Status
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Gene information
General information
TC002311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 752668..767484
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002311
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00354

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002311 RA
      MFRIGKYFS PQRESLMRT YATKKIIAI RREDQSVWE 
      RRAPFSPSH VRKLVKTGV KIIVQPSNR RAYPMQSYL 
      NAGAIIQED ISEANIIFG VKQVPIGQL IPNKTYCIF 
      SHTIKAQES NMPLLDAIL EKRIRLIDY EKLMDEKGQ 
      RVVAFGKMA GIAGTVNIF HGLGLRLLA LGHHTPFMH 
      IGPAHNYRN SSMAKQAVR DAGYEIALG LMPKSIGPL 
      TVVFTGSGN VSQGSQEVF QELPHEYVA PESLKKAAE 
      HGSLNKVYA CEVRRRHYL ERAEGGGFD PVEYEEHPE 
      RYISTFSKK IAPYASIII NGIYWAVNS PKLLTIPDA 
      KHLLRPAHT PWLPTSIGA PALPHRMLA ICDISADPG 
      GSIEFMNEC TTIDTPFCL YDADRNKDT KSFNGPGVL 
      VCSIDNMPT QIPRESTDF FGDLLFPYV QDIIKSNAQ 
      QPLESHSFC PAVDGAIIA SNGKLTPNY EYIQELRKS 
      AIKSRHKAS SESSSVEKQ VVIVGAGRV AAPLVEYLH 
      RDKSVGITV ACEQKDLSD NLARAFPGI ESLYLNAVE 
      NSNTLEEIV KKADVAVSI LPANLHHIV AQACIKEGT 
      HMVTASYMS QEMKNLHRE AVDAGITVL NEVGLDPGI 
      DHLLALECI QEVQQAGGR ITSFESFCG GLPAPEFSD 
      NPLRYKFSW SPRGALLNT LSSARYLSK GQIVEISAG 
      GELMNTTKT LDFLPGFNL EGFPNRDST IYGKYYGIE 
      DALTVLRGT IRYTGFAQA ARMLQFLGL LDPEPHPAL 
      HAQGPEITW RMLICNLLG LENHNIFYD NLKNQIVER 
      TGSEFSVDL LEELGLLDE HGVIKCGSP LDTLTHYLS 
      TKLALEKHE RDLVILRHE IGINWPDNR KEIRGVNFV 
      VYGDANGYS AMAKTVGYP AAIATKMIL DGEIQDRGM 
      ILPFAPEIY RTILSRLRN EGLNSTETS KFI
    CDS sequence
    • >TC002311 RA
      atgttccga attggaaag tatttttcg cctcagcgt 
      gaatcgctg atgaggacg tatgccacg aagaaaata 
      atcgccatc cgccgcgaa gaccaaagc gtctgggaa 
      cgtcgcgcc cctttctca ccctcacac gttcgaaaa 
      ttagtcaaa acaggcgtc aaaatcatc gtccaaccg 
      agcaatcgt cgcgcctac cccatgcaa tcctaccta 
      aacgcagga gccataatt caagaggac atctctgaa 
      gcaaacata atttttgga gtaaaacaa gtaccaatc 
      gggcaactt atccccaac aaaacctac tgcatattc 
      tcacacact atcaaagcc caagagtca aacatgccc 
      ctccttgac gccatcctc gaaaaacgc ataagactg 
      atcgattac gaaaaactg atggatgag aaaggccaa 
      agggttgtg gcgtttggc aaaatggcc ggaattgca 
      ggaaccgtg aacattttt cacggacta ggtttgaga 
      ctactggcc ttaggacac catacacct tttatgcac 
      attggacca gctcacaac taccgtaac tcaagcatg 
      gccaagcaa gctgttcga gatgctgga tatgaaata 
      gcacttggt ttgatgcca aaatcaatc ggaccgtta 
      actgtggtg ttcactgga tcaggaaac gtgtctcag 
      ggctcccaa gaagtgttc caagaacta ccacatgaa 
      tacgtagct cccgagtct ttgaaaaaa gcggcagaa 
      catggcagt ttaaataaa gtctatgct tgtgaggtg 
      aggagaaga cactacttg gaacgtgca gaaggtggt 
      ggttttgac ccagtcgag tatgaggag catccagaa 
      cgttacatt tccacattt agtaaaaaa attgcaccg 
      tatgcttcc atcatcatt aacggaatt tactgggct 
      gttaacagt cccaaactt ttgactatt ccggacgct 
      aaacatttg ttaaggcct gcacacacg ccttggctg 
      cctacttca ataggtgcg ccagctttg cctcatcga 
      atgttggcg atttgtgat atttcggct gatcctgga 
      ggttcgatt gagtttatg aatgagtgt acgacgatt 
      gatacgccg ttttgtctc tacgatgct gataggaat 
      aaagatacg aagagtttt aacgggcct ggggttctg 
      gtctgcagt attgataat atgccgacg caaattcca 
      agggaaagt actgacttt tttggggat ttgttgttc 
      ccgtatgtg caagatatt ataaaatct aatgcgcag 
      caaccgctc gagagtcat agtttttgt ccagctgtt 
      gatggggca atcatcgcc agcaacggc aaactcact 
      cccaactac gaatacatc caagaattg cgaaaatca 
      gcaattaaa agcaggcac aaagcaagt agcgagtcc 
      tcaagcgtg gaaaaacag gtcgttatc gtgggcgca 
      ggacgggta gctgcacct ttggtggaa tacctccac 
      agggacaaa tccgtcggt atcaccgtc gcgtgcgaa 
      cagaaagac ttgagcgat aacttagct cgagctttc 
      cccggcatc gaaagcctc tacttgaac gctgtggaa 
      aactccaat actttggaa gaaattgtc aaaaaagca 
      gatgttgct gtttcaatc cttccagct aacttgcac 
      cacattgtg gcccaggca tgtatcaag gaaggcacc 
      catatggtc acggctagt tacatgagc caggaaatg 
      aagaatttg caccgagaa gcagtcgat gccggtatc 
      acagtgctt aatgaagta ggtttggac ccaggaatc 
      gatcatttg ttggctcta gaatgcatc caagaagtg 
      caacaagct ggaggacga atcacctca tttgagtcg 
      ttctgtgga gggctcccg gcaccagaa ttcagcgat 
      aatcctttg aggtacaag ttttcgtgg tcaccaaga 
      ggcgctctt ttgaacacg ttatcgtca gcccggtat 
      ttgagcaag ggccaaatt gttgaaatt agcgcagga 
      ggagagttg atgaataca accaaaacg cttgatttt 
      ctccctggt ttcaacctt gaaggtttc cccaatcgg 
      gacagcaca atttatgga aaatactac ggaattgaa 
      gacgcttta actgttttg agaggcacc atcagatac 
      acgggtttt gcccaagct gcaagaatg ttgcaattt 
      ttggggctg ctagaccca gaaccacat ccagcactg 
      catgctcaa gggcccgaa atcacctgg agaatgctc 
      atttgcaac cttttggga ctcgaaaac cacaacatc 
      ttctacgat aacctcaag aaccaaatt gttgaacgg 
      acaggttcc gagttttcc gtcgatttg cttgaagaa 
      ttaggtttg ttggacgaa catggtgtg attaaatgt 
      ggatctcca ttggatact ctcactcat tacctctca 
      actaaattg gcgttggaa aaacatgag cgtgatttg 
      gtgattttg cgtcacgag atcggtatt aattggcca 
      gacaacagg aaagagatc agaggtgtt aattttgtt 
      gtttatgga gatgctaat ggctattct gccatggca 
      aagactgtt gggtatcca gcggctatt gcgacgaaa 
      atgattctg gatggtgaa atacaagat aggggaatg 
      attcttcca tttgccccc gaaatttac agaacgatt 
      ttgtccaga ttgcgaaat gagggcttg aatagcact 
      gagacatcg aaatttatt taa
    mRNA sequence
    • >TC002311 RA
      taattgcaa aattatctg cttatttct gatcggaaa 
      acaaaaata cttaaagtg ttcgataaa aatcaggtc 
      aaggcttta gtttattga aaatgctcg gtgggagct 
      gtgacacac tccataatc aagaaccga tcgcaggaa 
      gtaaagaag aaaggaaca aaccagagg aaaaaagca 
      aataggaac actgggtct gaataattt ttggaaaat 
      tttcaaaat gttccgaat tggaaagta tttttcgcc 
      tcagcgtga atcgctgat gaggacgta tgccacgaa 
      gaaaataat cgccatccg ccgcgaaga ccaaagcgt 
      ctgggaacg tcgcgcccc tttctcacc ctcacacgt 
      tcgaaaatt agtcaaaac aggcgtcaa aatcatcgt 
      ccaaccgag caatcgtcg cgcctaccc catgcaatc 
      ctacctaaa cgcaggagc cataattca agaggacat 
      ctctgaagc aaacataat ttttggagt aaaacaagt 
      accaatcgg gcaacttat ccccaacaa aacctactg 
      catattctc acacactat caaagccca agagtcaaa 
      catgcccct ccttgacgc catcctcga aaaacgcat 
      aagactgat cgattacga aaaactgat ggatgagaa 
      aggccaaag ggttgtggc gtttggcaa aatggccgg 
      aattgcagg aaccgtgaa catttttca cggactagg 
      tttgagact actggcctt aggacacca tacaccttt 
      tatgcacat tggaccagc tcacaacta ccgtaactc 
      aagcatggc caagcaagc tgttcgaga tgctggata 
      tgaaatagc acttggttt gatgccaaa atcaatcgg 
      accgttaac tgtggtgtt cactggatc aggaaacgt 
      gtctcaggg ctcccaaga agtgttcca agaactacc 
      acatgaata cgtagctcc cgagtcttt gaaaaaagc 
      ggcagaaca tggcagttt aaataaagt ctatgcttg 
      tgaggtgag gagaagaca ctacttgga acgtgcaga 
      aggtggtgg ttttgaccc agtcgagta tgaggagca 
      tccagaacg ttacatttc cacatttag taaaaaaat 
      tgcaccgta tgcttccat catcattaa cggaattta 
      ctgggctgt taacagtcc caaactttt gactattcc 
      ggacgctaa acatttgtt aaggcctgc acacacgcc 
      ttggctgcc tacttcaat aggtgcgcc agctttgcc 
      tcatcgaat gttggcgat ttgtgatat ttcggctga 
      tcctggagg ttcgattga gtttatgaa tgagtgtac 
      gacgattga tacgccgtt ttgtctcta cgatgctga 
      taggaataa agatacgaa gagttttaa cgggcctgg 
      ggttctggt ctgcagtat tgataatat gccgacgca 
      aattccaag ggaaagtac tgacttttt tggggattt 
      gttgttccc gtatgtgca agatattat aaaatctaa 
      tgcgcagca accgctcga gagtcatag tttttgtcc 
      agctgttga tggggcaat catcgccag caacggcaa 
      actcactcc caactacga atacatcca agaattgcg 
      aaaatcagc aattaaaag caggcacaa agcaagtag 
      cgagtcctc aagcgtgga aaaacaggt cgttatcgt 
      gggcgcagg acgggtagc tgcaccttt ggtggaata 
      cctccacag ggacaaatc cgtcggtat caccgtcgc 
      gtgcgaaca gaaagactt gagcgataa cttagctcg 
      agctttccc cggcatcga aagcctcta cttgaacgc 
      tgtggaaaa ctccaatac tttggaaga aattgtcaa 
      aaaagcaga tgttgctgt ttcaatcct tccagctaa 
      cttgcacca cattgtggc ccaggcatg tatcaagga 
      aggcaccca tatggtcac ggctagtta catgagcca 
      ggaaatgaa gaatttgca ccgagaagc agtcgatgc 
      cggtatcac agtgcttaa tgaagtagg tttggaccc 
      aggaatcga tcatttgtt ggctctaga atgcatcca 
      agaagtgca acaagctgg aggacgaat cacctcatt 
      tgagtcgtt ctgtggagg gctcccggc accagaatt 
      cagcgataa tcctttgag gtacaagtt ttcgtggtc 
      accaagagg cgctctttt gaacacgtt atcgtcagc 
      ccggtattt gagcaaggg ccaaattgt tgaaattag 
      cgcaggagg agagttgat gaatacaac caaaacgct 
      tgattttct ccctggttt caaccttga aggtttccc 
      caatcggga cagcacaat ttatggaaa atactacgg 
      aattgaaga cgctttaac tgttttgag aggcaccat 
      cagatacac gggttttgc ccaagctgc aagaatgtt 
      gcaattttt ggggctgct agacccaga accacatcc 
      agcactgca tgctcaagg gcccgaaat cacctggag 
      aatgctcat ttgcaacct tttgggact cgaaaacca 
      caacatctt ctacgataa cctcaagaa ccaaattgt 
      tgaacggac aggttccga gttttccgt cgatttgct 
      tgaagaatt aggtttgtt ggacgaaca tggtgtgat 
      taaatgtgg atctccatt ggatactct cactcatta 
      cctctcaac taaattggc gttggaaaa acatgagcg 
      tgatttggt gattttgcg tcacgagat cggtattaa 
      ttggccaga caacaggaa agagatcag aggtgttaa 
      ttttgttgt ttatggaga tgctaatgg ctattctgc 
      catggcaaa gactgttgg gtatccagc ggctattgc 
      gacgaaaat gattctgga tggtgaaat acaagatag 
      gggaatgat tcttccatt tgcccccga aatttacag 
      aacgatttt gtccagatt gcgaaatga gggcttgaa 
      tagcactga gacatcgaa atttattta aattattct 
      cgataagac ttttttatt taataaatt gctttggca 
      aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY