Status
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Gene information
General information
TC002265 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 500833..524795
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002265
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00348

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002265 RA
      MSTKQRVRQ LEACSREMF GDMVLHHQT PPPQQSLFG 
      EVIQAVPVT LTMRKTSME TPSNKYTDY EKRRASDLV 
      EELLVDIYS NINGSDYNS ATSSKSHRS FVADINNLH 
      GKDATELSS LISYLEYHV ERAGSVLIR QLKRRDALR 
      RQQDAHSDV ITGQLLKRS GPDSPEMRF SIQPGPGES 
      GFSQWLAAM KMVAQLSGG IPPEFRKRL WLTLADRHL 
      AARGIDWPN VERNCFSEW SHPTDSELG VQIVKDLHR 
      TGCSLFCGK DGKENQALL KRVLLAYAR WNKAVGYCQ 
      GFNMLAALI LQVTDKCET DALKLMIYL IEGVLPDSY 
      FADSLRGLS VDMAVFREL LRARLPRLS KHLDALQNA 
      AKEGTTSYE PPLTNVFTM QWFLTLFCN CLPQPTVLR 
      VWDLILLEG NEILLRTAL AIWQTLADR IMGVRSADE 
      FYCIMGALT RELLEFELI DANSLIKTV VAIGPLNEL 
      KSLREHYLY NINPWGSSV PTTIFPDSI DKQMKLYAK 
      DRLVLDISA LKKQYAKLK QRQRQAHII FSAAISRQP 
      PPSAPVAMN HLLLGKSAL VPAKRLGPP KVSIPPART 
      TPPSTLLWK DAPKTTSDS SSSSDTELC DDPSEGSSD 
      EQDEEIKSQ FVEDNPENL MNQDNKAAA EEVEANSSN 
      SQITSDIFD SSASTSMKD IFDVVAESD DDSVDFEKF 
      LEDRVRCLK TPTNEEPEE DDRIKYGRR NSERALQII 
      QENSLILHR IMQCQSRLS PPPPFVDQD NATEATIAS 
      PGDKEATSM AFYDTLSSI NLDSSPTRE PKQETTPEY 
      GSRYSSILE KSRNLDKKY NNLILNASV MNLRTVSDS 
      GLADPIIPS VVNNEESKN EKIKNFSFN FDEASSCYN 
      PTSVKQCLK SPCSDNSDK SPSSKSPNK IFNPFPIPL 
      NSRQNKEVP LKLGLYKK
    CDS sequence
    • >TC002265 RA
      atgtcaacg aaacagcgt gttcgtcag ttggaagcg 
      tgcagtcgc gaaatgttc ggcgatatg gtcctccac 
      caccaaact ccaccacct caacaatcg ctcttcgga 
      gaagtcatc caagcggta cctgttaca cttacgatg 
      cgcaaaact tcgatggaa acaccgtca aacaaatac 
      acggactac gagaaacga cgagcttcc gatttagtg 
      gaagagctt ttggtcgac atttacagc aacatcaac 
      gggtctgat tacaactct gcaacttcg agcaagtcc 
      catcgcagt ttcgtggct gatattaat aatttgcac 
      gggaaagat gccacagaa ttatcatct cttattagt 
      tatttggaa taccacgtg gagcgcgct gggagtgtt 
      ctgatacgg caactgaag cgccgcgac gctttgcgt 
      cgtcaacaa gacgcacac tctgacgtc ataaccggc 
      caacttcta aaacgaagt ggtccggac agcccagag 
      atgcgtttt tcaatccaa ccaggtcct ggcgaatca 
      ggattttct caatggttg gcagctatg aagatggtg 
      gctcaattg tccggtggc atacctcca gaatttcga 
      aaaagatta tggctcaca ttggctgat agacacttg 
      gcagctcgt ggcatcgat tggcctaat gtcgaaaga 
      aattgcttc agtgagtgg tcgcatcca accgattcg 
      gagttggga gttcaaatt gtaaaggat ttgcaccga 
      actggttgt agccttttt tgcggaaaa gatggaaag 
      gaaaaccag gccttgttg aagagagtg cttctggct 
      tatgccaga tggaacaaa gctgtcggt tattgtcaa 
      ggttttaac atgttagca gctttaata cttcaagtg 
      actgataaa tgtgagact gacgctcta aaactcatg 
      atttatctc atagagggg gtgctcccc gactcatat 
      ttcgcggat agtttgcgt ggactttcc gtcgacatg 
      gcagttttc agggagtta cttcgggcc cgattacca 
      cgtctttca aaacacttg gatgctcta caaaatgcg 
      gccaaagaa ggaacgacc agttacgaa cctcccttg 
      accaacgtt ttcaccatg caatggttt ctaacccta 
      ttttgcaac tgtctgcca caaccaaca gttttgcgc 
      gtttgggat ttgatcctt ctcgaaggt aacgagata 
      cttcttcgc acggcttta gcaatctgg caaactttg 
      gcggatcga ataatgggt gttcgaagc gcggacgag 
      ttctactgc attatgggt gctttaact cgagaactg 
      ctcgagttt gagctaatt gatgcaaat tctctaatt 
      aaaacagta gttgcgata ggtcctctt aacgaactg 
      aagagtctc cgcgaacat tatttgtac aacattaac 
      ccgtggggt agttctgtt ccgacaact atctttccg 
      gatagtata gacaagcag atgaaactg tatgccaaa 
      gacagactg gtgttggat ataagcgcc ctgaagaag 
      caatatgcc aaattgaag cagagacaa agacaggcc 
      cacattatt tttagtgct gctatctcc agacaacct 
      ccgccaagt gctccagta gccatgaac cacttacta 
      ctcgggaag agtgctctg gtcccggct aaaaggtta 
      ggaccgcct aaggtaagt atacctcca gctagaaca 
      actccccct agcaccctc ttgtggaag gatgctcca 
      aaaacaacc agcgatagt tcttcgtca tctgacaca 
      gaactgtgc gatgatcca agcgaaggt tcttcggac 
      gagcaagat gaagaaatc aaaagtcaa ttcgttgaa 
      gacaatccc gaaaatctt atgaatcaa gacaataaa 
      gccgctgct gaagaagtc gaagccaat tcttccaat 
      agtcaaatt actagtgat atttttgat agttccgct 
      tcaactagc atgaaagac atattcgac gtcgtagca 
      gaatcggac gatgacagc gtcgacttt gaaaagttt 
      ctcgaagat cgtgtccga tgccttaaa actccaaca 
      aatgaggag ccggaagag gacgacagg attaaatac 
      gggagaaga aacagcgaa cgcgcctta caaataatt 
      caagagaat tcgttaatt ctccacaga ataatgcaa 
      tgccagtcg agattatca ccaccaccg ccctttgtc 
      gatcaggac aatgcaacc gaagcaaca atagcatcg 
      ccaggagac aaagaagcg acatcaatg gctttctac 
      gacacgtta agcagtatt aacctcgat tcttcgcca 
      actcgggaa ccaaaacag gaaaccact cctgaatac 
      ggctccaga tatagcagc atacttgag aagagtcgg 
      aacctggac aaaaagtac aacaacttg attttaaat 
      gccagtgtt atgaatttg agaactgtg agtgattca 
      ggacttgct gacccaatt attccaagt gttgtgaat 
      aacgaagaa tcaaaaaat gagaaaatc aaaaatttc 
      tcgttcaat tttgatgag gcttcgtct tgctataac 
      cccaccagt gtgaaacaa tgtttaaag tccccgtgc 
      tctgataat agtgataaa tcgccaagt tcaaagtca 
      ccaaataaa atatttaac ccgttcccc attcctttg 
      aattcgcgg caaaacaag gaagttcct ttgaaactg 
      ggattgtac aaaaaatga
    mRNA sequence
    • >TC002265 RA
      tgtcataca attttctta aattctgtg cggtcgtca 
      gtaagtata gtaattgtt gaagtgcaa catgtcaac 
      gaaacagcg tgttcgtca gttggaagc gtgcagtcg 
      cgaaatgtt cggcgatat ggtcctcca ccaccaaac 
      tccaccacc tcaacaatc gctcttcgg agaagtcat 
      ccaagcggt acctgttac acttacgat gcgcaaaac 
      ttcgatgga aacaccgtc aaacaaata cacggacta 
      cgagaaacg acgagcttc cgatttagt ggaagagct 
      tttggtcga catttacag caacatcaa cgggtctga 
      ttacaactc tgcaacttc gagcaagtc ccatcgcag 
      tttcgtggc tgatattaa taatttgca cgggaaaga 
      tgccacaga attatcatc tcttattag ttatttgga 
      ataccacgt ggagcgcgc tgggagtgt tctgatacg 
      gcaactgaa gcgccgcga cgctttgcg tcgtcaaca 
      agacgcaca ctctgacgt cataaccgg ccaacttct 
      aaaacgaag tggtccgga cagcccaga gatgcgttt 
      ttcaatcca accaggtcc tggcgaatc aggattttc 
      tcaatggtt ggcagctat gaagatggt ggctcaatt 
      gtccggtgg catacctcc agaatttcg aaaaagatt 
      atggctcac attggctga tagacactt ggcagctcg 
      tggcatcga ttggcctaa tgtcgaaag aaattgctt 
      cagtgagtg gtcgcatcc aaccgattc ggagttggg 
      agttcaaat tgtaaagga tttgcaccg aactggttg 
      tagcctttt ttgcggaaa agatggaaa ggaaaacca 
      ggccttgtt gaagagagt gcttctggc ttatgccag 
      atggaacaa agctgtcgg ttattgtca aggttttaa 
      catgttagc agctttaat acttcaagt gactgataa 
      atgtgagac tgacgctct aaaactcat gatttatct 
      catagaggg ggtgctccc cgactcata tttcgcgga 
      tagtttgcg tggactttc cgtcgacat ggcagtttt 
      cagggagtt acttcgggc ccgattacc acgtctttc 
      aaaacactt ggatgctct acaaaatgc ggccaaaga 
      aggaacgac cagttacga acctccctt gaccaacgt 
      tttcaccat gcaatggtt tctaaccct attttgcaa 
      ctgtctgcc acaaccaac agttttgcg cgtttggga 
      tttgatcct tctcgaagg taacgagat acttcttcg 
      cacggcttt agcaatctg gcaaacttt ggcggatcg 
      aataatggg tgttcgaag cgcggacga gttctactg 
      cattatggg tgctttaac tcgagaact gctcgagtt 
      tgagctaat tgatgcaaa ttctctaat taaaacagt 
      agttgcgat aggtcctct taacgaact gaagagtct 
      ccgcgaaca ttatttgta caacattaa cccgtgggg 
      tagttctgt tccgacaac tatctttcc ggatagtat 
      agacaagca gatgaaact gtatgccaa agacagact 
      ggtgttgga tataagcgc cctgaagaa gcaatatgc 
      caaattgaa gcagagaca aagacaggc ccacattat 
      ttttagtgc tgctatctc cagacaacc tccgccaag 
      tgctccagt agccatgaa ccacttact actcgggaa 
      gagtgctct ggtcccggc taaaaggtt aggaccgcc 
      taaggtaag tatacctcc agctagaac aactccccc 
      tagcaccct cttgtggaa ggatgctcc aaaaacaac 
      cagcgatag ttcttcgtc atctgacac agaactgtg 
      cgatgatcc aagcgaagg ttcttcgga cgagcaaga 
      tgaagaaat caaaagtca attcgttga agacaatcc 
      cgaaaatct tatgaatca agacaataa agccgctgc 
      tgaagaagt cgaagccaa ttcttccaa tagtcaaat 
      tactagtga tatttttga tagttccgc ttcaactag 
      catgaaaga catattcga cgtcgtagc agaatcgga 
      cgatgacag cgtcgactt tgaaaagtt tctcgaaga 
      tcgtgtccg atgccttaa aactccaac aaatgagga 
      gccggaaga ggacgacag gattaaata cgggagaag 
      aaacagcga acgcgcctt acaaataat tcaagagaa 
      ttcgttaat tctccacag aataatgca atgccagtc 
      gagattatc accaccacc gccctttgt cgatcagga 
      caatgcaac cgaagcaac aatagcatc gccaggaga 
      caaagaagc gacatcaat ggctttcta cgacacgtt 
      aagcagtat taacctcga ttcttcgcc aactcggga 
      accaaaaca ggaaaccac tcctgaata cggctccag 
      atatagcag catacttga gaagagtcg gaacctgga 
      caaaaagta caacaactt gattttaaa tgccagtgt 
      tatgaattt gagaactgt gagtgattc aggacttgc 
      tgacccaat tattccaag tgttgtgaa taacgaaga 
      atcaaaaaa tgagaaaat caaaaattt ctcgttcaa 
      ttttgatga ggcttcgtc ttgctataa ccccaccag 
      tgtgaaaca atgtttaaa gtccccgtg ctctgataa 
      tagtgataa atcgccaag ttcaaagtc accaaataa 
      aatatttaa cccgttccc cattccttt gaattcgcg 
      gcaaaacaa ggaagttcc tttgaaact gggattgta 
      caaaaaatg acaaaacaa ctttgtatt gtttttatt 
      attgttgtg cttataata aggaaaaga ctgcactca 
      aatttgttg ttattatga acaaagttg taaaaattt 
      aatcgcatt aacactata cttgtatga accatctag 
      atttgatgg cgtgacaaa catttatta ttaataaaa 
      ccttgttga tactatttt gcgctgtgc cggatccgt 
      tctaaagat tttagcaaa taatatatt tccttaaaa 
      attgttata gcgttgact caataaata cattcttgt 
      tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY