Status
Please wait ...
Gene information
General information
TC031122
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002353,TC002354 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 356..21373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031122
    iBeetle screen iB_00359

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031122 RA
      MSFLVSFSS RFFLSPLRR VPPIPNLLN ELPEVPLFR 
RRPSPSFRR AQRTRLVHS VAVSVEQPL RGAPCSDRS 
TRKRFCFLA KESAMPTGK PFERLPETV RPKHYVLEL 
VPDLKALVF DGTVAVQIE VVKPTNEIV LNAIDLEIK 
ETTLKGPNV LTPDSTNFS VEDETVTFN FAKPLTPGS 
YTLSMRFKG ELNDKMKGL YRSKYQNQK GEERYAAVT 
QFEATDARR CFPCWDEPA LKATFDITL TVPKDLVAL 
SNMPVKQSK PQGDLIRYD FATTPIMST YLVACVVGE 
YDYVEDKST DGVQVRVYT PRGKKEQGL FALEVATKV 
LPYYKDYFN IAYPLPKID LIAIADFSA GAMENWGLV 
TYRETCLLV DPQNTSAVQ KQWIALVVG HELAHQWFG 
NLVTMEWWT HLWLNEGYA SFVEYLCVD HLFPEYDIW 
TQFVNDTYI RALELDCLK NSHPIEVPV GHPSEIDEI 
FDDISYNKG ASVIRMLHN YIGDEDFRK GMNLYLTRH 
QYKNTFTED LWAALEEAS NKPVGAVMS TWTKQMGFP 
VIKVTSRPD NNKGVVLTL AQSKYTADG SKAPDDFLW 
MIPVSIITS KQKNKPIST VLKTKEAEV VIPDVGPND 
WIKVNPGTV GFYRTQYAP DLLAKFIPA IKDRSLPPL 
DRLGLLDDL FAMVQAGHT NTVEVLKLL EAFTDETDY 
TVWSSINNV LVKLNMLLS YTDCADDFK TYQKRILSK 
IYKRLGWNP KATERHLDT LLRGLVLGR LSWLDDDDT 
IAEAKKRFE GHVNSSQTL PADLRSACY KTVLRAGGE 
DVYNTLLKL YRSVDLHEE KDRISRALG AAKDPEILS 
RVLKFAISV R
    CDS sequence
    • >TC031122 RA
      atgtcattt ctcgtcagt ttttcatcc cgttttttc 
ctgtcgccc ttgcgtaga gtccccccg atcccgaat 
ttattgaat gaattgccg gaagtaccg ttattccgc 
cgccgtccc tccccctcc ttccggcgg gcgcagcgt 
acgcgttta gttcattcg gttgccgtg tcagtggag 
cagccgctt cgtggagca ccttgtagt gaccgtagt 
acgcgtaaa aggttctgt tttctcgcc aaggagtcc 
gcaatgccc actgggaag ccgttcgag aggctgccg 
gagacggtc agacccaaa cactacgtg ctggagctg 
gtgccggac ctgaaggcg ctcgtcttc gatggaacc 
gtcgccgtg caaattgag gttgtgaag ccaacaaac 
gagattgta cttaacgcc atcgactta gaaatcaaa 
gaaacgacc ttaaaaggc cccaatgtc ttaacaccg 
gactcgacc aacttctcg gtcgaagac gaaaccgta 
acatttaat ttcgccaaa ccgctaaca cccggctct 
tacacgctc agtatgcgc ttcaaaggc gaactcaac 
gacaaaatg aaaggtctc taccgaagc aaataccag 
aaccaaaaa ggcgaagaa cgctacgct gccgtcaca 
cagttcgaa gcaaccgac gcccgtcgg tgcttcccc 
tgctgggac gaacccgcc ctcaaagcc accttcgac 
atcacccta accgtaccc aaagatcta gtcgctttg 
agcaacatg cctgtcaaa caatcgaag ccccaaggc 
gacctgatt cgctacgac ttcgcaaca actccaatc 
atgtccacc tatttggtc gcctgtgtg gtgggggag 
tacgattac gtggaggac aaatcaaca gatggggtg 
caagtcagg gtgtacaca ccacggggg aagaaggag 
caagggcta tttgcgctg gaagtggca acgaaagtg 
ctaccctat tataaggat tatttcaat attgcgtac 
cccctgcct aaaatcgat ctgattgcg atcgcagat 
ttcagcgcg ggggctatg gaaaactgg ggcttggtc 
acgtaccgt gagacctgc cttttggtg gacccccag 
aacacctca gctgtgcaa aaacagtgg attgcgctc 
gtagtgggc catgagttg gcccaccaa tggttcggc 
aacttggtc acgatggag tggtggacg catttatgg 
ttgaacgag ggctacgcc tcctttgtg gagtatttg 
tgcgtcgat catttgttc cccgagtac gatatttgg 
acgcagttt gttaatgac acgtatatt cgggctttg 
gagctcgat tgtttgaaa aactcgcac ccgatcgaa 
gttccggtc ggtcatccc tcggaaatc gacgaaatt 
ttcgacgat atcagctat aataagggg gcgtcggtt 
atccgaatg ctgcataat tatatcgga gatgaagat 
tttcgcaaa gggatgaat ttgtactta acgcgacat 
cagtataaa aacacgttt actgaggat ctttgggct 
gcgctggag gaggcgtcc aataaaccg gtgggtgct 
gtcatgtcg acttggacc aagcaaatg ggttttccg 
gtgattaag gttacgagc cgacctgat aataacaaa 
ggggtggtt ttgacgctt gctcaaagt aaatatacg 
gctgatggg tcgaaagca ccggatgat ttcttgtgg 
atgattccg gtgtcgatt atcacgtcg aaacagaaa 
aataagccc attagtact gtacttaag acgaaggaa 
gcggaggtt gtcattccg gatgttggt ccaaatgat 
tggattaag gtgaatccg gggactgtt ggcttctac 
cgcactcag tacgctccg gaccttctt gccaaattt 
attccggcg atcaaagac cggagtttg cctccactg 
gaccgactt ggtcttttg gatgatctg ttcgcaatg 
gtccaagcc ggacatacg aacaccgtc gaggtcttg 
aaactactt gaagctttc accgacgag acggactat 
acagtctgg tcctcaatc aacaatgtc ttggttaag 
ttaaacatg ttgttgagc tataccgac tgtgcggac 
gacttcaaa acgtaccag aaacgcatt ttgagcaag 
atttacaaa cggttgggg tggaacccc aaggctacg 
gaacgccat ctcgacacc ctcctccgg gggctggtt 
ttgggccgc ttgtcgtgg ctcgatgac gacgacacc 
atcgccgag gccaagaaa cgtttcgaa ggtcacgtc 
aattctagt caaacttta ccggcggat ttacgaagt 
gcctgctac aaaactgta cttagagcg gggggcgaa 
gatgtttac aacacgctt ttgaaattg tatagatcg 
gtggattta cacgaggag aaggatcgg atcagtcga 
gcgctgggt gcagcgaag gatcctgag atcttgtca 
agggtcttg aagttcgcc atatcggta aggtag
    mRNA sequence
    • >TC031122 RA
      ggcagctcg accatgtga ccggccccg tttttctca 
attcccgaa gtgggctgg cgtcgaaaa taatgtcat 
ttctcgtca gtttttcat cccgttttt tcctgtcgc 
ccttgcgta gagtccccc cgatcccga atttattga 
atgaattgc cggaagtac cgttattcc gccgccgtc 
cctccccct ccttccggc gggcgcagc gtacgcgtt 
tagttcatt cggttgccg tgtcagtgg agcagccgc 
ttcgtggag caccttgta gtgaccgta gtacgcgta 
aaaggttct gttttctcg ccaaggagt ccgcaatgc 
ccactggga agccgttcg agaggctgc cggagacgg 
tcagaccca aacactacg tgctggagc tggtgccgg 
acctgaagg cgctcgtct tcgatggaa ccgtcgccg 
tgcaaattg aggttgtga agccaacaa acgagattg 
tacttaacg ccatcgact tagaaatca aagaaacga 
ccttaaaag gccccaatg tcttaacac cggactcga 
ccaacttct cggtcgaag acgaaaccg taacattta 
atttcgcca aaccgctaa cacccggct cttacacgc 
tcagtatgc gcttcaaag gcgaactca acgacaaaa 
tgaaaggtc tctaccgaa gcaaatacc agaaccaaa 
aaggcgaag aacgctacg ctgccgtca cacagttcg 
aagcaaccg acgcccgtc ggtgcttcc cctgctggg 
acgaacccg ccctcaaag ccaccttcg acatcaccc 
taaccgtac ccaaagatc tagtcgctt tgagcaaca 
tgcctgtca aacaatcga agccccaag gcgacctga 
ttcgctacg acttcgcaa caactccaa tcatgtcca 
cctatttgg tcgcctgtg tggtggggg agtacgatt 
acgtggagg acaaatcaa cagatgggg tgcaagtca 
gggtgtaca caccacggg ggaagaagg agcaagggc 
tatttgcgc tggaagtgg caacgaaag tgctaccct 
attataagg attatttca atattgcgt accccctgc 
ctaaaatcg atctgattg cgatcgcag atttcagcg 
cgggggcta tggaaaact ggggcttgg tcacgtacc 
gtgagacct gccttttgg tggaccccc agaacacct 
cagctgtgc aaaaacagt ggattgcgc tcgtagtgg 
gccatgagt tggcccacc aatggttcg gcaacttgg 
tcacgatgg agtggtgga cgcatttat ggttgaacg 
agggctacg cctcctttg tggagtatt tgtgcgtcg 
atcatttgt tccccgagt acgatattt ggacgcagt 
ttgttaatg acacgtata ttcgggctt tggagctcg 
attgtttga aaaactcgc acccgatcg aagttccgg 
tcggtcatc cctcggaaa tcgacgaaa ttttcgacg 
atatcagct ataataagg gggcgtcgg ttatccgaa 
tgctgcata attatatcg gagatgaag attttcgca 
aagggatga atttgtact taacgcgac atcagtata 
aaaacacgt ttactgagg atctttggg ctgcgctgg 
aggaggcgt ccaataaac cggtgggtg ctgtcatgt 
cgacttgga ccaagcaaa tgggttttc cggtgatta 
aggttacga gccgacctg ataataaca aaggggtgg 
ttttgacgc ttgctcaaa gtaaatata cggctgatg 
ggtcgaaag caccggatg atttcttgt ggatgattc 
cggtgtcga ttatcacgt cgaaacaga aaaataagc 
ccattagta ctgtactta agacgaagg aagcggagg 
ttgtcattc cggatgttg gtccaaatg attggatta 
aggtgaatc cggggactg ttggcttct accgcactc 
agtacgctc cggaccttc ttgccaaat ttattccgg 
cgatcaaag accggagtt tgcctccac tggaccgac 
ttggtcttt tggatgatc tgttcgcaa tggtccaag 
ccggacata cgaacaccg tcgaggtct tgaaactac 
ttgaagctt tcaccgacg agacggact atacagtct 
ggtcctcaa tcaacaatg tcttggtta agttaaaca 
tgttgttga gctataccg actgtgcgg acgacttca 
aaacgtacc agaaacgca ttttgagca agatttaca 
aacggttgg ggtggaacc ccaaggcta cggaacgcc 
atctcgaca ccctcctcc gggggctgg ttttgggcc 
gcttgtcgt ggctcgatg acgacgaca ccatcgccg 
aggccaaga aacgtttcg aaggtcacg tcaattcta 
gtcaaactt taccggcgg atttacgaa gtgcctgct 
acaaaactg tacttagag cggggggcg aagatgttt 
acaacacgc ttttgaaat tgtatagat cggtggatt 
tacacgagg agaaggatc ggatcagtc gagcgctgg 
gtgcagcga aggatcctg agatcttgt caagggtct 
tgaagttcg ccatatcgg taaggtaga gttctgagg 
ggtcttcca ctatctctt tttgtacaa gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY