Status
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Gene information
General information
TC031127
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002244,TC002245
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 672814..681610
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031127
    iBeetle screen iB_00346

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    iBeetle screen iB_06698

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031127 RA
      MSTQKGNAQ RSRSQKHQN KTAFKNTLH 
      DTSQRTKNI NNIQISDVC VRCKEIIEW 
      KIKYKKYKP LTQPRKCVR CDQKNVKKA 
      YHVMCTDCG KKFGVCTKC CETKEVITT 
      KPDEKEQLK LDNEMKNLL HTLPERKRR 
      TFLRYMSKK SEEDSTNDE ERKEDLKSK 
      LNALKIDDD LDDFSDFDD VDSSDD
    • >TC031127 RB
      MIGRLFASK TIFRRVLRP LYTLPKVRN TPKLFLVTS 
      YPIFSKERS KDTMDNFDL HLKAACEKG EVAITQLMN 
      SFRDHILET SKAYKECLQ KQIEIIDEA TKVGAMSAK 
      WDELPEYRS IATDLSQQL NTFATTVHT LSEIAKNET 
      LQYITQQQK QFKLIGKKT KELEKLLTN QMEEIKKLE 
      SKVLAKKKM STQKGNAQR SRSQKHQNK TAFKNTLHD 
      TSQRTKNIN NIQISDVCV RCKEIIEWK IKYKKYKPL 
      TQPRKCVRC DQKNVKKAY HVMCTDCGK KFGVCTKCC 
      ETKEVITTK PDEKEQLKL DNEMKNLLH TLPERKRRT 
      FLRYMSKKS EEDSTNDEE RKEDLKSKL NALKIDDDL 
      DDFSDFDDV DSSDD
    • >TC031127 RC
      MIGRLFASK TIFRRVLRP LYTLPKVRN TPKLFLVTS 
      YPIFSKERS KDTMDNFDL HLKAACEKG EVAITQLMN 
      SFRDHILET SKAYKECLQ KQIEIIDEA TKVGAMSAK 
      WDELPEYRS IATDLSQQL NTFATTVHT LSEIAKNET 
      LQYITQQQK QFKLIGKKT KELEKLLTN QMEEIKKLE 
      SKVLAKSRE SIQKSK
    CDS sequence
    • >TC031127 RA
      atgagcacc caaaaaggt aacgcccaa aggagtcgc 
      tcccaaaaa catcaaaac aaaaccgcc ttcaaaaac 
      accctccac gacacaagt caacgcacg aaaaacatt 
      aacaacatc caaatcagc gacgtttgt gtccgttgc 
      aaggaaata atcgagtgg aaaataaag tacaaaaaa 
      tacaaaccg ctcacacag ccacgaaaa tgtgtcaga 
      tgtgatcag aaaaacgtg aagaaagcg tatcatgtg 
      atgtgtaca gactgtgga aaaaaattt ggtgtttgt 
      acaaaatgt tgtgaaacg aaagaagtt attacaaca 
      aaaccggac gaaaaggaa caactcaaa ctggacaac 
      gaaatgaag aatttgcta catacgctt ccggaacgc 
      aaacggagg acgtttttg aggtacatg agcaaaaaa 
      agtgaggaa gattcgacg aatgacgag gagagaaag 
      gaagatttg aaaagcaaa ctcaacgcg cttaagatt 
      gacgatgac ttggacgac ttcagcgat tttgatgac 
      gttgattcg tcggatgat taa
    • >TC031127 RB
      atgatcggc agattgttc gcttcaaaa accattttc 
      cgaagagtt ttacgacct ctatatact ctgcctaaa 
      gtcagaaac acaccaaag ctctttctt gttacttcc 
      tatccaatt ttctcaaaa gagcgctca aaagacaca 
      atggataat tttgatttg cacttaaag gcagcttgt 
      gagaagggt gaagtagca attacgcaa ctgatgaat 
      tccttccgc gatcatatt ctggaaaca agcaaagct 
      tacaaggaa tgtttacaa aaacaaatc gaaattatt 
      gacgaagcc acaaaagtg ggtgctatg tcggcaaaa 
      tgggacgaa ttgcccgaa tatagaagt atagctact 
      gatttgagc caacagttg aatactttt gctactact 
      gttcatact cttagtgaa attgctaaa aatgaaact 
      ttacaatac atcacgcaa caacagaag caatttaag 
      ttgattggc aagaagacc aaggaactg gaaaaatta 
      ctaacgaac caaatggaa gaaataaag aaactggag 
      tcgaaagtt ttagccaag aaaaaaatg agcacccaa 
      aaaggtaac gcccaaagg agtcgctcc caaaaacat 
      caaaacaaa accgccttc aaaaacacc ctccacgac 
      acaagtcaa cgcacgaaa aacattaac aacatccaa 
      atcagcgac gtttgtgtc cgttgcaag gaaataatc 
      gagtggaaa ataaagtac aaaaaatac aaaccgctc 
      acacagcca cgaaaatgt gtcagatgt gatcagaaa 
      aacgtgaag aaagcgtat catgtgatg tgtacagac 
      tgtggaaaa aaatttggt gtttgtaca aaatgttgt 
      gaaacgaaa gaagttatt acaacaaaa ccggacgaa 
      aaggaacaa ctcaaactg gacaacgaa atgaagaat 
      ttgctacat acgcttccg gaacgcaaa cggaggacg 
      tttttgagg tacatgagc aaaaaaagt gaggaagat 
      tcgacgaat gacgaggag agaaaggaa gatttgaaa 
      agcaaactc aacgcgctt aagattgac gatgacttg 
      gacgacttc agcgatttt gatgacgtt gattcgtcg 
      gatgattaa
    • >TC031127 RC
      atgatcggc agattgttc gcttcaaaa accattttc 
      cgaagagtt ttacgacct ctatatact ctgcctaaa 
      gtcagaaac acaccaaag ctctttctt gttacttcc 
      tatccaatt ttctcaaaa gagcgctca aaagacaca 
      atggataat tttgatttg cacttaaag gcagcttgt 
      gagaagggt gaagtagca attacgcaa ctgatgaat 
      tccttccgc gatcatatt ctggaaaca agcaaagct 
      tacaaggaa tgtttacaa aaacaaatc gaaattatt 
      gacgaagcc acaaaagtg ggtgctatg tcggcaaaa 
      tgggacgaa ttgcccgaa tatagaagt atagctact 
      gatttgagc caacagttg aatactttt gctactact 
      gttcatact cttagtgaa attgctaaa aatgaaact 
      ttacaatac atcacgcaa caacagaag caatttaag 
      ttgattggc aagaagacc aaggaactg gaaaaatta 
      ctaacgaac caaatggaa gaaataaag aaactggag 
      tcgaaagtt ttagccaag agtcgagaa agcatacaa 
      aaatcgaaa taa
    mRNA sequence
    • >TC031127 RA
      ctagatacc aaaaaaacc tgatttgca aaatcaaga 
      gtcaatgca tattttatt tatttagcc ttaaggaac 
      attgtcaac attcacgta agtaataat acaaaaaaa 
      aaactaaaa aaatgcttt ggtcctgac ataaaatat 
      aaataaaaa ataataata tgaaaaatt atttgtctg 
      gtttagtct tgaaacaaa gatacacat tccagaaca 
      acgcaacaa cctgagcat acaaaactt gatactgca 
      atggtacaa aatagaaat taatggttt gagccgctg 
      gccacaact tataattag cgaataaaa tatcgggat 
      atgtttctt ttatcgcat gcttcacct cctcgactc 
      tcttcccct gcaaggccc ctatactaa ccataaacc 
      ccaaaagac acaccggag cgaaacaaa tctgatcga 
      gcgctactt tcgtaatcg caacacgtt tcccgcttt 
      ttcctacat atttatcca aaacgcggt accaaaccg 
      tcaatgcag gtccctgta atgtgattg ttgtttatt 
      agagagaaa aaatgagca cccaaaaag gtaacgccc 
      aaaggagtc gctcccaaa aacatcaaa acaaaaccg 
      ccttcaaaa acaccctcc acgacacaa gtcaacgca 
      cgaaaaaca ttaacaaca tccaaatca gcgacgttt 
      gtgtccgtt gcaaggaaa taatcgagt ggaaaataa 
      agtacaaaa aatacaaac cgctcacac agccacgaa 
      aatgtgtca gatgtgatc agaaaaacg tgaagaaag 
      cgtatcatg tgatgtgta cagactgtg gaaaaaaat 
      ttggtgttt gtacaaaat gttgtgaaa cgaaagaag 
      ttattacaa caaaaccgg acgaaaagg aacaactca 
      aactggaca acgaaatga agaatttgc tacatacgc 
      ttccggaac gcaaacgga ggacgtttt tgaggtaca 
      tgagcaaaa aaagtgagg aagattcga cgaatgacg 
      aggagagaa aggaagatt tgaaaagca aactcaacg 
      cgcttaaga ttgacgatg acttggacg acttcagcg 
      attttgatg acgttgatt cgtcggatg attaaattt 
      tgtaatata ttggttgta gaatttaat taaaataat 
      aaaataaaa gagcgaat
    • >TC031127 RB
      aatgtccta aatttttgc cgataacac ttttaaaca 
      cccgaaaat gatcggcag attgttcgc ttcaaaaac 
      cattttccg aagagtttt acgacctct atatactct 
      gcctaaagt cagaaacac accaaagct ctttcttgt 
      tacttccta tccaatttt ctcaaaaga gcgctcaaa 
      agacacaat ggataattt tgatttgca cttaaaggc 
      agcttgtga gaagggtga agtagcaat tacgcaact 
      gatgaattc cttccgcga tcatattct ggaaacaag 
      caaagctta caaggaatg tttacaaaa acaaatcga 
      aattattga cgaagccac aaaagtggg tgctatgtc 
      ggcaaaatg ggacgaatt gcccgaata tagaagtat 
      agctactga tttgagcca acagttgaa tacttttgc 
      tactactgt tcatactct tagtgaaat tgctaaaaa 
      tgaaacttt acaatacat cacgcaaca acagaagca 
      atttaagtt gattggcaa gaagaccaa ggaactgga 
      aaaattact aacgaacca aatggaaga aataaagaa 
      actggagtc gaaagtttt agccaagaa aaaaatgag 
      cacccaaaa aggtaacgc ccaaaggag tcgctccca 
      aaaacatca aaacaaaac cgccttcaa aaacaccct 
      ccacgacac aagtcaacg cacgaaaaa cattaacaa 
      catccaaat cagcgacgt ttgtgtccg ttgcaagga 
      aataatcga gtggaaaat aaagtacaa aaaatacaa 
      accgctcac acagccacg aaaatgtgt cagatgtga 
      tcagaaaaa cgtgaagaa agcgtatca tgtgatgtg 
      tacagactg tggaaaaaa atttggtgt ttgtacaaa 
      atgttgtga aacgaaaga agttattac aacaaaacc 
      ggacgaaaa ggaacaact caaactgga caacgaaat 
      gaagaattt gctacatac gcttccgga acgcaaacg 
      gaggacgtt tttgaggta catgagcaa aaaaagtga 
      ggaagattc gacgaatga cgaggagag aaaggaaga 
      tttgaaaag caaactcaa cgcgcttaa gattgacga 
      tgacttgga cgacttcag cgattttga tgacgttga 
      ttcgtcgga tgattaaat tttgtaata tattggttg 
      tagaattta attaaaata ataaaataa aagagcgaa 
      t
    • >TC031127 RC
      aaagatcta aatttgctt tgaagaaac gtaacacta 
      ttactggat ttcaggtaa cttcattca taatgtcct 
      aaatttttg ccgataaca cttttaaac acccgaaaa 
      tgatcggca gattgttcg cttcaaaaa ccattttcc 
      gaagagttt tacgacctc tatatactc tgcctaaag 
      tcagaaaca caccaaagc tctttcttg ttacttcct 
      atccaattt tctcaaaag agcgctcaa aagacacaa 
      tggataatt ttgatttgc acttaaagg cagcttgtg 
      agaagggtg aagtagcaa ttacgcaac tgatgaatt 
      ccttccgcg atcatattc tggaaacaa gcaaagctt 
      acaaggaat gtttacaaa aacaaatcg aaattattg 
      acgaagcca caaaagtgg gtgctatgt cggcaaaat 
      gggacgaat tgcccgaat atagaagta tagctactg 
      atttgagcc aacagttga atacttttg ctactactg 
      ttcatactc ttagtgaaa ttgctaaaa atgaaactt 
      tacaataca tcacgcaac aacagaagc aatttaagt 
      tgattggca agaagacca aggaactgg aaaaattac 
      taacgaacc aaatggaag aaataaaga aactggagt 
      cgaaagttt tagccaaga gtcgagaaa gcatacaaa 
      aatcgaaat aaatctaga aaatgttta tttaaccaa 
      taaaaattt gacattatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY