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Gene information
General information
TC002390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 31360554..31362212
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC002390
    iBeetle screen iB_00362

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupation delayed (still prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    remarks: animals lost, pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection

    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002390 RA
      MKVVKDNEN KWSGYGLRI FLNTLSHPF EYAKVLIQI 
GYEPIPPHS SKTLLGKPA LKLPNIFQY VKHIKSVDG 
LSGCYRGLG PKVCGSLIN LVATQKMME YLDLNQQDE 
EEDTDVEEN RKQKFLKCL KGDIITHTT AIIISQPFH 
VITIRIMAQ FVGKEAKYV GVFGSIGEI YRQNGILGF 
FSGLIPRLI GDILSVLLA SSLTYVVNK YLIEEKEIQ 
NYTSAAVCM VSSSIMYPF QVVSNCMAV SNSGLLAGS 
PPYMLHYNT WIDCWNDLS SRNQLKRGS SLLVRYYKG 
PSLITSKGN LLKGN
    CDS sequence
    • >TC002390 RA
      atgaaagtc gtcaaagac aacgaaaac aaatggtca 
ggatacgga cttcgcatt ttcctcaac accttatcg 
cacccattc gaatacgcc aaagtgctc atacagatc 
ggatacgaa cccataccg cctcactcg tccaaaacc 
ctgctcggt aaaccagcc ctcaaactg ccaaacatc 
ttccaatac gtcaaacac atcaagtcg gtcgatggt 
ttgtcgggt tgttatcgc ggcttgggc cccaaagta 
tgcggcagt ttgataaat cttgtcgct acacaaaaa 
atgatggaa tatttggac ttgaaccag caggacgaa 
gaagaggat acagatgtt gaggaaaat cgaaaacag 
aaatttttg aagtgtctc aagggcgat atcattaca 
catacaaca gccataatc atcagtcag ccgtttcac 
gtcatcact atacgaatt atggcacaa tttgttgga 
aaagaagcc aaatatgtg ggtgtgttt ggttccatt 
ggtgaaata tacagacag aatggaatt ttgggtttt 
ttcagcgga ctcataccc agactgatt ggggatatt 
ttgtccgtt ttgttggca agcagtctg acttatgtt 
gtcaataag tatctaata gaagagaag gaaatacaa 
aattatact tcagccgct gtgtgtatg gtgtcctca 
tcaataatg taccccttc caagtggtg tcaaattgc 
atggctgtc tcgaattcg ggtttgttg gctggatcg 
cccccttat atgttgcac tacaacact tggattgat 
tgttggaat gatttgtcg agtcgtaat caattgaaa 
agagggtct agtcttttg gttcgttat tacaagggt 
ccttcgttg ataacaagt aaaggcaat ctactcaag 
ggcaactaa
    mRNA sequence
    • >TC002390 RA
      ccgtgaaca tttgcaatt acatttatt taaaaaaac 
acaaaaata atcctccca aaacccgcc ttttcttca 
ttttttatc agtttatca cgcacaaaa agataagcg 
atcccagtt ggcacaatt gacagttgt gcccacttt 
tgacagaaa tcaaatcgt gtccaagtc tcaccaatt 
tcccaaaat ttccaaaaa caaacccac gatgaaagt 
cgtcaaaga caacgaaaa caaatggtc aggatacgg 
acttcgcat tttcctcaa caccttatc gcacccatt 
cgaatacgc caaagtgct catacagat cggatacga 
acccatacc gcctcactc gtccaaaac cctgctcgg 
taaaccagc cctcaaact gccaaacat cttccaata 
cgtcaaaca catcaagtc ggtcgatgg tttgtcggg 
ttgttatcg cggcttggg ccccaaagt atgcggcag 
tttgataaa tcttgtcgc tacacaaaa aatgatgga 
atatttgga cttgaacca gcaggacga agaagagga 
tacagatgt tgaggaaaa tcgaaaaca gaaattttt 
gaagtgtct caagggcga tatcattac acatacaac 
agccataat catcagtca gccgtttca cgtcatcac 
tatacgaat tatggcaca atttgttgg aaaagaagc 
caaatatgt gggtgtgtt tggttccat tggtgaaat 
atacagaca gaatggaat tttgggttt tttcagcgg 
actcatacc cagactgat tggggatat tttgtccgt 
tttgttggc aagcagtct gacttatgt tgtcaataa 
gtatctaat agaagagaa ggaaataca aaattatac 
ttcagccgc tgtgtgtat ggtgtcctc atcaataat 
gtacccctt ccaagtggt gtcaaattg catggctgt 
ctcgaattc gggtttgtt ggctggatc gccccctta 
tatgttgca ctacaacac ttggattga ttgttggaa 
tgatttgtc gagtcgtaa tcaattgaa aagagggtc 
tagtctttt ggttcgtta ttacaaggg tccttcgtt 
gataacaag taaaggcaa tctactcaa gggcaacta 
aatttcatg ttattcagt agttggatt ttttttcat 
tacaaattg acgcaattg tatgtaaat atttagagt 
attgtgtta ttttacaat aaaattctt gttcagat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY