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Gene information
General information
TC002162 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 12247..76379
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002162
    iBeetle screen iB_00337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002162 RA
      MASNDETLD DHQRFVLMK FRRNVSDIT QPHHDDKFL 
      LRWLRARSW DAEAAEKML RQSMKWRQQ WEVDGALKN 
      WQPSESLLN FYPCGVSGY DKDGAPVII VPFGGLDMV 
      GILHAFGRN DLIKLTIQT LERFMELAA EKGGHKFVV 
      IFDMDAFNI RQYAWRPAA EVVVSLVQM YEANYPEIL 
      KACYIINAP RVFAIAFNV IKRFLNEYT LGKIQIFKN 
      DPKKWKKAV LANIEPDNL PEHFGGTLA DPDGNPRYT 
      TKVVHSLVH PEILKACYI INAPRVFAI AFNVIKRFL 
      NEYTLGKIQ IFKNDPKKW KKAVLANIE PDNLPEHFG 
      GTLADPDGN PRYTTKINQ GGKVPKELY KTTFDISST 
      TRQFTTAVI KKGEKLKLD FIVVEEGSF LKWEFRTEA 
      HDIRFGISL IDAEGNVTP VIHHKRVAA HQIDESGVI 
      ACQAPATYT VTFDNTYSL LRSKKIHYE IHIAPPLGK 
      LDILPNEDE INGNVKSEI ATTSAQ
    CDS sequence
    • >TC002162 RA
      atggcttca aacgatgaa actttggac gaccaccag 
      agatttgtg ttgatgaag tttcgtcga aacgtatcc 
      gacatcaca caaccacac catgacgat aaatttctc 
      ctgcgatgg ctaagagct cgttcctgg gatgcggaa 
      gcagcggaa aaaatgctc agacagtcg atgaaatgg 
      cgtcagcaa tgggaagtc gatggtgct ttaaaaaac 
      tggcaaccg tccgaaagt ctcctcaat ttttatccg 
      tgtggcgtt agcggttac gacaaagac ggagctcct 
      gtgataatt gttcctttc ggtggtcta gatatggtc 
      ggaattttg cacgcattt ggacgcaac gacttgatt 
      aaattaact atacagacg ttggaaagg ttcatggag 
      ttggccgcc gagaaagga ggccataaa ttcgtggtc 
      atattcgat atggacgcg tttaatatt cgacaatac 
      gcctggcga ccagctgct gaagttgtc gtatcttta 
      gtccaaatg tacgaagca aactatccc gaaattttg 
      aaagcttgc tacattata aacgcgcca agagttttc 
      gcaattgca ttcaatgtg ataaagagg tttttgaac 
      gagtatacg ctagggaag atacaaatt ttcaaaaat 
      gatccgaaa aaatggaaa aaggcagtg cttgctaat 
      attgaaccc gataatttg cccgaacat tttgggggg 
      actttagca gatccggat gggaatccc cgatacaca 
      acaaaggtg gttcactct ttggtgcat cccgaaatt 
      ttgaaagct tgctacatt ataaacgcg ccaagagtt 
      ttcgcaatt gcattcaat gtgataaag aggtttttg 
      aacgagtat acgctaggg aagatacaa attttcaaa 
      aatgatccg aaaaaatgg aaaaaggca gtgcttgct 
      aatattgaa cccgataat ttgcccgaa cattttggg 
      gggacttta gcagatccg gatgggaat ccccgatac 
      acaacaaag ataaaccaa ggcggtaaa gtccccaaa 
      gaactatac aaaacaacg ttcgacatt tcgtcaacc 
      actcgacaa ttcacaaca gctgtcatc aaaaaaggc 
      gaaaagtta aaacttgat tttatcgta gttgaggaa 
      ggaagcttc ttaaaatgg gaatttcgc acagaggca 
      cacgatatt cgcttcggc ataagttta atagatgcc 
      gaaggcaac gtcactcca gttatccat cacaaacgc 
      gtggcagca catcagata gacgaaagc ggtgtcata 
      gcgtgccaa gcaccagcc acatatact gtgactttt 
      gacaacacc tacagtctt ctaagaagc aaaaaaatc 
      cactatgaa attcacatc gcacctcca ttgggcaaa 
      cttgatatt ttaccaaac gaagatgaa attaacgga 
      aatgtgaag agtgaaatt gcaacaaca agcgcccaa 
      taa
    mRNA sequence
    • >TC002162 RA
      tcgaaccca ctgtaaatg aatactaaa atctgaaca 
      ctctgtgaa atacatgtg tagtgtcca tcacttagt 
      gttgttctg aagtgcaat ggcttcaaa cgatgaaac 
      tttggacga ccaccagag atttgtgtt gatgaagtt 
      tcgtcgaaa cgtatccga catcacaca accacacca 
      tgacgataa atttctcct gcgatggct aagagctcg 
      ttcctggga tgcggaagc agcggaaaa aatgctcag 
      acagtcgat gaaatggcg tcagcaatg ggaagtcga 
      tggtgcttt aaaaaactg gcaaccgtc cgaaagtct 
      cctcaattt ttatccgtg tggcgttag cggttacga 
      caaagacgg agctcctgt gataattgt tcctttcgg 
      tggtctaga tatggtcgg aattttgca cgcatttgg 
      acgcaacga cttgattaa attaactat acagacgtt 
      ggaaaggtt catggagtt ggccgccga gaaaggagg 
      ccataaatt cgtggtcat attcgatat ggacgcgtt 
      taatattcg acaatacgc ctggcgacc agctgctga 
      agttgtcgt atctttagt ccaaatgta cgaagcaaa 
      ctatcccga aattttgaa agcttgcta cattataaa 
      cgcgccaag agttttcgc aattgcatt caatgtgat 
      aaagaggtt tttgaacga gtatacgct agggaagat 
      acaaatttt caaaaatga tccgaaaaa atggaaaaa 
      ggcagtgct tgctaatat tgaacccga taatttgcc 
      cgaacattt tggggggac tttagcaga tccggatgg 
      gaatccccg atacacaac aaaggtggt tcactcttt 
      ggtgcatcc cgaaatttt gaaagcttg ctacattat 
      aaacgcgcc aagagtttt cgcaattgc attcaatgt 
      gataaagag gtttttgaa cgagtatac gctagggaa 
      gatacaaat tttcaaaaa tgatccgaa aaaatggaa 
      aaaggcagt gcttgctaa tattgaacc cgataattt 
      gcccgaaca ttttggggg gactttagc agatccgga 
      tgggaatcc ccgatacac aacaaagat aaaccaagg 
      cggtaaagt ccccaaaga actatacaa aacaacgtt 
      cgacatttc gtcaaccac tcgacaatt cacaacagc 
      tgtcatcaa aaaaggcga aaagttaaa acttgattt 
      tatcgtagt tgaggaagg aagcttctt aaaatggga 
      atttcgcac agaggcaca cgatattcg cttcggcat 
      aagtttaat agatgccga aggcaacgt cactccagt 
      tatccatca caaacgcgt ggcagcaca tcagataga 
      cgaaagcgg tgtcatagc gtgccaagc accagccac 
      atatactgt gacttttga caacaccta cagtcttct 
      aagaagcaa aaaaatcca ctatgaaat tcacatcgc 
      acctccatt gggcaaact tgatatttt accaaacga 
      agatgaaat taacggaaa tgtgaagag tgaaattgc 
      aacaacaag cgcccaata ataattatt ataattaaa 
      acaattggt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY