Status
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Gene information
General information
TC002137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19959030..19964558
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002137
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00333

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002137 RA
      MADEYSSWV GLIYVFNLI VGTGALTLP SVFAGAGWL 
      LSTILVSLL ALVSFITVT FVIETMACA NATVHWRRV 
      QSHKTDESE VPADSDSEP ENTTEETAI MSINRRRKR 
      YYNLNTKVE LGEMAKLYF NKFGQLLFY VSLCIYLYG 
      DLAIYATAV SKTIVDLTC KSYNNDTED YSTKCSDSL 
      NVAKIDMYR IFVAIFALS VGPFTYFKV QKTKYLQIV 
      TTALRWSAF VIMITLACI RLSKNGQEG HPDLVHLSG 
      VPSLIGSSI YSFMCHHSV PGLIAPFAN KQHVIRQIG 
      LDYLSICCF YLLLAMTGS FAFETLDDL YTLNFIPTE 
      SDDSGVFME VIKYFLGAF PLFTLSTSF PIIAITLQN 
      NLKSLFLDI NVMERYNFF VRRLAFPTL AVVPPIVVA 
      FSTHNLRTL VAFTGSYAG VLIQYIIPA CLVYFARQH 
      CARNIGNYD NKYSSPFRH VFWFTFVIG WSIFCVIFV 
      TANFIMQEI HTK
    CDS sequence
    • >TC002137 RA
      atggctgat gaatattcg tcatgggtg ggtttgatt 
      tacgttttc aatttaatt gtgggcact ggtgccctt 
      actctcccc tcggtgttt gccggggcg ggatggctc 
      ttaagtaca attttagtg agtctttta gcactggtc 
      agtttcata acagtgact tttgttatt gaaacgatg 
      gcttgtgct aacgccaca gtccattgg agacgcgtc 
      cagtctcac aaaactgat gaaagtgaa gtacctgct 
      gacagtgat tccgaacct gagaacaca accgaagaa 
      accgccatt atgagcata aaccgtcga aggaagcgc 
      tactataat ctaaataca aaggtagaa ttaggcgaa 
      atggccaaa ctctatttt aataaattc ggccagttg 
      ttgttttac gtctcactg tgtatttat ctatacggt 
      gacttagct atttacgcc acagccgtc tcgaaaaca 
      atagtggac ctcacctgt aagtcgtac aataacgat 
      acagaagat tatagtact aagtgttcc gattcgtta 
      aatgttgca aaaattgac atgtatcgt atatttgtg 
      gccattttt gcgttatcg gttggtcca ttcacttat 
      tttaaagtc caaaaaacc aaatatctc cagattgtt 
      acaacagcc ctgcgatgg tcagcgttt gttataatg 
      atcacgctg gcgtgtatc agattgtca aaaaatgga 
      caagaaggc caccctgat ttggttcat ttgagcggt 
      gttccgagc ctgattggc tcaagtatt tatagtttt 
      atgtgccat cattcggtt cctggtcta atagcgcct 
      tttgccaat aagcagcat gttattcgc caaatcggt 
      ttggattat ttgtcgatc tgctgtttt tatttgttg 
      ttagcaatg actggttct tttgcgttt gaaactttg 
      gatgacttg tatacgctc aattttatt ccaacggaa 
      tcagacgat tctggcgtt tttatggaa gtcataaag 
      tattttctg ggagctttt cccttgttt acgctttct 
      acgagcttc ccaattatc gcaattacg ctacagaat 
      aatttaaag tccctgttt cttgatatt aacgtcatg 
      gagagatat aattttttt gtgcggaga ctagcgttc 
      cctacactt gcggtggtt cccccgatt gttgtggcg 
      ttttctacg cacaatttg aggactttg gtggcgttt 
      acaggctca tatgctggc gttttaata cagtatatt 
      ataccagct tgtttggtg tattttgct cgccaacat 
      tgcgcaaga aatatcggg aattatgac aataaatat 
      tctagtcca tttagacac gtcttttgg ttcactttt 
      gtgataggt tggtcaata ttttgcgtt atttttgtc 
      acggccaat tttattatg caggaaata catacaaaa 
      tag
    mRNA sequence
    • >TC002137 RA
      aaagcaaaa acagaaatt tgaacatgt aattttgtg 
      atttaactt aaagccaca ctatcaaaa tagtgttta 
      ggaatgtta cggaacatt acgttttaa cagaacgat 
      caaattgta ttagggatg gctgatgaa tattcgtca 
      tgggtgggt ttgatttac gttttcaat ttaattgtg 
      ggcactggt gcccttact ctcccctcg gtgtttgcc 
      ggggcggga tggctctta agtacaatt ttagtgagt 
      cttttagca ctggtcagt ttcataaca gtgactttt 
      gttattgaa acgatggct tgtgctaac gccacagtc 
      cattggaga cgcgtccag tctcacaaa actgatgaa 
      agtgaagta cctgctgac agtgattcc gaacctgag 
      aacacaacc gaagaaacc gccattatg agcataaac 
      cgtcgaagg aagcgctac tataatcta aatacaaag 
      gtagaatta ggcgaaatg gccaaactc tattttaat 
      aaattcggc cagttgttg ttttacgtc tcactgtgt 
      atttatcta tacggtgac ttagctatt tacgccaca 
      gccgtctcg aaaacaata gtggacctc acctgtaag 
      tcgtacaat aacgataca gaagattat agtactaag 
      tgttccgat tcgttaaat gttgcaaaa attgacatg 
      tatcgtata tttgtggcc atttttgcg ttatcggtt 
      ggtccattc acttatttt aaagtccaa aaaaccaaa 
      tatctccag attgttaca acagccctg cgatggtca 
      gcgtttgtt ataatgatc acgctggcg tgtatcaga 
      ttgtcaaaa aatggacaa gaaggccac cctgatttg 
      gttcatttg agcggtgtt ccgagcctg attggctca 
      agtatttat agttttatg tgccatcat tcggttcct 
      ggtctaata gcgcctttt gccaataag cagcatgtt 
      attcgccaa atcggtttg gattatttg tcgatctgc 
      tgtttttat ttgttgtta gcaatgact ggttctttt 
      gcgtttgaa actttggat gacttgtat acgctcaat 
      tttattcca acggaatca gacgattct ggcgttttt 
      atggaagtc ataaagtat tttctggga gcttttccc 
      ttgtttacg ctttctacg agcttccca attatcgca 
      attacgcta cagaataat ttaaagtcc ctgtttctt 
      gatattaac gtcatggag agatataat ttttttgtg 
      cggagacta gcgttccct acacttgcg gtggttccc 
      ccgattgtt gtggcgttt tctacgcac aatttgagg 
      actttggtg gcgtttaca ggctcatat gctggcgtt 
      ttaatacag tatattata ccagcttgt ttggtgtat 
      tttgctcgc caacattgc gcaagaaat atcgggaat 
      tatgacaat aaatattct agtccattt agacacgtc 
      ttttggttc acttttgtg ataggttgg tcaatattt 
      tgcgttatt tttgtcacg gccaatttt attatgcag 
      gaaatacat acaaaatag ttattattt tatacttgt 
      accaggtga atcaacttt tatccgctg tcatactat 
      ttagtgtga aaactcgga aaatgtgta tgctaggga 
      gaaataaat gctaggagg caaaataac gagattttc 
      tgtacccaa aaatttagt acaggggaa caaaaatga 
      gggcgcttc gagccctag ttcactagt tcaatttgt 
      tcactaatt gcgttgagt atcaagctg agatatact 
      gatgactaa agtttttac tttacttaa attatagaa 
      ttatttaaa attattaga gaaatgtaa aaagtataa 
      tttttttat ttcaatgct ctaaaattt aaagttatg 
      taaagagca cgataaggg atataatga taaaatgta 
      taatacaat ggtattttt ttgaaattt atacaaagt 
      aaaaattat aagctataa acattaagc gtaaaaaaa 
      aactcaaag gagtaaaat gcatttgtt tatagtttt 
      tactcctat tatttattt tgttacacg ttgatttca 
      tatagctag cacatagct cccaagaag gaatttcgt 
      atacgagta aataaaaaa taatcaaaa agtaagtaa 
      aaaatcgtg aattagaaa aacgtttaa acttaaaag 
      gttgacttg ggagcgtta acaactcgt ttttttata 
      tttattcac ttatgtaca cttatttta cacttattg 
      actattaac acttctaca ataattatc acactttga 
      atacaaata tttttgttt tattttatt ttttattgt 
      aactttatt ttgacgata atataatat aaaaaatag 
      agaaaccgt ttttaaaaa ctttttaca taatgttaa 
      tttaaatta ttacaagtt ttgtgaaca atcaaagaa 
      atacaaaat ctgtacaca ttgtaggtt ttaacaaca 
      aacattaaa catttattt tgtgctaaa ataacatat 
      tacaatctt gtacatacg ttaacagaa tacatggaa 
      taattagta tagacgaaa ctaaacaga tgctaagga 
      aaatatttt tctgtatta ctattttaa aaatcactt 
      ttaagtagt tttaagaca agcattcag tggttttag 
      aaaacaatg acattttcg tgccataat acctaaaat 
      ttcaagatc ttcatgctc aatatttaa agattcata 
      aaaaacatg tcaataatt atcaattgt tctaaattt 
      ttttcaagt agttttatt ttaccgctt gagttggaa 
      tgttggatc caataaatt actaataca aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY