Status
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Gene information
General information
TC031679
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002270
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 304609..309722
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031679
    iBeetle screen iB_00349

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031679 RA
      MPQCAVMGC NSSHRKTKG GSIRYHRFP GDAVTRARW 
      VQACGKYVQ NCSTARICS RHFTDDSYE RDVQHELLG 
      LPTRCRLKK GATPERNLP TDFLKEDTL PDSAIAILL 
      AVGLVPAKA ARQATGGLP QDLTKMQIM EDVREGGSV 
      QPSSPGHAD ERVNEESNK TSPEPEENG FEEEGDNHV 
      EELPKKEMD SEELKDEKP TTEIEIGDN LKSETNLDA 
      NNINEESCS NTSSEEAPN KRKAESQDS ANKRLRLDI 
      QENFISRDK ILNEFIELS DSNTVEQLQ MQTEQILAE 
      IRTLNELAK EKEREWNNI IHLKKMKEE LLLRMQRRK 
      QVMLLSSDR NENGDVYSE SQTDSERLK SANQSILKA 
      NLTNPNKTL RMQNKHRNI LPKHQYSQE MNGSLDFRQ 
      NKQRPVLDV QSIIADYRQ RHPEIVPRR GRRIRNSHN 
      DNKNNILNF SNLALGSGA QVHQNVDLQ SELGILLNA 
      MDGNRGDSR PSSAESSTT HEPSFKDML VQFAKLSQS 
      ERHELIQNA IKPPPPYPE VTVHPVPTT NTVAPTNSL 
      LHGILTKSP SKANTKTSF SPTLARLLT APERSNSHS 
      PTAPTLPNN LNNAANMSI SEILSTSKA RNEITITPL 
      GSQYDSPQK GKSQEDEEA EDSADRLVI DESSEVIDN 
      RGRGDNSSD AGDEVPQCQ GCNQKSALF VCAGCGNQW 
      YCSRDCQVS AWDEHSEVC SG
    CDS sequence
    • >TC031679 RA
      atgccccag tgcgcggtg atgggatgc aacagctcc 
      catcgaaaa acgaaaggc ggctccata cgctaccac 
      cgcttccca ggggacgcc gtgacccgc gctcggtgg 
      gtgcaagcg tgtggaaag tacgtacag aactgctcc 
      acggcgcgg atctgctcc aggcatttc actgacgat 
      agctacgaa agagatgtt cagcacgag ttgctagga 
      ttgcctacg cgctgtcgc ctcaaaaag ggagcaact 
      cctgagagg aacctcccc acggacttc ctcaaggag 
      gacacactt cccgacagc gctatagct atcctgctg 
      gccgtgggg ctggtgccc gctaaggct gcaaggcag 
      gccacgggc ggcttgccc caagatctg accaaaatg 
      cagataatg gaggacgtg cgggaaggt ggcagcgtc 
      cagccctcc tcgccagga catgcggat gaacgcgtc 
      aacgaagaa agcaacaag acgagccca gagcccgaa 
      gaaaacggg ttcgaagaa gaaggcgac aatcacgtt 
      gaagaatta ccaaagaaa gaaatggac agtgaagaa 
      ctgaaagat gaaaagccc acaaccgaa atagaaatc 
      ggcgacaac ctcaaatca gagaccaac ctcgacgcc 
      aacaacatc aacgaggag agttgtagc aacacaagt 
      tcggaggaa gcgccaaac aaacgcaaa gccgagagc 
      caagactcc gccaacaaa agactgcga ctagacatc 
      caagaaaac ttcatctca cgcgacaag atcctcaac 
      gagttcatc gaattgtcc gacagcaac acagtggag 
      cagctccag atgcaaacc gaacagatt ttggccgaa 
      attcggact ctgaatgag ctggccaaa gaaaaggag 
      cgcgaatgg aacaacatc atccacttg aagaaaatg 
      aaggaagag cttttgctg cgcatgcaa cgccggaaa 
      caagtgatg ttgcttagt agtgacagg aatgagaat 
      ggtgatgtt tatagtgaa tctcaaaca gacagtgag 
      cgactaaaa agtgcaaac caaagtatc ctaaaggcg 
      aatctaacg aacccaaac aagacgctc cgaatgcaa 
      aacaagcac cggaacata ctaccaaag catcaatat 
      tcgcaagaa atgaacggg tcgcttgat ttcagacag 
      aataagcaa cggccggtc ctcgatgtt cagtccatc 
      attgccgat taccgacaa agacaccca gaaattgtg 
      cctcggaga ggtcgcaga atccgcaat tctcacaat 
      gacaacaaa aataatatt ctcaacttt tccaacttg 
      gcgctgggt tccggcgct caagtccac caaaacgta 
      gatttgcag agcgaattg ggaatcctg ctgaacgcc 
      atggacggg aatcgtggc gactcacgg ccatcgagt 
      gcggagtct tccactacg catgagccg tctttcaaa 
      gacatgctg gtgcagttt gccaaattg tcccaaagc 
      gaaaggcac gaattgatt cagaatgca atcaaaccg 
      ccgccaccc tatccagag gtgacggtt catccagtt 
      ccgacaact aacaccgtc gcacctaca aattcactt 
      ctgcacggt attctcaca aagagtccg tcaaaggcc 
      aataccaaa acgtcattt agtccaact ttggctcgc 
      cttctcacc gctccagaa cgatccaat tcacattca 
      cccacagca cctacactt cctaataac ttgaataat 
      gcagcgaat atgtctatc tctgaaata ctttccacg 
      agcaaggct aggaatgag ataactata actcctcta 
      ggttctcaa tacgactca ccacagaaa ggcaagtca 
      caggaggac gaggaagct gaagacagt gcagatcgg 
      ctggtaatt gacgaaagt agcgaagtg attgacaac 
      agaggcaga ggtgacaac agctcagat gcaggagat 
      gaagtacct cagtgtcag ggttgcaat caaaagtcg 
      gctctcttc gtttgtgca ggatgtggt aaccagtgg 
      tactgcagc agagactgt caggtatct gcttgggat 
      gaacactct gaagtttgc tccggttag
    mRNA sequence
    • >TC031679 RA
      agaaccgtc ctgtcctaa tttgtttga acccattac 
      cggcatcct atttcgcaa cctcccagc aaacaatac 
      cttcccatt ttattaaca ttagcaatg aaccactta 
      tcaggtaac caacaagat tacaacagt gacgtaaca 
      aattgcgcc gacccaatt ccttatcaa ctctcctta 
      tttaccaga ccaggtgag agcggaaga gattcggat 
      gcttctcaa ttccatccg gtgcaagaa ccgcgacat 
      tatgcacgt ttccacccc ttaagagaa cctttaggg 
      ccactatgc cccagtgcg cggtgatgg gatgcaaca 
      gctcccatc gaaaaacga aaggcggct ccatacgct 
      accaccgct tcccagggg acgccgtga cccgcgctc 
      ggtgggtgc aagcgtgtg gaaagtacg tacagaact 
      gctccacgg cgcggatct gctccaggc atttcactg 
      acgatagct acgaaagag atgttcagc acgagttgc 
      taggattgc ctacgcgct gtcgcctca aaaagggag 
      caactcctg agaggaacc tccccacgg acttcctca 
      aggaggaca cacttcccg acagcgcta tagctatcc 
      tgctggccg tggggctgg tgcccgcta aggctgcaa 
      ggcaggcca cgggcggct tgccccaag atctgacca 
      aaatgcaga taatggagg acgtgcggg aaggtggca 
      gcgtccagc cctcctcgc caggacatg cggatgaac 
      gcgtcaacg aagaaagca acaagacga gcccagagc 
      ccgaagaaa acgggttcg aagaagaag gcgacaatc 
      acgttgaag aattaccaa agaaagaaa tggacagtg 
      aagaactga aagatgaaa agcccacaa ccgaaatag 
      aaatcggcg acaacctca aatcagaga ccaacctcg 
      acgccaaca acatcaacg aggagagtt gtagcaaca 
      caagttcgg aggaagcgc caaacaaac gcaaagccg 
      agagccaag actccgcca acaaaagac tgcgactag 
      acatccaag aaaacttca tctcacgcg acaagatcc 
      tcaacgagt tcatcgaat tgtccgaca gcaacacag 
      tggagcagc tccagatgc aaaccgaac agattttgg 
      ccgaaattc ggactctga atgagctgg ccaaagaaa 
      aggagcgcg aatggaaca acatcatcc acttgaaga 
      aaatgaagg aagagcttt tgctgcgca tgcaacgcc 
      ggaaacaag tgatgttgc ttagtagtg acaggaatg 
      agaatggtg atgtttata gtgaatctc aaacagaca 
      gtgagcgac taaaaagtg caaaccaaa gtatcctaa 
      aggcgaatc taacgaacc caaacaaga cgctccgaa 
      tgcaaaaca agcaccgga acatactac caaagcatc 
      aatattcgc aagaaatga acgggtcgc ttgatttca 
      gacagaata agcaacggc cggtcctcg atgttcagt 
      ccatcattg ccgattacc gacaaagac acccagaaa 
      ttgtgcctc ggagaggtc gcagaatcc gcaattctc 
      acaatgaca acaaaaata atattctca acttttcca 
      acttggcgc tgggttccg gcgctcaag tccaccaaa 
      acgtagatt tgcagagcg aattgggaa tcctgctga 
      acgccatgg acgggaatc gtggcgact cacggccat 
      cgagtgcgg agtcttcca ctacgcatg agccgtctt 
      tcaaagaca tgctggtgc agtttgcca aattgtccc 
      aaagcgaaa ggcacgaat tgattcaga atgcaatca 
      aaccgccgc caccctatc cagaggtga cggttcatc 
      cagttccga caactaaca ccgtcgcac ctacaaatt 
      cacttctgc acggtattc tcacaaaga gtccgtcaa 
      aggccaata ccaaaacgt catttagtc caactttgg 
      ctcgccttc tcaccgctc cagaacgat ccaattcac 
      attcaccca cagcaccta cacttccta ataacttga 
      ataatgcag cgaatatgt ctatctctg aaatacttt 
      ccacgagca aggctagga atgagataa ctataactc 
      ctctaggtt ctcaatacg actcaccac agaaaggca 
      agtcacagg aggacgagg aagctgaag acagtgcag 
      atcggctgg taattgacg aaagtagcg aagtgattg 
      acaacagag gcagaggtg acaacagct cagatgcag 
      gagatgaag tacctcagt gtcagggtt gcaatcaaa 
      agtcggctc tcttcgttt gtgcaggat gtggtaacc 
      agtggtact gcagcagag actgtcagg tatctgctt 
      gggatgaac actctgaag tttgctccg gttagaaat 
      ctgtactta tttttatac tgcatgatg aggttattt 
      attctttaa gaattaaaa tttacacca aataaattt 
      ggtacctgt atagaaaaa tgatgcaat gaaatagca 
      ttccttgga gaagaaaaa ttcttgcct tgaattcgt 
      agtactgta gctattccg ttatgcatc aatgtggct 
      tgattttat ttatagtag aaccaaaga tacccaaag 
      cttaaatga tcatttaat gcattttgc tacaagtag 
      cgagtcaaa tggcattta aatcaaatt tgaatcatc 
      cctatttaa agaaatgtt attttttac aagacgaaa 
      tgtacgtat ttattacaa aatgccaaa acatttcag 
      tgattcaaa aacacaata attataata tgtactaat 
      tagtcaaga agtttattt taaaaaatc acaaccaaa 
      caagtctct tttgttacc tataagaag tagattatc 
      ttcctattt atgaatttg tactagtca gttaagttg 
      tagttaccc aatcagttt gcaatgttc gttttatgt 
      gttgtgtaa gtgccattt atgtaaggg ttgtgtaat 
      tttttatgt ttctatcat ttaggttta agcaggatt 
      ctgtaagat aaatactgt gatatccaa gaatggatc 
      aacgatact taatttaaa ttaaagcca gtatctttc 
      a
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