| General information | |||
| TC002265 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 500833..524795 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002265 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00348 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC002265 RA
MSTKQRVRQ LEACSREMF GDMVLHHQT PPPQQSLFG EVIQAVPVT LTMRKTSME TPSNKYTDY EKRRASDLV EELLVDIYS NINGSDYNS ATSSKSHRS FVADINNLH GKDATELSS LISYLEYHV ERAGSVLIR QLKRRDALR RQQDAHSDV ITGQLLKRS GPDSPEMRF SIQPGPGES GFSQWLAAM KMVAQLSGG IPPEFRKRL WLTLADRHL AARGIDWPN VERNCFSEW SHPTDSELG VQIVKDLHR TGCSLFCGK DGKENQALL KRVLLAYAR WNKAVGYCQ GFNMLAALI LQVTDKCET DALKLMIYL IEGVLPDSY FADSLRGLS VDMAVFREL LRARLPRLS KHLDALQNA AKEGTTSYE PPLTNVFTM QWFLTLFCN CLPQPTVLR VWDLILLEG NEILLRTAL AIWQTLADR IMGVRSADE FYCIMGALT RELLEFELI DANSLIKTV VAIGPLNEL KSLREHYLY NINPWGSSV PTTIFPDSI DKQMKLYAK DRLVLDISA LKKQYAKLK QRQRQAHII FSAAISRQP PPSAPVAMN HLLLGKSAL VPAKRLGPP KVSIPPART TPPSTLLWK DAPKTTSDS SSSSDTELC DDPSEGSSD EQDEEIKSQ FVEDNPENL MNQDNKAAA EEVEANSSN SQITSDIFD SSASTSMKD IFDVVAESD DDSVDFEKF LEDRVRCLK TPTNEEPEE DDRIKYGRR NSERALQII QENSLILHR IMQCQSRLS PPPPFVDQD NATEATIAS PGDKEATSM AFYDTLSSI NLDSSPTRE PKQETTPEY GSRYSSILE KSRNLDKKY NNLILNASV MNLRTVSDS GLADPIIPS VVNNEESKN EKIKNFSFN FDEASSCYN PTSVKQCLK SPCSDNSDK SPSSKSPNK IFNPFPIPL NSRQNKEVP LKLGLYKK
- >TC002265 RA
atgtcaacg aaacagcgt gttcgtcag ttggaagcg tgcagtcgc gaaatgttc ggcgatatg gtcctccac caccaaact ccaccacct caacaatcg ctcttcgga gaagtcatc caagcggta cctgttaca cttacgatg cgcaaaact tcgatggaa acaccgtca aacaaatac acggactac gagaaacga cgagcttcc gatttagtg gaagagctt ttggtcgac atttacagc aacatcaac gggtctgat tacaactct gcaacttcg agcaagtcc catcgcagt ttcgtggct gatattaat aatttgcac gggaaagat gccacagaa ttatcatct cttattagt tatttggaa taccacgtg gagcgcgct gggagtgtt ctgatacgg caactgaag cgccgcgac gctttgcgt cgtcaacaa gacgcacac tctgacgtc ataaccggc caacttcta aaacgaagt ggtccggac agcccagag atgcgtttt tcaatccaa ccaggtcct ggcgaatca ggattttct caatggttg gcagctatg aagatggtg gctcaattg tccggtggc atacctcca gaatttcga aaaagatta tggctcaca ttggctgat agacacttg gcagctcgt ggcatcgat tggcctaat gtcgaaaga aattgcttc agtgagtgg tcgcatcca accgattcg gagttggga gttcaaatt gtaaaggat ttgcaccga actggttgt agccttttt tgcggaaaa gatggaaag gaaaaccag gccttgttg aagagagtg cttctggct tatgccaga tggaacaaa gctgtcggt tattgtcaa ggttttaac atgttagca gctttaata cttcaagtg actgataaa tgtgagact gacgctcta aaactcatg atttatctc atagagggg gtgctcccc gactcatat ttcgcggat agtttgcgt ggactttcc gtcgacatg gcagttttc agggagtta cttcgggcc cgattacca cgtctttca aaacacttg gatgctcta caaaatgcg gccaaagaa ggaacgacc agttacgaa cctcccttg accaacgtt ttcaccatg caatggttt ctaacccta ttttgcaac tgtctgcca caaccaaca gttttgcgc gtttgggat ttgatcctt ctcgaaggt aacgagata cttcttcgc acggcttta gcaatctgg caaactttg gcggatcga ataatgggt gttcgaagc gcggacgag ttctactgc attatgggt gctttaact cgagaactg ctcgagttt gagctaatt gatgcaaat tctctaatt aaaacagta gttgcgata ggtcctctt aacgaactg aagagtctc cgcgaacat tatttgtac aacattaac ccgtggggt agttctgtt ccgacaact atctttccg gatagtata gacaagcag atgaaactg tatgccaaa gacagactg gtgttggat ataagcgcc ctgaagaag caatatgcc aaattgaag cagagacaa agacaggcc cacattatt tttagtgct gctatctcc agacaacct ccgccaagt gctccagta gccatgaac cacttacta ctcgggaag agtgctctg gtcccggct aaaaggtta ggaccgcct aaggtaagt atacctcca gctagaaca actccccct agcaccctc ttgtggaag gatgctcca aaaacaacc agcgatagt tcttcgtca tctgacaca gaactgtgc gatgatcca agcgaaggt tcttcggac gagcaagat gaagaaatc aaaagtcaa ttcgttgaa gacaatccc gaaaatctt atgaatcaa gacaataaa gccgctgct gaagaagtc gaagccaat tcttccaat agtcaaatt actagtgat atttttgat agttccgct tcaactagc atgaaagac atattcgac gtcgtagca gaatcggac gatgacagc gtcgacttt gaaaagttt ctcgaagat cgtgtccga tgccttaaa actccaaca aatgaggag ccggaagag gacgacagg attaaatac gggagaaga aacagcgaa cgcgcctta caaataatt caagagaat tcgttaatt ctccacaga ataatgcaa tgccagtcg agattatca ccaccaccg ccctttgtc gatcaggac aatgcaacc gaagcaaca atagcatcg ccaggagac aaagaagcg acatcaatg gctttctac gacacgtta agcagtatt aacctcgat tcttcgcca actcgggaa ccaaaacag gaaaccact cctgaatac ggctccaga tatagcagc atacttgag aagagtcgg aacctggac aaaaagtac aacaacttg attttaaat gccagtgtt atgaatttg agaactgtg agtgattca ggacttgct gacccaatt attccaagt gttgtgaat aacgaagaa tcaaaaaat gagaaaatc aaaaatttc tcgttcaat tttgatgag gcttcgtct tgctataac cccaccagt gtgaaacaa tgtttaaag tccccgtgc tctgataat agtgataaa tcgccaagt tcaaagtca ccaaataaa atatttaac ccgttcccc attcctttg aattcgcgg caaaacaag gaagttcct ttgaaactg ggattgtac aaaaaatga
- >TC002265 RA
tgtcataca attttctta aattctgtg cggtcgtca gtaagtata gtaattgtt gaagtgcaa catgtcaac gaaacagcg tgttcgtca gttggaagc gtgcagtcg cgaaatgtt cggcgatat ggtcctcca ccaccaaac tccaccacc tcaacaatc gctcttcgg agaagtcat ccaagcggt acctgttac acttacgat gcgcaaaac ttcgatgga aacaccgtc aaacaaata cacggacta cgagaaacg acgagcttc cgatttagt ggaagagct tttggtcga catttacag caacatcaa cgggtctga ttacaactc tgcaacttc gagcaagtc ccatcgcag tttcgtggc tgatattaa taatttgca cgggaaaga tgccacaga attatcatc tcttattag ttatttgga ataccacgt ggagcgcgc tgggagtgt tctgatacg gcaactgaa gcgccgcga cgctttgcg tcgtcaaca agacgcaca ctctgacgt cataaccgg ccaacttct aaaacgaag tggtccgga cagcccaga gatgcgttt ttcaatcca accaggtcc tggcgaatc aggattttc tcaatggtt ggcagctat gaagatggt ggctcaatt gtccggtgg catacctcc agaatttcg aaaaagatt atggctcac attggctga tagacactt ggcagctcg tggcatcga ttggcctaa tgtcgaaag aaattgctt cagtgagtg gtcgcatcc aaccgattc ggagttggg agttcaaat tgtaaagga tttgcaccg aactggttg tagcctttt ttgcggaaa agatggaaa ggaaaacca ggccttgtt gaagagagt gcttctggc ttatgccag atggaacaa agctgtcgg ttattgtca aggttttaa catgttagc agctttaat acttcaagt gactgataa atgtgagac tgacgctct aaaactcat gatttatct catagaggg ggtgctccc cgactcata tttcgcgga tagtttgcg tggactttc cgtcgacat ggcagtttt cagggagtt acttcgggc ccgattacc acgtctttc aaaacactt ggatgctct acaaaatgc ggccaaaga aggaacgac cagttacga acctccctt gaccaacgt tttcaccat gcaatggtt tctaaccct attttgcaa ctgtctgcc acaaccaac agttttgcg cgtttggga tttgatcct tctcgaagg taacgagat acttcttcg cacggcttt agcaatctg gcaaacttt ggcggatcg aataatggg tgttcgaag cgcggacga gttctactg cattatggg tgctttaac tcgagaact gctcgagtt tgagctaat tgatgcaaa ttctctaat taaaacagt agttgcgat aggtcctct taacgaact gaagagtct ccgcgaaca ttatttgta caacattaa cccgtgggg tagttctgt tccgacaac tatctttcc ggatagtat agacaagca gatgaaact gtatgccaa agacagact ggtgttgga tataagcgc cctgaagaa gcaatatgc caaattgaa gcagagaca aagacaggc ccacattat ttttagtgc tgctatctc cagacaacc tccgccaag tgctccagt agccatgaa ccacttact actcgggaa gagtgctct ggtcccggc taaaaggtt aggaccgcc taaggtaag tatacctcc agctagaac aactccccc tagcaccct cttgtggaa ggatgctcc aaaaacaac cagcgatag ttcttcgtc atctgacac agaactgtg cgatgatcc aagcgaagg ttcttcgga cgagcaaga tgaagaaat caaaagtca attcgttga agacaatcc cgaaaatct tatgaatca agacaataa agccgctgc tgaagaagt cgaagccaa ttcttccaa tagtcaaat tactagtga tatttttga tagttccgc ttcaactag catgaaaga catattcga cgtcgtagc agaatcgga cgatgacag cgtcgactt tgaaaagtt tctcgaaga tcgtgtccg atgccttaa aactccaac aaatgagga gccggaaga ggacgacag gattaaata cgggagaag aaacagcga acgcgcctt acaaataat tcaagagaa ttcgttaat tctccacag aataatgca atgccagtc gagattatc accaccacc gccctttgt cgatcagga caatgcaac cgaagcaac aatagcatc gccaggaga caaagaagc gacatcaat ggctttcta cgacacgtt aagcagtat taacctcga ttcttcgcc aactcggga accaaaaca ggaaaccac tcctgaata cggctccag atatagcag catacttga gaagagtcg gaacctgga caaaaagta caacaactt gattttaaa tgccagtgt tatgaattt gagaactgt gagtgattc aggacttgc tgacccaat tattccaag tgttgtgaa taacgaaga atcaaaaaa tgagaaaat caaaaattt ctcgttcaa ttttgatga ggcttcgtc ttgctataa ccccaccag tgtgaaaca atgtttaaa gtccccgtg ctctgataa tagtgataa atcgccaag ttcaaagtc accaaataa aatatttaa cccgttccc cattccttt gaattcgcg gcaaaacaa ggaagttcc tttgaaact gggattgta caaaaaatg acaaaacaa ctttgtatt gtttttatt attgttgtg cttataata aggaaaaga ctgcactca aatttgttg ttattatga acaaagttg taaaaattt aatcgcatt aacactata cttgtatga accatctag atttgatgg cgtgacaaa catttatta ttaataaaa ccttgttga tactatttt gcgctgtgc cggatccgt tctaaagat tttagcaaa taatatatt tccttaaaa attgttata gcgttgact caataaata cattcttgt tta
