Status
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Gene information
General information
TC002149 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20394518..20401430
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002149
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00336

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002149 RA
      MSDTREKYG PLIGSIDEG TSSTRFLVF ASKTAEVLT 
      YHQVKIPHI TPKEGWFEQ DPMQILNSV IETINVTCD 
      NLKKLNINY EDIVATGIT NQRETTILW DRTTGKPLY 
      NALVWMDMR TSSTVDSIL LNGKRNKNF LQSMCGLPI 
      STYFSALKI KWLMDNVDG VKKAMEEKR CLFGTVDTW 
      LIWNLTGGV KGGLHITDV TNASRTMLM NIETLKWDS 
      YLCKVFDIP MHILPEIRS SSEIYGYIT DVTVLAGIP 
      ISGVLGDQQ AALVGQMCF HQGQAKNTY GTGCFLLYN 
      TGTTMVQSN HGLLTTVGY KLGPDKPTV YALEGSIAI 
      AGISIKWLQ DNLMILKDS KDSGDIAAS ADEVGEVYF 
      VPAFSGLYA PYWRKDARS VICGLTEET KPEHLVKAA 
      LEAVCYQVR DILEAMALD CGYSLSKLL VDGGMTAND 
      FLMQMQADY CGIPVVRPT MAETTCLGA AIAAGNAKG 
      VEVWDLDNI QPVPSDTFL PSISDNQRD IKYARWKMA 
      INRSLGWAQ DEEEDNSFE DDNMLTTLP GGLYVLGTM 
      VMLLVAEIL EKK
    CDS sequence
    • >TC002149 RA
      atgtctgat acaagagaa aaatatggc ccactaatc 
      ggatcaata gatgaagga acttcaagc acaaggttt 
      ttggtcttt gcttccaaa acagcagag gtattgacc 
      tatcaccag gttaaaatc ccacacatt actccaaag 
      gagggatgg tttgagcaa gatcctatg cagattttg 
      aattctgtg atagagacc atcaatgta acttgtgat 
      aacttaaag aaacttaat attaattat gaggatata 
      gtggctacg ggtattacc aaccaaaga gagaccaca 
      attctttgg gaccgcaca actggcaaa ccattatat 
      aacgcttta gtatggatg gatatgcga acttcaagc 
      acagtagac agtattttg ttaaatgga aagagaaat 
      aagaatttt ttacaaagt atgtgtggt ctccctatc 
      agcacatac tttagtgct ttaaaaatc aagtggttg 
      atggataac gttgatggg gttaagaaa gcaatggag 
      gagaaaaga tgtcttttt ggaacggtt gacacttgg 
      ttgatttgg aatctaaca ggtggtgta aaaggtggc 
      ctacatata actgatgtt accaatgct tcaagaaca 
      atgttgatg aatatagaa acacttaaa tgggattct 
      tatttatgt aaagttttt gacattccg atgcacatt 
      ttgcctgaa attcgaagt tcttctgag atatatggc 
      tacataact gatgttaca gtgttggct ggaattcca 
      atttcgggg gtgttgggt gatcaacag gcggcttta 
      gttggccaa atgtgtttt caccaaggt caagccaaa 
      aatacatat ggcactggc tgttttctc ttgtataat 
      accggaact acgatggtt cagtcaaac catggtctt 
      ttaacaaca gttggttat aaattaggc cctgataaa 
      ccaactgtt tatgccttg gaaggatca attgctatt 
      gcaggcatt tcgataaaa tggcttcag gacaattta 
      atgattttg aaagactcg aaagactct ggagacatt 
      gccgcatct gctgacgaa gtgggtgaa gtttatttt 
      gtgccagcg ttctctggt ctttatgca ccttattgg 
      cgtaaagat gctagaagt gttatatgt ggtctgact 
      gaggaaaca aagccagaa catttagtc aaagctgct 
      ttagaagca gtctgttac caagtaagg gatatttta 
      gaagcaatg gcgttagac tgtggttac tctttatct 
      aaacttcta gtagacggt ggtatgaca gctaatgat 
      tttttaatg caaatgcaa gctgattat tgtggaatt 
      cctgtggtg cggcctact atggctgaa actacttgt 
      ttaggggca gctatcgca gctggaaat gcaaaaggg 
      gttgaagtg tgggatttg gataatata caacctgtg 
      ccaagcgat acattctta ccgtcaatt tctgacaat 
      caacgggat ataaaatat gcacggtgg aaaatggca 
      attaatcga agtttaggg tgggcacag gatgaggaa 
      gaagacaat agttttgaa gatgacaat atgctaaca 
      acactgcca ggaggactt tacgtactt ggaactatg 
      gttatgcta cttgtggct gaaattctg gaaaagaaa 
      tag
    mRNA sequence
    • >TC002149 RA
      aggctgtcg aagaatttt gctcctttt tctttgaac 
      ggttgttta aattatttg tgttaggtg tgactatta 
      cgtgttaat tactgttgt tggtgggtg gtggttgaa 
      gaaaaagaa acacgtttt tccgaaagt gttacccac 
      aaatttgaa atacataaa gtgcaccac tggttcaaa 
      cattaatgc agttattaa gttttaaat attttatct 
      ttctctacc acctaccat tgagttact gccggacat 
      tttgaacat tgtacaaaa cgtggaata cccttattg 
      ttttttttt tttgttttc tacactctc actgacgtg 
      acgaaacaa agtcatttc attacttga attcaaaca 
      agttagacc atttattag tttattatt ttctgttgc 
      tatgacacc ttcatataa atattgtct tgttcctac 
      ttgtaaaaa cacgacaag taccacacc tgataagta 
      tttctttcc tgtaccaag agataaaaa actaagaaa 
      agaatggtg tatagcgat gaaaagaaa cgataatca 
      actctcttc taaaacatc gctacactg ttgtgaaga 
      ccacttcga tataaaagc ggatgtaga aaactatgt 
      caaatatag cttagttgg gggaaaaat gacgttgaa 
      gaatggagt tttgcctca caaatttga ctgtcagac 
      agagttttt ggaagcatt cttaccaga aactgatgt 
      tagtgaatc tctcgaaaa accatcatt tgagtttgt 
      gtcttgtgg tattagtgc gtccgaatt gtaataaat 
      tagctcttt tatatcatt tttacattg accaaaaca 
      cttaaaaat aggtatagg tttttgcaa acaatgaaa 
      atttatggt ttaagatga tttatttac attttgttg 
      ttttatgga tagccaaaa tttagtttt tttttataa 
      aataaacga tccatttac attataacc acttatttt 
      atattaagt agacagtac aattgcgca tagagtttc 
      ttataactc ataaaatcc gtggccatt ttaattttt 
      ttgggcgcc ctctacttt ttgtcgcta ggagcttcc 
      cgttttaca gttggcagc agcaacact gcgtaaaat 
      ttccaatgt cagatgtga tgcgaaagc aagtgcggc 
      aaataaata accacaatt taaaattaa aattcttcg 
      actattgta ataccaacg aataatttt gcatataat 
      gttaaaact gtgaggata ttacgaaag tcatgtctg 
      atacaagag aaaaatatg gcccactaa tcggatcaa 
      tagatgaag gaacttcaa gcacaaggt ttttggtct 
      ttgcttcca aaacagcag aggtattga cctatcacc 
      aggttaaaa tcccacaca ttactccaa aggagggat 
      ggtttgagc aagatccta tgcagattt tgaattctg 
      tgatagaga ccatcaatg taacttgtg ataacttaa 
      agaaactta atattaatt atgaggata tagtggcta 
      cgggtatta ccaaccaaa gagagacca caattcttt 
      gggaccgca caactggca aaccattat ataacgctt 
      tagtatgga tggatatgc gaacttcaa gcacagtag 
      acagtattt tgttaaatg gaaagagaa ataagaatt 
      ttttacaaa gtatgtgtg gtctcccta tcagcacat 
      actttagtg ctttaaaaa tcaagtggt tgatggata 
      acgttgatg gggttaaga aagcaatgg aggagaaaa 
      gatgtcttt ttggaacgg ttgacactt ggttgattt 
      ggaatctaa caggtggtg taaaaggtg gcctacata 
      taactgatg ttaccaatg cttcaagaa caatgttga 
      tgaatatag aaacactta aatgggatt cttatttat 
      gtaaagttt ttgacattc cgatgcaca ttttgcctg 
      aaattcgaa gttcttctg agatatatg gctacataa 
      ctgatgtta cagtgttgg ctggaattc caatttcgg 
      gggtgttgg gtgatcaac aggcggctt tagttggcc 
      aaatgtgtt ttcaccaag gtcaagcca aaaatacat 
      atggcactg gctgttttc tcttgtata ataccggaa 
      ctacgatgg ttcagtcaa accatggtc ttttaacaa 
      cagttggtt ataaattag gccctgata aaccaactg 
      tttatgcct tggaaggat caattgcta ttgcaggca 
      tttcgataa aatggcttc aggacaatt taatgattt 
      tgaaagact cgaaagact ctggagaca ttgccgcat 
      ctgctgacg aagtgggtg aagtttatt ttgtgccag 
      cgttctctg gtctttatg caccttatt ggcgtaaag 
      atgctagaa gtgttatat gtggtctga ctgaggaaa 
      caaagccag aacatttag tcaaagctg ctttagaag 
      cagtctgtt accaagtaa gggatattt tagaagcaa 
      tggcgttag actgtggtt actctttat ctaaacttc 
      tagtagacg gtggtatga cagctaatg attttttaa 
      tgcaaatgc aagctgatt attgtggaa ttcctgtgg 
      tgcggccta ctatggctg aaactactt gtttagggg 
      cagctatcg cagctggaa atgcaaaag gggttgaag 
      tgtgggatt tggataata tacaacctg tgccaagcg 
      atacattct taccgtcaa tttctgaca atcaacggg 
      atataaaat atgcacggt ggaaaatgg caattaatc 
      gaagtttag ggtgggcac aggatgagg aagaagaca 
      atagttttg aagatgaca atatgctaa caacactgc 
      caggaggac tttacgtac ttggaacta tggttatgc 
      tacttgtgg ctgaaattc tggaaaaga aatagaaag 
      ttaaatttt ctttgtttt taaagtaca aagaactga 
      aacaagtca aactaataa gaagaattt aagtgtgat 
      ttgtgattt ttgttacgg ttgtgcttt gagtgaact 
      atttgcatt taaaatcac cttactttg ctgtgattt 
      ttttgttgt ggtttttat agtattgtt tatggctta 
      tggtaacaa aagtattgt gatttttat gcgattggt 
      aatacatac tttggagca at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY