Status
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Gene information
General information
TC031688
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002306,TC002307
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 615747..670708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031688
    iBeetle screen iB_00353

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031688 RA
      MGVKSRFCS EWLFRIFAF LAIGWVPEV WSQLVDTEF 
      IPQVSATCK AGHMNIRVA FNNSFSGAV HARDYRTPA 
      CMVHGDGGK VVTLDINLL AQQGASDYC GLLVNNKTE 
      ERSVPIAVR IHRTLELAD DKFYVITCG KAGFRNARN 
      ETSLVSLRL LDGSRKITQ AVYSKPYTL RAEISRPDG 
      TYGFRVKSC FAFNKLNSS VPLIDDKGC PTNGDVIGP 
      FVYDEKKGT ADAQLRSMF RFPESSEVH FQCDIGLCR 
      GSCPEPVCN DGAQSRALT TEEGILMAA TSVFVLDPG 
      DPPLVQELC DEGGVHPTW LLWLCIALG VLFLIMLII 
      NIFLCSAMT CACARTEII EKEPSIIED YDPYRSWHG 
      SQYGSRYSL NGAPQNPKG YTSGGSTMN STRSVSSHS 
      DHYAIVHSR PGSRYKHRG PPSHVGSHY SGK
    CDS sequence
    • >TC031688 RA
      atgggtgtc aaaagtaga ttttgttcc gagtggttg 
      ttccggatt tttgcgttt ttggcgata ggatgggtt 
      ccggaggtt tggtctcaa ctagtggac accgaattc 
      atccctcaa gtgtcagcc acatgcaaa gccggccac 
      atgaacatc cgtgtagct ttcaacaac agtttttcc 
      ggtgctgtc cacgctagg gattaccga acaccggct 
      tgtatggtc catggagac ggtggtaaa gttgtcact 
      ctcgacatc aatctcttg gcccagcaa ggggccagt 
      gattattgt ggactgctc gtcaacaac aaaaccgag 
      gagcgttcc gtccccatc gccgtccgc atccaccgc 
      accctcgag ctcgccgac gacaaattc tacgtgatc 
      acctgcggc aaagccggc ttccgcaac gcccgaaac 
      gaaacttcc ctcgtctcg ttgcgcctc ctggacggc 
      tctcgcaaa atcacccaa gccgtctac agcaagccc 
      tacactctc cgcgccgag atctcgcgc cctgacggc 
      acctacggc ttccgcgtc aagtcctgc ttcgccttc 
      aacaagctc aacagcagc gtgcccctc atcgacgac 
      aagggctgc cccaccaac ggggacgtg atcggccct 
      ttcgtctac gacgagaag aagggcact gcggacgcc 
      caactcaga tcaatgttc cgcttcccg gaaagctcc 
      gaggtgcat ttccagtgc gacatcggc ctgtgtcgg 
      ggctcctgc ccggagccg gtctgcaat gacggggcg 
      cagtcgagg gcgctgacc accgaggag gggattctt 
      atggccgcc accagtgtg tttgtgctg gatccgggc 
      gatccgcca ctggtgcag gagttgtgc gacgaaggg 
      ggtgtccat ccgacgtgg ttgctgtgg ctttgtatt 
      gccctaggg gtgctgttt cttattatg ttgatcatc 
      aatatcttc ttgtgctcg gcaatgacg tgcgcttgt 
      gccaggacg gagattatt gagaaggag ccgagtatt 
      atcgaggat tatgatcct tataggtcc tggcatggg 
      agccagtac ggatcgagg tactcgtta aacggggct 
      ccgcagaac cccaaaggc tacacttct ggtggttcg 
      acgatgaat tcaaccaga tcagtgtcg tcccacagc 
      gaccactac gctatagtc cactcgcga cctggcagt 
      cgctacaaa cacaggggg ccgccttcg cacgtcggc 
      agccactac agtggaaaa taa
    mRNA sequence
    • >TC031688 RA
      ttcaggagc agttatttt cggcgcccg tttaattct 
      aaaatttca gcaaaactt gaaaaatcg tttttccct 
      ctttaattc caatttttc agtcaatgg ttcgtcgta 
      gaggtgaat atgttccgc ccggaaatg aattttgct 
      attgtggtg gtaaagcat ggtcgtttg caccgtatc 
      gaaactact ttccggaga tcccaacgc tagcacaga 
      cgagcaact cttaacttt cattccagt taaattaac 
      acaagagaa tggtgcaaa cgagtgtgt tgtagtaaa 
      acagtggcc gtttttgaa catgtgatt gtttgttta 
      cgtcgaaat gggtgtcaa aagtagatt ttgttccga 
      gtggttgtt ccggatttt tgcgttttt ggcgatagg 
      atgggttcc ggaggtttg gtctcaact agtggacac 
      cgaattcat ccctcaagt gtcagccac atgcaaagc 
      cggccacat gaacatccg tgtagcttt caacaacag 
      tttttccgg tgctgtcca cgctaggga ttaccgaac 
      accggcttg tatggtcca tggagacgg tggtaaagt 
      tgtcactct cgacatcaa tctcttggc ccagcaagg 
      ggccagtga ttattgtgg actgctcgt caacaacaa 
      aaccgagga gcgttccgt ccccatcgc cgtccgcat 
      ccaccgcac cctcgagct cgccgacga caaattcta 
      cgtgatcac ctgcggcaa agccggctt ccgcaacgc 
      ccgaaacga aacttccct cgtctcgtt gcgcctcct 
      ggacggctc tcgcaaaat cacccaagc cgtctacag 
      caagcccta cactctccg cgccgagat ctcgcgccc 
      tgacggcac ctacggctt ccgcgtcaa gtcctgctt 
      cgccttcaa caagctcaa cagcagcgt gcccctcat 
      cgacgacaa gggctgccc caccaacgg ggacgtgat 
      cggcccttt cgtctacga cgagaagaa gggcactgc 
      ggacgccca actcagatc aatgttccg cttcccgga 
      aagctccga ggtgcattt ccagtgcga catcggcct 
      gtgtcgggg ctcctgccc ggagccggt ctgcaatga 
      cggggcgca gtcgagggc gctgaccac cgaggaggg 
      gattcttat ggccgccac cagtgtgtt tgtgctgga 
      tccgggcga tccgccact ggtgcagga gttgtgcga 
      cgaaggggg tgtccatcc gacgtggtt gctgtggct 
      ttgtattgc cctaggggt gctgtttct tattatgtt 
      gatcatcaa tatcttctt gtgctcggc aatgacgtg 
      cgcttgtgc caggacgga gattattga gaaggagcc 
      gagtattat cgaggatta tgatcctta taggtcctg 
      gcatgggag ccagtacgg atcgaggta ctcgttaaa 
      cggggctcc gcagaaccc caaaggcta cacttctgg 
      tggttcgac gatgaattc aaccagatc agtgtcgtc 
      ccacagcga ccactacgc tatagtcca ctcgcgacc 
      tggcagtcg ctacaaaca cagggggcc gccttcgca 
      cgtcggcag ccactacag tggaaaata atattgtgt 
      tcacctaca aactttcga agtgtagca taaggtcca 
      tattaagtg caattacca tattacgat aaacgatat 
      ttatacgac ttactttac tgaaaaacc gtccaggaa 
      aaatatcca gtttaacaa tagactaag cttacgatt 
      cagttaact gtagcttta attttgtaa attctaaaa 
      gtggaaatt gtaaagaga actgattaa aacttattg 
      taaatataa aaattattt tgaaataaa gctttcgtg 
      ttatttagc gccgaaaga gccaacatt tcaagtatt 
      tgtcaagtt gtttcacgt tttaacgaa gcgaaaaaa 
      tgatgaaac aagctttca cctcacgtt tgtttgttg 
      acactgtat tctttattg tagataaga acagattga 
      aacatcaaa agaaagttg ctgggtgta atccgagtt 
      aatacacta aaagtagga ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY