Status
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Gene information
General information
TC031122
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002353,TC002354
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 356..21373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031122
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00359

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031122 RA
      MSFLVSFSS RFFLSPLRR VPPIPNLLN ELPEVPLFR 
      RRPSPSFRR AQRTRLVHS VAVSVEQPL RGAPCSDRS 
      TRKRFCFLA KESAMPTGK PFERLPETV RPKHYVLEL 
      VPDLKALVF DGTVAVQIE VVKPTNEIV LNAIDLEIK 
      ETTLKGPNV LTPDSTNFS VEDETVTFN FAKPLTPGS 
      YTLSMRFKG ELNDKMKGL YRSKYQNQK GEERYAAVT 
      QFEATDARR CFPCWDEPA LKATFDITL TVPKDLVAL 
      SNMPVKQSK PQGDLIRYD FATTPIMST YLVACVVGE 
      YDYVEDKST DGVQVRVYT PRGKKEQGL FALEVATKV 
      LPYYKDYFN IAYPLPKID LIAIADFSA GAMENWGLV 
      TYRETCLLV DPQNTSAVQ KQWIALVVG HELAHQWFG 
      NLVTMEWWT HLWLNEGYA SFVEYLCVD HLFPEYDIW 
      TQFVNDTYI RALELDCLK NSHPIEVPV GHPSEIDEI 
      FDDISYNKG ASVIRMLHN YIGDEDFRK GMNLYLTRH 
      QYKNTFTED LWAALEEAS NKPVGAVMS TWTKQMGFP 
      VIKVTSRPD NNKGVVLTL AQSKYTADG SKAPDDFLW 
      MIPVSIITS KQKNKPIST VLKTKEAEV VIPDVGPND 
      WIKVNPGTV GFYRTQYAP DLLAKFIPA IKDRSLPPL 
      DRLGLLDDL FAMVQAGHT NTVEVLKLL EAFTDETDY 
      TVWSSINNV LVKLNMLLS YTDCADDFK TYQKRILSK 
      IYKRLGWNP KATERHLDT LLRGLVLGR LSWLDDDDT 
      IAEAKKRFE GHVNSSQTL PADLRSACY KTVLRAGGE 
      DVYNTLLKL YRSVDLHEE KDRISRALG AAKDPEILS 
      RVLKFAISV R
    CDS sequence
    • >TC031122 RA
      atgtcattt ctcgtcagt ttttcatcc cgttttttc 
      ctgtcgccc ttgcgtaga gtccccccg atcccgaat 
      ttattgaat gaattgccg gaagtaccg ttattccgc 
      cgccgtccc tccccctcc ttccggcgg gcgcagcgt 
      acgcgttta gttcattcg gttgccgtg tcagtggag 
      cagccgctt cgtggagca ccttgtagt gaccgtagt 
      acgcgtaaa aggttctgt tttctcgcc aaggagtcc 
      gcaatgccc actgggaag ccgttcgag aggctgccg 
      gagacggtc agacccaaa cactacgtg ctggagctg 
      gtgccggac ctgaaggcg ctcgtcttc gatggaacc 
      gtcgccgtg caaattgag gttgtgaag ccaacaaac 
      gagattgta cttaacgcc atcgactta gaaatcaaa 
      gaaacgacc ttaaaaggc cccaatgtc ttaacaccg 
      gactcgacc aacttctcg gtcgaagac gaaaccgta 
      acatttaat ttcgccaaa ccgctaaca cccggctct 
      tacacgctc agtatgcgc ttcaaaggc gaactcaac 
      gacaaaatg aaaggtctc taccgaagc aaataccag 
      aaccaaaaa ggcgaagaa cgctacgct gccgtcaca 
      cagttcgaa gcaaccgac gcccgtcgg tgcttcccc 
      tgctgggac gaacccgcc ctcaaagcc accttcgac 
      atcacccta accgtaccc aaagatcta gtcgctttg 
      agcaacatg cctgtcaaa caatcgaag ccccaaggc 
      gacctgatt cgctacgac ttcgcaaca actccaatc 
      atgtccacc tatttggtc gcctgtgtg gtgggggag 
      tacgattac gtggaggac aaatcaaca gatggggtg 
      caagtcagg gtgtacaca ccacggggg aagaaggag 
      caagggcta tttgcgctg gaagtggca acgaaagtg 
      ctaccctat tataaggat tatttcaat attgcgtac 
      cccctgcct aaaatcgat ctgattgcg atcgcagat 
      ttcagcgcg ggggctatg gaaaactgg ggcttggtc 
      acgtaccgt gagacctgc cttttggtg gacccccag 
      aacacctca gctgtgcaa aaacagtgg attgcgctc 
      gtagtgggc catgagttg gcccaccaa tggttcggc 
      aacttggtc acgatggag tggtggacg catttatgg 
      ttgaacgag ggctacgcc tcctttgtg gagtatttg 
      tgcgtcgat catttgttc cccgagtac gatatttgg 
      acgcagttt gttaatgac acgtatatt cgggctttg 
      gagctcgat tgtttgaaa aactcgcac ccgatcgaa 
      gttccggtc ggtcatccc tcggaaatc gacgaaatt 
      ttcgacgat atcagctat aataagggg gcgtcggtt 
      atccgaatg ctgcataat tatatcgga gatgaagat 
      tttcgcaaa gggatgaat ttgtactta acgcgacat 
      cagtataaa aacacgttt actgaggat ctttgggct 
      gcgctggag gaggcgtcc aataaaccg gtgggtgct 
      gtcatgtcg acttggacc aagcaaatg ggttttccg 
      gtgattaag gttacgagc cgacctgat aataacaaa 
      ggggtggtt ttgacgctt gctcaaagt aaatatacg 
      gctgatggg tcgaaagca ccggatgat ttcttgtgg 
      atgattccg gtgtcgatt atcacgtcg aaacagaaa 
      aataagccc attagtact gtacttaag acgaaggaa 
      gcggaggtt gtcattccg gatgttggt ccaaatgat 
      tggattaag gtgaatccg gggactgtt ggcttctac 
      cgcactcag tacgctccg gaccttctt gccaaattt 
      attccggcg atcaaagac cggagtttg cctccactg 
      gaccgactt ggtcttttg gatgatctg ttcgcaatg 
      gtccaagcc ggacatacg aacaccgtc gaggtcttg 
      aaactactt gaagctttc accgacgag acggactat 
      acagtctgg tcctcaatc aacaatgtc ttggttaag 
      ttaaacatg ttgttgagc tataccgac tgtgcggac 
      gacttcaaa acgtaccag aaacgcatt ttgagcaag 
      atttacaaa cggttgggg tggaacccc aaggctacg 
      gaacgccat ctcgacacc ctcctccgg gggctggtt 
      ttgggccgc ttgtcgtgg ctcgatgac gacgacacc 
      atcgccgag gccaagaaa cgtttcgaa ggtcacgtc 
      aattctagt caaacttta ccggcggat ttacgaagt 
      gcctgctac aaaactgta cttagagcg gggggcgaa 
      gatgtttac aacacgctt ttgaaattg tatagatcg 
      gtggattta cacgaggag aaggatcgg atcagtcga 
      gcgctgggt gcagcgaag gatcctgag atcttgtca 
      agggtcttg aagttcgcc atatcggta aggtag
    mRNA sequence
    • >TC031122 RA
      ggcagctcg accatgtga ccggccccg tttttctca 
      attcccgaa gtgggctgg cgtcgaaaa taatgtcat 
      ttctcgtca gtttttcat cccgttttt tcctgtcgc 
      ccttgcgta gagtccccc cgatcccga atttattga 
      atgaattgc cggaagtac cgttattcc gccgccgtc 
      cctccccct ccttccggc gggcgcagc gtacgcgtt 
      tagttcatt cggttgccg tgtcagtgg agcagccgc 
      ttcgtggag caccttgta gtgaccgta gtacgcgta 
      aaaggttct gttttctcg ccaaggagt ccgcaatgc 
      ccactggga agccgttcg agaggctgc cggagacgg 
      tcagaccca aacactacg tgctggagc tggtgccgg 
      acctgaagg cgctcgtct tcgatggaa ccgtcgccg 
      tgcaaattg aggttgtga agccaacaa acgagattg 
      tacttaacg ccatcgact tagaaatca aagaaacga 
      ccttaaaag gccccaatg tcttaacac cggactcga 
      ccaacttct cggtcgaag acgaaaccg taacattta 
      atttcgcca aaccgctaa cacccggct cttacacgc 
      tcagtatgc gcttcaaag gcgaactca acgacaaaa 
      tgaaaggtc tctaccgaa gcaaatacc agaaccaaa 
      aaggcgaag aacgctacg ctgccgtca cacagttcg 
      aagcaaccg acgcccgtc ggtgcttcc cctgctggg 
      acgaacccg ccctcaaag ccaccttcg acatcaccc 
      taaccgtac ccaaagatc tagtcgctt tgagcaaca 
      tgcctgtca aacaatcga agccccaag gcgacctga 
      ttcgctacg acttcgcaa caactccaa tcatgtcca 
      cctatttgg tcgcctgtg tggtggggg agtacgatt 
      acgtggagg acaaatcaa cagatgggg tgcaagtca 
      gggtgtaca caccacggg ggaagaagg agcaagggc 
      tatttgcgc tggaagtgg caacgaaag tgctaccct 
      attataagg attatttca atattgcgt accccctgc 
      ctaaaatcg atctgattg cgatcgcag atttcagcg 
      cgggggcta tggaaaact ggggcttgg tcacgtacc 
      gtgagacct gccttttgg tggaccccc agaacacct 
      cagctgtgc aaaaacagt ggattgcgc tcgtagtgg 
      gccatgagt tggcccacc aatggttcg gcaacttgg 
      tcacgatgg agtggtgga cgcatttat ggttgaacg 
      agggctacg cctcctttg tggagtatt tgtgcgtcg 
      atcatttgt tccccgagt acgatattt ggacgcagt 
      ttgttaatg acacgtata ttcgggctt tggagctcg 
      attgtttga aaaactcgc acccgatcg aagttccgg 
      tcggtcatc cctcggaaa tcgacgaaa ttttcgacg 
      atatcagct ataataagg gggcgtcgg ttatccgaa 
      tgctgcata attatatcg gagatgaag attttcgca 
      aagggatga atttgtact taacgcgac atcagtata 
      aaaacacgt ttactgagg atctttggg ctgcgctgg 
      aggaggcgt ccaataaac cggtgggtg ctgtcatgt 
      cgacttgga ccaagcaaa tgggttttc cggtgatta 
      aggttacga gccgacctg ataataaca aaggggtgg 
      ttttgacgc ttgctcaaa gtaaatata cggctgatg 
      ggtcgaaag caccggatg atttcttgt ggatgattc 
      cggtgtcga ttatcacgt cgaaacaga aaaataagc 
      ccattagta ctgtactta agacgaagg aagcggagg 
      ttgtcattc cggatgttg gtccaaatg attggatta 
      aggtgaatc cggggactg ttggcttct accgcactc 
      agtacgctc cggaccttc ttgccaaat ttattccgg 
      cgatcaaag accggagtt tgcctccac tggaccgac 
      ttggtcttt tggatgatc tgttcgcaa tggtccaag 
      ccggacata cgaacaccg tcgaggtct tgaaactac 
      ttgaagctt tcaccgacg agacggact atacagtct 
      ggtcctcaa tcaacaatg tcttggtta agttaaaca 
      tgttgttga gctataccg actgtgcgg acgacttca 
      aaacgtacc agaaacgca ttttgagca agatttaca 
      aacggttgg ggtggaacc ccaaggcta cggaacgcc 
      atctcgaca ccctcctcc gggggctgg ttttgggcc 
      gcttgtcgt ggctcgatg acgacgaca ccatcgccg 
      aggccaaga aacgtttcg aaggtcacg tcaattcta 
      gtcaaactt taccggcgg atttacgaa gtgcctgct 
      acaaaactg tacttagag cggggggcg aagatgttt 
      acaacacgc ttttgaaat tgtatagat cggtggatt 
      tacacgagg agaaggatc ggatcagtc gagcgctgg 
      gtgcagcga aggatcctg agatcttgt caagggtct 
      tgaagttcg ccatatcgg taaggtaga gttctgagg 
      ggtcttcca ctatctctt tttgtacaa gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY