Status
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Gene information
General information
TC031758
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002372,TC002373
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 222766..232610
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031758
    iBeetle screen iB_00360

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031758 RA
      MAKEMMIIL VYDSQMLQK EEDDPASAI LYFHPTWVS 
      DQQKTALCG QLMGTVHCV KSIFSAPKI VSLQSGKFF 
      IKEYGRYLM AVGTDRNIA DWLLEHRAN TMSSLISFF 
      HQDIEIMSK LYDNSAKLS AKLYQLFET YLKYMFLGG 
      NIFSYTPSL KLPKSASNV FLEAIQILQ CCQELNYVM 
      GGTLLYHNK VVATQLSSD ITKRIVLTD PYRIKCPAE 
      TPSVNFELP LGVQLLQVY ISSKEYYKL HEEATRSRS 
      IFQYLSSKS IKKGKPVAS KEPVISAMK RDQSIIFTA 
      VPEEDSEPQ ISKVEKPIS SQNRPKFLN LKHKTTDEK 
      KPVAPSTPF HGQTSVCST PMTDLSKVL HSKPLSICI 
      NETIPCEKT PEKTSIFAK NGTDLNSVI ARVPYLTVT 
      SNLHDCKKF SSVFDVREK LKSLDRGIT MKYYNSEFR 
      EKYKISHDV TPDESRVFK TITDPNYPI FRSDGTVVS 
      HPFYQDYLT SQMELITSE IKEEKPDLS HHSFDDFDS 
      NLGDFVQSV KKIPDKVVE SIEFSALPR PSKEHRKSL 
      TLPLKSLSV DGGGEEVSP MRRHSSSVL LTPLMSKLS 
      SFESSGFCS RDTTPIFTP TQPNFPFAF KRPKKLLPE 
      TDSLRKCVL FVCGQQDMV VTLLLQDEA CASLELLTK 
      LWEICTENL GKLEKQLHH CLETYPGGG APGDTEPYS 
      YLYLDSDWD TIHRGGPWG SAELGALTY FHRDFQESS 
      SLIEILMRC CDGVIYGYQ CGKSEVFYH QAANPNAGL 
      PTPADPMSN VPLKARRRL ERDHSIILL
    • >TC031758 RB
      MAKEMMIIL VYDSQMLQK EEDDPASAI LYFHPTWVS 
      DQQKTALCG QLMGTVHCV KSIFSAPKI VSLQSGKFF 
      IKEYGRYLM AVGTDRNIA DWLLEHRAN TMSSLISFF 
      HQDIEIMSK LYDNSAKLS AKLYQLFET YLKYMFLGG 
      NIFSYTPSL KLPKSASNV FLEAIQILQ CCQELNYVM 
      GGTLLYHNK VVATQLSSD ITKRIVLTD PYRIKCPAE 
      TPSVNFELP LGVQLLQVY ISSKEYYKL HEEATRSRS 
      IFQYLSSKS IKKGKPVAS KEPVISAMK RDQSIIFTA 
      VPEEDSEPQ ISKVEKPIS SQNRPKFLN LKHKTTDEK 
      KPVAPSTPF HGQTSVCST PMTDLSKVL HSKPLSICI 
      NETIPCEKT PEKTSIFAK NGTDLNSVI ARVPYLTVT 
      SNLHDCKKF SSVFDVREK LKSLDRGIT MKYYNSEFR 
      EKYKISHDV TPDESRVFK TITDPNYPI FRSDGTVVS 
      HPFYQDYLT SQMELITSE IKEEKPDLS HHSFDDFDS 
      NLGDFVQSV KKIPDKVVE SIEFSALPR PSKEHRKSL 
      TLPLKSLSV DGGGEEVSP MRRHSSSVL LTPLMSKLS 
      SFESSGFCS RDTTPIFTP TQPNFPFAF KRPKKLLPE 
      TDSLRKCVL FVCGQQDMV VTLLLQDEA CASLELLTK 
      LWEICTENL GKLEKQLHH CLETYPGGG APGDTEPYS 
      YLYLDSDWD TIHRGGPWG SAELGALTY FHRDFQESS 
      SLIEILMR
    CDS sequence
    • >TC031758 RA
      atggcaaag gaaatgatg ataatccta gtctatgac 
      tctcagatg ctccagaaa gaagaagac gaccctgcg 
      agcgcaatt ttgtatttc cacccaacg tgggtgtca 
      gaccagcaa aaaacggct ttgtgtggc cagctaatg 
      ggcaccgtt cattgcgtt aaaagtatt ttttccgcc 
      ccgaaaata gtctcgtta cagagcggc aaatttttt 
      attaaggag tatgggcgg tatctgatg gccgtaggc 
      accgatcgc aacattgcg gattggtta ctggaacat 
      agggccaac acaatgtct tcactgata agcttcttc 
      caccaggac attgaaatc atgtcgaag ttgtacgac 
      aacagcgcg aaactctcc gcaaaactg tatcaatta 
      ttcgagact tatctaaag tacatgttt ctgggcggc 
      aatattttt tcctatact ccgtcgctg aaactacca 
      aagagcgca agtaatgtg tttttagag gccatacaa 
      atactacag tgttgccaa gaactcaat tatgtcatg 
      ggcggcacg ttgttgtac cataacaag gtcgtagca 
      actcagctg agttcagac ataacgaaa cgaattgta 
      ttaacggat ccgtaccga attaaatgc ccggcagag 
      acacctagc gtcaatttt gagctacct ctaggcgtc 
      caactgctg caagtatac atttcgagt aaagaatat 
      tacaaattg cacgaggag gcgacaagg agtcgcagt 
      attttccag tacctgagc agcaaaagt attaaaaag 
      ggaaaacca gtggcgtcc aaggagccg gtgatatcc 
      gccatgaaa cgtgaccaa tcaatcatt ttcacagca 
      gtccccgaa gaagacagc gagccccaa atcagcaaa 
      gtcgagaaa ccaatttcg tcacaaaac cgccccaaa 
      ttcctaaac ttgaaacac aaaacaacc gacgagaaa 
      aaacctgtg gcacctagt acacccttc catggccag 
      actagcgtg tgttcgacc ccaatgacc gatttgagt 
      aaagtttta cactcaaaa ccgttgtcg atttgcata 
      aacgagaca attccgtgc gagaaaacg ccggaaaaa 
      acctcaatt ttcgcgaaa aatggcacg gatttgaac 
      agtgttata gcaagagtg ccgtatttg acggtaacg 
      agcaatttg cacgattgc aaaaaattt tcaagtgtg 
      tttgacgtc cgggaaaag ttgaaaagt ctcgataga 
      ggcatcacg atgaagtat tacaattcg gagtttcgc 
      gaaaagtac aaaatttcg catgatgtt acaccagac 
      gaaagccga gtgtttaaa actataact gatccaaac 
      tatcccatt tttcgctcc gatgggacg gtggtctcg 
      catccgttt taccaagac tacttgacc agtcaaatg 
      gagctaatc acgagcgaa atcaaggaa gaaaagccc 
      gatttgagt catcactcg tttgacgac tttgactca 
      aacttgggt gattttgtc caaagtgtt aagaaaatt 
      ccggacaaa gtggttgaa agtattgaa ttctcggcc 
      ctgcctcgg ccgagcaaa gagcaccgg aaatcgcta 
      acacttccg ttaaaatca ctaagtgtg gacggtgga 
      ggggaggag gtttcacca atgaggaga cactcctcg 
      agtgttctc ctgactcct ttgatgtcg aaattgagc 
      tcgttcgaa tctagtggt ttctgcagc cgggacaca 
      accccgatt tttacgcca actcaacca aattttcca 
      tttgcgttc aaaaggccg aaaaagttg ctaccggag 
      acggattcc ttgcggaaa tgtgttttg tttgtttgt 
      ggacagcag gatatggtc gtgacgctt ttgttgcag 
      gatgaggca tgtgcttcg ttggaactt ttgaccaag 
      ctgtgggag atttgtaca gaaaatttg gggaagttg 
      gagaaacag ttgcatcac tgtttggag acgtatccg 
      ggcggaggg gctccagga gataccgag ccttacagt 
      tatctgtat ttggactcg gattgggac actatacat 
      cgtgggggg ccctggggg agtgccgag ttgggggct 
      ctgacgtat tttcatcga gattttcag gagtcgagc 
      agtttgatc gagatttta atgagatgt tgtgacggc 
      gtaatttac ggctatcag tgtggcaag tctgaggtg 
      ttttaccac caggctgcc aaccccaat gcagggttg 
      ccaacaccg gcggacccc atgagcaac gttccactg 
      aaagcaagg cggcggctg gaacgcgac cactcaatt 
      attttattg taa
    • >TC031758 RB
      atggcaaag gaaatgatg ataatccta gtctatgac 
      tctcagatg ctccagaaa gaagaagac gaccctgcg 
      agcgcaatt ttgtatttc cacccaacg tgggtgtca 
      gaccagcaa aaaacggct ttgtgtggc cagctaatg 
      ggcaccgtt cattgcgtt aaaagtatt ttttccgcc 
      ccgaaaata gtctcgtta cagagcggc aaatttttt 
      attaaggag tatgggcgg tatctgatg gccgtaggc 
      accgatcgc aacattgcg gattggtta ctggaacat 
      agggccaac acaatgtct tcactgata agcttcttc 
      caccaggac attgaaatc atgtcgaag ttgtacgac 
      aacagcgcg aaactctcc gcaaaactg tatcaatta 
      ttcgagact tatctaaag tacatgttt ctgggcggc 
      aatattttt tcctatact ccgtcgctg aaactacca 
      aagagcgca agtaatgtg tttttagag gccatacaa 
      atactacag tgttgccaa gaactcaat tatgtcatg 
      ggcggcacg ttgttgtac cataacaag gtcgtagca 
      actcagctg agttcagac ataacgaaa cgaattgta 
      ttaacggat ccgtaccga attaaatgc ccggcagag 
      acacctagc gtcaatttt gagctacct ctaggcgtc 
      caactgctg caagtatac atttcgagt aaagaatat 
      tacaaattg cacgaggag gcgacaagg agtcgcagt 
      attttccag tacctgagc agcaaaagt attaaaaag 
      ggaaaacca gtggcgtcc aaggagccg gtgatatcc 
      gccatgaaa cgtgaccaa tcaatcatt ttcacagca 
      gtccccgaa gaagacagc gagccccaa atcagcaaa 
      gtcgagaaa ccaatttcg tcacaaaac cgccccaaa 
      ttcctaaac ttgaaacac aaaacaacc gacgagaaa 
      aaacctgtg gcacctagt acacccttc catggccag 
      actagcgtg tgttcgacc ccaatgacc gatttgagt 
      aaagtttta cactcaaaa ccgttgtcg atttgcata 
      aacgagaca attccgtgc gagaaaacg ccggaaaaa 
      acctcaatt ttcgcgaaa aatggcacg gatttgaac 
      agtgttata gcaagagtg ccgtatttg acggtaacg 
      agcaatttg cacgattgc aaaaaattt tcaagtgtg 
      tttgacgtc cgggaaaag ttgaaaagt ctcgataga 
      ggcatcacg atgaagtat tacaattcg gagtttcgc 
      gaaaagtac aaaatttcg catgatgtt acaccagac 
      gaaagccga gtgtttaaa actataact gatccaaac 
      tatcccatt tttcgctcc gatgggacg gtggtctcg 
      catccgttt taccaagac tacttgacc agtcaaatg 
      gagctaatc acgagcgaa atcaaggaa gaaaagccc 
      gatttgagt catcactcg tttgacgac tttgactca 
      aacttgggt gattttgtc caaagtgtt aagaaaatt 
      ccggacaaa gtggttgaa agtattgaa ttctcggcc 
      ctgcctcgg ccgagcaaa gagcaccgg aaatcgcta 
      acacttccg ttaaaatca ctaagtgtg gacggtgga 
      ggggaggag gtttcacca atgaggaga cactcctcg 
      agtgttctc ctgactcct ttgatgtcg aaattgagc 
      tcgttcgaa tctagtggt ttctgcagc cgggacaca 
      accccgatt tttacgcca actcaacca aattttcca 
      tttgcgttc aaaaggccg aaaaagttg ctaccggag 
      acggattcc ttgcggaaa tgtgttttg tttgtttgt 
      ggacagcag gatatggtc gtgacgctt ttgttgcag 
      gatgaggca tgtgcttcg ttggaactt ttgaccaag 
      ctgtgggag atttgtaca gaaaatttg gggaagttg 
      gagaaacag ttgcatcac tgtttggag acgtatccg 
      ggcggaggg gctccagga gataccgag ccttacagt 
      tatctgtat ttggactcg gattgggac actatacat 
      cgtgggggg ccctggggg agtgccgag ttgggggct 
      ctgacgtat tttcatcga gattttcag gagtcgagc 
      agtttgatc gagatttta atgaggtaa
    mRNA sequence
    • >TC031758 RA
      gtacaggta ttttggaca tttccagtg gcgtatcag 
      tgtttttat tgttttaat caatcacca gtttttcaa 
      aaacccgga caaaaacat gatttaacg cgtaggtaa 
      cgttacaag tcaattaag gaattatta cataacttg 
      tcaggtatt tgtttactt gcgaaaagt gaggttatg 
      tcacagttg tgtaaacat caataaaaa gtcttgtgt 
      ttatattga gtgaaaaat tcacaagaa aatggcaaa 
      ggaaatgat gataatcct agtctatga ctctcagat 
      gctccagaa agaagaaga cgaccctgc gagcgcaat 
      tttgtattt ccacccaac gtgggtgtc agaccagca 
      aaaaacggc tttgtgtgg ccagctaat gggcaccgt 
      tcattgcgt taaaagtat tttttccgc cccgaaaat 
      agtctcgtt acagagcgg caaattttt tattaagga 
      gtatgggcg gtatctgat ggccgtagg caccgatcg 
      caacattgc ggattggtt actggaaca tagggccaa 
      cacaatgtc ttcactgat aagcttctt ccaccagga 
      cattgaaat catgtcgaa gttgtacga caacagcgc 
      gaaactctc cgcaaaact gtatcaatt attcgagac 
      ttatctaaa gtacatgtt tctgggcgg caatatttt 
      ttcctatac tccgtcgct gaaactacc aaagagcgc 
      aagtaatgt gtttttaga ggccataca aatactaca 
      gtgttgcca agaactcaa ttatgtcat gggcggcac 
      gttgttgta ccataacaa ggtcgtagc aactcagct 
      gagttcaga cataacgaa acgaattgt attaacgga 
      tccgtaccg aattaaatg cccggcaga gacacctag 
      cgtcaattt tgagctacc tctaggcgt ccaactgct 
      gcaagtata catttcgag taaagaata ttacaaatt 
      gcacgagga ggcgacaag gagtcgcag tattttcca 
      gtacctgag cagcaaaag tattaaaaa gggaaaacc 
      agtggcgtc caaggagcc ggtgatatc cgccatgaa 
      acgtgacca atcaatcat tttcacagc agtccccga 
      agaagacag cgagcccca aatcagcaa agtcgagaa 
      accaatttc gtcacaaaa ccgccccaa attcctaaa 
      cttgaaaca caaaacaac cgacgagaa aaaacctgt 
      ggcacctag tacaccctt ccatggcca gactagcgt 
      gtgttcgac cccaatgac cgatttgag taaagtttt 
      acactcaaa accgttgtc gatttgcat aaacgagac 
      aattccgtg cgagaaaac gccggaaaa aacctcaat 
      tttcgcgaa aaatggcac ggatttgaa cagtgttat 
      agcaagagt gccgtattt gacggtaac gagcaattt 
      gcacgattg caaaaaatt ttcaagtgt gtttgacgt 
      ccgggaaaa gttgaaaag tctcgatag aggcatcac 
      gatgaagta ttacaattc ggagtttcg cgaaaagta 
      caaaatttc gcatgatgt tacaccaga cgaaagccg 
      agtgtttaa aactataac tgatccaaa ctatcccat 
      ttttcgctc cgatgggac ggtggtctc gcatccgtt 
      ttaccaaga ctacttgac cagtcaaat ggagctaat 
      cacgagcga aatcaagga agaaaagcc cgatttgag 
      tcatcactc gtttgacga ctttgactc aaacttggg 
      tgattttgt ccaaagtgt taagaaaat tccggacaa 
      agtggttga aagtattga attctcggc cctgcctcg 
      gccgagcaa agagcaccg gaaatcgct aacacttcc 
      gttaaaatc actaagtgt ggacggtgg aggggagga 
      ggtttcacc aatgaggag acactcctc gagtgttct 
      cctgactcc tttgatgtc gaaattgag ctcgttcga 
      atctagtgg tttctgcag ccgggacac aaccccgat 
      ttttacgcc aactcaacc aaattttcc atttgcgtt 
      caaaaggcc gaaaaagtt gctaccgga gacggattc 
      cttgcggaa atgtgtttt gtttgtttg tggacagca 
      ggatatggt cgtgacgct tttgttgca ggatgaggc 
      atgtgcttc gttggaact tttgaccaa gctgtggga 
      gatttgtac agaaaattt ggggaagtt ggagaaaca 
      gttgcatca ctgtttgga gacgtatcc gggcggagg 
      ggctccagg agataccga gccttacag ttatctgta 
      tttggactc ggattggga cactataca tcgtggggg 
      gccctgggg gagtgccga gttgggggc tctgacgta 
      ttttcatcg agattttca ggagtcgag cagtttgat 
      cgagatttt aatgagatg ttgtgacgg cgtaattta 
      cggctatca gtgtggcaa gtctgaggt gttttacca 
      ccaggctgc caaccccaa tgcagggtt gccaacacc 
      ggcggaccc catgagcaa cgttccact gaaagcaag 
      gcggcggct ggaacgcga ccactcaat tattttatt 
      gtaagacag tattgccaa cattccatt agtgtatcg 
      tgccaaaaa tgtgtatc
    • >TC031758 RB
      gtacaggta ttttggaca tttccagtg gcgtatcag 
      tgtttttat tgttttaat caatcacca gtttttcaa 
      aaacccgga caaaaacat gatttaacg cgtaggtaa 
      cgttacaag tcaattaag gaattatta cataacttg 
      tcaggtatt tgtttactt gcgaaaagt gaggttatg 
      tcacagttg tgtaaacat caataaaaa gtcttgtgt 
      ttatattga gtgaaaaat tcacaagaa aatggcaaa 
      ggaaatgat gataatcct agtctatga ctctcagat 
      gctccagaa agaagaaga cgaccctgc gagcgcaat 
      tttgtattt ccacccaac gtgggtgtc agaccagca 
      aaaaacggc tttgtgtgg ccagctaat gggcaccgt 
      tcattgcgt taaaagtat tttttccgc cccgaaaat 
      agtctcgtt acagagcgg caaattttt tattaagga 
      gtatgggcg gtatctgat ggccgtagg caccgatcg 
      caacattgc ggattggtt actggaaca tagggccaa 
      cacaatgtc ttcactgat aagcttctt ccaccagga 
      cattgaaat catgtcgaa gttgtacga caacagcgc 
      gaaactctc cgcaaaact gtatcaatt attcgagac 
      ttatctaaa gtacatgtt tctgggcgg caatatttt 
      ttcctatac tccgtcgct gaaactacc aaagagcgc 
      aagtaatgt gtttttaga ggccataca aatactaca 
      gtgttgcca agaactcaa ttatgtcat gggcggcac 
      gttgttgta ccataacaa ggtcgtagc aactcagct 
      gagttcaga cataacgaa acgaattgt attaacgga 
      tccgtaccg aattaaatg cccggcaga gacacctag 
      cgtcaattt tgagctacc tctaggcgt ccaactgct 
      gcaagtata catttcgag taaagaata ttacaaatt 
      gcacgagga ggcgacaag gagtcgcag tattttcca 
      gtacctgag cagcaaaag tattaaaaa gggaaaacc 
      agtggcgtc caaggagcc ggtgatatc cgccatgaa 
      acgtgacca atcaatcat tttcacagc agtccccga 
      agaagacag cgagcccca aatcagcaa agtcgagaa 
      accaatttc gtcacaaaa ccgccccaa attcctaaa 
      cttgaaaca caaaacaac cgacgagaa aaaacctgt 
      ggcacctag tacaccctt ccatggcca gactagcgt 
      gtgttcgac cccaatgac cgatttgag taaagtttt 
      acactcaaa accgttgtc gatttgcat aaacgagac 
      aattccgtg cgagaaaac gccggaaaa aacctcaat 
      tttcgcgaa aaatggcac ggatttgaa cagtgttat 
      agcaagagt gccgtattt gacggtaac gagcaattt 
      gcacgattg caaaaaatt ttcaagtgt gtttgacgt 
      ccgggaaaa gttgaaaag tctcgatag aggcatcac 
      gatgaagta ttacaattc ggagtttcg cgaaaagta 
      caaaatttc gcatgatgt tacaccaga cgaaagccg 
      agtgtttaa aactataac tgatccaaa ctatcccat 
      ttttcgctc cgatgggac ggtggtctc gcatccgtt 
      ttaccaaga ctacttgac cagtcaaat ggagctaat 
      cacgagcga aatcaagga agaaaagcc cgatttgag 
      tcatcactc gtttgacga ctttgactc aaacttggg 
      tgattttgt ccaaagtgt taagaaaat tccggacaa 
      agtggttga aagtattga attctcggc cctgcctcg 
      gccgagcaa agagcaccg gaaatcgct aacacttcc 
      gttaaaatc actaagtgt ggacggtgg aggggagga 
      ggtttcacc aatgaggag acactcctc gagtgttct 
      cctgactcc tttgatgtc gaaattgag ctcgttcga 
      atctagtgg tttctgcag ccgggacac aaccccgat 
      ttttacgcc aactcaacc aaattttcc atttgcgtt 
      caaaaggcc gaaaaagtt gctaccgga gacggattc 
      cttgcggaa atgtgtttt gtttgtttg tggacagca 
      ggatatggt cgtgacgct tttgttgca ggatgaggc 
      atgtgcttc gttggaact tttgaccaa gctgtggga 
      gatttgtac agaaaattt ggggaagtt ggagaaaca 
      gttgcatca ctgtttgga gacgtatcc gggcggagg 
      ggctccagg agataccga gccttacag ttatctgta 
      tttggactc ggattggga cactataca tcgtggggg 
      gccctgggg gagtgccga gttgggggc tctgacgta 
      ttttcatcg agattttca ggagtcgag cagtttgat 
      cgagatttt aatgaggta aaattatca aaatatttt 
      caagttatg aaaaaattt gactaattt gtgagacat 
      ttcttagcc aagaatcgg tgaaaatca agtttatta 
      aaaacaatc tagaaaatc cttttcgat aacagttaa 
      tccgtttga gttatctca aggcacact tttgaataa 
      ggaagtatt agaaatcaa ctcatttta atgaaatca 
      tcgtagtgt ttagcctga ataaggtga tttcaactt 
      ttcaaaaaa gattttttt tgagatttt tttgttttc 
      aagcaaata aattaaaat caagttttt ttaaactta 
      actggtggt taaatcgtc gttgaataa aacgtgttt 
      agtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY