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Gene information
General information
TC002193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 349079..351013
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002193
    iBeetle screen iB_00344
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    labium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002193 RA
      MALLLIFLF FTPLIITSY EHCQTPNQQ PGKCVIINQ 
      CPHLFSVVL QNATGEESK KLIQKSHCG FESLLFPKV 
      CCPLEAIEL LEESGAISQ NKHSTKCGL HVPDGPSDP 
      WLALLEYEH GDGRREFLC GGVLINKRY VLTAAHCIT 
      QKPKLGTVH LGEYRSTSG LQLVIPVDG TIVHPEYKP 
      NDPHQYHDI ALVRLKQKV NFKDGIVRP ICVPMDSRK 
      VFIGKTLQL AGWDTVGNF TKKRDGIAT VWNNSECHL 
      IYNTTQVNL KDSQMCAGG KKLPYRDQH HIDFGGILM 
      HVAKDTSYS VQGIRSIVP PCCEKDYPK VYTKVASYF 
      TWIMDNLKP
    CDS sequence
    • >TC002193 RA
      atggcacta ctcttaata tttttattt tttacaccc 
      ttaataatc acatcttat gaacattgt caaacacct 
      aaccagcaa ccaggcaaa tgtgttata ataaatcaa 
      tgcccacat ttattttct gtagttttg caaaatgca 
      acaggcgaa gaatcaaag aaacttatc caaaagtct 
      cactgcggt ttcgagtct ctcttattt cctaaagtt 
      tgttgtcct ttagaagcc atcgaactt ctagaagaa 
      agtggagca atttctcaa aataaacat tcaactaag 
      tgtggttta catgtgccc gatggacca tcggaccca 
      tggcttgcc ctattggaa tatgaacat ggtgatgga 
      cggcgtgaa tttttatgt ggtggagtg ttgattaac 
      aaaagatat gttttgaca gcagctcat tgcatcaca 
      caaaagccc aaattggga accgtccat ttaggggaa 
      tatagaagt actagtggg ttgcaatta gttattcca 
      gtggatggt actatcgta catccagag tataaaccc 
      aacgaccca catcaatac catgacatt gctttagtc 
      cgtttgaaa caaaaagtt aattttaaa gatggaatt 
      gtaaggcca atttgtgta ccaatggac agtagaaaa 
      gtgtttata gggaaaact ctgcagtta gcaggttgg 
      gacacagtt ggtaatttt acgaaaaag cgcgatggt 
      attgcaaca gtttggaac aattctgag tgtcacttg 
      atatacaac actactcaa gtgaattta aaggattct 
      cagatgtgt gccgggggt aaaaaactg ccctatagg 
      gatcaacat cacatagat tttggaggg attttaatg 
      catgttgct aaagatact tcttattcc gttcaaggg 
      attcgatcc attgtaccc ccttgttgt gaaaaagat 
      tatccaaaa gtttacact aaagtagcc agctatttc 
      acatggatt atggataat ttaaaacca taa
    mRNA sequence
    • >TC002193 RA
      cggaaacta gttgcgata gtcgcttgt tagtattca 
      gtcctcaat aatggcact actcttaat atttttatt 
      ttttacacc cttaataat cacatctta tgaacattg 
      tcaaacacc taaccagca accaggcaa atgtgttat 
      aataaatca atgcccaca tttattttc tgtagtttt 
      gcaaaatgc aacaggcga agaatcaaa gaaacttat 
      ccaaaagtc tcactgcgg tttcgagtc tctcttatt 
      tcctaaagt ttgttgtcc tttagaagc catcgaact 
      tctagaaga aagtggagc aatttctca aaataaaca 
      ttcaactaa gtgtggttt acatgtgcc cgatggacc 
      atcggaccc atggcttgc cctattgga atatgaaca 
      tggtgatgg acggcgtga atttttatg tggtggagt 
      gttgattaa caaaagata tgttttgac agcagctca 
      ttgcatcac acaaaagcc caaattggg aaccgtcca 
      tttagggga atatagaag tactagtgg gttgcaatt 
      agttattcc agtggatgg tactatcgt acatccaga 
      gtataaacc caacgaccc acatcaata ccatgacat 
      tgctttagt ccgtttgaa acaaaaagt taattttaa 
      agatggaat tgtaaggcc aatttgtgt accaatgga 
      cagtagaaa agtgtttat agggaaaac tctgcagtt 
      agcaggttg ggacacagt tggtaattt tacgaaaaa 
      gcgcgatgg tattgcaac agtttggaa caattctga 
      gtgtcactt gatatacaa cactactca agtgaattt 
      aaaggattc tcagatgtg tgccggggg taaaaaact 
      gccctatag ggatcaaca tcacataga ttttggagg 
      gattttaat gcatgttgc taaagatac ttcttattc 
      cgttcaagg gattcgatc cattgtacc cccttgttg 
      tgaaaaaga ttatccaaa agtttacac taaagtagc 
      cagctattt cacatggat tatggataa tttaaaacc 
      ataaaaatc agtttttag tattttaga tacaaagtc 
      tattaagag cctaaattt caggtaaat acacaagga 
      acaaaagtt attcaaacc tccattacg taaaataat 
      cgtaatttg aacctatgc gatccataa tccaaatta 
      ccacttaca taccttttt gtatgtctc aatttattt 
      tgtatcgat tattttgat ataagtaac gatttgtt