General information | |||
TC030942
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450989.1: 133045..148046 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC030942 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00338 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00321 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
pupation delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
remarks: animals lost, larva lethality late (L7) - Number of animals showing the phenotype: 4 (19 dpi)
- >TC030942 RA
MEQVAALKD KGNAALAAN KFEEAIKHY TEAIKLDPS NHVLYSNRS AAFAKANNH ESALEDANK TVELKPDWA KGYSRKGAA LAYLGRLDE AIATYERGL QIEPANPQL QEGLQEVKA QKTAKGFPN PFNRPDLME KLRGDPRTK PLLDDPNYV ATLHMLKTN PNLLGQKLQ DPNILTTLS VLIGIDPGE EPMETEPVY TPPKKPEPK KEPEPDLPE NKKLAKAAK EQGNEFYKK KNFEKAIEF YNQAIEHDP TDITFYNNL AAVFFEQKD YDRCIKECE RAIDIGREN RADFKLIAK SFLRIGNAY KRLKDYQNA KIYYEKSMS EHRTPEIKT LLSDVEKII KEEERKAYV NPELAEKEK EIGNELFKK GDYATAVKH YTEAIKRNP EDAKLYSNR AACYTKLAA FDLGLKDCD KCVELDPKF IKGWIRKAH ILQGMQQPT KAMSAFQKA LEIDPNNAE AINGYRACS IENASGSGD PEKIRQRAM ADPEVQSIL RDPAMRMIL EQMQNDPRA LQDHLKNPD IAAKIQKLL ESGLIAIH
- >TC030942 RA
atggaacaa gtcgccgct ttgaaagac aaaggcaat gccgcactg gccgccaac aagttcgaa gaggcaatc aaacactac accgaagcc atcaaactc gacccctcg aaccacgtc ctgtactcc aaccgttcc gccgccttc gccaaagcc aacaaccac gaatcggct ctcgaagac gccaacaaa acggtcgaa ctgaagccc gactgggcc aagggctac tcacggaag ggggctgcc ttggcatac ctggggcgc ctcgacgag gccatcgcc acgtacgag cgcggcttg cagatcgag cccgccaac ccccagctc caggagggg ctgcaggag gtcaaagcc cagaaaaca gccaagggg ttccccaat cctttcaac cgacctgac ctcatggag aagcttcgc ggggacccc cggaccaag cccctgctc gatgacccc aactacgtc gcgacgctc cacatgctc aaaacaaac cccaatctc ctcggccag aaactacaa gaccccaat attttgacc acgttgagt gtcttgatt ggaatcgat ccgggggaa gaaccgatg gagaccgaa ccggtgtac acgccaccg aagaagccc gaaccgaag aaagaaccc gagccggac ttacccgag aacaagaaa ctggcgaaa gcggcgaag gaacaaggg aatgaattt tacaaaaag aagaatttt gaaaaagcg atcgagttt tataatcag gcgattgaa cacgatccc accgatatc acgttttat aataatttg gcggcggtt tttttcgaa cagaaggat tacgatcga tgtattaag gagtgcgag agggctatt gatataggg agggagaat agagccgat tttaaatta attgcaaag tcgtttctt aggattggg aatgcgtat aagagactg aaggattat caaaatgcg aaaatttat tacgaaaag tcaatgtcc gaacatagg acgcctgaa attaaaacg ctgttgtcc gatgttgag aagattatc aaagaggag gaacggaaa gcttatgtt aatcccgaa ttagctgaa aaagagaag gaaattggt aatgaatta tttaagaaa ggtgattac gccactgcc gtcaaacac tacaccgaa gccatcaaa cgcaaccca gaagacgcc aaactgtac agtaaccgt gctgcctgt tatacaaaa ctagctgcc tttgatctt ggtttaaaa gactgcgac aagtgcgtt gagctcgat ccgaaattc atcaaaggg tggatccga aaagcacac attttgcaa ggaatgcaa caaccgacc aaagccatg tcagctttc caaaaagcg ctcgaaatc gatccgaat aatgccgaa gctattaac ggatataga gcatgcagt atagaaaat gcgagcggt tcaggtgat cctgaaaag ataagacaa cgtgcgatg gctgatcct gaagttcag tcgattttg agagatccc gcaatgagg atgatcttg gaacagatg caaaatgat ccaagagct ttgcaagac cacttaaag aatcctgat atagctgca aagatacaa aagttgcta gagtcgggg cttattgct attcattaa
- >TC030942 RA
gtgtttttg tgattatta agtgccggt caaaatgga acaagtcgc cgctttgaa agacaaagg caatgccgc actggccgc caacaagtt cgaagaggc aatcaaaca ctacaccga agccatcaa actcgaccc ctcgaacca cgtcctgta ctccaaccg ttccgccgc cttcgccaa agccaacaa ccacgaatc ggctctcga agacgccaa caaaacggt cgaactgaa gcccgactg ggccaaggg ctactcacg gaagggggc tgccttggc atacctggg gcgcctcga cgaggccat cgccacgta cgagcgcgg cttgcagat cgagcccgc caaccccca gctccagga ggggctgca ggaggtcaa agcccagaa aacagccaa ggggttccc caatccttt caaccgacc tgacctcat ggagaagct tcgcgggga cccccggac caagcccct gctcgatga ccccaacta cgtcgcgac gctccacat gctcaaaac aaaccccaa tctcctcgg ccagaaact acaagaccc caatatttt gaccacgtt gagtgtctt gattggaat cgatccggg ggaagaacc gatggagac cgaaccggt gtacacgcc accgaagaa gcccgaacc gaagaaaga acccgagcc ggacttacc cgagaacaa gaaactggc gaaagcggc gaaggaaca agggaatga attttacaa aaagaagaa ttttgaaaa agcgatcga gttttataa tcaggcgat tgaacacga tcccaccga tatcacgtt ttataataa tttggcggc ggttttttt cgaacagaa ggattacga tcgatgtat taaggagtg cgagagggc tattgatat agggaggga gaatagagc cgattttaa attaattgc aaagtcgtt tcttaggat tgggaatgc gtataagag actgaagga ttatcaaaa tgcgaaaat ttattacga aaagtcaat gtccgaaca taggacgcc tgaaattaa aacgctgtt gtccgatgt tgagaagat tatcaaaga ggaggaacg gaaagctta tgttaatcc cgaattagc tgaaaaaga gaaggaaat tggtaatga attatttaa gaaaggtga ttacgccac tgccgtcaa acactacac cgaagccat caaacgcaa cccagaaga cgccaaact gtacagtaa ccgtgctgc ctgttatac aaaactagc tgcctttga tcttggttt aaaagactg cgacaagtg cgttgagct cgatccgaa attcatcaa agggtggat ccgaaaagc acacatttt gcaaggaat gcaacaacc gaccaaagc catgtcagc tttccaaaa agcgctcga aatcgatcc gaataatgc cgaagctat taacggata tagagcatg cagtataga aaatgcgag cggttcagg tgatcctga aaagataag acaacgtgc gatggctga tcctgaagt tcagtcgat tttgagaga tcccgcaat gaggatgat cttggaaca gatgcaaaa tgatccaag agctttgca agaccactt aaagaatcc tgatatagc tgcaaagat acaaaagtt gctagagtc ggggcttat tgctattca ttaattttt ggtttagtg atgaggaac gtgcgatta tgtttgtgt tgaatagcg atcgaagcg catttagag tggttgcag tatccagcg tttgatctt tttacttag cttaataac cgtttaagt aataaattt acgttttta aagcacatt tctatttat ttacttacc aacaagtct gaccacagt gccctctac ttattgatt ataacttat aagtattgt attcatttc ttttgtaat cattaaatt acgcttcaa ata
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
