| General information | |||
| TC031122
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452276.1: 356..21373 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031122 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00359 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC031122 RA
MSFLVSFSS RFFLSPLRR VPPIPNLLN ELPEVPLFR RRPSPSFRR AQRTRLVHS VAVSVEQPL RGAPCSDRS TRKRFCFLA KESAMPTGK PFERLPETV RPKHYVLEL VPDLKALVF DGTVAVQIE VVKPTNEIV LNAIDLEIK ETTLKGPNV LTPDSTNFS VEDETVTFN FAKPLTPGS YTLSMRFKG ELNDKMKGL YRSKYQNQK GEERYAAVT QFEATDARR CFPCWDEPA LKATFDITL TVPKDLVAL SNMPVKQSK PQGDLIRYD FATTPIMST YLVACVVGE YDYVEDKST DGVQVRVYT PRGKKEQGL FALEVATKV LPYYKDYFN IAYPLPKID LIAIADFSA GAMENWGLV TYRETCLLV DPQNTSAVQ KQWIALVVG HELAHQWFG NLVTMEWWT HLWLNEGYA SFVEYLCVD HLFPEYDIW TQFVNDTYI RALELDCLK NSHPIEVPV GHPSEIDEI FDDISYNKG ASVIRMLHN YIGDEDFRK GMNLYLTRH QYKNTFTED LWAALEEAS NKPVGAVMS TWTKQMGFP VIKVTSRPD NNKGVVLTL AQSKYTADG SKAPDDFLW MIPVSIITS KQKNKPIST VLKTKEAEV VIPDVGPND WIKVNPGTV GFYRTQYAP DLLAKFIPA IKDRSLPPL DRLGLLDDL FAMVQAGHT NTVEVLKLL EAFTDETDY TVWSSINNV LVKLNMLLS YTDCADDFK TYQKRILSK IYKRLGWNP KATERHLDT LLRGLVLGR LSWLDDDDT IAEAKKRFE GHVNSSQTL PADLRSACY KTVLRAGGE DVYNTLLKL YRSVDLHEE KDRISRALG AAKDPEILS RVLKFAISV R
- >TC031122 RA
atgtcattt ctcgtcagt ttttcatcc cgttttttc ctgtcgccc ttgcgtaga gtccccccg atcccgaat ttattgaat gaattgccg gaagtaccg ttattccgc cgccgtccc tccccctcc ttccggcgg gcgcagcgt acgcgttta gttcattcg gttgccgtg tcagtggag cagccgctt cgtggagca ccttgtagt gaccgtagt acgcgtaaa aggttctgt tttctcgcc aaggagtcc gcaatgccc actgggaag ccgttcgag aggctgccg gagacggtc agacccaaa cactacgtg ctggagctg gtgccggac ctgaaggcg ctcgtcttc gatggaacc gtcgccgtg caaattgag gttgtgaag ccaacaaac gagattgta cttaacgcc atcgactta gaaatcaaa gaaacgacc ttaaaaggc cccaatgtc ttaacaccg gactcgacc aacttctcg gtcgaagac gaaaccgta acatttaat ttcgccaaa ccgctaaca cccggctct tacacgctc agtatgcgc ttcaaaggc gaactcaac gacaaaatg aaaggtctc taccgaagc aaataccag aaccaaaaa ggcgaagaa cgctacgct gccgtcaca cagttcgaa gcaaccgac gcccgtcgg tgcttcccc tgctgggac gaacccgcc ctcaaagcc accttcgac atcacccta accgtaccc aaagatcta gtcgctttg agcaacatg cctgtcaaa caatcgaag ccccaaggc gacctgatt cgctacgac ttcgcaaca actccaatc atgtccacc tatttggtc gcctgtgtg gtgggggag tacgattac gtggaggac aaatcaaca gatggggtg caagtcagg gtgtacaca ccacggggg aagaaggag caagggcta tttgcgctg gaagtggca acgaaagtg ctaccctat tataaggat tatttcaat attgcgtac cccctgcct aaaatcgat ctgattgcg atcgcagat ttcagcgcg ggggctatg gaaaactgg ggcttggtc acgtaccgt gagacctgc cttttggtg gacccccag aacacctca gctgtgcaa aaacagtgg attgcgctc gtagtgggc catgagttg gcccaccaa tggttcggc aacttggtc acgatggag tggtggacg catttatgg ttgaacgag ggctacgcc tcctttgtg gagtatttg tgcgtcgat catttgttc cccgagtac gatatttgg acgcagttt gttaatgac acgtatatt cgggctttg gagctcgat tgtttgaaa aactcgcac ccgatcgaa gttccggtc ggtcatccc tcggaaatc gacgaaatt ttcgacgat atcagctat aataagggg gcgtcggtt atccgaatg ctgcataat tatatcgga gatgaagat tttcgcaaa gggatgaat ttgtactta acgcgacat cagtataaa aacacgttt actgaggat ctttgggct gcgctggag gaggcgtcc aataaaccg gtgggtgct gtcatgtcg acttggacc aagcaaatg ggttttccg gtgattaag gttacgagc cgacctgat aataacaaa ggggtggtt ttgacgctt gctcaaagt aaatatacg gctgatggg tcgaaagca ccggatgat ttcttgtgg atgattccg gtgtcgatt atcacgtcg aaacagaaa aataagccc attagtact gtacttaag acgaaggaa gcggaggtt gtcattccg gatgttggt ccaaatgat tggattaag gtgaatccg gggactgtt ggcttctac cgcactcag tacgctccg gaccttctt gccaaattt attccggcg atcaaagac cggagtttg cctccactg gaccgactt ggtcttttg gatgatctg ttcgcaatg gtccaagcc ggacatacg aacaccgtc gaggtcttg aaactactt gaagctttc accgacgag acggactat acagtctgg tcctcaatc aacaatgtc ttggttaag ttaaacatg ttgttgagc tataccgac tgtgcggac gacttcaaa acgtaccag aaacgcatt ttgagcaag atttacaaa cggttgggg tggaacccc aaggctacg gaacgccat ctcgacacc ctcctccgg gggctggtt ttgggccgc ttgtcgtgg ctcgatgac gacgacacc atcgccgag gccaagaaa cgtttcgaa ggtcacgtc aattctagt caaacttta ccggcggat ttacgaagt gcctgctac aaaactgta cttagagcg gggggcgaa gatgtttac aacacgctt ttgaaattg tatagatcg gtggattta cacgaggag aaggatcgg atcagtcga gcgctgggt gcagcgaag gatcctgag atcttgtca agggtcttg aagttcgcc atatcggta aggtag
- >TC031122 RA
ggcagctcg accatgtga ccggccccg tttttctca attcccgaa gtgggctgg cgtcgaaaa taatgtcat ttctcgtca gtttttcat cccgttttt tcctgtcgc ccttgcgta gagtccccc cgatcccga atttattga atgaattgc cggaagtac cgttattcc gccgccgtc cctccccct ccttccggc gggcgcagc gtacgcgtt tagttcatt cggttgccg tgtcagtgg agcagccgc ttcgtggag caccttgta gtgaccgta gtacgcgta aaaggttct gttttctcg ccaaggagt ccgcaatgc ccactggga agccgttcg agaggctgc cggagacgg tcagaccca aacactacg tgctggagc tggtgccgg acctgaagg cgctcgtct tcgatggaa ccgtcgccg tgcaaattg aggttgtga agccaacaa acgagattg tacttaacg ccatcgact tagaaatca aagaaacga ccttaaaag gccccaatg tcttaacac cggactcga ccaacttct cggtcgaag acgaaaccg taacattta atttcgcca aaccgctaa cacccggct cttacacgc tcagtatgc gcttcaaag gcgaactca acgacaaaa tgaaaggtc tctaccgaa gcaaatacc agaaccaaa aaggcgaag aacgctacg ctgccgtca cacagttcg aagcaaccg acgcccgtc ggtgcttcc cctgctggg acgaacccg ccctcaaag ccaccttcg acatcaccc taaccgtac ccaaagatc tagtcgctt tgagcaaca tgcctgtca aacaatcga agccccaag gcgacctga ttcgctacg acttcgcaa caactccaa tcatgtcca cctatttgg tcgcctgtg tggtggggg agtacgatt acgtggagg acaaatcaa cagatgggg tgcaagtca gggtgtaca caccacggg ggaagaagg agcaagggc tatttgcgc tggaagtgg caacgaaag tgctaccct attataagg attatttca atattgcgt accccctgc ctaaaatcg atctgattg cgatcgcag atttcagcg cgggggcta tggaaaact ggggcttgg tcacgtacc gtgagacct gccttttgg tggaccccc agaacacct cagctgtgc aaaaacagt ggattgcgc tcgtagtgg gccatgagt tggcccacc aatggttcg gcaacttgg tcacgatgg agtggtgga cgcatttat ggttgaacg agggctacg cctcctttg tggagtatt tgtgcgtcg atcatttgt tccccgagt acgatattt ggacgcagt ttgttaatg acacgtata ttcgggctt tggagctcg attgtttga aaaactcgc acccgatcg aagttccgg tcggtcatc cctcggaaa tcgacgaaa ttttcgacg atatcagct ataataagg gggcgtcgg ttatccgaa tgctgcata attatatcg gagatgaag attttcgca aagggatga atttgtact taacgcgac atcagtata aaaacacgt ttactgagg atctttggg ctgcgctgg aggaggcgt ccaataaac cggtgggtg ctgtcatgt cgacttgga ccaagcaaa tgggttttc cggtgatta aggttacga gccgacctg ataataaca aaggggtgg ttttgacgc ttgctcaaa gtaaatata cggctgatg ggtcgaaag caccggatg atttcttgt ggatgattc cggtgtcga ttatcacgt cgaaacaga aaaataagc ccattagta ctgtactta agacgaagg aagcggagg ttgtcattc cggatgttg gtccaaatg attggatta aggtgaatc cggggactg ttggcttct accgcactc agtacgctc cggaccttc ttgccaaat ttattccgg cgatcaaag accggagtt tgcctccac tggaccgac ttggtcttt tggatgatc tgttcgcaa tggtccaag ccggacata cgaacaccg tcgaggtct tgaaactac ttgaagctt tcaccgacg agacggact atacagtct ggtcctcaa tcaacaatg tcttggtta agttaaaca tgttgttga gctataccg actgtgcgg acgacttca aaacgtacc agaaacgca ttttgagca agatttaca aacggttgg ggtggaacc ccaaggcta cggaacgcc atctcgaca ccctcctcc gggggctgg ttttgggcc gcttgtcgt ggctcgatg acgacgaca ccatcgccg aggccaaga aacgtttcg aaggtcacg tcaattcta gtcaaactt taccggcgg atttacgaa gtgcctgct acaaaactg tacttagag cggggggcg aagatgttt acaacacgc ttttgaaat tgtatagat cggtggatt tacacgagg agaaggatc ggatcagtc gagcgctgg gtgcagcga aggatcctg agatcttgt caagggtct tgaagttcg ccatatcgg taaggtaga gttctgagg ggtcttcca ctatctctt tttgtacaa gta
