Status
Please wait ...
Gene information
General information
TC002389 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 31363360..31368333
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002389
    iBeetle screen iB_00361

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002389 RA
      MSAKVLQYD THELETFLP HSQPTYINP VPTQPSPQV 
      DVKVTVSEN EKDLGQVKE DFDPFKARH LDQPVSSGA 
      TLTHLLKSS LGTGILSMP AAFKASGLW LGVITTMLV 
      SLICTHTAY ALVTSAHAL YRKAGKTSM SYAEVAEES 
      CLRGPPWAK KYAFLLKQL VLWAIFVTY YATGSCYAV 
      IVAENFNYV AFNYLGNFD KRITIAMLF LPFLLIAYV 
      PNLKYLAPV SMVANFCMA IGLGITCYY LLNDIPSIS 
      DRPAVTNLA TLPVCISIV IFAIEAIGV VMPLENNMK 
      SPQKFVGLF GVLNQGMTY VTILYIILG FLGYLKYGD 
      LTADSITLN LPREEYAAQ AVNLLIGLA VFFTYGLVF 
      YVCLDIFWT EIKHRFTTK TALANYALR TILVMINII 
      IAILVPAIV PFVGLIGAF CFSILGLVC PVIIEIFTF 
      WDQGFGKFY WKLFKHLVV VCMALLAVV FGSKAAISD 
      IAKTFH
    CDS sequence
    • >TC002389 RA
      atgtcggcc aaagtccta caatatgac acacacgag 
      ttggaaact ttcctccct cacagccaa ccaacttac 
      atcaatcca gtacccacc caacctagt cctcaagtt 
      gatgttaag gtgacagtg agtgaaaat gagaaagat 
      ctgggccaa gttaaggaa gattttgac ccgttcaaa 
      gcccgacat ttggaccaa cctgtctcg agcggcgcc 
      accctcacc cacctcctc aaatcctcc ctcggcacc 
      ggcatcctc tcaatgcct gcagcattc aaagcttcc 
      ggcctctgg cttggtgtc atcaccacc atgttggta 
      tccctaatt tgcacacac actgcctat gctctagtc 
      acctcggct cacgccttg taccgcaaa gcgggcaaa 
      acgtcgatg agctatgcg gaagttgcc gaagaatcc 
      tgtcttcgg ggacccccc tgggcgaaa aaatacgcc 
      ttcttgctc aaacaattg gtcttatgg gcgattttc 
      gtaacttat tacgccaca ggaagttgc tatgctgtt 
      atagttgcc gagaatttc aattacgtg gccttcaac 
      tatttaggg aattttgat aaaagaatc actatagct 
      atgttgttt ttgcccttt ttgctcatt gcctacgtg 
      ccaaatttg aagtatttg gcaccagta tccatggtt 
      gccaatttt tgtatggct ataggatta gggatcacg 
      tgttattat ctcctgaat gacattccg tccatttct 
      gacagaccg gcagtgaca aatctggca acgttgcct 
      gtttgcatc agtatagtg atttttgca attgaagct 
      atcggagtt gtaatgcca cttgaaaac aacatgaaa 
      tcgccacaa aaattcgtc ggacttttt ggagtactt 
      aatcaaggg atgacttat gtgaccatc ctgtatata 
      attttaggt ttcttaggg tatctcaaa tatggtgat 
      cttacggct gattcaatc acgttgaat cttccacga 
      gaggaatat gctgcccaa gctgtgaat ctccttatc 
      ggccttgca gtattcttc acttacggt ctagtgttt 
      tacgtgtgt ttggacatt ttctggacc gaaatcaag 
      caccgtttc accaccaaa acagcactt gctaattac 
      gccctaaga actattctt gtgatgata aatatcata 
      attgcgatt ctagtgcct gccattgta ccgtttgtc 
      ggtttaatc ggagcgttt tgtttctcg attttggga 
      cttgtatgt cctgtgatt atcgaaata tttacgttt 
      tgggatcag ggatttggg aagttttat tggaaattg 
      tttaaacat ttggtcgtt gtttgtatg gcgttgctt 
      gctgttgtt tttgggtcg aaagcggcc atctctgat 
      attgcaaaa acatttcac tga
    mRNA sequence
    • >TC002389 RA
      tgctgatca tgaagaaga gttgtgagt agaagcagt 
      cacactttt tccgtaaaa tgtcggcca aagtcctac 
      aatatgaca cacacgagt tggaaactt tcctccctc 
      acagccaac caacttaca tcaatccag tacccaccc 
      aacctagtc ctcaagttg atgttaagg tgacagtga 
      gtgaaaatg agaaagatc tgggccaag ttaaggaag 
      attttgacc cgttcaaag cccgacatt tggaccaac 
      ctgtctcga gcggcgcca ccctcaccc acctcctca 
      aatcctccc tcggcaccg gcatcctct caatgcctg 
      cagcattca aagcttccg gcctctggc ttggtgtca 
      tcaccacca tgttggtat ccctaattt gcacacaca 
      ctgcctatg ctctagtca cctcggctc acgccttgt 
      accgcaaag cgggcaaaa cgtcgatga gctatgcgg 
      aagttgccg aagaatcct gtcttcggg gacccccct 
      gggcgaaaa aatacgcct tcttgctca aacaattgg 
      tcttatggg cgattttcg taacttatt acgccacag 
      gaagttgct atgctgtta tagttgccg agaatttca 
      attacgtgg ccttcaact atttaggga attttgata 
      aaagaatca ctatagcta tgttgtttt tgccctttt 
      tgctcattg cctacgtgc caaatttga agtatttgg 
      caccagtat ccatggttg ccaattttt gtatggcta 
      taggattag ggatcacgt gttattatc tcctgaatg 
      acattccgt ccatttctg acagaccgg cagtgacaa 
      atctggcaa cgttgcctg tttgcatca gtatagtga 
      tttttgcaa ttgaagcta tcggagttg taatgccac 
      ttgaaaaca acatgaaat cgccacaaa aattcgtcg 
      gactttttg gagtactta atcaaggga tgacttatg 
      tgaccatcc tgtatataa ttttaggtt tcttagggt 
      atctcaaat atggtgatc ttacggctg attcaatca 
      cgttgaatc ttccacgag aggaatatg ctgcccaag 
      ctgtgaatc tccttatcg gccttgcag tattcttca 
      cttacggtc tagtgtttt acgtgtgtt tggacattt 
      tctggaccg aaatcaagc accgtttca ccaccaaaa 
      cagcacttg ctaattacg ccctaagaa ctattcttg 
      tgatgataa atatcataa ttgcgattc tagtgcctg 
      ccattgtac cgtttgtcg gtttaatcg gagcgtttt 
      gtttctcga ttttgggac ttgtatgtc ctgtgatta 
      tcgaaatat ttacgtttt gggatcagg gatttggga 
      agttttatt ggaaattgt ttaaacatt tggtcgttg 
      tttgtatgg cgttgcttg ctgttgttt ttgggtcga 
      aagcggcca tctctgata ttgcaaaaa catttcact 
      gaagctttg aatgctaat ataaagctc taatatttc 
      gaatcttcg agaaaaaaa aggttataa cgtaaaact 
      tgactattt atttgtgtt ctttgtttg taataaaat 
      taaaattac ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY