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Gene information
General information
TC002346 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452276.1: 91732..101621
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002346
    iBeetle screen iB_00358

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 9 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002346 RA
      MEGAASETS EAQPPTHEE GVPGDLNSA SELTESDKN 
      VASDKELTW EKIAAEFTE EEEIMEEEA ALAQVAQDV 
      APPPETEVV QETVINTVT EEKVEPLET PKIENESEP 
      LATETEPSV EPMTMSTEP SAEPIETST EPTSMSTEP 
      LSEPPASEQ TEMPNDEVV EHPTPELEK TTEFVEITE 
      LKPEEPIVD EVVEDLEYT VVEAEEVET HEIIEEHLA 
      QTEPSSETV VIINPDQSQ TEITIEHME ESGTADENS 
      QEYREEIYV LTKDNESVD DPDYTTTKE KKPRKQRGR 
      KPISDIPLH VLGRDITKP TEGVSNGGR SMPKPRLGV 
      KVPYKNLTS QIVSKEEIE KEIMERFKL KQEQNSTNS 
      GDILFARKL TQRLAKKLI PSEKDKADA KPQEATTSE 
      KNDKNSSEI KNNSDLIAI LEGEDEDME VKKKQTEEE 
      KKEMTKNTE KEIALQQLK ELPGKNKIF KSRSEEEKP 
      ANPVLKPKP PSPQKPSPE KKLVEAEPR LKTEMVIKT 
      YTRKRKSFD LESILPAKK TVVATQIKS EDGGPPSDV 
      YITKSSRVI KKKVIWDPD EVPAKSPIK SPKSAESPV 
      KPVEKEKPQ QEKKVASPV KKIVKTSSP PVKKPKRLT 
      EVDKLLMDE GAVNMLYDV KTNEETSPD KQKKKTTKS 
      VISLDKAHK ELMSKTSVI KNDLQQNTS GQKSLRKKE 
      QGSPAKKEA KVATPSGVT RKKSKDSAR SSVHSPPSS 
      PPYNAAEAS RIIRRHSSS SFSSNEEMD DEEIEEVEE 
      RVTRKKAAA AAESPKKKL KKTTEKPKE TTTKNNNND 
      VKANHVDKT SVGKYKSFT ATKRNKLVS INLLSIDGK 
      CYLSEEVLK ELTACLKEF GEDDSCHVV SITADNSES 
      FCEGLDYRV LVAEDEGGR KKRASELVA LVKDFLRCL 
      LHFPKVLVA GIQGDCVGL GVTMLPLFD MVIASDTST 
      FSTPYSRLG LVPEAGFLL TVPHLSSNG LASELLFAS 
      QTIKADDAF RRGLVTRLC WPEKYQQEL KSMLGQISS 
      QSRQSIVAA KQQLRQNIL QSTESALSA VCKVLVENW 
      TSPECQKNF NFPK
    CDS sequence
    • >TC002346 RA
      atggagggc gcagcgagt gagacaagc gaggcgcag 
      cccccgacg cacgaagag ggcgttcct ggggacttg 
      aacagtgct agtgaactc actgaaagt gataaaaac 
      gtggctagt gacaaggag ttaacgtgg gagaaaatc 
      gcagctgaa ttcactgaa gaagaggag attatggag 
      gaggaggcg gccctggct caagtagca caagacgtg 
      gctcctccg ccggagacg gaagttgta caggagact 
      gtgataaat actgtgact gaagaaaag gttgaacca 
      ctggagacc ccgaagatt gaaaacgaa agtgaacct 
      ttggcaaca gaaactgaa ccttcagtt gaacctatg 
      acaatgtcc actgaacct tcagctgaa cctatagag 
      acgtcaact gaacctacg tcgatgtca actgaacct 
      ctaagtgaa cctccggca agtgaacaa accgagatg 
      cctaatgat gaagttgtt gaacacccg actccagag 
      ctcgaaaaa acgacagaa tttgtcgaa attacggag 
      ttaaaacct gaggaaccg atagttgac gaagttgtg 
      gaggacttg gagtatact gttgtcgag gctgaggaa 
      gttgaaact catgaaata atcgaggaa catttagct 
      caaactgag cccagcagc gaaactgtg gttataatt 
      aatcctgac cagtctcag actgaaatc actatagaa 
      catatggag gaaagtgga acagcggat gagaattcg 
      caggagtac agagaagaa atttacgtg ttgacaaaa 
      gataatgaa agtgttgat gatccggat tatacaacg 
      acgaaagaa aagaagcct aggaagcaa agggggcgc 
      aaacccatc agtgacatt cctctccac gttttggga 
      cgtgatata accaaaccg actgaaggt gtgtcaaac 
      gggggccgc tctatgccc aaaccaaga ctaggtgtg 
      aaggttcct tacaagaat ttaacaagt cagatagtt 
      tcaaaagaa gaaatcgag aaggaaatt atggagcgt 
      ttcaaacta aaacaggag caaaactcg accaacagt 
      ggcgatatt ttattcgcc cgtaaacta acacaacgt 
      ctagccaaa aaactaatc cctagtgag aaagacaaa 
      gcagatgcc aagccccaa gaagccaca acttccgag 
      aaaaacgat aaaaactca tccgaaatc aagaacaac 
      tccgatttg attgcaatt cttgaaggc gaagacgaa 
      gatatggaa gtgaagaag aaacaaacc gaagaagag 
      aagaaagaa atgaccaaa aacacagaa aaggaaatt 
      gccttacaa caattgaag gaactgccc gggaaaaac 
      aaaattttc aagagccgg agcgaggag gaaaaaccg 
      gcaaatccg gtcctgaag ccaaaacca ccatcgcca 
      caaaaacct agccccgag aaaaaacta gtcgaggcc 
      gaacctagg cttaagacc gaaatggtc attaaaacc 
      tacacccga aaacgtaaa tcattcgat ttggagtcg 
      attttgcct gcgaaaaaa accgttgtg gcaacacag 
      atcaaatcg gaagatggt gggcctcca agtgacgta 
      tacataacg aaaagttca cgtgtgatt aagaagaaa 
      gtcatttgg gacccggac gaagtcccg gctaaaagt 
      ccgatcaag tcaccgaaa agcgccgaa tcgccggtt 
      aaaccggtc gaaaaggag aaaccgcaa caagagaag 
      aaagttgct tcgcctgtt aaaaaaatc gtaaagacg 
      tcgtcacca ccggttaaa aaaccgaaa cgcttgact 
      gaagttgat aagttacta atggacgaa ggggccgtt 
      aatatgctc tacgacgtc aagacgaac gaagaaacg 
      agccctgat aaacaaaag aagaagact acgaagtca 
      gttatcagt ttagataag gcgcacaaa gaactgatg 
      agtaagact agcgttatc aagaacgac cttcagcag 
      aatacaagt ggtcagaag tcattacgt aagaaagaa 
      cagggttcg cctgcgaag aaagaggcc aaagtggcg 
      acgcctagc ggcgtgacg cgaaaaaaa tcgaaggat 
      tccgctcgg agttcggtt catagtcct ccctcgtct 
      cctccatac aatgcggcg gaagcttcg cgtattatc 
      cgtcggcat tcgagcagt tcgttttcg agcaacgaa 
      gaaatggat gatgaagag attgaagag gttgaagag 
      agggtgacg cggaagaaa gcagcagca gctgccgaa 
      tcgccgaag aagaagttg aaaaagacg acggaaaaa 
      ccgaaagaa acgacgacg aaaaataat aataatgat 
      gttaaggcg aatcatgtg gataagacg agtgtgggg 
      aagtataag tcgttcacg gcaactaaa agaaataaa 
      ttggtgtcg attaatttg ctcagtatc gatgggaaa 
      tgttatttg agcgaagag gttttgaag gagttgacg 
      gcctgtttg aaggagttt ggagaggat gatagttgt 
      catgtggtg tcgataaca gcggataat agcgagtcg 
      ttttgtgaa gggttggat tatagggtg ttggtggct 
      gaagatgag ggtggacgg aagaagaga gccagcgag 
      ctcgtggcg ttagttaag gatttccta aggtgcctt 
      ttacacttc cccaaagtc ctagtggct ggaatccaa 
      ggggactgc gtgggttta ggcgtgaca atgttaccg 
      cttttcgac atggtgata gcgagtgac acctcgacg 
      tttagtacg ccgtactcg cgccttggt ttagttccc 
      gaagcaggt ttcctcctt acagtccca catctgagc 
      agcaacggc ctggcgagc gaattgttg tttgcgtcg 
      cagaccatc aaagccgac gatgcgttc agaaggggg 
      ctcgtgacg aggctttgc tggcccgag aagtaccaa 
      caggagctg aagagtatg ctggggcag atttcgagc 
      cagtcgaga cagagtatt gtagcggcg aagcagcag 
      ctacgtcag aatatcttg cagagtact gagtcggcc 
      ttgagtgca gtttgtaaa gttctggtc gagaactgg 
      actagtccg gagtgtcag aagaatttt aattttccg 
      aaatag
    mRNA sequence
    • >TC002346 RA
      aaaccaaga tggcgtttt tcgtaatag tggacgcac 
      cactcgctc gactttatt ttcccgaaa attaacaaa 
      ttctaagga aaatatcat gaaataaag gctgaggag 
      tctcccgtc tggttgtat aatcccttg aggtccatg 
      aaataacgt ttgtttgga aatatcgac gcggaaacg 
      ttatctaat aaggtggtg aaatggagg gcgcagcga 
      gtgagacaa gcgaggcgc agcccccga cgcacgaag 
      agggcgttc ctggggact tgaacagtg ctagtgaac 
      tcactgaaa gtgataaaa acgtggcta gtgacaagg 
      agttaacgt gggagaaaa tcgcagctg aattcactg 
      aagaagagg agattatgg aggaggagg cggccctgg 
      ctcaagtag cacaagacg tggctcctc cgccggaga 
      cggaagttg tacaggaga ctgtgataa atactgtga 
      ctgaagaaa aggttgaac cactggaga ccccgaaga 
      ttgaaaacg aaagtgaac ctttggcaa cagaaactg 
      aaccttcag ttgaaccta tgacaatgt ccactgaac 
      cttcagctg aacctatag agacgtcaa ctgaaccta 
      cgtcgatgt caactgaac ctctaagtg aacctccgg 
      caagtgaac aaaccgaga tgcctaatg atgaagttg 
      ttgaacacc cgactccag agctcgaaa aaacgacag 
      aatttgtcg aaattacgg agttaaaac ctgaggaac 
      cgatagttg acgaagttg tggaggact tggagtata 
      ctgttgtcg aggctgagg aagttgaaa ctcatgaaa 
      taatcgagg aacatttag ctcaaactg agcccagca 
      gcgaaactg tggttataa ttaatcctg accagtctc 
      agactgaaa tcactatag aacatatgg aggaaagtg 
      gaacagcgg atgagaatt cgcaggagt acagagaag 
      aaatttacg tgttgacaa aagataatg aaagtgttg 
      atgatccgg attatacaa cgacgaaag aaaagaagc 
      ctaggaagc aaagggggc gcaaaccca tcagtgaca 
      ttcctctcc acgttttgg gacgtgata taaccaaac 
      cgactgaag gtgtgtcaa acgggggcc gctctatgc 
      ccaaaccaa gactaggtg tgaaggttc cttacaaga 
      atttaacaa gtcagatag tttcaaaag aagaaatcg 
      agaaggaaa ttatggagc gtttcaaac taaaacagg 
      agcaaaact cgaccaaca gtggcgata ttttattcg 
      cccgtaaac taacacaac gtctagcca aaaaactaa 
      tccctagtg agaaagaca aagcagatg ccaagcccc 
      aagaagcca caacttccg agaaaaacg ataaaaact 
      catccgaaa tcaagaaca actccgatt tgattgcaa 
      ttcttgaag gcgaagacg aagatatgg aagtgaaga 
      agaaacaaa ccgaagaag agaagaaag aaatgacca 
      aaaacacag aaaaggaaa ttgccttac aacaattga 
      aggaactgc ccgggaaaa acaaaattt tcaagagcc 
      ggagcgagg aggaaaaac cggcaaatc cggtcctga 
      agccaaaac caccatcgc cacaaaaac ctagccccg 
      agaaaaaac tagtcgagg ccgaaccta ggcttaaga 
      ccgaaatgg tcattaaaa cctacaccc gaaaacgta 
      aatcattcg atttggagt cgattttgc ctgcgaaaa 
      aaaccgttg tggcaacac agatcaaat cggaagatg 
      gtgggcctc caagtgacg tatacataa cgaaaagtt 
      cacgtgtga ttaagaaga aagtcattt gggacccgg 
      acgaagtcc cggctaaaa gtccgatca agtcaccga 
      aaagcgccg aatcgccgg ttaaaccgg tcgaaaagg 
      agaaaccgc aacaagaga agaaagttg cttcgcctg 
      ttaaaaaaa tcgtaaaga cgtcgtcac caccggtta 
      aaaaaccga aacgcttga ctgaagttg ataagttac 
      taatggacg aaggggccg ttaatatgc tctacgacg 
      tcaagacga acgaagaaa cgagccctg ataaacaaa 
      agaagaaga ctacgaagt cagttatca gtttagata 
      aggcgcaca aagaactga tgagtaaga ctagcgtta 
      tcaagaacg accttcagc agaatacaa gtggtcaga 
      agtcattac gtaagaaag aacagggtt cgcctgcga 
      agaaagagg ccaaagtgg cgacgccta gcggcgtga 
      cgcgaaaaa aatcgaagg attccgctc ggagttcgg 
      ttcatagtc ctccctcgt ctcctccat acaatgcgg 
      cggaagctt cgcgtatta tccgtcggc attcgagca 
      gttcgtttt cgagcaacg aagaaatgg atgatgaag 
      agattgaag aggttgaag agagggtga cgcggaaga 
      aagcagcag cagctgccg aatcgccga agaagaagt 
      tgaaaaaga cgacggaaa aaccgaaag aaacgacga 
      cgaaaaata ataataatg atgttaagg cgaatcatg 
      tggataaga cgagtgtgg ggaagtata agtcgttca 
      cggcaacta aaagaaata aattggtgt cgattaatt 
      tgctcagta tcgatggga aatgttatt tgagcgaag 
      aggttttga aggagttga cggcctgtt tgaaggagt 
      ttggagagg atgatagtt gtcatgtgg tgtcgataa 
      cagcggata atagcgagt cgttttgtg aagggttgg 
      attataggg tgttggtgg ctgaagatg agggtggac 
      ggaagaaga gagccagcg agctcgtgg cgttagtta 
      aggatttcc taaggtgcc ttttacact tccccaaag 
      tcctagtgg ctggaatcc aaggggact gcgtgggtt 
      taggcgtga caatgttac cgcttttcg acatggtga 
      tagcgagtg acacctcga cgtttagta cgccgtact 
      cgcgccttg gtttagttc ccgaagcag gtttcctcc 
      ttacagtcc cacatctga gcagcaacg gcctggcga 
      gcgaattgt tgtttgcgt cgcagacca tcaaagccg 
      acgatgcgt tcagaaggg ggctcgtga cgaggcttt 
      gctggcccg agaagtacc aacaggagc tgaagagta 
      tgctggggc agatttcga gccagtcga gacagagta 
      ttgtagcgg cgaagcagc agctacgtc agaatatct 
      tgcagagta ctgagtcgg ccttgagtg cagtttgta 
      aagttctgg tcgagaact ggactagtc cggagtgtc 
      agaagaatt ttaattttc cgaaataga cgtagatta 
      gagggatga aacttgagg aaactatct atggtacaa 
      actgttctg tttcatttt agattaagt gactaaatt 
      actgaatgt acattttcg aaggtgtgt cacttggaa 
      aataatatt ttttatatt ttcataaat agagtaagt 
      cccatgttt aggatatcc attaggttg tacaaaatc 
      aaacaaagc gtagaaaat tcaattcca ctttcatgc 
      acgttttta tgatgtcga tatcgtgtt ttccaaaga 
      ttggtgcaa tttttgtcg tcatgggag agatgtttg 
      ggtttttac agtgttgag acttatggt taatatttt 
      taaataaaa catctgaat taacatgtc tggattgtt 
      gatttcgtc gacttccgt ttcttcgat tttgctttt 
      actttagtt ttaattttt ctgttatta atagtagat 
      tcgttttag tattatctt tatgcaata tattatgta 
      acagagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY