| General information | |||
| TC002346 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015452276.1: 91732..101621 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002346 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00358 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 9 (19 dpi)
- >TC002346 RA
MEGAASETS EAQPPTHEE GVPGDLNSA SELTESDKN VASDKELTW EKIAAEFTE EEEIMEEEA ALAQVAQDV APPPETEVV QETVINTVT EEKVEPLET PKIENESEP LATETEPSV EPMTMSTEP SAEPIETST EPTSMSTEP LSEPPASEQ TEMPNDEVV EHPTPELEK TTEFVEITE LKPEEPIVD EVVEDLEYT VVEAEEVET HEIIEEHLA QTEPSSETV VIINPDQSQ TEITIEHME ESGTADENS QEYREEIYV LTKDNESVD DPDYTTTKE KKPRKQRGR KPISDIPLH VLGRDITKP TEGVSNGGR SMPKPRLGV KVPYKNLTS QIVSKEEIE KEIMERFKL KQEQNSTNS GDILFARKL TQRLAKKLI PSEKDKADA KPQEATTSE KNDKNSSEI KNNSDLIAI LEGEDEDME VKKKQTEEE KKEMTKNTE KEIALQQLK ELPGKNKIF KSRSEEEKP ANPVLKPKP PSPQKPSPE KKLVEAEPR LKTEMVIKT YTRKRKSFD LESILPAKK TVVATQIKS EDGGPPSDV YITKSSRVI KKKVIWDPD EVPAKSPIK SPKSAESPV KPVEKEKPQ QEKKVASPV KKIVKTSSP PVKKPKRLT EVDKLLMDE GAVNMLYDV KTNEETSPD KQKKKTTKS VISLDKAHK ELMSKTSVI KNDLQQNTS GQKSLRKKE QGSPAKKEA KVATPSGVT RKKSKDSAR SSVHSPPSS PPYNAAEAS RIIRRHSSS SFSSNEEMD DEEIEEVEE RVTRKKAAA AAESPKKKL KKTTEKPKE TTTKNNNND VKANHVDKT SVGKYKSFT ATKRNKLVS INLLSIDGK CYLSEEVLK ELTACLKEF GEDDSCHVV SITADNSES FCEGLDYRV LVAEDEGGR KKRASELVA LVKDFLRCL LHFPKVLVA GIQGDCVGL GVTMLPLFD MVIASDTST FSTPYSRLG LVPEAGFLL TVPHLSSNG LASELLFAS QTIKADDAF RRGLVTRLC WPEKYQQEL KSMLGQISS QSRQSIVAA KQQLRQNIL QSTESALSA VCKVLVENW TSPECQKNF NFPK
- >TC002346 RA
atggagggc gcagcgagt gagacaagc gaggcgcag cccccgacg cacgaagag ggcgttcct ggggacttg aacagtgct agtgaactc actgaaagt gataaaaac gtggctagt gacaaggag ttaacgtgg gagaaaatc gcagctgaa ttcactgaa gaagaggag attatggag gaggaggcg gccctggct caagtagca caagacgtg gctcctccg ccggagacg gaagttgta caggagact gtgataaat actgtgact gaagaaaag gttgaacca ctggagacc ccgaagatt gaaaacgaa agtgaacct ttggcaaca gaaactgaa ccttcagtt gaacctatg acaatgtcc actgaacct tcagctgaa cctatagag acgtcaact gaacctacg tcgatgtca actgaacct ctaagtgaa cctccggca agtgaacaa accgagatg cctaatgat gaagttgtt gaacacccg actccagag ctcgaaaaa acgacagaa tttgtcgaa attacggag ttaaaacct gaggaaccg atagttgac gaagttgtg gaggacttg gagtatact gttgtcgag gctgaggaa gttgaaact catgaaata atcgaggaa catttagct caaactgag cccagcagc gaaactgtg gttataatt aatcctgac cagtctcag actgaaatc actatagaa catatggag gaaagtgga acagcggat gagaattcg caggagtac agagaagaa atttacgtg ttgacaaaa gataatgaa agtgttgat gatccggat tatacaacg acgaaagaa aagaagcct aggaagcaa agggggcgc aaacccatc agtgacatt cctctccac gttttggga cgtgatata accaaaccg actgaaggt gtgtcaaac gggggccgc tctatgccc aaaccaaga ctaggtgtg aaggttcct tacaagaat ttaacaagt cagatagtt tcaaaagaa gaaatcgag aaggaaatt atggagcgt ttcaaacta aaacaggag caaaactcg accaacagt ggcgatatt ttattcgcc cgtaaacta acacaacgt ctagccaaa aaactaatc cctagtgag aaagacaaa gcagatgcc aagccccaa gaagccaca acttccgag aaaaacgat aaaaactca tccgaaatc aagaacaac tccgatttg attgcaatt cttgaaggc gaagacgaa gatatggaa gtgaagaag aaacaaacc gaagaagag aagaaagaa atgaccaaa aacacagaa aaggaaatt gccttacaa caattgaag gaactgccc gggaaaaac aaaattttc aagagccgg agcgaggag gaaaaaccg gcaaatccg gtcctgaag ccaaaacca ccatcgcca caaaaacct agccccgag aaaaaacta gtcgaggcc gaacctagg cttaagacc gaaatggtc attaaaacc tacacccga aaacgtaaa tcattcgat ttggagtcg attttgcct gcgaaaaaa accgttgtg gcaacacag atcaaatcg gaagatggt gggcctcca agtgacgta tacataacg aaaagttca cgtgtgatt aagaagaaa gtcatttgg gacccggac gaagtcccg gctaaaagt ccgatcaag tcaccgaaa agcgccgaa tcgccggtt aaaccggtc gaaaaggag aaaccgcaa caagagaag aaagttgct tcgcctgtt aaaaaaatc gtaaagacg tcgtcacca ccggttaaa aaaccgaaa cgcttgact gaagttgat aagttacta atggacgaa ggggccgtt aatatgctc tacgacgtc aagacgaac gaagaaacg agccctgat aaacaaaag aagaagact acgaagtca gttatcagt ttagataag gcgcacaaa gaactgatg agtaagact agcgttatc aagaacgac cttcagcag aatacaagt ggtcagaag tcattacgt aagaaagaa cagggttcg cctgcgaag aaagaggcc aaagtggcg acgcctagc ggcgtgacg cgaaaaaaa tcgaaggat tccgctcgg agttcggtt catagtcct ccctcgtct cctccatac aatgcggcg gaagcttcg cgtattatc cgtcggcat tcgagcagt tcgttttcg agcaacgaa gaaatggat gatgaagag attgaagag gttgaagag agggtgacg cggaagaaa gcagcagca gctgccgaa tcgccgaag aagaagttg aaaaagacg acggaaaaa ccgaaagaa acgacgacg aaaaataat aataatgat gttaaggcg aatcatgtg gataagacg agtgtgggg aagtataag tcgttcacg gcaactaaa agaaataaa ttggtgtcg attaatttg ctcagtatc gatgggaaa tgttatttg agcgaagag gttttgaag gagttgacg gcctgtttg aaggagttt ggagaggat gatagttgt catgtggtg tcgataaca gcggataat agcgagtcg ttttgtgaa gggttggat tatagggtg ttggtggct gaagatgag ggtggacgg aagaagaga gccagcgag ctcgtggcg ttagttaag gatttccta aggtgcctt ttacacttc cccaaagtc ctagtggct ggaatccaa ggggactgc gtgggttta ggcgtgaca atgttaccg cttttcgac atggtgata gcgagtgac acctcgacg tttagtacg ccgtactcg cgccttggt ttagttccc gaagcaggt ttcctcctt acagtccca catctgagc agcaacggc ctggcgagc gaattgttg tttgcgtcg cagaccatc aaagccgac gatgcgttc agaaggggg ctcgtgacg aggctttgc tggcccgag aagtaccaa caggagctg aagagtatg ctggggcag atttcgagc cagtcgaga cagagtatt gtagcggcg aagcagcag ctacgtcag aatatcttg cagagtact gagtcggcc ttgagtgca gtttgtaaa gttctggtc gagaactgg actagtccg gagtgtcag aagaatttt aattttccg aaatag
- >TC002346 RA
aaaccaaga tggcgtttt tcgtaatag tggacgcac cactcgctc gactttatt ttcccgaaa attaacaaa ttctaagga aaatatcat gaaataaag gctgaggag tctcccgtc tggttgtat aatcccttg aggtccatg aaataacgt ttgtttgga aatatcgac gcggaaacg ttatctaat aaggtggtg aaatggagg gcgcagcga gtgagacaa gcgaggcgc agcccccga cgcacgaag agggcgttc ctggggact tgaacagtg ctagtgaac tcactgaaa gtgataaaa acgtggcta gtgacaagg agttaacgt gggagaaaa tcgcagctg aattcactg aagaagagg agattatgg aggaggagg cggccctgg ctcaagtag cacaagacg tggctcctc cgccggaga cggaagttg tacaggaga ctgtgataa atactgtga ctgaagaaa aggttgaac cactggaga ccccgaaga ttgaaaacg aaagtgaac ctttggcaa cagaaactg aaccttcag ttgaaccta tgacaatgt ccactgaac cttcagctg aacctatag agacgtcaa ctgaaccta cgtcgatgt caactgaac ctctaagtg aacctccgg caagtgaac aaaccgaga tgcctaatg atgaagttg ttgaacacc cgactccag agctcgaaa aaacgacag aatttgtcg aaattacgg agttaaaac ctgaggaac cgatagttg acgaagttg tggaggact tggagtata ctgttgtcg aggctgagg aagttgaaa ctcatgaaa taatcgagg aacatttag ctcaaactg agcccagca gcgaaactg tggttataa ttaatcctg accagtctc agactgaaa tcactatag aacatatgg aggaaagtg gaacagcgg atgagaatt cgcaggagt acagagaag aaatttacg tgttgacaa aagataatg aaagtgttg atgatccgg attatacaa cgacgaaag aaaagaagc ctaggaagc aaagggggc gcaaaccca tcagtgaca ttcctctcc acgttttgg gacgtgata taaccaaac cgactgaag gtgtgtcaa acgggggcc gctctatgc ccaaaccaa gactaggtg tgaaggttc cttacaaga atttaacaa gtcagatag tttcaaaag aagaaatcg agaaggaaa ttatggagc gtttcaaac taaaacagg agcaaaact cgaccaaca gtggcgata ttttattcg cccgtaaac taacacaac gtctagcca aaaaactaa tccctagtg agaaagaca aagcagatg ccaagcccc aagaagcca caacttccg agaaaaacg ataaaaact catccgaaa tcaagaaca actccgatt tgattgcaa ttcttgaag gcgaagacg aagatatgg aagtgaaga agaaacaaa ccgaagaag agaagaaag aaatgacca aaaacacag aaaaggaaa ttgccttac aacaattga aggaactgc ccgggaaaa acaaaattt tcaagagcc ggagcgagg aggaaaaac cggcaaatc cggtcctga agccaaaac caccatcgc cacaaaaac ctagccccg agaaaaaac tagtcgagg ccgaaccta ggcttaaga ccgaaatgg tcattaaaa cctacaccc gaaaacgta aatcattcg atttggagt cgattttgc ctgcgaaaa aaaccgttg tggcaacac agatcaaat cggaagatg gtgggcctc caagtgacg tatacataa cgaaaagtt cacgtgtga ttaagaaga aagtcattt gggacccgg acgaagtcc cggctaaaa gtccgatca agtcaccga aaagcgccg aatcgccgg ttaaaccgg tcgaaaagg agaaaccgc aacaagaga agaaagttg cttcgcctg ttaaaaaaa tcgtaaaga cgtcgtcac caccggtta aaaaaccga aacgcttga ctgaagttg ataagttac taatggacg aaggggccg ttaatatgc tctacgacg tcaagacga acgaagaaa cgagccctg ataaacaaa agaagaaga ctacgaagt cagttatca gtttagata aggcgcaca aagaactga tgagtaaga ctagcgtta tcaagaacg accttcagc agaatacaa gtggtcaga agtcattac gtaagaaag aacagggtt cgcctgcga agaaagagg ccaaagtgg cgacgccta gcggcgtga cgcgaaaaa aatcgaagg attccgctc ggagttcgg ttcatagtc ctccctcgt ctcctccat acaatgcgg cggaagctt cgcgtatta tccgtcggc attcgagca gttcgtttt cgagcaacg aagaaatgg atgatgaag agattgaag aggttgaag agagggtga cgcggaaga aagcagcag cagctgccg aatcgccga agaagaagt tgaaaaaga cgacggaaa aaccgaaag aaacgacga cgaaaaata ataataatg atgttaagg cgaatcatg tggataaga cgagtgtgg ggaagtata agtcgttca cggcaacta aaagaaata aattggtgt cgattaatt tgctcagta tcgatggga aatgttatt tgagcgaag aggttttga aggagttga cggcctgtt tgaaggagt ttggagagg atgatagtt gtcatgtgg tgtcgataa cagcggata atagcgagt cgttttgtg aagggttgg attataggg tgttggtgg ctgaagatg agggtggac ggaagaaga gagccagcg agctcgtgg cgttagtta aggatttcc taaggtgcc ttttacact tccccaaag tcctagtgg ctggaatcc aaggggact gcgtgggtt taggcgtga caatgttac cgcttttcg acatggtga tagcgagtg acacctcga cgtttagta cgccgtact cgcgccttg gtttagttc ccgaagcag gtttcctcc ttacagtcc cacatctga gcagcaacg gcctggcga gcgaattgt tgtttgcgt cgcagacca tcaaagccg acgatgcgt tcagaaggg ggctcgtga cgaggcttt gctggcccg agaagtacc aacaggagc tgaagagta tgctggggc agatttcga gccagtcga gacagagta ttgtagcgg cgaagcagc agctacgtc agaatatct tgcagagta ctgagtcgg ccttgagtg cagtttgta aagttctgg tcgagaact ggactagtc cggagtgtc agaagaatt ttaattttc cgaaataga cgtagatta gagggatga aacttgagg aaactatct atggtacaa actgttctg tttcatttt agattaagt gactaaatt actgaatgt acattttcg aaggtgtgt cacttggaa aataatatt ttttatatt ttcataaat agagtaagt cccatgttt aggatatcc attaggttg tacaaaatc aaacaaagc gtagaaaat tcaattcca ctttcatgc acgttttta tgatgtcga tatcgtgtt ttccaaaga ttggtgcaa tttttgtcg tcatgggag agatgtttg ggtttttac agtgttgag acttatggt taatatttt taaataaaa catctgaat taacatgtc tggattgtt gatttcgtc gacttccgt ttcttcgat tttgctttt actttagtt ttaattttt ctgttatta atagtagat tcgttttag tattatctt tatgcaata tattatgta acagagt
