| General information | |||
| TC031691
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451937.1: 1131344..1150325 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031691 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00347 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
remarks: animals lost,
- >TC031691 RA
MVYPHSYRT EHSETRTTN SVYGEYYAQ HQSKRIPIQ PDLLKIIQF IDSHRGRFL KDLADVVMI KSVSGNLEY KDEVQKMID FTQNWLSKL GLKYERFNI GFHELGGEK HRLPVILLA SLGNDQRKK TLCIYVHLD VKEPEASKW QTDPWSVSQ VGHSIFGCG VAQGKATLI HWFHIIEAF QKSNIEFPV NLKFIIESM YHYDSVGLA DFLPLRKHD FFVDIDNII VCDGEWIGE KHPCIIYGT IGVIHHDIF IEKVPGSKT DPRDDMVEI FKEIVDEKE HILIPHFDD IVSQITPEE EKMYEDICD FNIDDIRDN LPSYKQKWD KVKLLMHYW RLPSISIGE TEECTCEKK EVDIVKRHF VVKIVPRQI INRTVNMTK NHINSVVKK LNIENKVTC EEVSSTRYW HESVANPNY QAARRATIQ IYKEDPSMI KLDKPMVLL TILDRVLEN NILLLPLVT SGSNASMEN ENITVRTFY ESTKLLAAY LFQLAK
- >TC031691 RA
atggtgtac cctcactct tacagaact gaacacagc gaaacgcga actacaaac agtgtctac ggagagtat tacgcccaa catcagagt aaaagaatt ccaattcaa cccgatttg ttaaaaatc atccaattt attgactca caccgtggc aggtttctc aaagatttg gccgatgtt gtgatgatc aaatcagta tccggcaat ttggaatac aaagatgag gtgcaaaaa atgattgat tttacccaa aactggctc tcgaaactt ggactaaag tatgagcgt ttcaatatt gggtttcat gaactcggg ggtgagaaa caccgacta ccggtcatt cttttggcc tctttagga aacgatcaa cgaaagaaa acgctttgc atttatgtg catttggac gttaaggag ccagaggcg agtaaatgg cagacagat ccgtggagt gtcagtcaa gttggtcat tcgattttt ggctgtggc gtagcgcag ggaaaagct actcttatt cattggttc cacattatt gaagcgttt cagaaaagt aatattgaa ttcccggtt aatttgaaa tttatcatt gagtcgatg taccactac gatagtgtc ggtttggcc gattttttg cctttgaga aaacacgat ttttttgtc gatattgat aatatcatt gtttgtgat ggtgagtgg attggtgag aagcaccct tgcatcatt tatggtact attggggtc atacatcat gatattttt attgaaaaa gttcctggc tctaaaact gatccgagg gacgatatg gttgaaata tttaaggaa attgttgac gagaaggaa catattttg atcccgcac tttgatgat attgtctcg caaattact ccagaggag gagaagatg tatgaagat atttgtgat ttcaacatt gatgacata agagacaat ttgccctct tacaagcaa aaatgggat aaagtcaaa ctcctaatg cattattgg cgcctacct tcgatatca attggtgaa accgaagaa tgcacttgt gagaagaaa gaagtggac attgttaaa agacatttt gttgtaaaa atcgttcct cgccaaatt atcaacaga acagtgaat atgacaaaa aatcacatt aacagtgtt gttaagaaa cttaacatt gaaaataaa gtcacttgc gaggaagtt tcttcaacc agatattgg cacgaaagt gtagccaat cccaattat caagctgca agaagggca acaatccaa atttataaa gaagatccg agcatgatc aaacttgat aaacctatg gttttgtta actattctt gacagagtt ctggagaat aatattctt cttctgcca ttggttacg tctggttct aacgcaagc atggaaaac gaaaatata acagtacga acgttttat gagagtacc aagcttctg gctgcttat ttgtttcaa ttagcaaaa tga
- >TC031691 RA
gaggccaaa aagatattt gtctaccga tatatcact ggtggtggt ggcgctttg tacgttgtg ctgacgaaa ttggaagaa agactactt catgtactg ggaaatgtt aatgtaaat ggttttaaa aatgtaatc gagttgggt ttatgtgaa aggaaaaca aaagtatgt tttagaact attgcaact aagattgat acatttttt tagctaaag aaaaagata tctattgag attgggtac aaatgcaaa caacgcaac cgccataag tatcgtctt acaaaaaaa acaaaaaga agctgaaaa aatgtattt taactactc caaatgttc aacaaatgc tacttttaa aattcttaa cgccgtcaa acatgtgaa ccaatctct tcaacgttt aataaatat gttttctta aaaatattg atttattat tctttccct tattctctg ttctgcatt gaagaaatg attggtaaa aaatggtgt accctcact cttacagaa ctgaacaca gcgaaacgc gaactacaa acagtgtct acggagagt attacgccc aacatcaga gtaaaagaa ttccaattc aacccgatt tgttaaaaa tcatccaat ttattgact cacaccgtg gcaggtttc tcaaagatt tggccgatg ttgtgatga tcaaatcag tatccggca atttggaat acaaagatg aggtgcaaa aaatgattg attttaccc aaaactggc tctcgaaac ttggactaa agtatgagc gtttcaata ttgggtttc atgaactcg ggggtgaga aacaccgac taccggtca ttcttttgg cctctttag gaaacgatc aacgaaaga aaacgcttt gcatttatg tgcatttgg acgttaagg agccagagg cgagtaaat ggcagacag atccgtgga gtgtcagtc aagttggtc attcgattt ttggctgtg gcgtagcgc agggaaaag ctactctta ttcattggt tccacatta ttgaagcgt ttcagaaaa gtaatattg aattcccgg ttaatttga aatttatca ttgagtcga tgtaccact acgatagtg tcggtttgg ccgattttt tgcctttga gaaaacacg atttttttg tcgatattg ataatatca ttgtttgtg atggtgagt ggattggtg agaagcacc cttgcatca tttatggta ctattgggg tcatacatc atgatattt ttattgaaa aagttcctg gctctaaaa ctgatccga gggacgata tggttgaaa tatttaagg aaattgttg acgagaagg aacatattt tgatcccgc actttgatg atattgtct cgcaaatta ctccagagg aggagaaga tgtatgaag atatttgtg atttcaaca ttgatgaca taagagaca atttgccct cttacaagc aaaaatggg ataaagtca aactcctaa tgcattatt ggcgcctac cttcgatat caattggtg aaaccgaag aatgcactt gtgagaaga aagaagtgg acattgtta aaagacatt ttgttgtaa aaatcgttc ctcgccaaa ttatcaaca gaacagtga atatgacaa aaaatcaca ttaacagtg ttgttaaga aacttaaca ttgaaaata aagtcactt gcgaggaag tttcttcaa ccagatatt ggcacgaaa gtgtagcca atcccaatt atcaagctg caagaaggg caacaatcc aaatttata aagaagatc cgagcatga tcaaacttg ataaaccta tggttttgt taactattc ttgacagag ttctggaga ataatattc ttcttctgc cattggtta cgtctggtt ctaacgcaa gcatggaaa acgaaaata taacagtac gaacgtttt atgagagta ccaagcttc tggctgctt atttgtttc aattagcaa aatgatgat aaatacaga gtgattacg caaaatcgt ttttttttt caacttcag taaaaaaat aaaaagtac taatttct
