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Gene information
General information
TC031691
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002251,TC002252
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 1131344..1150325
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031691
    iBeetle screen iB_00347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031691 RA
      MVYPHSYRT EHSETRTTN SVYGEYYAQ HQSKRIPIQ 
      PDLLKIIQF IDSHRGRFL KDLADVVMI KSVSGNLEY 
      KDEVQKMID FTQNWLSKL GLKYERFNI GFHELGGEK 
      HRLPVILLA SLGNDQRKK TLCIYVHLD VKEPEASKW 
      QTDPWSVSQ VGHSIFGCG VAQGKATLI HWFHIIEAF 
      QKSNIEFPV NLKFIIESM YHYDSVGLA DFLPLRKHD 
      FFVDIDNII VCDGEWIGE KHPCIIYGT IGVIHHDIF 
      IEKVPGSKT DPRDDMVEI FKEIVDEKE HILIPHFDD 
      IVSQITPEE EKMYEDICD FNIDDIRDN LPSYKQKWD 
      KVKLLMHYW RLPSISIGE TEECTCEKK EVDIVKRHF 
      VVKIVPRQI INRTVNMTK NHINSVVKK LNIENKVTC 
      EEVSSTRYW HESVANPNY QAARRATIQ IYKEDPSMI 
      KLDKPMVLL TILDRVLEN NILLLPLVT SGSNASMEN 
      ENITVRTFY ESTKLLAAY LFQLAK
    CDS sequence
    • >TC031691 RA
      atggtgtac cctcactct tacagaact gaacacagc 
      gaaacgcga actacaaac agtgtctac ggagagtat 
      tacgcccaa catcagagt aaaagaatt ccaattcaa 
      cccgatttg ttaaaaatc atccaattt attgactca 
      caccgtggc aggtttctc aaagatttg gccgatgtt 
      gtgatgatc aaatcagta tccggcaat ttggaatac 
      aaagatgag gtgcaaaaa atgattgat tttacccaa 
      aactggctc tcgaaactt ggactaaag tatgagcgt 
      ttcaatatt gggtttcat gaactcggg ggtgagaaa 
      caccgacta ccggtcatt cttttggcc tctttagga 
      aacgatcaa cgaaagaaa acgctttgc atttatgtg 
      catttggac gttaaggag ccagaggcg agtaaatgg 
      cagacagat ccgtggagt gtcagtcaa gttggtcat 
      tcgattttt ggctgtggc gtagcgcag ggaaaagct 
      actcttatt cattggttc cacattatt gaagcgttt 
      cagaaaagt aatattgaa ttcccggtt aatttgaaa 
      tttatcatt gagtcgatg taccactac gatagtgtc 
      ggtttggcc gattttttg cctttgaga aaacacgat 
      ttttttgtc gatattgat aatatcatt gtttgtgat 
      ggtgagtgg attggtgag aagcaccct tgcatcatt 
      tatggtact attggggtc atacatcat gatattttt 
      attgaaaaa gttcctggc tctaaaact gatccgagg 
      gacgatatg gttgaaata tttaaggaa attgttgac 
      gagaaggaa catattttg atcccgcac tttgatgat 
      attgtctcg caaattact ccagaggag gagaagatg 
      tatgaagat atttgtgat ttcaacatt gatgacata 
      agagacaat ttgccctct tacaagcaa aaatgggat 
      aaagtcaaa ctcctaatg cattattgg cgcctacct 
      tcgatatca attggtgaa accgaagaa tgcacttgt 
      gagaagaaa gaagtggac attgttaaa agacatttt 
      gttgtaaaa atcgttcct cgccaaatt atcaacaga 
      acagtgaat atgacaaaa aatcacatt aacagtgtt 
      gttaagaaa cttaacatt gaaaataaa gtcacttgc 
      gaggaagtt tcttcaacc agatattgg cacgaaagt 
      gtagccaat cccaattat caagctgca agaagggca 
      acaatccaa atttataaa gaagatccg agcatgatc 
      aaacttgat aaacctatg gttttgtta actattctt 
      gacagagtt ctggagaat aatattctt cttctgcca 
      ttggttacg tctggttct aacgcaagc atggaaaac 
      gaaaatata acagtacga acgttttat gagagtacc 
      aagcttctg gctgcttat ttgtttcaa ttagcaaaa 
      tga
    mRNA sequence
    • >TC031691 RA
      gaggccaaa aagatattt gtctaccga tatatcact 
      ggtggtggt ggcgctttg tacgttgtg ctgacgaaa 
      ttggaagaa agactactt catgtactg ggaaatgtt 
      aatgtaaat ggttttaaa aatgtaatc gagttgggt 
      ttatgtgaa aggaaaaca aaagtatgt tttagaact 
      attgcaact aagattgat acatttttt tagctaaag 
      aaaaagata tctattgag attgggtac aaatgcaaa 
      caacgcaac cgccataag tatcgtctt acaaaaaaa 
      acaaaaaga agctgaaaa aatgtattt taactactc 
      caaatgttc aacaaatgc tacttttaa aattcttaa 
      cgccgtcaa acatgtgaa ccaatctct tcaacgttt 
      aataaatat gttttctta aaaatattg atttattat 
      tctttccct tattctctg ttctgcatt gaagaaatg 
      attggtaaa aaatggtgt accctcact cttacagaa 
      ctgaacaca gcgaaacgc gaactacaa acagtgtct 
      acggagagt attacgccc aacatcaga gtaaaagaa 
      ttccaattc aacccgatt tgttaaaaa tcatccaat 
      ttattgact cacaccgtg gcaggtttc tcaaagatt 
      tggccgatg ttgtgatga tcaaatcag tatccggca 
      atttggaat acaaagatg aggtgcaaa aaatgattg 
      attttaccc aaaactggc tctcgaaac ttggactaa 
      agtatgagc gtttcaata ttgggtttc atgaactcg 
      ggggtgaga aacaccgac taccggtca ttcttttgg 
      cctctttag gaaacgatc aacgaaaga aaacgcttt 
      gcatttatg tgcatttgg acgttaagg agccagagg 
      cgagtaaat ggcagacag atccgtgga gtgtcagtc 
      aagttggtc attcgattt ttggctgtg gcgtagcgc 
      agggaaaag ctactctta ttcattggt tccacatta 
      ttgaagcgt ttcagaaaa gtaatattg aattcccgg 
      ttaatttga aatttatca ttgagtcga tgtaccact 
      acgatagtg tcggtttgg ccgattttt tgcctttga 
      gaaaacacg atttttttg tcgatattg ataatatca 
      ttgtttgtg atggtgagt ggattggtg agaagcacc 
      cttgcatca tttatggta ctattgggg tcatacatc 
      atgatattt ttattgaaa aagttcctg gctctaaaa 
      ctgatccga gggacgata tggttgaaa tatttaagg 
      aaattgttg acgagaagg aacatattt tgatcccgc 
      actttgatg atattgtct cgcaaatta ctccagagg 
      aggagaaga tgtatgaag atatttgtg atttcaaca 
      ttgatgaca taagagaca atttgccct cttacaagc 
      aaaaatggg ataaagtca aactcctaa tgcattatt 
      ggcgcctac cttcgatat caattggtg aaaccgaag 
      aatgcactt gtgagaaga aagaagtgg acattgtta 
      aaagacatt ttgttgtaa aaatcgttc ctcgccaaa 
      ttatcaaca gaacagtga atatgacaa aaaatcaca 
      ttaacagtg ttgttaaga aacttaaca ttgaaaata 
      aagtcactt gcgaggaag tttcttcaa ccagatatt 
      ggcacgaaa gtgtagcca atcccaatt atcaagctg 
      caagaaggg caacaatcc aaatttata aagaagatc 
      cgagcatga tcaaacttg ataaaccta tggttttgt 
      taactattc ttgacagag ttctggaga ataatattc 
      ttcttctgc cattggtta cgtctggtt ctaacgcaa 
      gcatggaaa acgaaaata taacagtac gaacgtttt 
      atgagagta ccaagcttc tggctgctt atttgtttc 
      aattagcaa aatgatgat aaatacaga gtgattacg 
      caaaatcgt ttttttttt caacttcag taaaaaaat 
      aaaaagtac taatttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY