgccgat gatcaaatt tttgaagat 
      cgtgatcat acatttcaa acggtgatt gaaaaagag 
      gatcagaat gatacggtc tatagtagt gatagtgat 
      gatgataat ggaccggcg ggggttccc atggatgtt 
      gatccttgg ctttcacca aaagggggc acaggagcc 
      cccttggag gcggcagac ccctggggc ggagcccca 
      caatccacc acaaccact accaccacc acgaccagt 
      gatcaaatg ggtgattgg gccgatttt agttcggtt 
      aatttcgag gcgaatttt gaggccgtt tttgagaaa 
      actccaaca cgtaaaagt gatgatgat gatttaaaa 
      gtggaaaat atcgaagaa tttgaagcg aaaaaaaca 
      gacgatgtc atcagtatt gaaacagtt gctcaaacg 
      gcgattaaa gacgaccaa aatgtcgag aaaaaagag 
      gagaaacaa gcgggtgat ttattatta ggcgaaaga 
      aaagacacg tcacagtcg gttattgaa ccgtccaat 
      gcgtcagtt cacgttgag ggagtcaag gaagctttg 
      cctcctgct agtgaaaca aaagacgtg tgaataacg 
      cccccctcc cctacatat caaacggga acttaaact 
      gttagaatg aatgccata aaatgagat agtgttttg 
      taatcgaca cattttaat cattacagt aaaacacaa 
      aaaaactat cttttcata attttttaa catgattat 
      tcatctagc aatatttgt tgtaaaaaa atggttatt 
      taatttatt attggtatt tttttactt ttgatttga 
      tttggagta gaatcgttg gctttgtga agagatgcg 
      tttgttggg aaaaagtta ttgattcag ggttaaata 
      gtattaaag tgtgtttgt atttaaaat tgcttatcg 
      cttagtatt aatgtgatc gatctcttt gcaaagcat 
      acaatacag ggtgtctca gcgagttgg taaagtcta 
      ttattcacc tttaaatgt tacagaagt atttttttc 
      taagtcgta atgtggtgg aagtcctac ttttgtaga 
      agtcttaaa ttaaataaa ataaaagta taagtcaaa 
      attattaat cttaaaagc tacttcaaa caatgcaaa 
      aaaaatata agtttgtat tatagctca tttcagaaa 
      cagcctgta tatttgaaa atattttta ggtgaggta 
      tctacggct tagaaaaat aaaattatt acatttcca 
      atatttaga agagagtaa tgggacact ctgtattac 
      cgatatgga agattgaga tagattttg tgcattttt 
      tcggttgat tttagcaca aagtctgtt cagtattgt 
      gatataaac caatccgat gcaacttag aattgtagt 
      tactttatt gtgcctgtt aatgcaatc agaacttaa 
      accaaaaaa tgtaaaagt gtattcatc gaaacatat 
      gtaaatata ttattacta cattttcgt ttggacaaa 
      atgtacaaa tactagttt tcataatat gatgtgaca 
      tcaaccaat gttttacgt tttcatctc cacttctgt 
      gattttaaa tcgtggcta aattactac atagcacac 
      ttgtactat ttttattta gcggattcg accctcatg 
      aacagtgcc taggcatat tgtatattt tttaacatc 
      ctcgtactg ttattttgt tttcattgt cctattggt 
      tctaataaa atttattga atttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY