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Gene information
General information
TC002330 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452129.1: 0..1293
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002330
    iBeetle screen iB_00356

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 3

    ovary size decreased - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002330 RA
      MVSKRKKKY TTGEGAQFM TRKAALKKL QLSLNDFRR 
      ICILKGIYP REPRNRKRA QKGQSGIKT LYHVKDIQF 
      LLHEPMIWR LRDYKIFNK KVGRARAVK DFESLKKYL 
      NNHPTLKLD HIVKERYPT FLDALRDLD DCLTLCFLF 
      STFPSIPHV PRDQSALCQ RLTIEFLHA VIEAKALRK 
      VFISIKGYY YQAEIKGET ITWIVPHHF AFEPQSKAE 
      VDFKLMSTF VEFYSIMLG FVNFRLYHQ LNLYYPPKF 
      TNLSQTDSE KEIVDEGIF VSERVAALN FPLSKSTNS 
      AIEDEVEID NFNTEDSPE KIEEARIEA EK
    CDS sequence
    • >TC002330 RA
      atggtttcc aaacgcaaa aagaagtac acaaccggc 
      gagggtgcc caattcatg acccgcaaa gcggccctc 
      aaaaagctc caattatcc ctcaacgac ttccgccgc 
      atctgcatc cttaagggc atctacccc cgtgagccc 
      cgcaaccgc aaacgtgcc cagaagggc cagtccggt 
      atcaaaact ctctaccac gtcaaagac atccaattt 
      ctgttacac gagcccatg atttggcgc cttcgcgac 
      tacaaaatc ttcaacaag aaagtcggg cgtgcccga 
      gctgttaaa gacttcgaa tcgttgaag aaatacctg 
      aataaccac ccaactttg aaactcgac cacattgtc 
      aaagagcga taccccacg tttttggac gcgttgcgc 
      gacctggac gactgtctc actttgtgt ttcttgttc 
      agtacgttt ccgtcaatc cctcacgtc ccccgcgac 
      cagagtgcg ctgtgtcag aggctcaca attgagttt 
      ttacacgcg gtgatcgag gccaaggcc ctaaggaaa 
      gtttttatt tcaatcaag gggtattac taccaagcg 
      gaaatcaaa ggtgagacc attacgtgg attgtgccc 
      caccatttc gcgttcgag ccccagagc aaggccgaa 
      gtcgatttt aagttaatg tcgactttt gtcgagttt 
      tactcgatt atgttgggt tttgtcaat ttccggctc 
      taccaccaa ttaaatctt tattatccg cccaaattt 
      acgaatttg agccaaaca gacagtgag aaagaaatc 
      gtagacgag gggattttt gtgtcagaa cgtgtggca 
      gcccttaat ttccctcta tccaaaagt acgaattca 
      gcaattgag gacgaagtc gaaattgat aatttcaac 
      actgaggac agtccggag aaaatcgaa gaggcgcga 
      attgaggct gaaaaataa
    mRNA sequence
    • >TC002330 RA
      ctgccaaca cccctaatt taaagattg gactcgtcc 
      gagacgagt tttcgtctc tgcgcactc ctaccaccg 
      tggacgatc tgtcatttc gcggactct aacctcaaa 
      acaacacgt gttggcatt tctccagtt gttaacgtt 
      ttagtgtat tattttgtt aaaacatgg tttccaaac 
      gcaaaaaga agtacacaa ccggcgagg gtgcccaat 
      tcatgaccc gcaaagcgg ccctcaaaa agctccaat 
      tatccctca acgacttcc gccgcatct gcatcctta 
      agggcatct acccccgtg agccccgca accgcaaac 
      gtgcccaga agggccagt ccggtatca aaactctct 
      accacgtca aagacatcc aatttctgt tacacgagc 
      ccatgattt ggcgccttc gcgactaca aaatcttca 
      acaagaaag tcgggcgtg cccgagctg ttaaagact 
      tcgaatcgt tgaagaaat acctgaata accacccaa 
      ctttgaaac tcgaccaca ttgtcaaag agcgatacc 
      ccacgtttt tggacgcgt tgcgcgacc tggacgact 
      gtctcactt tgtgtttct tgttcagta cgtttccgt 
      caatccctc acgtccccc gcgaccaga gtgcgctgt 
      gtcagaggc tcacaattg agtttttac acgcggtga 
      tcgaggcca aggccctaa ggaaagttt ttatttcaa 
      tcaaggggt attactacc aagcggaaa tcaaaggtg 
      agaccatta cgtggattg tgccccacc atttcgcgt 
      tcgagcccc agagcaagg ccgaagtcg attttaagt 
      taatgtcga cttttgtcg agttttact cgattatgt 
      tgggttttg tcaatttcc ggctctacc accaattaa 
      atctttatt atccgccca aatttacga atttgagcc 
      aaacagaca gtgagaaag aaatcgtag acgagggga 
      tttttgtgt cagaacgtg tggcagccc ttaatttcc 
      ctctatcca aaagtacga attcagcaa ttgaggacg 
      aagtcgaaa ttgataatt tcaacactg aggacagtc 
      cggagaaaa tcgaagagg cgcgaattg aggctgaaa 
      aataaaaaa ttgaagaca cttttcaag ggactgaag 
      ttctttttg aatcgagaa gttccagag aacctctcg 
      tgtttatta tccgttgtt tcgggggcg agtctcgtg 
      ggataagtt acttttgtg g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY