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Gene information
General information
TC002177 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 878116..926007
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002177
    iBeetle screen iB_00341

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002177 RA
      MGSSSGGKK KYINFRNRQ RANMSFFIV VMFFAVFGI 
      IIFTEIFLI DERGRGAGV LVRHGSLTY HHRPPARDY 
      EDAFQSDDY ISIHMGPMP NMDDTVGAL LLGRPAVIP 
      VVDDEPGPQ SSKVLTETK LPPYPAHIT PTNGSWQNV 
      NGARYKFFV FSAFFDNRR GQRSVRIIG ATKTRGPER 
      VWCRMWYKT SENNATSFI SRSVQAKIK VIRENWNLK 
      YSACFLLCP LLANQTVPT TVSVVTKVK SFPTNLLTV 
      INNYNDSMK PSNRFAVCI KPLHFDYNR ALQILEFIE 
      LNRIMGVEH FTFYNHTIG PQVGCILQD YVDRGLVTL 
      LPWQLNMLS QKEIRTEGL FAALNDCLY RSMNLYSHV 
      LLIDLDEYI IPRNNETLP QLIEYLNNR FNSRTTGAY 
      SFQNAFFYL QWGDDDSVY DSNDSVAAG LVTLKKTRR 
      RSKLHPHKQ RSKYICRPE LVVEAGNHF VWEFIPGHG 
      TMNVPSDAA ILHHYRICE FGGDDCIKT PSTVDSTAF 
      KYKNKLVDE VKEKYNEFK IKCNLMELP PPPRRVFSK 
      IINMLKSGQ KTVR
    CDS sequence
    • >TC002177 RA
      atgggctcg agcagcggg gggaagaag aagtacatc 
      aacttccgc aacagacaa agggccaac atgagtttt 
      ttcatcgtt gtgatgttt ttcgctgtg tttggaatc 
      atcatcttc actgaaatt ttcctgatt gacgagaga 
      ggaagaggc gctggagtt cttgtaagg cacggctcc 
      ctcacttac catcatcgt ccaccggcg cgagattac 
      gaagacgcc ttccagagc gatgattac atatcaatc 
      cacatgggg cccatgccc aacatggac gacaccgtc 
      ggagcactt cttctaggt cgtccggcg gtaattcca 
      gtagttgac gacgaacct ggccctcag tcttccaaa 
      gtgcttact gaaaccaag ctgccaccg tatccagca 
      cacataacc ccaacgaac ggctcctgg caaaacgtt 
      aacggcgct agatacaaa tttttcgtc ttttcggcc 
      ttctttgac aatcgcaga ggacaaaga tccgtgagg 
      ataataggc gcaacgaaa actagagga ccggaaaga 
      gtgtggtgc cggatgtgg tataaaaca agcgaaaat 
      aatgctact tcgtttatc tcacgcagc gtccaagca 
      aaaattaaa gtaattcga gaaaactgg aatctaaaa 
      tacagcgcg tgctttcta ttgtgtcct ttattggcc 
      aaccaaaca gttcctacg actgtctca gtagtcacc 
      aaagtcaaa tcattccct actaatctg ctgaccgtc 
      atcaacaac tacaacgat tcaatgaaa ccctcgaat 
      agattcgcc gtatgcatc aaaccgctc catttcgat 
      tacaacagg gcgctccaa attttggaa ttcatcgaa 
      ctgaatcgt ataatgggt gttgaacat ttcactttt 
      tacaatcac acaatcggt cctcaagtc ggatgtatt 
      ctacaagat tacgttgat cgcggttta gtcaccctg 
      ttaccgtgg caactcaac atgttgtcg caaaaggaa 
      attcgtacc gaaggcctt tttgcggcc ctcaacgat 
      tgcctttat cgctcgatg aatctctat tcgcacgtc 
      cttctcata gatctagat gagtatatc ataccaagg 
      aacaatgaa acgctaccc cagcttatc gaatatctc 
      aacaacagg tttaacagt cgcaccaca ggcgcctac 
      tcatttcaa aacgctttt ttctacctc caatggggt 
      gacgacgac tcagtttac gattcaaat gattcagtg 
      gctgccggt ttagtgact ttgaaaaag acaagacga 
      cgcagtaaa ttacatcca cacaaacaa cggtcaaag 
      tacatttgt cgaccggaa ttagttgtt gaagccgga 
      aatcatttc gtttgggag ttcattcca ggacacgga 
      acgatgaat gttccgtca gacgcagca atactacat 
      cactacaga atatgcgaa ttcggagga gacgactgt 
      ataaagaca ccttcgacg gtggactct actgcgttc 
      aaatataaa aataaacta gtggacgaa gtgaaagaa 
      aaatataac gagtttaaa atcaaatgc aatttaatg 
      gaattgcca ccacctcct cgaagggtt tttagtaag 
      attatcaat atgttaaaa agcggacaa aagacagtg 
      aggtaa
    mRNA sequence
    • >TC002177 RA
      gagtttctt tttaacaac caaacagca attcgttgc 
      atctgtgca gtttgttat cgtccctta gaacgtgaa 
      acagttcca atttttgtg aaatcctaa ttccagtct 
      gagtccgca gttttatgc aatcccatc gattgacgg 
      tccctcgta ggtatgaat gcgaaatga aacgcatgc 
      gcttgcata tccaaccct ccgaccact tccgacatt 
      gtcctttca accctaacc gcctattcc tggaaacgc 
      gtcgcgttg tgtgaagct ccgaattgt tttcttgct 
      tgaatcagc atcattcct ttaaaatat agtgtgaca 
      aagggtgca aaattacaa cataaatac tcagatcga 
      aagtcggat cttgacatg ctcctttta acactcgca 
      ccctctcca atgttgtca gctgatagt cgcgagtgg 
      taggtgctg ttttcttgc acctgaaga tcacaatgg 
      tacgcaaaa gaaggttgc aacaggcga tttaagata 
      aaacatcat gggctcgag cagcggggg gaagaagaa 
      gtacatcaa cttccgcaa cagacaaag ggccaacat 
      gagtttttt catcgttgt gatgttttt cgctgtgtt 
      tggaatcat catcttcac tgaaatttt cctgattga 
      cgagagagg aagaggcgc tggagttct tgtaaggca 
      cggctccct cacttacca tcatcgtcc accggcgcg 
      agattacga agacgcctt ccagagcga tgattacat 
      atcaatcca catggggcc catgcccaa catggacga 
      caccgtcgg agcacttct tctaggtcg tccggcggt 
      aattccagt agttgacga cgaacctgg ccctcagtc 
      ttccaaagt gcttactga aaccaagct gccaccgta 
      tccagcaca cataacccc aacgaacgg ctcctggca 
      aaacgttaa cggcgctag atacaaatt tttcgtctt 
      ttcggcctt ctttgacaa tcgcagagg acaaagatc 
      cgtgaggat aataggcgc aacgaaaac tagaggacc 
      ggaaagagt gtggtgccg gatgtggta taaaacaag 
      cgaaaataa tgctacttc gtttatctc acgcagcgt 
      ccaagcaaa aattaaagt aattcgaga aaactggaa 
      tctaaaata cagcgcgtg ctttctatt gtgtccttt 
      attggccaa ccaaacagt tcctacgac tgtctcagt 
      agtcaccaa agtcaaatc attccctac taatctgct 
      gaccgtcat caacaacta caacgattc aatgaaacc 
      ctcgaatag attcgccgt atgcatcaa accgctcca 
      tttcgatta caacagggc gctccaaat tttggaatt 
      catcgaact gaatcgtat aatgggtgt tgaacattt 
      cacttttta caatcacac aatcggtcc tcaagtcgg 
      atgtattct acaagatta cgttgatcg cggtttagt 
      caccctgtt accgtggca actcaacat gttgtcgca 
      aaaggaaat tcgtaccga aggcctttt tgcggccct 
      caacgattg cctttatcg ctcgatgaa tctctattc 
      gcacgtcct tctcataga tctagatga gtatatcat 
      accaaggaa caatgaaac gctacccca gcttatcga 
      atatctcaa caacaggtt taacagtcg caccacagg 
      cgcctactc atttcaaaa cgctttttt ctacctcca 
      atggggtga cgacgactc agtttacga ttcaaatga 
      ttcagtggc tgccggttt agtgacttt gaaaaagac 
      aagacgacg cagtaaatt acatccaca caaacaacg 
      gtcaaagta catttgtcg accggaatt agttgttga 
      agccggaaa tcatttcgt ttgggagtt cattccagg 
      acacggaac gatgaatgt tccgtcaga cgcagcaat 
      actacatca ctacagaat atgcgaatt cggaggaga 
      cgactgtat aaagacacc ttcgacggt ggactctac 
      tgcgttcaa atataaaaa taaactagt ggacgaagt 
      gaaagaaaa atataacga gtttaaaat caaatgcaa 
      tttaatgga attgccacc acctcctcg aagggtttt 
      tagtaagat tatcaatat gttaaaaag cggacaaaa 
      gacagtgag gtaattctt aaatgtacc taataagat 
      attttttta agaacattt gtattattt tgtatataa 
      ttgtgtatt ctgatttgt aaataggac aagttgcac 
      tggaactat atcaacaac attatcatt tggtcgtaa 
      gtgtataaa ggctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY