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Gene information
General information
TC031691
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002251,TC002252 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451937.1: 1131344..1150325
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031691
    iBeetle screen iB_00347

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031691 RA
      MVYPHSYRT EHSETRTTN SVYGEYYAQ HQSKRIPIQ 
PDLLKIIQF IDSHRGRFL KDLADVVMI KSVSGNLEY 
KDEVQKMID FTQNWLSKL GLKYERFNI GFHELGGEK 
HRLPVILLA SLGNDQRKK TLCIYVHLD VKEPEASKW 
QTDPWSVSQ VGHSIFGCG VAQGKATLI HWFHIIEAF 
QKSNIEFPV NLKFIIESM YHYDSVGLA DFLPLRKHD 
FFVDIDNII VCDGEWIGE KHPCIIYGT IGVIHHDIF 
IEKVPGSKT DPRDDMVEI FKEIVDEKE HILIPHFDD 
IVSQITPEE EKMYEDICD FNIDDIRDN LPSYKQKWD 
KVKLLMHYW RLPSISIGE TEECTCEKK EVDIVKRHF 
VVKIVPRQI INRTVNMTK NHINSVVKK LNIENKVTC 
EEVSSTRYW HESVANPNY QAARRATIQ IYKEDPSMI 
KLDKPMVLL TILDRVLEN NILLLPLVT SGSNASMEN 
ENITVRTFY ESTKLLAAY LFQLAK
    CDS sequence
    • >TC031691 RA
      atggtgtac cctcactct tacagaact gaacacagc 
gaaacgcga actacaaac agtgtctac ggagagtat 
tacgcccaa catcagagt aaaagaatt ccaattcaa 
cccgatttg ttaaaaatc atccaattt attgactca 
caccgtggc aggtttctc aaagatttg gccgatgtt 
gtgatgatc aaatcagta tccggcaat ttggaatac 
aaagatgag gtgcaaaaa atgattgat tttacccaa 
aactggctc tcgaaactt ggactaaag tatgagcgt 
ttcaatatt gggtttcat gaactcggg ggtgagaaa 
caccgacta ccggtcatt cttttggcc tctttagga 
aacgatcaa cgaaagaaa acgctttgc atttatgtg 
catttggac gttaaggag ccagaggcg agtaaatgg 
cagacagat ccgtggagt gtcagtcaa gttggtcat 
tcgattttt ggctgtggc gtagcgcag ggaaaagct 
actcttatt cattggttc cacattatt gaagcgttt 
cagaaaagt aatattgaa ttcccggtt aatttgaaa 
tttatcatt gagtcgatg taccactac gatagtgtc 
ggtttggcc gattttttg cctttgaga aaacacgat 
ttttttgtc gatattgat aatatcatt gtttgtgat 
ggtgagtgg attggtgag aagcaccct tgcatcatt 
tatggtact attggggtc atacatcat gatattttt 
attgaaaaa gttcctggc tctaaaact gatccgagg 
gacgatatg gttgaaata tttaaggaa attgttgac 
gagaaggaa catattttg atcccgcac tttgatgat 
attgtctcg caaattact ccagaggag gagaagatg 
tatgaagat atttgtgat ttcaacatt gatgacata 
agagacaat ttgccctct tacaagcaa aaatgggat 
aaagtcaaa ctcctaatg cattattgg cgcctacct 
tcgatatca attggtgaa accgaagaa tgcacttgt 
gagaagaaa gaagtggac attgttaaa agacatttt 
gttgtaaaa atcgttcct cgccaaatt atcaacaga 
acagtgaat atgacaaaa aatcacatt aacagtgtt 
gttaagaaa cttaacatt gaaaataaa gtcacttgc 
gaggaagtt tcttcaacc agatattgg cacgaaagt 
gtagccaat cccaattat caagctgca agaagggca 
acaatccaa atttataaa gaagatccg agcatgatc 
aaacttgat aaacctatg gttttgtta actattctt 
gacagagtt ctggagaat aatattctt cttctgcca 
ttggttacg tctggttct aacgcaagc atggaaaac 
gaaaatata acagtacga acgttttat gagagtacc 
aagcttctg gctgcttat ttgtttcaa ttagcaaaa 
tga
    mRNA sequence
    • >TC031691 RA
      gaggccaaa aagatattt gtctaccga tatatcact 
ggtggtggt ggcgctttg tacgttgtg ctgacgaaa 
ttggaagaa agactactt catgtactg ggaaatgtt 
aatgtaaat ggttttaaa aatgtaatc gagttgggt 
ttatgtgaa aggaaaaca aaagtatgt tttagaact 
attgcaact aagattgat acatttttt tagctaaag 
aaaaagata tctattgag attgggtac aaatgcaaa 
caacgcaac cgccataag tatcgtctt acaaaaaaa 
acaaaaaga agctgaaaa aatgtattt taactactc 
caaatgttc aacaaatgc tacttttaa aattcttaa 
cgccgtcaa acatgtgaa ccaatctct tcaacgttt 
aataaatat gttttctta aaaatattg atttattat 
tctttccct tattctctg ttctgcatt gaagaaatg 
attggtaaa aaatggtgt accctcact cttacagaa 
ctgaacaca gcgaaacgc gaactacaa acagtgtct 
acggagagt attacgccc aacatcaga gtaaaagaa 
ttccaattc aacccgatt tgttaaaaa tcatccaat 
ttattgact cacaccgtg gcaggtttc tcaaagatt 
tggccgatg ttgtgatga tcaaatcag tatccggca 
atttggaat acaaagatg aggtgcaaa aaatgattg 
attttaccc aaaactggc tctcgaaac ttggactaa 
agtatgagc gtttcaata ttgggtttc atgaactcg 
ggggtgaga aacaccgac taccggtca ttcttttgg 
cctctttag gaaacgatc aacgaaaga aaacgcttt 
gcatttatg tgcatttgg acgttaagg agccagagg 
cgagtaaat ggcagacag atccgtgga gtgtcagtc 
aagttggtc attcgattt ttggctgtg gcgtagcgc 
agggaaaag ctactctta ttcattggt tccacatta 
ttgaagcgt ttcagaaaa gtaatattg aattcccgg 
ttaatttga aatttatca ttgagtcga tgtaccact 
acgatagtg tcggtttgg ccgattttt tgcctttga 
gaaaacacg atttttttg tcgatattg ataatatca 
ttgtttgtg atggtgagt ggattggtg agaagcacc 
cttgcatca tttatggta ctattgggg tcatacatc 
atgatattt ttattgaaa aagttcctg gctctaaaa 
ctgatccga gggacgata tggttgaaa tatttaagg 
aaattgttg acgagaagg aacatattt tgatcccgc 
actttgatg atattgtct cgcaaatta ctccagagg 
aggagaaga tgtatgaag atatttgtg atttcaaca 
ttgatgaca taagagaca atttgccct cttacaagc 
aaaaatggg ataaagtca aactcctaa tgcattatt 
ggcgcctac cttcgatat caattggtg aaaccgaag 
aatgcactt gtgagaaga aagaagtgg acattgtta 
aaagacatt ttgttgtaa aaatcgttc ctcgccaaa 
ttatcaaca gaacagtga atatgacaa aaaatcaca 
ttaacagtg ttgttaaga aacttaaca ttgaaaata 
aagtcactt gcgaggaag tttcttcaa ccagatatt 
ggcacgaaa gtgtagcca atcccaatt atcaagctg 
caagaaggg caacaatcc aaatttata aagaagatc 
cgagcatga tcaaacttg ataaaccta tggttttgt 
taactattc ttgacagag ttctggaga ataatattc 
ttcttctgc cattggtta cgtctggtt ctaacgcaa 
gcatggaaa acgaaaata taacagtac gaacgtttt 
atgagagta ccaagcttc tggctgctt atttgtttc 
aattagcaa aatgatgat aaatacaga gtgattacg 
caaaatcgt ttttttttt caacttcag taaaaaaat 
aaaaagtac taatttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY