Status
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Gene information
General information
TC002220 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1098132..1102543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002220
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00345

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002220 RA
      MTSADFEKF REKVSKAKH DYCGTFDSN GFRTGSGCE 
      KWLKENTHQ KYTGQFLYN NLHNKGVYL NDEKGNQTY 
      MDGLFFSNK LEGYGRVIC ADNTVFEGL FKQHVRFGP 
      GVVTHLDGS QDVGIWDGF SLIKLCVCV GDDFVPRLG 
      KSSLGKARL LHFRSSVPI CPKPPDPAK QFLDLESDQ 
      LYNVHVKNP RSLVFNNVE YQKSFFTEQ ECTIDELVS 
      EEEFNEDEM FEEIVTKPE CDCFQPNLN RFGHMCVQL 
      QEVESKLEI ITSKKTRLE EKLSQCKYC CDHIVVPTK 
      PKTKHHQIE EETEVVETE ITKTATQKK ESRKVLSFY 
      SCSSTASEN SEYHNITRS EIELKNYLY FTESTASIE 
      EQVEQTAGC PLRDKSSGF TLPFSHWDS DSSDLQFNK 
      PHLINFDVY SKYVEEEEE EVSPKPSPL YHCFCTEKL 
      IENINILKR QLQTIVQEE TFYHTVRNT IKNHLDIEF 
      SRLNQNEER RRFVRKVVV RDVLAWNNE KLWITMLQH 
      CFRHRFSEH NVRFNVCDL LTGQRQYFQ NAGDYEAAC 
      KNFLEECSQ GSEQNVFNY IRTKNINPD LCDARGNTG 
      LMLATLGEK MNCVSMLVD CGAKVDAMN DEGLTPLTM 
      SLLKYISSK FQVTDWEKA FLKVQTSPE LFHTQQWRA 
      TESLLTQLC DSKTMIYST DLSANEQKY IFNTDFIRV 
      FATPKKKFD KKKGREEKC DERQLITIK KTILTLLRF 
      GADPRIGDV PLKPIFLSV FTDDSDILQ GLLKSNADP 
      NSLFSDEHL TCLHLIVSL KPSLEKVKM CEILLSHSA 
      DPNIKTHPN HWSQLRKEL LGEGNDYCD EGKNALHML 
      CLREDFFND SEDLLIWIA SILVANGCR TDDLYLGHT 
      PLSLAVIRG NTKLIESLL KRVDPYQLL GNEMGNALT 
      VLILNRFPS ILPKDVKKD VAKCLIHNG LNPLTRIGE 
      FENVIAFME NEQLLNVKS KKKTTKKKT KKKNKKQLS 
      HSKKGKKKK KSKSSSRGH RKTKTESVE DFIISETRQ 
      TLYRHLQGK AIKFLYTLL CEAIVADPL TVVLAKFVK 
      PHEVLGIIQ LLFQHGAID FSHFSYSLI SDLVQFVRI 
      QHHFNEKQS EEEFNKLKE TIKNTPWKP LKRISRNIY 
      LPPPETDDQ EDKYIVCFQ CLKSEGKHL IRCPSCELV 
      YFCSEHCNR LNDKISKFH PCKVTFYDA VLEIIKDQV 
      QPSRINVLL KMAEKMRKE RIAMIHLLE LEEKVKKLK 
      FIPTRTRIR FEELYNKFQ IAEKEREEF AHRNSLWDY 
      ASILMQNKS QSAVRDVYF EKIPDVAAN RIYKSYSNV 
      FTKVASELS NASSNVDSK FVDTFRSYQ NILYNSPQG 
      ISPTSTAVM FTPKPRRFS QFMLSKNSS STLRIAELQ 
      RKPEEKRKS TLVNGAVKD DLVSQGSKK EKHEERKDK 
      NYYMDLLSK IFADFNLPL LLLPYACYK DGQVYYSMT 
      GNMSYLGSS FHKLPIPD
    CDS sequence
    • >TC002220 RA
      atgacatca gcagatttc gaaaaattt agagaaaaa 
      gtttcaaaa gccaagcac gactattgc ggtacgttc 
      gattcaaac ggatttcga actggctca ggttgtgaa 
      aagtggctg aaagaaaat actcaccaa aaatacacc 
      ggtcagttt ctgtacaac aacttacac aacaaagga 
      gtgtaccta aacgatgag aaaggcaat caaacttac 
      atggatggt ttattcttt tcaaataag ctggaaggt 
      tatgggcgt gtcatatgc gcagacaac acagttttt 
      gaaggttta tttaaacaa catgttcgt tttggtcct 
      ggtgttgtc acacacttg gatggaagt caagatgtg 
      ggaatctgg gacgggttt tcactgata aaactatgt 
      gtttgtgtc ggagatgac tttgtgcct cgtttgggg 
      aaaagttct ttaggcaaa gcgagattg ttacacttt 
      cggtcaagt gtgccgatt tgtcccaag ccaccagat 
      ccagctaaa caattctta gatctggaa tcagatcag 
      ctttacaat gtacacgtc aaaaaccct cgcagtctc 
      gtttttaac aacgttgag taccaaaag agttttttc 
      accgagcaa gaatgcaca attgatgaa ttggtctcc 
      gaagaagaa tttaatgaa gacgaaatg tttgaagaa 
      atagtaaca aagcctgag tgtgattgt tttcaacca 
      aatctgaac aggtttggc cacatgtgt gttcaatta 
      caagaagtg gaatcaaaa ctggaaata attacaagc 
      aagaaaaca cgtcttgag gaaaaactg agccaatgc 
      aaatattgt tgcgaccac attgttgtc cccaccaaa 
      ccaaaaacg aaacatcat caaattgaa gaagaaaca 
      gaagttgtt gaaacagaa attacgaaa acagccaca 
      caaaagaag gagtcccga aaagtgttg tcattttat 
      tcctgctct tccacagct tcggaaaat agtgaatat 
      cacaatatc acacgatct gaaattgaa ttgaaaaat 
      tacttgtac ttcaccgaa tcaacagct tctattgaa 
      gaacaagtt gagcaaaca gctggttgt ccgttacgc 
      gacaaatcc tcaggattt actttacct ttcagtcat 
      tgggacagt gattcgagt gatttgcag ttcaacaaa 
      ccgcacttg attaacttt gatgtgtat tccaagtac 
      gtggaagaa gaggaagaa gaggtttct cctaaacct 
      tcaccactt tatcattgt ttttgcact gaaaagtta 
      atcgaaaac attaacatt ttaaagcgc cagctccaa 
      acaatcgtc caggaagag acgttctat cacacagtt 
      cgaaacact attaagaat cacttagat attgaattt 
      agtagattg aatcagaac gaagagagg agacgattc 
      gttcgtaag gtagtggta agggatgtg ttggcttgg 
      aacaacgaa aaactatgg attacaatg ctccaacat 
      tgttttcgc caccgattt tcggaacat aacgttcgg 
      tttaacgtt tgtgattta ttaacagga cagagacag 
      tatttccaa aacgctgga gattatgaa gcagcttgt 
      aaaaatttc ctggaagaa tgtagccaa gggagcgaa 
      caaaacgtt ttcaattat atcagaacg aagaacata 
      aatcctgat ctttgtgat gctcgggga aacacaggt 
      cttatgttg gccacactg ggggagaaa atgaactgt 
      gtttcgatg ttggttgat tgtggggcc aaagtcgat 
      gcaatgaac gacgaggga ttgactccc ttaacaatg 
      tctctcctt aaatacatt tcgagcaaa ttccaagtg 
      actgactgg gaaaaagcg tttttaaaa gtgcaaact 
      agtccagaa ttgtttcac acacaacaa tggcgcgcc 
      actgaatcg cttctaaca caactttgt gattctaaa 
      acaatgata tattcaaca gatttgagt gccaatgaa 
      cagaagtac attttcaac actgatttt attcgtgtt 
      tttgcgaca ccaaagaaa aaatttgat aagaaaaag 
      ggacgggaa gaaaagtgt gacgaaaga caattgata 
      acaattaag aagactatt ttaacattg ttgcgtttt 
      ggtgccgac cctcgtatt ggtgatgtt cccttaaaa 
      ccaatcttt ttgtcggtg tttactgac gattctgat 
      attttacaa ggtctcctc aaaagtaac gcagaccca 
      aactcgctt ttttccgac gaacatttg acttgtttg 
      catttgatc gtttcgttg aagccttct ttagagaaa 
      gtaaaaatg tgtgaaata ctcctaagt cacagtgct 
      gatcccaat atcaagaca catcccaat cattggtcc 
      cagttgaga aaagaatta cttggagaa ggaaacgat 
      tattgtgat gaaggcaaa aacgcacta catatgttg 
      tgtctacga gaggatttt ttcaacgat tctgaggat 
      ctcctaatt tggattgca agtattttg gtggcgaat 
      ggttgcaga accgatgat ttgtattta ggacatacg 
      cctttatca ctagctgtg attagaggc aatactaaa 
      cttatcgaa tccttgctt aagagagtt gatccttac 
      caattgctg ggaaatgaa atggggaac gcgttaact 
      gtgttgatc ctaaatcgg tttccttca attttgcca 
      aaggatgtt aaaaaggat gtggcgaaa tgtttgata 
      cataacggc ttaaacccc ttgacccga atcggtgaa 
      tttgaaaac gtcattgct ttcatggag aatgaacaa 
      cttttaaat gtgaagtcg aaaaagaaa acaactaag 
      aaaaagacg aagaagaaa aataaaaaa caactctca 
      cattcaaaa aaaggaaag aagaaaaag aaatctaaa 
      agctcgtca cgcggccat cgaaaaact aaaacagaa 
      tcagtggag gatttcata atttcggaa acgaggcag 
      actttgtat cgacacttg caaggcaaa gcaatcaaa 
      ttcttgtat acccttctg tgcgaagct atcgttgcc 
      gatccgcta acagttgtt ttggcaaaa tttgtcaaa 
      cctcacgag gttttaggc ataatccag ctattgttc 
      caacatggt gcaattgac tttagccac ttcagttat 
      tcccttatt agtgatttg gttcagttt gtgaggatc 
      cagcaccat ttcaacgag aaacaaagt gaggaggaa 
      tttaacaaa ttgaaagaa acaattaag aacacaccg 
      tggaaacct ttgaaacgc atctctcgc aatatctat 
      ctcccacca cctgaaact gacgatcag gaggataaa 
      tatattgtt tgcttccag tgtttgaag agtgaggga 
      aagcacttg atcaggtgt ccaagttgt gagttggtt 
      tacttctgt tcggaacat tgtaatcgc ttgaacgac 
      aaaatttcg aaatttcat ccttgcaaa gttacgttt 
      tacgacgca gttttggaa attatcaaa gatcaagtg 
      caaccaagt cgaattaat gtcctgttg aaaatggca 
      gaaaagatg agaaaagag cgaatagcg atgatacat 
      ttgttagaa ttggaagaa aaggtaaag aagttgaaa 
      tttatacca actaggact aggattcga tttgaggaa 
      ctttacaac aagtttcaa attgccgaa aaggaaagg 
      gaagagttt gcacataga aacagttta tgggactat 
      gcaagtatt cttatgcag aataaatcg caaagtgct 
      gtaagggac gtatacttt gagaaaata ccagatgtg 
      gcagcgaat cgaatttac aaaagttac tcaaatgtt 
      ttcaccaaa gtcgcttca gaacttagt aacgcgtcc 
      tcgaatgtt gatagtaaa tttgttgat acgtttcgc 
      tcttaccag aatattctg tacaatagt cctcaaggt 
      atatcacca acttctact gcagtaatg tttacacca 
      aaacctcgt cgtttctcg cagtttatg cttagcaaa 
      aactcatca tcgacttta agaattgcc gaacttcag 
      agaaaacct gaagagaaa aggaagtcc acgcttgtg 
      aatggagca gtgaaggat gatctagtt tcacaaggt 
      agcaaaaaa gagaaacat gaagaaagg aaagataaa 
      aattactat atggacttg ctgtctaaa atttttgcc 
      gattttaat cttcccctg ctgctgtta ccctacgct 
      tgctataaa gatggtcaa gtctattat agtatgacg 
      ggcaacatg tcttacttg gggtccagt ttccataaa 
      ctaccaatt cctgactaa
    mRNA sequence
    • >TC002220 RA
      taagtaaaa tgccaaaat gacatcagc agatttcga 
      aaaatttag agaaaaagt ttcaaaagc caagcacga 
      ctattgcgg tacgttcga ttcaaacgg atttcgaac 
      tggctcagg ttgtgaaaa gtggctgaa agaaaatac 
      tcaccaaaa atacaccgg tcagtttct gtacaacaa 
      cttacacaa caaaggagt gtacctaaa cgatgagaa 
      aggcaatca aacttacat ggatggttt attcttttc 
      aaataagct ggaaggtta tgggcgtgt catatgcgc 
      agacaacac agtttttga aggtttatt taaacaaca 
      tgttcgttt tggtcctgg tgttgtcac acacttgga 
      tggaagtca agatgtggg aatctggga cgggttttc 
      actgataaa actatgtgt ttgtgtcgg agatgactt 
      tgtgcctcg tttggggaa aagttcttt aggcaaagc 
      gagattgtt acactttcg gtcaagtgt gccgatttg 
      tcccaagcc accagatcc agctaaaca attcttaga 
      tctggaatc agatcagct ttacaatgt acacgtcaa 
      aaaccctcg cagtctcgt ttttaacaa cgttgagta 
      ccaaaagag ttttttcac cgagcaaga atgcacaat 
      tgatgaatt ggtctccga agaagaatt taatgaaga 
      cgaaatgtt tgaagaaat agtaacaaa gcctgagtg 
      tgattgttt tcaaccaaa tctgaacag gtttggcca 
      catgtgtgt tcaattaca agaagtgga atcaaaact 
      ggaaataat tacaagcaa gaaaacacg tcttgagga 
      aaaactgag ccaatgcaa atattgttg cgaccacat 
      tgttgtccc caccaaacc aaaaacgaa acatcatca 
      aattgaaga agaaacaga agttgttga aacagaaat 
      tacgaaaac agccacaca aaagaagga gtcccgaaa 
      agtgttgtc attttattc ctgctcttc cacagcttc 
      ggaaaatag tgaatatca caatatcac acgatctga 
      aattgaatt gaaaaatta cttgtactt caccgaatc 
      aacagcttc tattgaaga acaagttga gcaaacagc 
      tggttgtcc gttacgcga caaatcctc aggatttac 
      tttaccttt cagtcattg ggacagtga ttcgagtga 
      tttgcagtt caacaaacc gcacttgat taactttga 
      tgtgtattc caagtacgt ggaagaaga ggaagaaga 
      ggtttctcc taaaccttc accacttta tcattgttt 
      ttgcactga aaagttaat cgaaaacat taacatttt 
      aaagcgcca gctccaaac aatcgtcca ggaagagac 
      gttctatca cacagttcg aaacactat taagaatca 
      cttagatat tgaatttag tagattgaa tcagaacga 
      agagaggag acgattcgt tcgtaaggt agtggtaag 
      ggatgtgtt ggcttggaa caacgaaaa actatggat 
      tacaatgct ccaacattg ttttcgcca ccgattttc 
      ggaacataa cgttcggtt taacgtttg tgatttatt 
      aacaggaca gagacagta tttccaaaa cgctggaga 
      ttatgaagc agcttgtaa aaatttcct ggaagaatg 
      tagccaagg gagcgaaca aaacgtttt caattatat 
      cagaacgaa gaacataaa tcctgatct ttgtgatgc 
      tcggggaaa cacaggtct tatgttggc cacactggg 
      ggagaaaat gaactgtgt ttcgatgtt ggttgattg 
      tggggccaa agtcgatgc aatgaacga cgagggatt 
      gactccctt aacaatgtc tctccttaa atacatttc 
      gagcaaatt ccaagtgac tgactggga aaaagcgtt 
      tttaaaagt gcaaactag tccagaatt gtttcacac 
      acaacaatg gcgcgccac tgaatcgct tctaacaca 
      actttgtga ttctaaaac aatgatata ttcaacaga 
      tttgagtgc caatgaaca gaagtacat tttcaacac 
      tgattttat tcgtgtttt tgcgacacc aaagaaaaa 
      atttgataa gaaaaaggg acgggaaga aaagtgtga 
      cgaaagaca attgataac aattaagaa gactatttt 
      aacattgtt gcgttttgg tgccgaccc tcgtattgg 
      tgatgttcc cttaaaacc aatcttttt gtcggtgtt 
      tactgacga ttctgatat tttacaagg tctcctcaa 
      aagtaacgc agacccaaa ctcgctttt ttccgacga 
      acatttgac ttgtttgca tttgatcgt ttcgttgaa 
      gccttcttt agagaaagt aaaaatgtg tgaaatact 
      cctaagtca cagtgctga tcccaatat caagacaca 
      tcccaatca ttggtccca gttgagaaa agaattact 
      tggagaagg aaacgatta ttgtgatga aggcaaaaa 
      cgcactaca tatgttgtg tctacgaga ggatttttt 
      caacgattc tgaggatct cctaatttg gattgcaag 
      tattttggt ggcgaatgg ttgcagaac cgatgattt 
      gtatttagg acatacgcc tttatcact agctgtgat 
      tagaggcaa tactaaact tatcgaatc cttgcttaa 
      gagagttga tccttacca attgctggg aaatgaaat 
      ggggaacgc gttaactgt gttgatcct aaatcggtt 
      tccttcaat tttgccaaa ggatgttaa aaaggatgt 
      ggcgaaatg tttgataca taacggctt aaacccctt 
      gacccgaat cggtgaatt tgaaaacgt cattgcttt 
      catggagaa tgaacaact tttaaatgt gaagtcgaa 
      aaagaaaac aactaagaa aaagacgaa gaagaaaaa 
      taaaaaaca actctcaca ttcaaaaaa aggaaagaa 
      gaaaaagaa atctaaaag ctcgtcacg cggccatcg 
      aaaaactaa aacagaatc agtggagga tttcataat 
      ttcggaaac gaggcagac tttgtatcg acacttgca 
      aggcaaagc aatcaaatt cttgtatac ccttctgtg 
      cgaagctat cgttgccga tccgctaac agttgtttt 
      ggcaaaatt tgtcaaacc tcacgaggt tttaggcat 
      aatccagct attgttcca acatggtgc aattgactt 
      tagccactt cagttattc ccttattag tgatttggt 
      tcagtttgt gaggatcca gcaccattt caacgagaa 
      acaaagtga ggaggaatt taacaaatt gaaagaaac 
      aattaagaa cacaccgtg gaaaccttt gaaacgcat 
      ctctcgcaa tatctatct cccaccacc tgaaactga 
      cgatcagga ggataaata tattgtttg cttccagtg 
      tttgaagag tgagggaaa gcacttgat caggtgtcc 
      aagttgtga gttggttta cttctgttc ggaacattg 
      taatcgctt gaacgacaa aatttcgaa atttcatcc 
      ttgcaaagt tacgtttta cgacgcagt tttggaaat 
      tatcaaaga tcaagtgca accaagtcg aattaatgt 
      cctgttgaa aatggcaga aaagatgag aaaagagcg 
      aatagcgat gatacattt gttagaatt ggaagaaaa 
      ggtaaagaa gttgaaatt tataccaac taggactag 
      gattcgatt tgaggaact ttacaacaa gtttcaaat 
      tgccgaaaa ggaaaggga agagtttgc acatagaaa 
      cagtttatg ggactatgc aagtattct tatgcagaa 
      taaatcgca aagtgctgt aagggacgt atactttga 
      gaaaatacc agatgtggc agcgaatcg aatttacaa 
      aagttactc aaatgtttt caccaaagt cgcttcaga 
      acttagtaa cgcgtcctc gaatgttga tagtaaatt 
      tgttgatac gtttcgctc ttaccagaa tattctgta 
      caatagtcc tcaaggtat atcaccaac ttctactgc 
      agtaatgtt tacaccaaa acctcgtcg tttctcgca 
      gtttatgct tagcaaaaa ctcatcatc gactttaag 
      aattgccga acttcagag aaaacctga agagaaaag 
      gaagtccac gcttgtgaa tggagcagt gaaggatga 
      tctagtttc acaaggtag caaaaaaga gaaacatga 
      agaaaggaa agataaaaa ttactatat ggacttgct 
      gtctaaaat ttttgccga ttttaatct tcccctgct 
      gctgttacc ctacgcttg ctataaaga tggtcaagt 
      ctattatag tatgacggg caacatgtc ttacttggg 
      gtccagttt ccataaact accaattcc tgactaata 
      aataaatac catctttta aa
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