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Gene information
General information
TC002325 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1547: 4772..9987
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002325
      iBeetle screen iB_00355
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002325 RA
        MPPKRNAQL EIFNLQSKA GSFESQLNH FENYIRSID 
        DSNVNHQLI IQLELRISN IQSLYDKYE TIYNELKNY 
        DAATSITSL NDFGDSFYN ILAAAKSII STFESNKQN 
        LPQYLPQDA NNSCGAFNG AYEEQVKLA NLDLPTFDG 
        DYMQWAGFK DSFTTLIHN SKRIISKTN KFHYLKLSL 
        KGAALKVIE NMQANDSNY DIAWDLLHQ RFDNKQLIV 
        KSHLDAIFE LPTVNKDSC QSLRELHDN LNKNLRALK 
        NLGQPVEYW DTIIIHIIL NKLDSNSKR AFAEFKRQC 
        EFPTLDDLN NFIKDRCIV LEQLSFKTK AEQPAQINK 
        PKRTPPYQN HYAYASTTN NDNTNSGKS CPLCKADHF 
        LHSCDEFYK MTVIERFNF ILLATIVVN IKDVKGITH 
        KVRCLMDGG SQSNFITSK LCKKLGLVT TPINYTVLG 
        INSYPSNIN KQVKICISS SMTKFECNL HCLVIQKIT 
        EKLPIISFD KNILNLPPD VVLADPNFN VSSEIDLLM 
        GASLAFQIL RGEPISLGN DNLPIIHNT QFGWVVAGN 
        LITEAISQN TSFSFCNTN MSDNVSNED LQRNLSQFW 
        EIEEDVKLL SSLSKENQL CETFFSETV SREPSGKFI 
        VKMPLKDNH VFLGDSEQM ALRRFYSLE QKLAKNPQL 
        KTQYSQFIN EYKEMNHMS LVSKQFSDL EPGYYVPHH 
        AVYKAESLT TKLRVVFDA SAKTDSGLS LNDVQMAGP 
        NLQNDLSSI LLRFRKHAV VLTADVMKM YRMIHIHPE 
        QRKFLRIFW RDNSNEQLQ LYELNTVTY GTASAPYLA 
        IKCLNVLAD ENKETFPKT CEIIKGDFY VDDVLTGAD 
        AVEEVLQIQ NELSTLFWA KQGKMFIQE LFKQKLSWD 
        EPVSGELKS KWFALFQGF MAVDELKIP RHVLLNNSV 
        SVDIHGFSD ASEKAYGAC IYCRSTDQL GNVSVKLLC 
        SKSRVAPLK VLTIPRLEL CGALLLARL INKAEESLQ 
        IIINKRYCW CDSKIVLSW IHSDPSRWR IFVSNRVTQ 
        IQNLTNGHT WLYIETHNN PADVLSKGI EPKELQNYC 
        LWWDGPSFL HDSSELWNN KSIACQTLT SEEQKCKVV 
        VTVSVNDFD VFNKFCSTF SCFKKLIKS FAWWTRFKN 
        RLQKRPVKS SKTLDLEEY VAAETTLIR LLQEQCFDK 
        DLYDLKSGT LSSSSKIKS LHPFLDTNG VIRVGGRLG 
        HSTYSFSKK HPILLPNKH KLTDLIATD CHLKLLHVG 
        PQGLLSALR ETYWPIAGR NLARKVYRN CVTCFKANP 
        QPLQHIMGE LPSCRVKQT FPFYCVGID YAGPFHLKD 
        RTTRNPKIV KAYVCLFVC MAVKAVHIE VVSDLTSEA 
        FLACLKRFI SRRGKPKDI FSDNGLNFV GAANELREL 
        YVFLKSEST QNKILEFLT SEKISWHFI PPRAPHMGG 
        LWEAGIKSM KFHLTRIVG NASLTFEEL NTIVTQVEA 
        ILNSRPLVP LSSNPDDLS VLSPGHFII GRAMVALPD 
        YDYQEIPEN KLSRFQRLQ RIVQHFWSR WTREYICEL 
        QNRSKWRNN SLNALKKGS LVIVRDDQT SPQQWRLGR 
        VLDLHPGHD GIVRVVTIK FATQIAKRP VAKLCVLPI 
        GESV
      CDS sequence
      • >TC002325 RA
        atgcctccg aaaagaaat gctcaattg gaaattttc 
        aatttgcaa agtaaggcc ggatctttt gaaagccaa 
        ctaaatcat ttcgaaaat tatattcgt tcaatcgat 
        gattcaaat gtgaatcat cagttaatt atacaactt 
        gagctgcgt atttctaac attcagtca ttatacgat 
        aaatatgaa acgatatat aacgaatta aaaaactat 
        gacgcagca acctctatc acgtcttta aatgatttt 
        ggagacagt ttttataac attttagca gcagctaag 
        agcattatc tcgacattt gagtcaaat aaacaaaat 
        ttacctcaa tatttacct caagatgca aacaactct 
        tgtggggct tttaatggc gcttacgaa gagcaagtc 
        aaattagct aatttagat ttgcccacc tttgatggc 
        gattacatg caatgggca ggattcaag gactcattt 
        acgaccttg atccacaac agtaaacga ataatttca 
        aaaacgaat aaatttcat tacttaaag ttaagccta 
        aaaggtgct gccttgaag gtcatcgaa aatatgcaa 
        gcaaacgac tcaaattat gatatcgca tgggacttg 
        ttgcatcaa cgctttgat aataagcaa ttaatcgtt 
        aaaagtcat ttggacgcg atttttgaa ttaccgaca 
        gttaataaa gactcatgc cagtctcta cgggaactg 
        catgataat ttaaacaaa aatcttaga gctctcaaa 
        aatttgggc caacccgta gaatactgg gatactata 
        attatacac ataattctc aataaactg gattcaaat 
        tctaaacgc gcttttgca gaatttaaa agacagtgc 
        gaatttccg acattggac gatttaaat aactttatc 
        aaagatcgt tgtattgtt ttagaacaa ctgagtttt 
        aagactaag gcagaacaa cctgctcaa attaacaaa 
        ccaaaacga actccacca tatcaaaac cattatgca 
        tatgctagt acgacaaat aacgacaat accaattct 
        ggcaaatca tgtccttta tgtaaggct gatcatttt 
        ctgcattca tgcgacgaa ttttacaaa atgactgta 
        atcgagcgt ttcaacttt attcttttg gcaactatt 
        gtagtaaac ataaaagac gtcaaagga atcactcat 
        aaagttcgt tgcctgatg gacggaggc tcacaaagt 
        aattttatt acatcaaaa ttatgcaaa aaattagga 
        cttgtaact acacctatt aattacact gtgttgggc 
        ataaactct tatccatcg aacattaat aagcaagtc 
        aaaatctgt atttcatcc tcaatgact aaatttgaa 
        tgcaactta cattgcttg gttattcaa aaaattacg 
        gaaaaatta ccaatcata tcatttgat aaaaatatt 
        ttaaatcta ccccctgat gtagttttg gcagatcca 
        aatttcaat gtttctagc gaaatcgat cttttaatg 
        ggtgcgtca ctagctttt cagatctta cgtggtgaa 
        cccatatca ttgggtaat gataatttg cctattatt 
        cataatact caatttggt tgggtggta gcaggaaat 
        ttaatcaca gaagctata tcacaaaat acgtctttc 
        tctttttgt aacacgaat atgtctgat aatgtctca 
        aacgaagac cttcaaaga aatttgagc caattttgg 
        gaaatcgaa gaagatgta aaactactt tcttctctt 
        tctaaagaa aatcagtta tgcgaaaca ttcttttct 
        gaaacagta tcacgcgaa ccttccggg aaatttatt 
        gttaaaatg cctctgaaa gataaccat gttttcttg 
        ggtgattca gaacaaatg gcacttcga cgattttac 
        agtctggag caaaagtta gccaaaaat cctcaattg 
        aaaacacaa tattcgcag tttatcaat gagtataaa 
        gaaatgaat catatgtct ttggtttca aagcaattt 
        tcggactta gaaccaggt tattacgtg ccacatcat 
        gctgtttat aaagctgaa agtcttaca actaaattg 
        cgtgttgtt ttcgacgca tcagctaaa actgattcg 
        ggcctatcg ttaaatgat gtgcaaatg gccggtcct 
        aatttacag aatgatctt tcttctatt ttattgaga 
        tttagaaaa cacgctgtt gtcctaacg gcagacgtg 
        atgaaaatg tatcgaatg attcatatc catcccgaa 
        caaagaaag tttttgcgt atattttgg cgagacaat 
        tcaaacgaa caacttcag ttatacgaa cttaacacg 
        gtaacgtat ggtactgcg agcgcaccg tatttagct 
        attaaatgt ctaaatgtg ctagcagat gaaaataaa 
        gaaacgttt ccaaaaacg tgcgagatt attaaaggt 
        gacttttat gttgatgac gtcctgacg ggagcagac 
        gcggttgaa gaagtttta caaatccaa aatgaacta 
        tcaactcta ttttgggcc aagcaggga aaaatgttt 
        attcaagag ctatttaaa caaaaactc tcatgggat 
        gagcctgtt tcgggtgaa ctcaaatca aagtggttc 
        gctttgttt caaggtttt atggctgtt gacgaatta 
        aaaatacct cggcatgtt cttttaaat aatagtgtt 
        tcagttgac attcatgga ttttctgat gcttcagaa 
        aaggcttac ggtgcatgt atttattgt cgatcaact 
        gaccaactg ggaaacgtg tcggttaaa ctattgtgt 
        tcaaaaagt cgcgtggca ccattaaaa gtactaact 
        atacctcgt ttagaattg tgtggggca ttattactg 
        gctcgactt attaataag gcagaagaa tcactacaa 
        ataattatc aacaaacgg tattgttgg tgcgattca 
        aaaatagtt ctctcttgg atacattcg gatccgagt 
        cgctggaga atttttgtg tctaacaga gtaacacaa 
        attcaaaat cttaccaat ggacatact tggctatac 
        attgagaca cacaataat cccgcagat gttctgtcc 
        aaggggatc gagcccaaa gaattacaa aattattgt 
        ttgtggtgg gacggtcca tcttttctt catgactca 
        tctgagctt tggaacaat aaatcaatt gcctgtcag 
        actctaact tcggaagaa cagaaatgc aaggtcgtt 
        gtcacagtt tcggttaac gattttgat gtatttaat 
        aaattttgt tcaacattt tcatgtttc aaaaaacta 
        attaaatca tttgcgtgg tggacaaga tttaaaaat 
        cgtctgcag aaaaggcct gttaagtct tcaaaaaca 
        ttagacttg gaagaatat gttgctgca gaaactact 
        ttgatacgt ctgttgcaa gagcaatgt tttgataag 
        gatttatat gatttaaaa tctggaact ttgtcgtct 
        agcagtaaa attaaaagc ttgcatcca tttttagac 
        accaatggt gtaataagg gttggtggc agattgggc 
        cattcaaca tattcattt tctaaaaaa catcccatt 
        ttacttccg aataaacat aaattaacg gatttaatt 
        gccactgat tgtcatctg aaacttcta catgttggg 
        cctcaaggt ttattaagt gccttgcgg gaaacttat 
        tggccaatt gctggtaga aatttggct agaaaggtt 
        tatcggaac tgtgtcact tgttttaaa gctaatcct 
        cagccttta caacatata atgggtgaa ttgcctagt 
        tgtagagtc aaacaaacg tttccgttt tattgtgta 
        ggaatagat tacgctggt ccgtttcac ttgaaagat 
        aggacaact agaaatcca aaaattgtt aaagcatat 
        gtttgccta tttgtctgt atggctgtc aaagcagta 
        catatcgaa gttgttagt gatttaact agcgaggcc 
        tttcttgca tgtttaaaa cgcttcatc tcgcggaga 
        ggtaaacct aaagatatt ttcagtgac aatgggtta 
        aatttcgtg ggtgccgcc aatgagttg cgggagttg 
        tatgtattt ttaaagtca gaatcgact caaaacaaa 
        attttggaa tttttgaca tcagaaaaa attagttgg 
        catttcata cctcctaga gcccctcat atgggaggt 
        ctttgggag gcaggaatt aaatctatg aagtttcac 
        ttaacgaga attgttggg aatgccagt cttactttc 
        gaagaattg aacactata gtcactcaa gtagaggca 
        atacttaat tcacgaccc cttgttcct ttatcctca 
        aatcctgat gacctatca gtgctgtca ccgggtcat 
        tttatcatt ggacgagca atggtggca ttaccagac 
        tatgactac caagaaata ccggaaaat aaattatca 
        cgatttcaa cgacttcag cgaatcgta caacacttc 
        tggtcccgt tggacccgt gaatatata tgtgagctg 
        caaaaccga agcaagtgg aggaacaat tcactcaat 
        gcactgaag aaaggctcc ttggttata gtgcgggac 
        gaccagacc tctccgcag caatggaga ctaggcagg 
        gtgttggat ctacatcca ggacatgat ggtattgtg 
        cgggtggtg acgattaaa tttgcaacg caaattgca 
        aaaaggcct gttgctaag ttgtgtgtc ttaccgatt 
        ggtgagagt gtttga
      mRNA sequence
      • >TC002325 RA
        atgcctccg aaaagaaat gctcaattg gaaattttc 
        aatttgcaa agtaaggcc ggatctttt gaaagccaa 
        ctaaatcat ttcgaaaat tatattcgt tcaatcgat 
        gattcaaat gtgaatcat cagttaatt atacaactt 
        gagctgcgt atttctaac attcagtca ttatacgat 
        aaatatgaa acgatatat aacgaatta aaaaactat 
        gacgcagca acctctatc acgtcttta aatgatttt 
        ggagacagt ttttataac attttagca gcagctaag 
        agcattatc tcgacattt gagtcaaat aaacaaaat 
        ttacctcaa tatttacct caagatgca aacaactct 
        tgtggggct tttaatggc gcttacgaa gagcaagtc 
        aaattagct aatttagat ttgcccacc tttgatggc 
        gattacatg caatgggca ggattcaag gactcattt 
        acgaccttg atccacaac agtaaacga ataatttca 
        aaaacgaat aaatttcat tacttaaag ttaagccta 
        aaaggtgct gccttgaag gtcatcgaa aatatgcaa 
        gcaaacgac tcaaattat gatatcgca tgggacttg 
        ttgcatcaa cgctttgat aataagcaa ttaatcgtt 
        aaaagtcat ttggacgcg atttttgaa ttaccgaca 
        gttaataaa gactcatgc cagtctcta cgggaactg 
        catgataat ttaaacaaa aatcttaga gctctcaaa 
        aatttgggc caacccgta gaatactgg gatactata 
        attatacac ataattctc aataaactg gattcaaat 
        tctaaacgc gcttttgca gaatttaaa agacagtgc 
        gaatttccg acattggac gatttaaat aactttatc 
        aaagatcgt tgtattgtt ttagaacaa ctgagtttt 
        aagactaag gcagaacaa cctgctcaa attaacaaa 
        ccaaaacga actccacca tatcaaaac cattatgca 
        tatgctagt acgacaaat aacgacaat accaattct 
        ggcaaatca tgtccttta tgtaaggct gatcatttt 
        ctgcattca tgcgacgaa ttttacaaa atgactgta 
        atcgagcgt ttcaacttt attcttttg gcaactatt 
        gtagtaaac ataaaagac gtcaaagga atcactcat 
        aaagttcgt tgcctgatg gacggaggc tcacaaagt 
        aattttatt acatcaaaa ttatgcaaa aaattagga 
        cttgtaact acacctatt aattacact gtgttgggc 
        ataaactct tatccatcg aacattaat aagcaagtc 
        aaaatctgt atttcatcc tcaatgact aaatttgaa 
        tgcaactta cattgcttg gttattcaa aaaattacg 
        gaaaaatta ccaatcata tcatttgat aaaaatatt 
        ttaaatcta ccccctgat gtagttttg gcagatcca 
        aatttcaat gtttctagc gaaatcgat cttttaatg 
        ggtgcgtca ctagctttt cagatctta cgtggtgaa 
        cccatatca ttgggtaat gataatttg cctattatt 
        cataatact caatttggt tgggtggta gcaggaaat 
        ttaatcaca gaagctata tcacaaaat acgtctttc 
        tctttttgt aacacgaat atgtctgat aatgtctca 
        aacgaagac cttcaaaga aatttgagc caattttgg 
        gaaatcgaa gaagatgta aaactactt tcttctctt 
        tctaaagaa aatcagtta tgcgaaaca ttcttttct 
        gaaacagta tcacgcgaa ccttccggg aaatttatt 
        gttaaaatg cctctgaaa gataaccat gttttcttg 
        ggtgattca gaacaaatg gcacttcga cgattttac 
        agtctggag caaaagtta gccaaaaat cctcaattg 
        aaaacacaa tattcgcag tttatcaat gagtataaa 
        gaaatgaat catatgtct ttggtttca aagcaattt 
        tcggactta gaaccaggt tattacgtg ccacatcat 
        gctgtttat aaagctgaa agtcttaca actaaattg 
        cgtgttgtt ttcgacgca tcagctaaa actgattcg 
        ggcctatcg ttaaatgat gtgcaaatg gccggtcct 
        aatttacag aatgatctt tcttctatt ttattgaga 
        tttagaaaa cacgctgtt gtcctaacg gcagacgtg 
        atgaaaatg tatcgaatg attcatatc catcccgaa 
        caaagaaag tttttgcgt atattttgg cgagacaat 
        tcaaacgaa caacttcag ttatacgaa cttaacacg 
        gtaacgtat ggtactgcg agcgcaccg tatttagct 
        attaaatgt ctaaatgtg ctagcagat gaaaataaa 
        gaaacgttt ccaaaaacg tgcgagatt attaaaggt 
        gacttttat gttgatgac gtcctgacg ggagcagac 
        gcggttgaa gaagtttta caaatccaa aatgaacta 
        tcaactcta ttttgggcc aagcaggga aaaatgttt 
        attcaagag ctatttaaa caaaaactc tcatgggat 
        gagcctgtt tcgggtgaa ctcaaatca aagtggttc 
        gctttgttt caaggtttt atggctgtt gacgaatta 
        aaaatacct cggcatgtt cttttaaat aatagtgtt 
        tcagttgac attcatgga ttttctgat gcttcagaa 
        aaggcttac ggtgcatgt atttattgt cgatcaact 
        gaccaactg ggaaacgtg tcggttaaa ctattgtgt 
        tcaaaaagt cgcgtggca ccattaaaa gtactaact 
        atacctcgt ttagaattg tgtggggca ttattactg 
        gctcgactt attaataag gcagaagaa tcactacaa 
        ataattatc aacaaacgg tattgttgg tgcgattca 
        aaaatagtt ctctcttgg atacattcg gatccgagt 
        cgctggaga atttttgtg tctaacaga gtaacacaa 
        attcaaaat cttaccaat ggacatact tggctatac 
        attgagaca cacaataat cccgcagat gttctgtcc 
        aaggggatc gagcccaaa gaattacaa aattattgt 
        ttgtggtgg gacggtcca tcttttctt catgactca 
        tctgagctt tggaacaat aaatcaatt gcctgtcag 
        actctaact tcggaagaa cagaaatgc aaggtcgtt 
        gtcacagtt tcggttaac gattttgat gtatttaat 
        aaattttgt tcaacattt tcatgtttc aaaaaacta 
        attaaatca tttgcgtgg tggacaaga tttaaaaat 
        cgtctgcag aaaaggcct gttaagtct tcaaaaaca 
        ttagacttg gaagaatat gttgctgca gaaactact 
        ttgatacgt ctgttgcaa gagcaatgt tttgataag 
        gatttatat gatttaaaa tctggaact ttgtcgtct 
        agcagtaaa attaaaagc ttgcatcca tttttagac 
        accaatggt gtaataagg gttggtggc agattgggc 
        cattcaaca tattcattt tctaaaaaa catcccatt 
        ttacttccg aataaacat aaattaacg gatttaatt 
        gccactgat tgtcatctg aaacttcta catgttggg 
        cctcaaggt ttattaagt gccttgcgg gaaacttat 
        tggccaatt gctggtaga aatttggct agaaaggtt 
        tatcggaac tgtgtcact tgttttaaa gctaatcct 
        cagccttta caacatata atgggtgaa ttgcctagt 
        tgtagagtc aaacaaacg tttccgttt tattgtgta 
        ggaatagat tacgctggt ccgtttcac ttgaaagat 
        aggacaact agaaatcca aaaattgtt aaagcatat 
        gtttgccta tttgtctgt atggctgtc aaagcagta 
        catatcgaa gttgttagt gatttaact agcgaggcc 
        tttcttgca tgtttaaaa cgcttcatc tcgcggaga 
        ggtaaacct aaagatatt ttcagtgac aatgggtta 
        aatttcgtg ggtgccgcc aatgagttg cgggagttg 
        tatgtattt ttaaagtca gaatcgact caaaacaaa 
        attttggaa tttttgaca tcagaaaaa attagttgg 
        catttcata cctcctaga gcccctcat atgggaggt 
        ctttgggag gcaggaatt aaatctatg aagtttcac 
        ttaacgaga attgttggg aatgccagt cttactttc 
        gaagaattg aacactata gtcactcaa gtagaggca 
        atacttaat tcacgaccc cttgttcct ttatcctca 
        aatcctgat gacctatca gtgctgtca ccgggtcat 
        tttatcatt ggacgagca atggtggca ttaccagac 
        tatgactac caagaaata ccggaaaat aaattatca 
        cgatttcaa cgacttcag cgaatcgta caacacttc 
        tggtcccgt tggacccgt gaatatata tgtgagctg 
        caaaaccga agcaagtgg aggaacaat tcactcaat 
        gcactgaag aaaggctcc ttggttata gtgcgggac 
        gaccagacc tctccgcag caatggaga ctaggcagg 
        gtgttggat ctacatcca ggacatgat ggtattgtg 
        cgggtggtg acgattaaa tttgcaacg caaattgca 
        aaaaggcct gttgctaag ttgtgtgtc ttaccgatt 
        ggtgagagt gtttga